LOCUS       NZ_CP049194             5976 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain Y2152 plasmid pDC2152-6, complete
            sequence.
ACCESSION   NZ_CP049194
VERSION     NZ_CP049194.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14151378
            Assembly: GCF_011045355.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Zhu,X. and Bao,Q.
  TITLE     Prevalence of Aminoglycoside Resistance Genes and Molecular
            Characterization of a Novel Gene aac(3)-IIg among Clinical Isolates
            of Enterobacter cloacae Complex from a Chinese Teaching Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   Zhu,X. and Bao,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2020) Laboratory Medicine, College of Life
            Science, Wenzhou Medical University, Wenzhou Medical University,
            Wenzhou, Zhejiang 325000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. v1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011045355.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:25:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,892
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,587
            CDSs (with protein)               :: 4,587
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 191
            CDSs (without protein)            :: 191
            Pseudo Genes (ambiguous residues) :: 0 of 191
            Pseudo Genes (frameshifted)       :: 120 of 191
            Pseudo Genes (incomplete)         :: 68 of 191
            Pseudo Genes (internal stop)      :: 26 of 191
            Pseudo Genes (multiple problems)  :: 23 of 191
            Pseudo Genes (short protein)      :: 1 of 191
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049194.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Y2152"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pDC2152-6"
                     /geo_loc_name="China:Wenzhou"
                     /collection_date="2007-01-31"
     gene            408..731
                     /locus_tag="G5577_RS24250"
                     /old_locus_tag="G5577_24255"
     CDS             408..731
                     /locus_tag="G5577_RS24250"
                     /old_locus_tag="G5577_24255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_029593203.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            940..>1380
                     /locus_tag="G5577_RS24695"
                     /pseudo=""
     CDS             940..>1380
                     /locus_tag="G5577_RS24695"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1409..1906
                     /locus_tag="G5577_RS24260"
                     /old_locus_tag="G5577_24265"
     CDS             1409..1906
                     /locus_tag="G5577_RS24260"
                     /old_locus_tag="G5577_24265"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_164474471.1"
                     /translation="MSNLFTASRDFEEKLKAQQQSTGEMLKAAFSEHEQSVGAELSASA
                     RRISDAILAHELSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIGASGSILWWQGQQIT
                     DNYTTLRQQEDALAKMSAKTWGVSYQSDSNGRFLVLPKGAKAETGWTVDSGKRNAVKLV
                     QE"
                     /name="MbeB family mobilization protein"
     gene            1913..2128
                     /locus_tag="G5577_RS24265"
                     /old_locus_tag="G5577_24270"
     CDS             1913..2128
                     /locus_tag="G5577_RS24265"
                     /old_locus_tag="G5577_24270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_164474472.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFEEWRRMSGLMQRENA
                     MLNERVSGLSRQVQSLSEQLRRLSGS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2309..2815
