LOCUS       NZ_CP049190             5976 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain Y323 plasmid pY323-2, complete
            sequence.
ACCESSION   NZ_CP049190
VERSION     NZ_CP049190.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14142412
            Assembly: GCF_011045335.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Zhu,X.
  TITLE     Prevalence of Aminoglycoside Resistance Genes and Molecular
            Characterization of a Novel Gene aac(3)-IIg among Clinical Isolates
            of Enterobacter cloacae Complex from a Chinese Teaching Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   Zhu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-FEB-2020) Laboratory Medicine, College of Life
            Science, Wenzhou Medical University, Wenzhou Medical University,
            Wenzhou, Zhejiang 325000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011045335.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:54:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,005
            CDSs (total)                      :: 4,891
            Genes (coding)                    :: 4,712
            CDSs (with protein)               :: 4,712
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 95 of 179
            Pseudo Genes (incomplete)         :: 91 of 179
            Pseudo Genes (internal stop)      :: 35 of 179
            Pseudo Genes (multiple problems)  :: 38 of 179
            Pseudo Genes (short protein)      :: 1 of 179
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049190.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Y323"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pY323-2"
                     /geo_loc_name="China:wenzhou"
                     /collection_date="2007-01-31"
     gene            483..806
                     /locus_tag="G5C52_RS24835"
                     /old_locus_tag="G5C52_24790"
     CDS             483..806
                     /locus_tag="G5C52_RS24835"
                     /old_locus_tag="G5C52_24790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_029593203.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1015..>1455
                     /locus_tag="G5C52_RS25290"
                     /pseudo=""
     CDS             1015..>1455
                     /locus_tag="G5C52_RS25290"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1484..1981
                     /locus_tag="G5C52_RS24845"
                     /old_locus_tag="G5C52_24800"
     CDS             1484..1981
                     /locus_tag="G5C52_RS24845"
                     /old_locus_tag="G5C52_24800"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_164474471.1"
                     /translation="MSNLFTASRDFEEKLKAQQQSTGEMLKAAFSEHEQSVGAELSASA
                     RRISDAILAHELSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIGASGSILWWQGQQIT
                     DNYTTLRQQEDALAKMSAKTWGVSYQSDSNGRFLVLPKGAKAETGWTVDSGKRNAVKLV
                     QE"
                     /name="MbeB family mobilization protein"
     gene            1988..2203
                     /locus_tag="G5C52_RS24850"
                     /old_locus_tag="G5C52_24805"
     CDS             1988..2203
                     /locus_tag="G5C52_RS24850"
                     /old_locus_tag="G5C52_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_164474472.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFEEWRRMSGLMQRENA
                     MLNERVSGLSRQVQSLSEQLRRLSGS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2384..2890
                     /locus_tag="G5C52_RS24855"
                     /old_locus_tag="G5C52_24810"
     CDS             2384..2890
                     /locus_tag="G5C52_RS24855"
