LOCUS       NZ_CP049128             4062 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli strain EC931 plasmid pEC931_8, complete sequence.
ACCESSION   NZ_CP049128
VERSION     NZ_CP049128.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14164122
            Assembly: GCF_014522225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of a tigecycline and colistin resistant
            Escherichia coli isolate EC931
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. 3.12; Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/15/2023 02:18:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,104
            CDSs (total)                      :: 4,985
            Genes (coding)                    :: 4,684
            CDSs (with protein)               :: 4,684
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 83 of 301
            Pseudo Genes (incomplete)         :: 207 of 301
            Pseudo Genes (internal stop)      :: 51 of 301
            Pseudo Genes (multiple problems)  :: 40 of 301
            Pseudo Genes (short protein)      :: 5 of 301
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049128.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC931"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC931_8"
                     /geo_loc_name="China: Yiwu"
                     /lat_lon="29.334975 N 120.121983 E"
                     /collection_date="2017-02-10"
                     /collected_by="Jianyong Wu"
     gene            368..1069
                     /locus_tag="G6G93_RS25140"
                     /old_locus_tag="G6G93_25235"
     CDS             368..1069
                     /locus_tag="G6G93_RS25140"
                     /old_locus_tag="G6G93_25235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281396.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVEITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1317..3464)
                     /gene="mobQ"
                     /locus_tag="G6G93_RS25145"
                     /old_locus_tag="G6G93_25240"
     CDS             complement(1317..3464)
                     /gene="mobQ"
                     /locus_tag="G6G93_RS25145"
                     /old_locus_tag="G6G93_25240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_070749924.1"
                     /translation="MAIYHLSMKIISRSNGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQKEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NHLINRLNLEIQISHTELRNSGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKLEKQDIPASQTAFEKQWQQWGESGLAALRSQL
                     LKREEELRRKELEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQYPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWDEW
                     RREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            3643..3897
                     /gene="mobC"
                     /locus_tag="G6G93_RS25150"
                     /old_locus_tag="G6G93_25245"
     CDS             3643..3897
                     /gene="mobC"
                     /locus_tag="G6G93_RS25150"
                     /old_locus_tag="G6G93_25245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091744.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(3107..3465)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.5"
                     /coverage="100.0"
                     /evalue="8.52e-174"
     Typing          3466..3642
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          369..1068
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1321..3551)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.059"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgtattcgga atcttcaatc ctgatttttt tcaacacatt gtttttattg gatttttatt
       61 ttcagcaccc caagttatcc acaggctcgc cgttaagttt ttaagatctt atttttagat
      121 cttttttttt aaaacctttt taggtcttgt aaccacatga ttttaaaggt gttctggcga
      181 gataggtgga gcttttgggg gtgataggtg gagcttttgg gggtgatagg tggagctttt
      241 gggggtgata ggtggagctt ttgggggtgt aatccacaga aaaaaggggt tgcaaaaaaa
      301 aaacttccac caatatgatg ggtggcgtac agtaaaaaaa cgccaccaat agcaacgagc
      361 aagcgaaatg tcagatctga tagcttacaa atcaaacgcc cttatcgagg caagctacaa
      421 gctcactttg caggaacagc gcttcctgct ggtctgtatc agccgcctga aatccggaga
      481 agattcccct tcgccggatg aacaaaaaac catgaccatc acggctgcag agtttcatga
      541 tgcctttcct gacatgggac ggcagcacgc tgaagctagg ctcaaggaag cgatagatcg