                     /locus_tag="G5577_RS24270"
                     /old_locus_tag="G5577_24275"
     CDS             2309..2815
                     /locus_tag="G5577_RS24270"
                     /old_locus_tag="G5577_24275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933225.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_164474473.1"
                     /translation="MVNESSRRLTNFTTVLLLLIKECRLERGVHQAQLAEKINKSPSSF
                     AKIETGKSPLTMEVFLACCSTLMISPSAVMSTAERYGNLLTNNGWILLNSSLEENDDEL
                     MKESQEYYSSPGYKSRLNMTSALNGPFYYPNGNVDGLAVFLFAISTHFKNQQLEAQNSL
                     LSGWN"
                     /name="helix-turn-helix transcriptional regulator"
     gene            3492..4016
                     /locus_tag="G5577_RS24275"
                     /old_locus_tag="G5577_24280"
     CDS             3492..4016
                     /locus_tag="G5577_RS24275"
                     /old_locus_tag="G5577_24280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063959429.1"
                     /translation="MQKVTDLYPPEIARHNLQNHFAGNTVMLELIQKLNKTSLCTFAAL
                     CDGNVVTTSGYNITADLCVNRASAVVHSLKQKYLPVTAITISTKADVGGAVKQAAFCID
                     EDDLARLKSEPEKMMKECERNLNSQKQTNAQKEMSRLYKEFGEDGILALLRNVSRSNGN
                     GTPPPSGQPAS"
                     /name="hypothetical protein"
     gene            complement(4143..4571)
                     /locus_tag="G5577_RS24280"
                     /old_locus_tag="G5577_24285"
     CDS             complement(4143..4571)
                     /locus_tag="G5577_RS24280"
                     /old_locus_tag="G5577_24285"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063959428.1"
                     /translation="MRVVIDIEESIVHDILNHANENEDGMSFEETVASLLNDAVNSKKT
                     KTLNDEEINQAILQMVSFAIKNRKENKSFKANELYFKALDDSWNRLSPSTRKSLGRRFR
                     TFVNELWDKATEGEFVIEFKNRNINNAAIYEVVKKVDL"
                     /name="hypothetical protein"
     gene            complement(4872..5387)
                     /locus_tag="G5577_RS24285"
                     /old_locus_tag="G5577_24290"
     CDS             complement(4872..5387)
                     /locus_tag="G5577_RS24285"
                     /old_locus_tag="G5577_24290"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_133257823.1"
                     /translation="MAPPSSTLMNDPRVIGAVIAALLAFLAPTIRDFWITNRTKKNLVK
                     ILIVDVNARIKKIDKFLVPFQHAINKAKLEDTYMPWVSYNEGLEDHIDWKDEKWLMPKD
                     LVSEIVGFYSNTRSLIKFIETINSDRYKEISKDRQIAMLEGLLGDLKESYMEGFTLLQL
                     LQAKQGGR"
                     /name="hypothetical protein"
     gene            complement(5605..5736)
                     /locus_tag="G5577_RS24290"
                     /old_locus_tag="G5577_24295"
     CDS             complement(5605..5736)
                     /locus_tag="G5577_RS24290"
                     /old_locus_tag="G5577_24295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_074172424.1"
                     /translation="MHEPPVQSDCCACSGNYQLESNPERHDKAPLAAATGKQDVREM"
                     /name="replication initiation protein"
     Typing          121..294
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          complement(5590..5976)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.909"
                     /coverage="87.0"
                     /evalue="1.16e-164"
     Typing          722..2223
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="80.392"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttcgccgagg ccttgtcgtt tccctgttaa gcttcttcct ggcatctacc aggaaatttc
       61 cgccccgttc ataagcccgc accgctcgcc gcagccgaac gaccgagcgt agcgagtcag