                     /old_locus_tag="G5C52_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933225.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_164474473.1"
                     /translation="MVNESSRRLTNFTTVLLLLIKECRLERGVHQAQLAEKINKSPSSF
                     AKIETGKSPLTMEVFLACCSTLMISPSAVMSTAERYGNLLTNNGWILLNSSLEENDDEL
                     MKESQEYYSSPGYKSRLNMTSALNGPFYYPNGNVDGLAVFLFAISTHFKNQQLEAQNSL
                     LSGWN"
                     /name="helix-turn-helix transcriptional regulator"
     gene            3567..4091
                     /locus_tag="G5C52_RS24860"
                     /old_locus_tag="G5C52_24815"
     CDS             3567..4091
                     /locus_tag="G5C52_RS24860"
                     /old_locus_tag="G5C52_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063959429.1"
                     /translation="MQKVTDLYPPEIARHNLQNHFAGNTVMLELIQKLNKTSLCTFAAL
                     CDGNVVTTSGYNITADLCVNRASAVVHSLKQKYLPVTAITISTKADVGGAVKQAAFCID
                     EDDLARLKSEPEKMMKECERNLNSQKQTNAQKEMSRLYKEFGEDGILALLRNVSRSNGN
                     GTPPPSGQPAS"
                     /name="hypothetical protein"
     gene            complement(4218..4646)
                     /locus_tag="G5C52_RS24865"
                     /old_locus_tag="G5C52_24820"
     CDS             complement(4218..4646)
                     /locus_tag="G5C52_RS24865"
                     /old_locus_tag="G5C52_24820"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063959428.1"
                     /translation="MRVVIDIEESIVHDILNHANENEDGMSFEETVASLLNDAVNSKKT
                     KTLNDEEINQAILQMVSFAIKNRKENKSFKANELYFKALDDSWNRLSPSTRKSLGRRFR
                     TFVNELWDKATEGEFVIEFKNRNINNAAIYEVVKKVDL"
                     /name="hypothetical protein"
     gene            complement(4947..5462)
                     /locus_tag="G5C52_RS24870"
                     /old_locus_tag="G5C52_24825"
     CDS             complement(4947..5462)
                     /locus_tag="G5C52_RS24870"
                     /old_locus_tag="G5C52_24825"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_133257823.1"
                     /translation="MAPPSSTLMNDPRVIGAVIAALLAFLAPTIRDFWITNRTKKNLVK
                     ILIVDVNARIKKIDKFLVPFQHAINKAKLEDTYMPWVSYNEGLEDHIDWKDEKWLMPKD
                     LVSEIVGFYSNTRSLIKFIETINSDRYKEISKDRQIAMLEGLLGDLKESYMEGFTLLQL
                     LQAKQGGR"
                     /name="hypothetical protein"
     gene            complement(5680..5811)
                     /locus_tag="G5C52_RS24875"
                     /old_locus_tag="G5C52_24830"
     CDS             complement(5680..5811)
                     /locus_tag="G5C52_RS24875"
                     /old_locus_tag="G5C52_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_074172424.1"
                     /translation="MHEPPVQSDCCACSGNYQLESNPERHDKAPLAAATGKQDVREM"
                     /name="replication initiation protein"
     Typing          196..369
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          complement(2..229)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.174"
                     /coverage="99.0"
                     /evalue="8.73e-86"
     Typing          complement(5532..5976)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.602"
                     /coverage="88.0"
                     /evalue="1.71e-152"
     Typing          797..2298
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="80.392"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gggtttcgcc accactgatt tgagcgtcag attctgtgat gcttgtcagg ggggcggagc
       61 ctatggaaaa acggcttcgc cgaggccttg tcgtttccct gttaagcttc ttcctggcat
