LOCUS       NZ_CP049126             4548 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli strain EC931 plasmid pEC931_6, complete sequence.
ACCESSION   NZ_CP049126
VERSION     NZ_CP049126.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14164122
            Assembly: GCF_014522225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of a tigecycline and colistin resistant
            Escherichia coli isolate EC931
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. 3.12; Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/15/2023 02:18:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,104
            CDSs (total)                      :: 4,985
            Genes (coding)                    :: 4,684
            CDSs (with protein)               :: 4,684
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 83 of 301
            Pseudo Genes (incomplete)         :: 207 of 301
            Pseudo Genes (internal stop)      :: 51 of 301
            Pseudo Genes (multiple problems)  :: 40 of 301
            Pseudo Genes (short protein)      :: 5 of 301
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049126.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC931"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC931_6"
                     /geo_loc_name="China: Yiwu"
                     /lat_lon="29.334975 N 120.121983 E"
                     /collection_date="2017-02-10"
                     /collected_by="Jianyong Wu"
     gene            complement(142..852)
                     /locus_tag="G6G93_RS25105"
                     /old_locus_tag="G6G93_25200"
     CDS             complement(142..852)
                     /locus_tag="G6G93_RS25105"
                     /old_locus_tag="G6G93_25200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_053272217.1"
                     /translation="MLYWKKMPSLWIGNRLTEFNSLETSHAIAAIKIYITFCLFGKEED
                     DGVRTVKLTFTEISEIASISRSLVNEGLKLLDNKKLIKYLSPASKRKKIYTVDVDGCIH
                     DGWCKLPMAGIVSEDRTISAFQSMHNRYHFELVALQLYLYLLYARDNFQEHVLARKQTI
                     CEKLACRNVDLNKAITYLIHIGLLKGIKQKAVESYPIELFHDSFYFYVKTGASNALTFK
                     KQPLVNPPEKEIPY"
                     /name="hypothetical protein"
     gene            complement(1198..1797)
                     /locus_tag="G6G93_RS25110"
                     /old_locus_tag="G6G93_25205"
     CDS             complement(1198..1797)
                     /locus_tag="G6G93_RS25110"
                     /old_locus_tag="G6G93_25205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657475.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_001556731.1"
                     /translation="MRLKLTCCISNDTTGIKWDIEMVYRAMDNSQIGTPESHEQDNYNF
                     VQVSRAYLKEWRALTRKSPIASEILMYLIENMGRTTNAVVCSYTTLMEITGVSRASVAR
                     AVKILKEDRWMQAVKIGNATAYCVNAKAFWQAGRNQKKYAIFQATVIASSSEQESDFLE
                     KSQQDLRYIPFVDLKDRPIVGTDELPPPDQTDLDLD"
                     /name="replication/maintenance protein RepL"
     gene            2315..3283
                     /gene="mobV"
                     /locus_tag="G6G93_RS25115"
                     /old_locus_tag="G6G93_25210"
     CDS             2315..3283
                     /gene="mobV"
                     /locus_tag="G6G93_RS25115"
                     /old_locus_tag="G6G93_25210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852935.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_053272216.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNADADRTPENHCS
                     VSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWNEATPRQQAEFFARSEQWLE
                     NKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMRDDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSQAVNDGFAGTIAMASQSAQNAKRARDLQKTMDSQQKRLQSVTEPFKGLSREQM
                     THILTMAQTFQQQNHDREKQRQLEREQERQQRQKTDRGISR"
                     /name="mobV"
     gene            3422..4402
                     /locus_tag="G6G93_RS25120"
                     /old_locus_tag="G6G93_25215"
     CDS             3422..4402
                     /locus_tag="G6G93_RS25120"
                     /old_locus_tag="G6G93_25215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.2"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="WP_213091166.1"
                     /translation="MSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELTEVDVDWLIAERPGKVRTLKQHPRKNKTAINIEYMKASIRAKVEHPFRI
                     IKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSH"
                     /name="IS5-like element IS5 family transposase"
     Typing          2286..3178
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.289"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 ctagtggagt acctcgttat gaatgtttga aaacgaggat tatctctggc gaaacaagtt
       61 atgaacaagg gcgtaaagaa atattggatt actaccttag aataaatcat aagggcgagg
      121 agtaattcgc cctcaggtat gtcagtacgg aatttctttt tctggagggt taacaagagg
      181 ttgctttttg aatgtaagcg cattgcttgc tcctgttttt acataaaaat aaaatgaatc
      241 atgaaaaagt tctatgggat aagactccac tgccttttgt ttaattcctt ttaaaagacc
      301 tatgtgtatt aaatatgtta ttgctttatt taaatctaca ttgcggcaag caagcttttc
      361 gcaaatagtt tgttttctag ctaacacatg ctcctggaag ttatctctgg catatagaag
      421 gtataaatat agctgaagcg ctacaagctc aaaatggtat ctattatgca ttgactggaa
      481 ggcgctgatt gttctgtctt cgctaacgat gcctgccata ggcagcttac accatccgtc
      541 atgaatgcac ccatcaacat ccactgtata gattttcttt ctttttgacg cgggcgatag
      601 gtatttaatt agttttttat tatcgagcaa tttgagacct tcatttacca aagatcggct
      661 gatagaagct atttcgctta tttctgtaaa cgtaagtttt acggttcgca ctccatcatc
      721 ctcttctttt ccaaagagac aaaaagttat atatatcttt atggcggcaa tggcatgtga
      781 agtctctaaa ctattaaact ctgtcagtct attaccgatc cagagtgatg gcattttttt
      841 ccaatataac attagtgatt cctttatgcg gaaatgaaag tgaaataaat cataattaac
      901 acacaaatgg ccaatgcaag actagtaagt gaccctgcca cttttcccag cctttcagca
      961 agctctacaa atcgctctaa gtcacccatt ttgacctctg ctcctgttta ccgatatacc
     1021 gattatccca gtaatccgcg tatctgtatc tataggagca aaaatctatc tagaaaggta
     1081 tgggaaaaaa ttaatatgaa tttttataat taaatcaaaa agttaactta aaaaaatggt
     1141 tatcaaatga acttcaatat ggttcacggt gaaccatatt tgaaggtttt cagaggctca
     1201 gtcgagatct agatctgttt gatccggagg cgggagttca tcggtgccaa caatagggcg
     1261 gtctttgaga tcgacaaaag gaatatatcg aagatcttgc tgtgattttt ctaagaaatc
     1321 agactcttgc tctgaggagg aggcaatcac ggtagcttgg aagattgcgt attttttttg
     1381 atttctacct gcctgccaaa atgctttggc attaacacaa tatgctgtgg cattacctat
     1441 ttttactgcc tgcatccatc gatcttcctt caggatttta accgcccttg ctactgatgc
     1501 tctagagacc ccggttatct ccatcaaagt cgtatagctg caaacaacag cattagttgt
     1561 cctgcccata ttctcaatca agtacataag gatttcagag gcaatagggc ttttccttgt
     1621 cagcgcacgc cattccttga gatacgcgcg gctaacctgg acgaaattat agttatcctg
     1681 ctcatgagac tctggagtgc caatctgtga attatccatt gctctataga ccatttcaat
     1741 atcccatttg ataccggttg tatcattaga gatgcaacag gtcaacttga gtctcatagc