      121 tgagcgagga agcggaatat ctcctgtatc gcatattctg ctgacgcgct agtgccacct
      181 tttttctcct tccacatgaa gcacttcact gacatcctca tccgtgccaa catattaagc
      241 cagtatacac tccgctagcg ctgcgtgact ggttcagggc tgcgccccga aacccgctaa
      301 aagccactga cgcgcctgcg gcttgtccaa ccccggccga ccgggaaagg ttttcccgcc
      361 cgtcccggcg ttatcaggag accggtttgt cagagaaacg gaacaagatg ctcaccatgt
      421 gggtgacgga ggacgagcac cggcggctgc tcgagcgctg cgacggcagg cagctggccg
      481 cgtggatgcg gcagacctgc ctggacgaaa agcctgcgcg taccggaaag cttccctcac
      541 tctcgccggc gctgctgcgc cagcttgccg gcatgggcaa caacctgaac cagatagccc
      601 gccgcgttaa tgccggcggc ggtacgggcc atgacagggt gcagattgtg gcggcgctga
      661 tggccattga tgccggactt gagcggctgc ggcacgccgt tcttgagaaa ggaacggacg
      721 atgatcgtta aatttcatcc gcgcggccgc ggcggtggcg ccgggcctgt ggattatctg
      781 ctgggtaaag accggcagcg tgacggtgcc accgtcctgc aggggaagcc ggaagaagtc
      841 cgggagctga ttgacgcatc gccttacgtg aagaaataca cctccggcgt cctgtcgttt
      901 gcggaggccg atctgcctcc cggccagcgg gaaaagctga tggccagctt cgagcgggtt
      961 ctgatgcccg gactcgataa agatcagtac agcattctgt gggtggagca cgcggacaag
     1021 gggcgtctgg agctgaactt cctgatcccc aataccgagc tgctgactgg caggcgactc
     1081 cagccatact acgaccgcgc cgaccggccg cgcatcgatg cctggcagac catcgtgaac
     1141 ggcagactgg ggctgcacga cccgaacgcg ccggaaaacc gcagggcgct ggtcacgccg
     1201 tccggcctgc cgaaaacgaa gcaggaggcc gcagaagcca ttacccgggg gctgctgagc
     1261 ttagcctcgt ccggggagct gaaaagccgc caggacgtca ctgacactct tgaaggtgca
     1321 ggttttgagg tggtgcgcac cacgaaaagc agtatcagca tcgccgaccc ggacgggggg
     1381 cgaaacatcc gactgaaggg agccatctat gagcaatctt tttacggcct cgagggactt
     1441 cgaggagaaa ttgaaagcgc agcagcagag taccggcgag atgctgaaag ccgcattcag
     1501 cgagcacgag cagtctgtcg gagcggaact gagcgcaagc gcgaggagaa tcagcgacgc
     1561 catcctcgcc cacgagctga gtatgagcga ggcgatggag aaaaaccggc ggagcgtgct
     1621 gctgacggca gggaggacat ggctcaccat cctgatggtt tcagcgctgc tgatcggcgc
     1681 gagcgggagc attctgtggt ggcaggggca gcagataacc gacaattaca cgacccttcg
     1741 ccagcaggag gacgccctgg cgaagatgag cgcgaaaaca tggggtgtga gctaccagag
     1801 cgacagcaac ggacggttcc tggtgctgcc gaagggagcg aaagccgaaa caggctggac
     1861 agtggacagc gggaagcgga acgcggtgaa gctggtacag gagtagagca gaatgacaga
     1921 gctggaaaag cagttgctga gcgcattaga gcagctacag caggactact cgcaaaggct
     1981 ggacgaatgg gagagcgcct tcgaggaatg gcggaggatg tctgggctta tgcaacggga
     2041 gaacgcgatg ctgaacgagc gcgtatcggg cttgagcagg caggtgcaga gtttgagcga
     2101 gcagctgcgc cgcttgtccg ggagctgaat gccattcagc agaacaaggc gcaggaaaag
     2161 ctgcatatga aagatatgac agctgagcgg caatctaaaa aaacatacgg tgggccaacg
     2221 ttaggataat tttcaatttc ctgttgtgta atatcgtttc ctgttatatt gtttttgaga
     2281 acttaaaatt cacctcagga aatttgaaat ggtcaatgaa tcatctagaa ggcttactaa
     2341 ttttacaact gtactacttc ttcttatcaa agaatgtcga ctcgagaggg gagttcatca
     2401 ggctcaacta gctgaaaaga taaataaaag cccgagttca tttgcaaaaa tcgagactgg
     2461 taagagccct cttacgatgg aggtctttct tgcatgttgc agtacgttga tgatttcacc
     2521 atcagctgta atgtctactg cggagcgtta tggaaacctg ttaaccaata atggttggat
     2581 cttgcttaac agttctctgg aagagaatga tgatgaactt atgaaagaat cccaggagta
     2641 ctattcatca cctggttata aaagcaggct aaatatgact tcagctctta atgggccgtt
     2701 ctattatcca aatggtaatg ttgatgggtt agctgttttc ctttttgcta tatctaccca
     2761 ctttaaaaat caacagcttg aagctcaaaa ctctctttta tctggatgga attaaagtcc
     2821 gataaataga gtgtatggag ttaattttcc ctaagatggc tccacataca catttcagca
     2881 tagttatgca ttcggttaaa atttacctgt cgcgatcggg ggggtttctc gggtggtttg
     2941 ttacccgttt tacctgatag ccgccccgtt cgcggcgaac ctgtctggag gggttcgggc
     3001 aacggatgtt atggtaaatg tcgagatttt gagttgttca gctgacgatt ttcgcatcaa