      121 ctaccaggaa atttccgccc cgttcataag cccgcaccgc tcgccgcagc cgaacgaccg
      181 agcgtagcga gtcagtgagc gaggaagcgg aatatctcct gtatcgcata ttctgctgac
      241 gcgctagtgc cacctttttt ctccttccac atgaagcact tcactgacat cctcatccgt
      301 gccaacatat taagccagta tacactccgc tagcgctgcg tgactggttc agggctgcgc
      361 cccgaaaccc gctaaaagcc actgacgcgc ctgcggcttg tccaaccccg gccgaccggg
      421 aaaggttttc ccgcccgtcc cggcgttatc aggagaccgg tttgtcagag aaacggaaca
      481 agatgctcac catgtgggtg acggaggacg agcaccggcg gctgctcgag cgctgcgacg
      541 gcaggcagct ggccgcgtgg atgcggcaga cctgcctgga cgaaaagcct gcgcgtaccg
      601 gaaagcttcc ctcactctcg ccggcgctgc tgcgccagct tgccggcatg ggcaacaacc
      661 tgaaccagat agcccgccgc gttaatgccg gcggcggtac gggccatgac agggtgcaga
      721 ttgtggcggc gctgatggcc attgatgccg gacttgagcg gctgcggcac gccgttcttg
      781 agaaaggaac ggacgatgat cgttaaattt catccgcgcg gccgcggcgg tggcgccggg
      841 cctgtggatt atctgctggg taaagaccgg cagcgtgacg gtgccaccgt cctgcagggg
      901 aagccggaag aagtccggga gctgattgac gcatcgcctt acgtgaagaa atacacctcc
      961 ggcgtcctgt cgtttgcgga ggccgatctg cctcccggcc agcgggaaaa gctgatggcc
     1021 agcttcgagc gggttctgat gcccggactc gataaagatc agtacagcat tctgtgggtg
     1081 gagcacgcgg acaaggggcg tctggagctg aacttcctga tccccaatac cgagctgctg
     1141 actggcaggc gactccagcc atactacgac cgcgccgacc ggccgcgcat cgatgcctgg
     1201 cagaccatcg tgaacggcag actggggctg cacgacccga acgcgccgga aaaccgcagg
     1261 gcgctggtca cgccgtccgg cctgccgaaa acgaagcagg aggccgcaga agccattacc
     1321 cgggggctgc tgagcttagc ctcgtccggg gagctgaaaa gccgccagga cgtcactgac
     1381 actcttgaag gtgcaggttt tgaggtggtg cgcaccacga aaagcagtat cagcatcgcc
     1441 gacccggacg gggggcgaaa catccgactg aagggagcca tctatgagca atctttttac
     1501 ggcctcgagg gacttcgagg agaaattgaa agcgcagcag cagagtaccg gcgagatgct
     1561 gaaagccgca ttcagcgagc acgagcagtc tgtcggagcg gaactgagcg caagcgcgag
     1621 gagaatcagc gacgccatcc tcgcccacga gctgagtatg agcgaggcga tggagaaaaa
     1681 ccggcggagc gtgctgctga cggcagggag gacatggctc accatcctga tggtttcagc
     1741 gctgctgatc ggcgcgagcg ggagcattct gtggtggcag gggcagcaga taaccgacaa
     1801 ttacacgacc cttcgccagc aggaggacgc cctggcgaag atgagcgcga aaacatgggg
     1861 tgtgagctac cagagcgaca gcaacggacg gttcctggtg ctgccgaagg gagcgaaagc
     1921 cgaaacaggc tggacagtgg acagcgggaa gcggaacgcg gtgaagctgg tacaggagta
     1981 gagcagaatg acagagctgg aaaagcagtt gctgagcgca ttagagcagc tacagcagga
     2041 ctactcgcaa aggctggacg aatgggagag cgccttcgag gaatggcgga ggatgtctgg
     2101 gcttatgcaa cgggagaacg cgatgctgaa cgagcgcgta tcgggcttga gcaggcaggt
     2161 gcagagtttg agcgagcagc tgcgccgctt gtccgggagc tgaatgccat tcagcagaac
     2221 aaggcgcagg aaaagctgca tatgaaagat atgacagctg agcggcaatc taaaaaaaca
     2281 tacggtgggc caacgttagg ataattttca atttcctgtt gtgtaatatc gtttcctgtt
     2341 atattgtttt tgagaactta aaattcacct caggaaattt gaaatggtca atgaatcatc
     2401 tagaaggctt actaatttta caactgtact acttcttctt atcaaagaat gtcgactcga
     2461 gaggggagtt catcaggctc aactagctga aaagataaat aaaagcccga gttcatttgc
     2521 aaaaatcgag actggtaaga gccctcttac gatggaggtc tttcttgcat gttgcagtac
     2581 gttgatgatt tcaccatcag ctgtaatgtc tactgcggag cgttatggaa acctgttaac
     2641 caataatggt tggatcttgc ttaacagttc tctggaagag aatgatgatg aacttatgaa
     2701 agaatcccag gagtactatt catcacctgg ttataaaagc aggctaaata tgacttcagc
     2761 tcttaatggg ccgttctatt atccaaatgg taatgttgat gggttagctg ttttcctttt
     2821 tgctatatct acccacttta aaaatcaaca gcttgaagct caaaactctc ttttatctgg
     2881 atggaattaa agtccgataa atagagtgta tggagttaat tttccctaag atggctccac
     2941 atacacattt cagcatagtt atgcattcgg ttaaaattta cctgtcgcga tcgggggggt
     3001 ttctcgggtg gtttgttacc cgttttacct gatagccgcc ccgttcgcgg cgaacctgtc
