LOCUS       NZ_CP049191             2495 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain Y323 plasmid pY323-3, complete
            sequence.
ACCESSION   NZ_CP049191
VERSION     NZ_CP049191.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14142412
            Assembly: GCF_011045335.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Zhu,X.
  TITLE     Prevalence of Aminoglycoside Resistance Genes and Molecular
            Characterization of a Novel Gene aac(3)-IIg among Clinical Isolates
            of Enterobacter cloacae Complex from a Chinese Teaching Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Zhu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-FEB-2020) Laboratory Medicine, College of Life
            Science, Wenzhou Medical University, Wenzhou Medical University,
            Wenzhou, Zhejiang 325000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011045335.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:54:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,005
            CDSs (total)                      :: 4,891
            Genes (coding)                    :: 4,712
            CDSs (with protein)               :: 4,712
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 95 of 179
            Pseudo Genes (incomplete)         :: 91 of 179
            Pseudo Genes (internal stop)      :: 35 of 179
            Pseudo Genes (multiple problems)  :: 38 of 179
            Pseudo Genes (short protein)      :: 1 of 179
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049191.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Y323"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pY323-3"
                     /geo_loc_name="China:wenzhou"
                     /collection_date="2007-01-31"
     gene            481..699
                     /locus_tag="G5C52_RS24880"
                     /old_locus_tag="G5C52_24835"
     CDS             481..699
                     /locus_tag="G5C52_RS24880"
                     /old_locus_tag="G5C52_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(990..1175)
                     /locus_tag="G5C52_RS24885"
                     /old_locus_tag="G5C52_24840"
     CDS             complement(990..1175)
                     /locus_tag="G5C52_RS24885"
                     /old_locus_tag="G5C52_24840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1541..1768)
                     /locus_tag="G5C52_RS24890"
                     /old_locus_tag="G5C52_24845"
     CDS             complement(1541..1768)
                     /locus_tag="G5C52_RS24890"
                     /old_locus_tag="G5C52_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          856..943
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(2..229)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.565"
                     /coverage="97.0"
                     /evalue="8.86e-76"
     Typing          complement(2004..2486)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.96"
                     /coverage="95.0"
                     /evalue="2.29e-126"
ORIGIN
        1 gggtttcgcc accactgatt tgagcgtcag attctgtgat gcttgtcagg ggggcggagc
       61 ctatggaaaa acggctttgg cgcggcctta tgctttcttc gttaagtatc ttcctggcat
      121 cccccaggaa atttctgatc catccgtaag cccgtcccgc tcgccgcagc cgaacgaccg
      181 agcggagcga gtcagtgagc gaggaagcgg aatatattct gtatcacatt ttctcctgac
      241 gcgttttctt tcactttctg cgcctgtctt atgtggcatt aatgctatgt gttactgcca
      301 tgctacatct taagccagta tacactccgc tagtgctccg tgactggtcc ggcgctgcgc
      361 ccggaacccg cctgtaccgg ttcagcagcc gttccggcct gactgcaatt tttttttttt
      421 catccctgcc cgctaccctg taaacctttc ttctgcgttg ccgttaacag taggtaagcc
      481 atgtttggtt tttcttccgg agccctgatg actcttagtg atgatgagcg acacctgctt
      541 gtgtccgttg tttcggtctg gctacggcgt gccggagggg atgccggagc catgatgctg
      601 gatgcatacc ggcagatttt gtctgaaacc gaaccggccg taaggactgt catgctcgaa
      661 tttcttgaat ccgttcgcat tcattacatt tcgtcctgag cattttctgt atttctgtcc
      721 atacttaaac gaggcggccg tcacggacgg tggtcgtcgt tccatgatgc tgttatggac
      781 gagtgagagt aatctaatct aacttgattg agttccgggc gtgccgcccg gttttttttg
      841 ctgtttcccg gtgtccgttc cgggacagga tgtgcatttg taataccgcg cacgcgccgg
      901 catcacaaat accctggtca gggctgcgcc ccgacacccc gccatcgcct cacggccgcg
      961 cactgaattg cgcacccgtt caccgatttt cacgcatttt cctcgcctat ctgtgcgttc
     1021 agcatggcgt aaagcgcttc ggcctgatcg tgcaggtgct cacagcagcc ggcagcctca
     1081 tccagatcca tctgctcgag ccgctcaagg aggagaagtg tctgtgcgcg gataaatttg
     1141 gccatattga gggcggcgga ttcctgtctg gtcactggtg attttccccg tctggtgatg
     1201 atttttgcgt gaaaagagca tggcgtcatt ctggtgcata gtcatgcatg ccgttaaaat
     1261 ttaacaggcg cgtttctgac gggttccggg gtggtttgtt gtggtttttg tcatggtttc
     1321 gtcaggaacg cgctgaacgc gtctcaggcg gtgcgggcaa cggatgttat ggtaaattct
     1381 tgggggcttg attataaaat accgcacttc ctcttatgtc ttgtgctttt tagacaaccc
     1441 tgaaagcttt tagctttggt gatgcaattt gctgggattt tggcggagaa ccacaggtaa
     1501 agaaaaaggc cacattagcg gcctttttcg gagaagatgc tcagtgcgga atttcggaga
     1561 cttctttaac gtcggtttta ttgatctgct gtttgacacc attcgcgtct gtatagccaa
     1621 gcaggcctga gtctgattct ttaggtttgc cgtcgctgac aatagtgcga ccgtcattgg
     1681 tgtgtatggc atagcttgtg cgcgtgcagc cggtgagggc tgaaagcgca actgccgctg
     1741 cgaacactga aacaaaaatc tttttcaaag ccagctcctt ttatccatat cagttttggt
     1801 cactaaccat ctaactatag gtcatttttt atgtgaaaag aggcttgatg gtgggctgta
     1861 tatcgagtta ggcgcgaggt gctatggtca aagtgtggtg tcaggcgggg aggaggctta
     1921 tatgagatgt aatccggccg atacatatac caagatgatt taagtattcc tctttgtgag
     1981 gttggatgtc taatttataa aggatcttct tgagatcctt tttttccgcg cgtaatctct
     2041 tgccctgtaa acgaaaaaac caccctggca ggtggttttt cgaaggttag gtaatcctgg
     2101 cagatcccct aaccgtggta acagtcttgt gcgagacatg tcaccaaatt tgtcctttca
     2161 gtgtagcctc actaaggccg ccacttcaag aactcttgag acatctctcg cacatcctgt
     2221 ttgccaatgg ccgttgccaa tggcgattag tcgtgtcttt ccgggttgga ctcaagttga
     2281 tagttaccgg ataaggcgca gcggtcggac tgaacggggg gttcgtgcat acagtccatc
     2341 ctggagcgaa ctgccttccc ggaactgagt gtcaggcgtg gaatgagaaa ccgcggccat
     2401 aacagcggag tgacaccggt aaaccgaaag gcaggaatgc gggggagcac gagggagcca
     2461 ccagggggaa acgcctggta tctttaagcc gcatc
//