     3061 atttttgctg tatgtttaaa catgatttta acatcaatgt catctgaggg tgttgttgct
     3121 aatttcatct acctgacagg taatttcctt ttaaatcaag agcgaaagat cagcgaaaaa
     3181 gctatgtttg cgtttgtgga atagggtaga ataggggatt ctgatgatcg tgaggttgtc
     3241 agtaaaaacg aggcagctat cagatcttcg gcctgagaaa ctaaggattc tgatagctgc
     3301 cggtgactca gttcgcgaaa actgaatctt acctgatcaa caaacgcccg ataagggcgt
     3361 ttgtcaagtg gtgctgcatt atgtagtaac ttggtaccta cttgccaggc atttttcaca
     3421 gttttttgat gtgcgaaggc tcatccggac accgtcacct gttatctaat ccttataaac
     3481 aggtgctata aatgcaaaaa gtaactgatc tgtatccccc tgaaattgct cgtcataatc
     3541 ttcaaaatca cttcgctggc aatactgtta tgctggagtt aatccagaaa ctaaataaaa
     3601 ccagcttatg tacttttgcg gcgctctgtg acggcaacgt cgtgaccact tccggctata
     3661 acattacggc cgatctgtgt gttaaccgtg cgtcagccgt ggtccattcg cttaagcaaa
     3721 aatatctgcc ggttacggcc ataacgattt cgactaaggc cgatgtaggt ggtgcggtaa
     3781 agcaggcagc tttctgtatc gatgaggacg atcttgcaag gctgaaatca gagccggaaa
     3841 agatgatgaa agagtgcgaa cgtaacctga acagccagaa gcagaccaat gctcagaagg
     3901 aaatgagcag gttgtacaag gagtttggtg aagatggaat tctggctcta ttgcgcaacg
     3961 tttcccgttc gaacgggaat ggcacgccgc cgccaagcgg gcagcctgcg agctgatgat
     4021 ccttagctaa ctggaaatac taaagcccgc tttgcgggct ttttcatacg tttaatacaa
     4081 aggaataatt atttctcaga ctttacaaaa aaactattaa ggccgctttt gtaattcact
     4141 gcctacagat caaccttttt cacgacctca tatattgctg cgttattgat gttgcgattt
     4201 ttgaattcaa ttacgaactc accttctgtt gctttgtccc acagttcgtt tacaaatgtg
     4261 cgaaagcgac gacctaaaga cttcctggtt gatgggctaa gcctgttcca agaatcatct
     4321 aacgctttga aatataattc atttgctttg aagcttttgt tttcttttct attttttatt
     4381 gcaaaagaaa ccatttgaag tattgcttgg ttaatttctt catcgtttag cgttttggtt
     4441 tttttgctgt taactgcgtc attcagcaat gatgcgaccg tttcttcaaa actcatccca
     4501 tcttcgtttt catttgcatg gttaagaatg tcatgcacga tagactcttc tatatcaata
     4561 acaactctca tataaacccc ttgtttttgg ttcttttgtg ttgtttttaa atatagcgcc
     4621 aaaaattaaa catgtaaaca tgtttcttgt tgtttcttgt tgtttgtggt tgttttctct
     4681 ttgtggggcg tcaggggagt tgaaggcttg aagaggtcga agatacttgt tacaaaatcg
     4741 ccctaatgca gattaggcat tagagcgctt ttctttgaca ttgtgagttg gttcgtcaac
     4801 aatcgacagt aggaatctaa tttacagagg tgtgatatgt ataaagatat tggaccaggc
     4861 ccaatcgtat tctaacgacc accttgcttt gcttggagga gttgcagcaa tgtgaacccc
     4921 tccatgtagc tttcttttaa gtctcccagc aacccctcaa gcatagctat ttgtctgtcc
     4981 ttagatattt ccttgtatct gtctgaattg atggtttcta taaacttgat aagagaccga
     5041 gtattgctat aaaaacctac gatttcagaa actaaatctt tcggcattag ccatttctca
     5101 tctttccaat caatgtgatc ttcaaggcct tcgttataac taacccacgg catatacgta
     5161 tcttcaagtt tggctttgtt gatagcgtgt tgaaatggaa caagaaattt atctattttc
     5221 tttatgcgtg cgttaacgtc cacaatgaga atcttaacga ggtttttctt tgtcctattg
     5281 gtaatccaaa aatctctgat tgttggagct agaaatgcca aaagggctgc tatcactgcg
     5341 cctatgactc tgggatcatt cattaaagtt gagcttggcg gagccattcc aggagatact
     5401 ccatgtaagg gaagagtaag aattgacgat aagttattat aaagcgtaag ctaacaaacg
     5461 aaaaaaccac ccggcaaggt ggtttttcga aggtccagca aaccggcaag tttctgaacc
     5521 ttggtaacag tcttgtgcga gacgtgtcac caaatctgtc cttccagtgt agcctcactc
     5581 aggccactac ttcaagaact cttgctacat ctctcgcaca tcctgtttac cagtggctgc
     5641 tgccagtggc gctttgtcgt gtctttccgg gttggactca agttgatagt taccggaaca
     5701 ggcgcagcag tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc
     5761 cttcccggaa ccgagtgtca ggcgtggaat gagaaaccgc ggccataaca gcggagtgac
     5821 accggcaacc gaaaggcagg aacaggagag cgcacgaggg agccaccagg gggaaacgcc
     5881 tggtatcttt atagtcctgt cgggtttcgc caccactgat ttgagcgtca gattctgtga
     5941 tgcttgtcag gggggcggag cctatggaaa aacggc
//