     1801 tagtgagact cataagtctc atggctggtg agactccccc tttttttaac ccaatataat
     1861 cagcgattta ctgaaagctc cttttctata tctattaagt ccgtgcatac gtagcccaat
     1921 gtacgcctag tggacattcc gcttcctcgt tcaggctgtt cgttcctcgc tgcgctgcgg
     1981 tacgcctcag cctgacctgc ggcgagcggc acgtgctctt tcaaagacga ctttgttgcc
     2041 tgacattcgt caagggcgag cgtagcgagt gccatttacc cttgacgact caccggataa
     2101 aacaggcttg gcagtggtgg ggggtggcgc ggagcgacgc cttccacctg ccagactgcc
     2161 gttgattttg accttctggc agcttgctgc ccgaacgatt ttgcgtagca aagtctagtg
     2221 agtacactat catcctgata gtggcccgcc ggaggcatcg caatgcgatc ccccgtctgg
     2281 ccggatggac gccggaacga gggggggggg aagcatggcg aactacgcga taatgcgctg
     2341 taaaaagctg acgggaatgg gcagtgtggc cagcgcgtta cagcactgct acagggagag
     2401 agaaacgccg aatgctgatg ctgaccggac accagaaaac cactgctctg tgtctcagtc
     2461 ggcagatgag gcgatgggaa agctgcggga gctgttgccg gaaaaacgcc gtaaagatgc
     2521 ggtactggct gttgaatacg ttatgacagc aagccctgag tggtggaacg aggccacacc
     2581 gcggcagcag gcggagtttt tcgcccgttc tgagcaatgg ctggagaaca aatacgggaa
     2641 agatcgcgtt gtggcggcgg tggttcacag ggacgaggcc accccgcacc tgtctgcgtt
     2701 cgtggtgcca ttgacgcagg atgggcggct gtccgcgaaa gagtttattg gcgggcggtc
     2761 aaagatgcgt gacgatcaga gcacctacgc tgaatcggta aaaaaactgg gtcttgagcg
     2821 cggcatcgag ggaagccgtg ccacgcatca gacagttcag cactactacg aatcgataaa
     2881 ccgtggcacc cgtagccagg tatcgatctc accggaggcg ctggagccgc gtgtactgcg
     2941 gaagggtatt ttcaccaaag acgtggaaga tcaggcagcc atagccaaac gtttaagcca
     3001 ggcagtgaac gatggctttg caggaaccat cgctatggcc tctcagagcg cccagaatgc
     3061 caaaagagcg cgcgatctac agaagactat ggattcgcag caaaaacgcc tacagagcgt
     3121 tacagagcct tttaaaggac tttcccggga gcagatgacc catattctga cgatggcgca
     3181 gacgttccag caacagaatc acgatcgtga aaaacagcgc cagcttgaac gggagcagga
     3241 acgccagcaa cgtcagaaaa ctgacagagg tattagccga tgagtgaaaa gcagcgccgt
     3301 gaagggtttc gaaaagcgga agcatcacta agacttgagg gaatggatcc tagggaaggt
     3361 gcgaacaagt ccctgatatg agatcatgtt tgtcatctgg agccatggaa cagggttcat
     3421 catgagtcat caacttacct tcgccgacag tgaattcagc agtaagcgcc gtcagaccag
     3481 aaaagagatt ttcttgtccc gcatggagca gattctgcca tggcaaaaca tggtggaagt
     3541 catcgagccg ttttacccca aggctggtaa tggccggcga ccttatccgc tggaaaccat
     3601 gctacgcatt cactgcatgc agcattggta caacctgagc gatggcgcga tggaagatgc
     3661 tctgtacgaa atcgcctcca tgcgtctgtt tgcccggtta tccctggata gcgccttgcc
     3721 ggaccgcacc accatcatga atttccgcca cctgctggag cagcatcaac tggcccgcca
     3781 attgttcaag accatcaatc gctggctggc cgaagcaggc gtcatgatga ctcaaggcac
     3841 cttggtcgat gccaccatca ttgaggcacc cagctcgacc aagaacaaag agcagcaacg
     3901 cgatccggag atgcatcaga ccaagaaagg caatcagtgg cactttggca tgaaggccca
     3961 cattggtgtc gatgccaaga gtggcctgac ccacagccta gtcaccaccg cggccaacga
     4021 gcatgacctc aatcagctgg gtaatctgct gcatggagag gagcaatttg tctcagccga
     4081 tgccggctac caaggggcgc cacagcgcga ggagctgacc gaggtggatg tggactggct
     4141 gatcgccgag cgccccggca aggtaagaac cttgaaacag catccacgca agaacaaaac
     4201 ggccatcaac atcgaataca tgaaagccag catccgggcc aaggtggagc acccatttcg
     4261 catcatcaag cgacagttcg gcttcgtgaa agccagatac aaggggttgc tgaaaaacga
     4321 taaccaactg gcgatgttat tcacgctggc caacctgttt cgggcggacc aaatgatacg
     4381 tcagtgggag agatctcact aaaaactggg gataacgcct taaatggcga agaaacggtc
     4441 taaataggct gattcaaggc atttacggga gaaaaaatcg gctcaaacat gaagaaatga
     4501 aatgactgag tcagccgaga agtatttccc cgcttattcg caccttcc
//