     3061 tggaggggtt cgggcaacgg atgttatggt aaatgtcgag attttgagtt gttcagctga
     3121 cgattttcgc atcaaatttt tgctgtatgt ttaaacatga ttttaacatc aatgtcatct
     3181 gagggtgttg ttgctaattt catctacctg acaggtaatt tccttttaaa tcaagagcga
     3241 aagatcagcg aaaaagctat gtttgcgttt gtggaatagg gtagaatagg ggattctgat
     3301 gatcgtgagg ttgtcagtaa aaacgaggca gctatcagat cttcggcctg agaaactaag
     3361 gattctgata gctgccggtg actcagttcg cgaaaactga atcttacctg atcaacaaac
     3421 gcccgataag ggcgtttgtc aagtggtgct gcattatgta gtaacttggt acctacttgc
     3481 caggcatttt tcacagtttt ttgatgtgcg aaggctcatc cggacaccgt cacctgttat
     3541 ctaatcctta taaacaggtg ctataaatgc aaaaagtaac tgatctgtat ccccctgaaa
     3601 ttgctcgtca taatcttcaa aatcacttcg ctggcaatac tgttatgctg gagttaatcc
     3661 agaaactaaa taaaaccagc ttatgtactt ttgcggcgct ctgtgacggc aacgtcgtga
     3721 ccacttccgg ctataacatt acggccgatc tgtgtgttaa ccgtgcgtca gccgtggtcc
     3781 attcgcttaa gcaaaaatat ctgccggtta cggccataac gatttcgact aaggccgatg
     3841 taggtggtgc ggtaaagcag gcagctttct gtatcgatga ggacgatctt gcaaggctga
     3901 aatcagagcc ggaaaagatg atgaaagagt gcgaacgtaa cctgaacagc cagaagcaga
     3961 ccaatgctca gaaggaaatg agcaggttgt acaaggagtt tggtgaagat ggaattctgg
     4021 ctctattgcg caacgtttcc cgttcgaacg ggaatggcac gccgccgcca agcgggcagc
     4081 ctgcgagctg atgatcctta gctaactgga aatactaaag cccgctttgc gggctttttc
     4141 atacgtttaa tacaaaggaa taattatttc tcagacttta caaaaaaact attaaggccg
     4201 cttttgtaat tcactgccta cagatcaacc tttttcacga cctcatatat tgctgcgtta
     4261 ttgatgttgc gatttttgaa ttcaattacg aactcacctt ctgttgcttt gtcccacagt
     4321 tcgtttacaa atgtgcgaaa gcgacgacct aaagacttcc tggttgatgg gctaagcctg
     4381 ttccaagaat catctaacgc tttgaaatat aattcatttg ctttgaagct tttgttttct
     4441 tttctatttt ttattgcaaa agaaaccatt tgaagtattg cttggttaat ttcttcatcg
     4501 tttagcgttt tggttttttt gctgttaact gcgtcattca gcaatgatgc gaccgtttct
     4561 tcaaaactca tcccatcttc gttttcattt gcatggttaa gaatgtcatg cacgatagac
     4621 tcttctatat caataacaac tctcatataa accccttgtt tttggttctt ttgtgttgtt
     4681 tttaaatata gcgccaaaaa ttaaacatgt aaacatgttt cttgttgttt cttgttgttt
     4741 gtggttgttt tctctttgtg gggcgtcagg ggagttgaag gcttgaagag gtcgaagata
     4801 cttgttacaa aatcgcccta atgcagatta ggcattagag cgcttttctt tgacattgtg
     4861 agttggttcg tcaacaatcg acagtaggaa tctaatttac agaggtgtga tatgtataaa
     4921 gatattggac caggcccaat cgtattctaa cgaccacctt gctttgcttg gaggagttgc
     4981 agcaatgtga acccctccat gtagctttct tttaagtctc ccagcaaccc ctcaagcata
     5041 gctatttgtc tgtccttaga tatttccttg tatctgtctg aattgatggt ttctataaac
     5101 ttgataagag accgagtatt gctataaaaa cctacgattt cagaaactaa atctttcggc
     5161 attagccatt tctcatcttt ccaatcaatg tgatcttcaa ggccttcgtt ataactaacc
     5221 cacggcatat acgtatcttc aagtttggct ttgttgatag cgtgttgaaa tggaacaaga
     5281 aatttatcta ttttctttat gcgtgcgtta acgtccacaa tgagaatctt aacgaggttt
     5341 ttctttgtcc tattggtaat ccaaaaatct ctgattgttg gagctagaaa tgccaaaagg
     5401 gctgctatca ctgcgcctat gactctggga tcattcatta aagttgagct tggcggagcc
     5461 attccaggag atactccatg taagggaaga gtaagaattg acgataagtt attataaagc
     5521 gtaagctaac aaacgaaaaa accacccggc aaggtggttt ttcgaaggtc cagcaaaccg
     5581 gcaagtttct gaaccttggt aacagtcttg tgcgagacgt gtcaccaaat ctgtccttcc
     5641 agtgtagcct cactcaggcc actacttcaa gaactcttgc tacatctctc gcacatcctg
     5701 tttaccagtg gctgctgcca gtggcgcttt gtcgtgtctt tccgggttgg actcaagttg
     5761 atagttaccg gaacaggcgc agcagtcgga ctgaacgggg ggttcgtgca tacagtccag
     5821 cttggagcga actgccttcc cggaaccgag tgtcaggcgt ggaatgagaa accgcggcca
     5881 taacagcgga gtgacaccgg caaccgaaag gcaggaacag gagagcgcac gagggagcca
     5941 ccagggggaa acgcctggta tctttatagt cctgtc
//