      601 cctctgggac aggtcagtaa tcatcaagaa cgaggaaaaa cgagaagagt tccgctgggt
      661 gcagtaccgg gcgcagtatt tcaaaggtga ggcgaaagta gagatcacct tctccgatgc
      721 catcatgccg tatctgacac agctcaaggg gcagtttacg cgggtagtcg tgaagaacgt
      781 ctccagcctc tccagcacct acagcatccg catttacgag ctgctccagc agttccgcag
      841 caccggtgac agaacgatag ctatcgatga tttccgaacc atgcttgccg ttgaagataa
      901 atatcaacaa ttcaaagact tgaataagat gataatcaag ccagccatcg ccgaactgaa
      961 cgaaaaaagc gatctggttg taacggttga gacgatcaaa aaggggcgca cggttgtagc
     1021 cctgcatttt cgcttcaagg aagacaaaca gatcaagatg acgatctaga atcgatttta
     1081 aggcacctaa aacactccct gttgcgtttt ttttgtgtta gtagtgcgaa ggtactattg
     1141 aagtgaaaaa ttgcgtcaaa aacgctctga tgcagcgaaa aaagaacccc caatttttgg
     1201 gggcggaaaa acaaaactat gcttgcgtta tgtgagtgtg cttcttgcat tcatccctgt
     1261 cgatgaacat cgacgaacat aacacaatgt taaacaaaac aaaatattgt tcacattcac
     1321 tgtgaccaac cgcgatcatg tctcctctcg gtggcgtgtg ttcgtgcatc ttcctcgcgc
     1381 cgccattcgt cccaggtacg attgaaaccc agcgagcgga acagatgatc cggctgtttt
     1441 tctggtgtgt tgacggcagc aaaaagcggt gcctcacggg ttttcagatg aaactccata
     1501 agttccggcg caggtgatat gtggctgtct gcatcatgat ttacggcaac accataccct
     1561 gcataccgta accccttatc ggtcagtgca ctgatgacca gctggctgtt atcggctgag
     1621 ggaacggcaa acgcgggata cagcgtacca ttaagccgga caacaccgcc cagagagtca
     1681 tccacgccca tgaccagcaa aagctgaagc tggcgggttt cccggtcgac agaactcatg
     1741 accggcatca gcgtcggtgg cagacgtccc tgcgtaaaat ccaccttcct tgctgccagt
     1801 gatggcatgg ctggctggcg gtcttctcca agacagaaag actccggcca tgtcttcggt
     1861 tggcggagat tgcgggtatg ttgcggccat gtctggcaaa cctgcgtcag aagttgctca
     1921 aaggtcgcag aggaataaac acgttgcagt ggtggatatt gaccgatttc gcttcggtca
     1981 cagatataac cactcctgtc acgcattaaa tcaacactct caaagatcct ggtgcggtat
     2041 ttctccagct cgttccccca gagcgtatat tctccgttcc ggttgtgaag cagaaggtaa
     2101 caggtcattt ccccgttacc aggcatggga gcctcgccat accccgtaac gactgctctc
     2161 agcacccctg aatccataac ggcattgtca tttttcctga gctgctccgc gcgactacgt
     2221 tctgtatcct ccagctcctt ccggcgcaat tcctcttcac gtttaagcaa ctgggatctg
     2281 agagctgcaa ggccggattc cccccactgc tgccactgtt tttcaaatgc cgtctgactc
     2341 gcgggaatgt cctgtttctc cagcttccgg taatgctgtc gcaatttttc catttgctgg
     2401 ctcagagact ggatttttcc ctgctcacta cggtattccg gtgcagccca ttgcatcagg
     2461 ggaatacggg aaaaaccgga cgaaaacggc gatgattttt tcaggctggc agcatggctc
     2521 atcgcttcct ttcgttcggt gttcagctct tcccagcgtc tgcgcgtcgc ctgcatatct
     2581 gccatcatcg acatggcgtt actggtgagt ttccatgccg cgttactgtc cttcggcaac
     2641 gcttcgatga tgtcacgtag tgtgtacgaa gttgcgttgt caggaagccc gatattcaga
     2701 aggtcagcca cacggcgggc aggctctgtc agcccgctgt tcctgagttc ggtgtgggaa
     2761 atctgtattt ccaggttaag cctgttaatc agatgattct gttcgccgcg ttctgtttcg
     2821 ataccgcgtg tttccatcgc acaggcagac tttccgaggt gaatacccgg cgttttctca
     2881 agcccctgtc gctcgtatga acgatggtct atctctttct gataaccggc gttcgccagt
     2941 gccctgtttg catggtcagc ccatgatgca cgccacgtct cggcatgtgt ccggtcgttc
     3001 cagtccctga cctttccgcc gaacccagca ggcccgacag ccctcgtggt cagcatgatg
     3061 tgcgcatggg gatttgtttt atccatgtga tggaacgcca catcagcaat catgccctga
     3121 ctgacaaagt tttctctgac aaaggcgaga acggtttccc gtgcggcatc ctgcggcagt
     3181 tcgtggggga tggcgagttc aatttcccgc gcaagttgtg agttcttccg gcgttcggcc
     3241 ttctcaacgg cattccagag ttcggcacgg ttcgtacacc aggcaggggc tgtcgcgggg
     3301 gtcagaatca cagcctcagc cacaccgctt ttgcgggtgt agtcgtgagt cagtccggta
     3361 cgctcatcaa gcattagtga accagaacga taggcagcag aagcaacggc gctgtagccg
     3421 ttgcttctgg agatgatttt catactgaga tgataaatcg ccatttctgc cgtaatgcct
     3481 ttttgcagtt ttctttcagc cccgcagggc gcacacaatc gaaacggagt ggagattgta
     3541 taagtgcgca cttcgtgatt tgccgtgcat tatgccagct cagtttttaa actaccagtg
     3601 acgcgctaaa atgcgatttt tcatcgcgca tggagttaca tcatgagcag aacgctcgaa
     3661 cagaagattg ctgatgctga ggcgagatta cagcgcctga aggcgaagag ccggagcctg
     3721 gacacagcgc agaaggtcat tgtgggtgca gcattgctgg caaaagtcag aaagccggag
     3781 gaagtgcagt tgcgagcttg gttactccag ttcctgaagg cagaggtgac acggcaggct
     3841 gatgtgacgc gaatactgcc gctgattaac gagctggagg cgctgccaga gcagtgattg
     3901 tatcgtccgc tcgccgcagc ccagcgaccg agcgttagcg agcgagtggg cgaggaagcg
     3961 gaagtgaatc agtcgccatg ttgagcacat acgcacggtt tttcgacagg tgatatgcgc
     4021 tgaacccgca tgaatactgg atttccacag ggttggaggg gg
//
