LOCUS       NZ_CP049131             2100 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli strain EC931 plasmid pEC931_11, complete sequence.
ACCESSION   NZ_CP049131
VERSION     NZ_CP049131.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14164122
            Assembly: GCF_014522225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of a tigecycline and colistin resistant
            Escherichia coli isolate EC931
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. 3.12; Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/15/2023 02:18:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,104
            CDSs (total)                      :: 4,985
            Genes (coding)                    :: 4,684
            CDSs (with protein)               :: 4,684
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 83 of 301
            Pseudo Genes (incomplete)         :: 207 of 301
            Pseudo Genes (internal stop)      :: 51 of 301
            Pseudo Genes (multiple problems)  :: 40 of 301
            Pseudo Genes (short protein)      :: 5 of 301
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049131.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC931"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC931_11"
                     /geo_loc_name="China: Yiwu"
                     /lat_lon="29.334975 N 120.121983 E"
                     /collection_date="2017-02-10"
                     /collected_by="Jianyong Wu"
     gene            complement(40..306)
                     /locus_tag="G6G93_RS25185"
                     /old_locus_tag="G6G93_25280"
     CDS             complement(40..306)
                     /locus_tag="G6G93_RS25185"
                     /old_locus_tag="G6G93_25280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(310..1275)
                     /locus_tag="G6G93_RS25190"
                     /old_locus_tag="G6G93_25285"
     CDS             complement(310..1275)
                     /locus_tag="G6G93_RS25190"
                     /old_locus_tag="G6G93_25285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(651..882)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 tacctatcac ctcagcctgc cgctggctct tttccagaac taagaatgcg ttcttaaaaa
       61 tccaaacttg ataccgttta aatctatttt cacaaaaaga gtttttaccc tctcgtttgt
      121 tttctgaata tataaaatta attcatcctc aaaaccttcg tgcggatgaa aaccaaatgt
      181 gccaaaactt aatccatcaa taagcaaaat aaggttttta aacctgtcag gaatcaccgc
      241 ttcacttctt aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat
      301 attcatacgt taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga
      361 atcccagaca aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat
      421 gacaagatcc tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt
      481 cgagataaaa cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt
      541 tgccatatct tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg
      601 ctcggcaacg ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt
      661 cggctcatag ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac
      721 gtagttcttc cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg
      781 tgcgctgcca tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga
      841 tagctccttt cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc
      901 aagttcacct atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg
      961 gtaatccaca acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct
     1021 tctccactgg cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag
     1081 gcgcgtttct ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga
     1141 ggccatacgg agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga
     1201 atcggtaagc gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa
     1261 tttatcttct gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat
     1321 tcatcacgta cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga
     1381 cacattcgaa atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa
     1441 ttctgctcca ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta
     1501 aaccactatt tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag
     1561 aattgcgcaa aaaataacca caattttaac ccccaaaaca agcacccccg aacggcttac
     1621 cccaaccttt tccacacacc cgatcaccaa aacagggaat aaaaacaaaa ccctgtctta
     1681 caggtggatg gcctcacttc gtgaaaccat ccaccccgca taagtttttt aatcggtggt
     1741 gtcgtcaatc actaaatcgg aaccccaaag gggaccccca cgatttagca tttgacgaga
     1801 ttaactctcg gagatggcat catgctttta acggacttta aaaaacggtg aacggatgcg
     1861 gattttaatc cgtgtctgtg aggcgttgag caacgagcaa agcgaggcgg tagaaagaga
     1921 gccagcggca ggctcaggac gataatccgg ggaaaatata ccactcgcag agtgttaaca
     1981 tttgacatcg gattgtctat cctgcattgg tgtcgagttg gcaaggctga ggataatgct
     2041 gaggataatg ctgaggataa tgcaggatag acagagcact agagattgca tgcaatctcg
//
