LOCUS       NZ_CP049127             4069 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli strain EC931 plasmid pEC931_7, complete sequence.
ACCESSION   NZ_CP049127
VERSION     NZ_CP049127.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14164122
            Assembly: GCF_014522225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of a tigecycline and colistin resistant
            Escherichia coli isolate EC931
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. 3.12; Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/15/2023 02:18:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,104
            CDSs (total)                      :: 4,985
            Genes (coding)                    :: 4,684
            CDSs (with protein)               :: 4,684
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 83 of 301
            Pseudo Genes (incomplete)         :: 207 of 301
            Pseudo Genes (internal stop)      :: 51 of 301
            Pseudo Genes (multiple problems)  :: 40 of 301
            Pseudo Genes (short protein)      :: 5 of 301
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049127.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC931"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC931_7"
                     /geo_loc_name="China: Yiwu"
                     /lat_lon="29.334975 N 120.121983 E"
                     /collection_date="2017-02-10"
                     /collected_by="Jianyong Wu"
     gene            join(2564..4069,1..639)
                     /gene="mobQ"
                     /locus_tag="G6G93_RS25125"
                     /old_locus_tag="G6G93_25220"
     CDS             join(2564..4069,1..639)
                     /gene="mobQ"
                     /locus_tag="G6G93_RS25125"
                     /old_locus_tag="G6G93_25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_187976031.1"
                     /translation="MAIYHLSMKIISRSNGYSAVASAAYRSSSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQKEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NHLINRLNLEIQISHTELRNSGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKLEKQDIPASQTAFEKQWQQWGESGLAALRSQL
                     LKREEELRRKELEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQHPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGENRQPAMPSPVARRVDFTQGRLPPTLMPVM
                     SSVDRERQLQLLLVMGVDDSLGGVVRLNGSLYPAFAVPSADNRHLVISALTDKGLRYAG
                     YGVAVNHDADNHISPAPELMAFHLKTRETPLFAAVNTPEKQPDHLFRSLGFNHTWDEWR
                     REEHARTRVSGKKNDRDCSR"
                     /name="mobQ"
     gene            complement(894..1601)
                     /locus_tag="G6G93_RS25130"
                     /old_locus_tag="G6G93_25225"
     CDS             complement(894..1601)
                     /locus_tag="G6G93_RS25130"
                     /old_locus_tag="G6G93_25225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001296720.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVASP
                     ELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRSTGE
                     RIIALDDFKSSLMLDGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTVVALH
                     FRFKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(2131..2385)
                     /gene="mobC"
                     /locus_tag="G6G93_RS25135"
                     /old_locus_tag="G6G93_25230"
     CDS             complement(2131..2385)
                     /gene="mobC"
                     /locus_tag="G6G93_RS25135"
                     /old_locus_tag="G6G93_25230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091744.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(2387..2563)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.315"
                     /coverage="100.0"
                     /evalue="5.65e-83"
     Typing          2564..2922
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.667"
                     /coverage="100.0"
                     /evalue="8.58e-169"
     Typing          complement(895..1601)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.294"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..636
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.263"
                     /coverage="100.0"
                     /evalue="1.2e-118"
     Typing          2565..4069
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.245"
                     /coverage="83.0"
                     /evalue="0.0"
ORIGIN
        1 catccaccac tgcaacgtgt ttattcctct gcgacctttg agcaacttct gacgcaggtt
       61 tgccagacat ggccgcaaca tacccgcaat ctccgccagc cgaagacatg gccggagtct
      121 ttctgtcttg gagaaaaccg ccagccagcc atgccatcac cggtggcaag gagggtggat
      181 tttacgcagg gacgtctgcc accgacgctg atgccggtaa tgagttctgt cgaccgggaa
      241 cgacaacttc agcttttgct ggtcatgggc gtggatgact ctctgggcgg tgttgtccgg
