LOCUS       NZ_CP049130             2275 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli strain EC931 plasmid pEC931_10, complete sequence.
ACCESSION   NZ_CP049130
VERSION     NZ_CP049130.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14164122
            Assembly: GCF_014522225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of a tigecycline and colistin resistant
            Escherichia coli isolate EC931
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. 3.12; Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/15/2023 02:18:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,104
            CDSs (total)                      :: 4,985
            Genes (coding)                    :: 4,684
            CDSs (with protein)               :: 4,684
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 83 of 301
            Pseudo Genes (incomplete)         :: 207 of 301
            Pseudo Genes (internal stop)      :: 51 of 301
            Pseudo Genes (multiple problems)  :: 40 of 301
            Pseudo Genes (short protein)      :: 5 of 301
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049130.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC931"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC931_10"
                     /geo_loc_name="China: Yiwu"
                     /lat_lon="29.334975 N 120.121983 E"
                     /collection_date="2017-02-10"
                     /collected_by="Jianyong Wu"
     gene            complement(136..1146)
                     /locus_tag="G6G93_RS25180"
                     /old_locus_tag="G6G93_25275"
     CDS             complement(136..1146)
                     /locus_tag="G6G93_RS25180"
                     /old_locus_tag="G6G93_25275"
                     /inference="COORDINATES: protein motif:HMM:NF013603.2"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001681832.1"
                     /translation="MKNPDSPILSLRDYSTQDSKWDSDRARADQVAKIYASDQAFFRRG
                     ERMFDCSQRLQFAPQSSRLTGEMRLALRHGEFCHVPFCPVCSRRRSLRWMRRLWEALPK
                     LLAEHPTASWLFLTLTVKNPPVGELRETLRQMNAAWERLIKRKAFAPVLGWLRSTEITY
                     GNVRGCCHPHFHVVLWVPSSWFTRDYVKHERWVEIWSECLRVDYAAGAHIKRVRPKRGK
                     VPEGASFAEVQRAALEAGVIETLKYTVKSSEIVRDPAWFLELARQTYGLRMIATGGRFR
                     EILQVEKPETDEDLIGADMPAKPDEFEEMAFWLAFDWRRDEKRYKRNPKADRRKE"
                     /name="protein rep"
     Typing          complement(1728..1810)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.882"
                     /coverage="93.0"
                     /evalue="1.53e-15"
ORIGIN
        1 caaaacgctt ctgagcgcct taaaacgaaa aaacgggcaa atacttgccc gtttctgaaa
       61 atcgcgttag aacgcttctg agcgcgtttc acctccacta ccgcactatt cccacaatgt
      121 gcctctgaaa cacgctcact ccttccgcct gtctgctttt ggattccgct tgtagcgttt
      181 ctcgtcacgt ctccagtcaa acgccagcca gaacgccatc tcctcgaact cgtccggctt
      241 cgctggcata tcagcaccaa tcaaatcctc gtccgtttcc ggcttctcga cctggaggat
      301 ctcccgaaac cgtcctcccg tggcaatcat ccgcagcccg taggtctgcc tcgccagctc
      361 cagaaaccac gccggatcgc gcacgatttc cgatgatttc accgtgtatt tcagcgtctc
      421 gatcacgccc gcttccagcg ctgcccgctg cacctcagca aaacttgccc cttccggcac
      481 tttcccccgt tttggccgca cgcgcttgat gtgcgcgcca gctgcgtaat ccacccgcag
      541 gcattcactc cagatttcca cccagcgctc atgtttcacg taatcccgcg tgaaccatga
      601 cgaaggaacc cagagcacca catggaagtg cgggtgacaa catccgcgca cattcccgta
      661 ggtaatttcc gtactccgca gccatcccag aacaggcgca aacgctttgc gcttgattaa
      721 acgctcccaa gccgcattca tctgccggag tgtctcgcgc aactcaccaa cgggaggatt
      781 ttttacagtc agggtcagga ataaccaact cgcagtaggg tgctcagcca gtagtttcgg
      841 tagcgcctcc cacagacgac gcatccagcg taacgagcgg cgacggctac acaccggaca
      901 aaacgggaca tgacagaatt caccatgacg cagtgcaagt cgcatctcgc ctgtaagacg
      961 ggaactctgt ggagcgaact gtaaacgctg actgcaatca aacatcctct cgccgcgacg
     1021 gaaaaatgcc tggtctgacg cgtaaatctt tgccacctgg tcagccctcg cgcgatcgct
     1081 gtcccatttt gaatcctgcg tgctgtagtc acgcagtgag aggatcggac tatctggatt
     1141 tttcatgacc tgccctcagt aggcaggggc agaaatcttg tgttagcatg ttcttaccac
     1201 ctgtgcttgg cttaccgaat tttacctttc aggtggtcgc ccccggagtg gtagcacact
     1261 gccggggaac ctcaccaacc tgttgctcac actgtagcca aaaaaagttg taaaaacaat
     1321 ttaacaacca tctacaacta aaaaacccct caaacccgcg ccattactgg gctgaaaggc
     1381 aaaaatgcat ccgcgagttg ttgcctatag tataaataca aggcgcaccc caaaatcgct
     1441 caatatttgc gcagattttc agcaccgcaa aaccccgttt ttcgcgcctg ttacacccac
     1501 cccatccaca accttttcac ccgaatctgt gaacaacaaa aaaccgtccg gcaggtggtt
     1561 ggcctcactg cgtgacacca accaccactc ccagtgtttt tatccgccat gtcgtcaatc
     1621 acaaaatcgg aacccacgcc ggaggcgcgg gaccccgcga ttttctgttt gacgagtcag
     1681 ggaacggcga acgctgacag atttcaatct ggctgtgtga gacgttggcg gggtgtcggg
     1741 gcgcagccct gaccagatgg tgtttgtttg atggcgcgtg cgcggtccga caaacacaca
     1801 tcctgtcccg attttttcgc ctgaacgctg gcagatgggc agatgaggct cggcattttc
     1861 gacgaccgga caacaaatca ttttgccgtt tctttgagcg tcgcagaccg cacacaatcg
     1921 gaacggagtg gagattgtat aagtgcgcat ttcatgattt ttttccgcgc agcaatctgg
     1981 cgctcgtgag gtccaactcg ttcgcaaaaa ccgcaaaatg atttgccgtc acatttccga
     2041 aatgttccgt taccacgccg gagaaaatcc aaaaaaccgg aagcaaaaac gggacaggat
     2101 gtgtgtttgt cggaccgcgc acgcgccatc aaacaaacac catctggtca gggctgcgcc
     2161 ccgacaccct agacaccgtg ccagcgacac gctaaaacga caaacgcgcg tttctggcac
     2221 cccaggaaac cggaagtcca gagagagtta cagtcgacaa cgccagaaac gcgct
//