      301 cttaatggtt cgctgtatcc cgcgtttgct gtcccctccg ccgataacag gcatctggtc
      361 atcagtgcgc tgaccgataa ggggttacga tatgcaggat atggtgttgc cgtaaatcac
      421 gatgcagaca accacatttc acctgcaccg gaacttatgg cgtttcatct taaaacccgc
      481 gagacaccgc tttttgctgc cgtcaacaca ccagaaaaac agccggatca tctgttccgt
      541 tcactgggct ttaatcacac ctgggatgaa tggcggcgcg aggagcatgc acgaacacgt
      601 gtctccggga agaagaatga tcgcgactgt tcacggtaac tgctgatagt attttgtttt
      661 gttttacatc gtgttatgtt ctctgacatt catcgtaact ggatgaatgt cagatgcaca
      721 cagattgcac aaatccaact ttatcgtttt cccgccccca ctaagttggg ggttcttttt
      781 tttgaacgcc atagaatcga ttatgaagca attttatctc accagtagta ccatcgcata
      841 cctctcatgg aaaaaacgca acagagagcg ttttaggtgc cttaaaatcg attctagatc
      901 gtcatcttga tctgtttgtc ttccttgaag cgaaaatgca gggctacaac cgtgcgcccc
      961 tttttgatcg tctcaaccgt tacagccagg tcgctttttt tgttcagttc gtcaacacag
     1021 ggttttatta gatccctgtt taacgttttg aaatctttat atttaccatc aagcatcagt
     1081 gatgatttaa aatcatcgag ggcgataata cgttccccag tactgcggaa ttgctggaga
     1141 atctcgtata ttcttatgct gtaacttctt gataaattgg agatattttt aataacaact
     1201 ctggtgaatt gccccttcaa ctgtgtcaga taaggcatta ccgcgtcaga aaaagtgatt
     1261 tgtgctttcc cctctcccct cgcgtactgt gcccgatact gaatccagcg gaactcctcg
     1321 cgcttctcgt cgtccttgag aatgatcgac ctgtcccaca aacgatcgat cgcttcctgt
     1381 agctgaacct cggcattttt ccgccccata tcagggaatg aatcaaaata ttccgcagct
     1441 gtaatggtca ttgtcttttg caattcaggt gaagctacgt cagtaccgga gttaagtcgg
     1501 cttatgcaga gcaataagaa acgctgttct tgtaaagtta gcttgtaact ggcctcaacc
     1561 agcgcattgg acttataggc tatcagaggg tttgatgtca ttttaaccac caggccaaag
     1621 atgaggtgag ataaaaacct atcaccttta gcattggtga gcaattatat ttccaccccc
     1681 catttttctg tgaatattac cttaaatctc ctcaccttta ccttaaatta cctcacccaa
     1741 gaaccttaaa ttatatcacc tttaccttaa atttccccac ctatacacgt gtaacgcatt
     1801 gtttttaatc aggaatttcg accctaaaac aagtattaaa aacaagtatt aaaaacaaga
     1861 tctaaaaact taacggcgag cctgtggata acttggagtg ctgaaaataa aaacccaata
     1921 aaaacaatgt gttgcaaaaa atcaggattg gagattccga ataccccccc tccaaccctg
     1981 tggaaatcca gtattcatgc gggttcagcg catatcacct gtcgaaaaac cgtgcgtatg
     2041 tgctcaacat ggcgactgat tcacttccgc ttcctcgccc actcgctcgc taacgctcgg
     2101 tcgctgggct gcggcgagcg gacgatacaa tcactgctct ggcagcgcct ccagctcgtt
     2161 aatcagcggc agtattcgcg tcacatcagc ctgccgtgtc acctctgcct tcaggaactg
     2221 gagtaaccaa gctcgcaact gcacttcctc cggctttctg acttttgcca gcaatgctgc
     2281 acccacaatg accttctgcg ctgtgtccag gctccggctc ttcgccttca ggcgctgtaa
     2341 tctcgcctca gcatcagcaa tcttctgttc gagcgttctg ctcatgatgt aactccatgc
     2401 gcaatgaaaa atcgcatttt agcgcgtcac tggtagttta aaaactgagc tggcataatg
     2461 cacggcaaat cacgaagtgc gcacttatac aatttccact ccgtttcaat tgtgtgcgcc
     2521 ctgcggggct gaaagaaaac tgcaaaaagg cattacggca gaaatggcaa tttatcatct
     2581 cagtatgaaa atcatctcca gaagcaacgg ctacagcgcc gttgcttctg ctgcctatcg
     2641 ttctagttca ctaatgcttg atgagcgtac cggactgact cacgactaca cccgcaaaag
     2701 cggtgtggct gaggctgtga ttctgacccc cgcgacagcc cctgcctggt gtacgaatcg
     2761 tgccgaactc tggaatgccg ttgagaaggc cgaacgccgg aagaactcac aacttgcgcg
     2821 ggaaattgaa ctcgccatcc cccacgaact gccgcaggat gccgcacggg aaaccgttct
     2881 cgcctttgtc agagaaaact ttgtcagtca gggcatgatt gctgatgtgg cgttccatca
     2941 catggataaa acaaatcccc atgcgcacat catgctgacc acgagggctg tcgggcctgc
     3001 tgggttcggc ggaaaggtca gggactggaa cgaccggaca catgccgaga cgtggcgtgc
     3061 atcatgggct gaccatgcaa acagggcact ggcgaacgcc ggttatcaga aagagataga
     3121 ccatcgttca tacgagcgac aggggcttga gaaaacgccg ggtattcacc tcggaaagtc
     3181 tgcctgtgcg atggaaacac gcggtatcga aacagaacgc ggcgaacaga atcatctgat
     3241 taacaggctt aacctggaaa tacagatttc ccacaccgaa ctcaggaaca gcgggctgac
     3301 agagcctgcc cgccgtgtgg ctgaccttct gaatatcggg cttcctgaca acgcaacttc
     3361 gtacacacta cgtgacatca tcgaagcgtt gccgaaggac agtaacgcgg catggaaact
     3421 caccagtaac gccatgtcga tgatggcaga tatgcaggcg acgcgcagac gctgggaaga
     3481 gctgaacacc gaacgaaagg aagcgatgag ccatgctgcc agcctgaaaa aatcatcgcc
     3541 gttttcgtcc ggtttttccc gtattcccct gatgcaatgg gctgcaccgg aataccgtag
     3601 tgagcaggga aaaatccagt ctctgagcca gcaaatggaa aaattgcgac agcattaccg
     3661 gaagctggag aaacaggaca ttcccgcgag tcagacggca tttgaaaaac agtggcagca
     3721 gtggggggaa tccggccttg cagctctcag atcccagttg cttaaacgtg aagaggaatt
     3781 gcgccggaag gagctggagg atacagaacg tagtcgcgcg gagcagctca ggaaaaatga
     3841 caatgccgtt atggattcag gggtgctgag agcagtcgtt acggggtatg gcgaggctcc
     3901 catgcctggt aacggggaaa tgacctgtta ccttctgctt cacaaccgga acggagaata
     3961 tacgctctgg gggaacgagc tggagaaata ccgcaccagg atctttgaga gtgttgattt
     4021 aatgcgtgac aggagtggtt atatctgtga ccgaagcgaa atcggtcaa
//
