LOCUS       NZ_CP049124             7462 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli strain EC931 plasmid pEC931_4, complete sequence.
ACCESSION   NZ_CP049124
VERSION     NZ_CP049124.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14164122
            Assembly: GCF_014522225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of a tigecycline and colistin resistant
            Escherichia coli isolate EC931
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7462)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. 3.12; Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/15/2023 02:18:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,104
            CDSs (total)                      :: 4,985
            Genes (coding)                    :: 4,684
            CDSs (with protein)               :: 4,684
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 83 of 301
            Pseudo Genes (incomplete)         :: 207 of 301
            Pseudo Genes (internal stop)      :: 51 of 301
            Pseudo Genes (multiple problems)  :: 40 of 301
            Pseudo Genes (short protein)      :: 5 of 301
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049124.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC931"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC931_4"
                     /geo_loc_name="China: Yiwu"
                     /lat_lon="29.334975 N 120.121983 E"
                     /collection_date="2017-02-10"
                     /collected_by="Jianyong Wu"
     gene            complement(join(6668..7462,1..141))
                     /locus_tag="G6G93_RS25045"
                     /old_locus_tag="G6G93_25130"
     CDS             complement(join(6668..7462,1..141))
                     /locus_tag="G6G93_RS25045"
                     /old_locus_tag="G6G93_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_011666805.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDKRFMAL
                     CMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
                     /name="replication initiation protein"
     gene            514..960
                     /locus_tag="G6G93_RS25370"
                     /old_locus_tag="G6G93_25135"
     CDS             514..960
                     /locus_tag="G6G93_RS25370"
                     /old_locus_tag="G6G93_25135"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_137508464.1"
                     /translation="MEGSARRFSVSELHVSPGQKKTDQGSGQESESVRNFFSCGMRQCS
                     QPPQNTLRLIKNNWHYPVPDKKTTKVGNPRETIGVRLSQNIDNEIVTQINLAASLILIQ
                     YSIKNARIINHIYKDIYQTKQPSSQLRIRIFLYVIDKTQFFYKL"
                     /name="hypothetical protein"
     gene            complement(1452..3110)
                     /locus_tag="G6G93_RS25050"
                     /old_locus_tag="G6G93_25140"
     CDS             complement(1452..3110)
                     /locus_tag="G6G93_RS25050"
                     /old_locus_tag="G6G93_25140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007372656.1"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_001529697.1"
                     /translation="MKIVIVGGVAGGASAAARARRLSEDVSIVVFERGSDVSFANCGLP
                     YHIGGKIPLRQSLILKTPEDFKSRFNIDVRIRHEVMSVDPVNKMVTVKNLTSGEVYTEE
                     WDRLLLSTGAAPVVPPLPGLQEAGVFTLRNLTDMDAIQAWIEQHNIAHTTLVGGGFIGL
                     EVMEALSERGISVTLLEMGEQVMAPVDPEMASALHQEIRSHGVDLRLRTALTEVLRTET
                     GFRVALSDGGFLQTDMVILAIGVKPESSLATGAGLAVGKRGGISVNACMQTSIPDIYAV
                     GDAVETPDLVFQEPANVPLAGPANRQGRIAADNMLDRHSLYHGSQGTSICKVFSLSIGS
                     VGANEKQLKAHGTRYEKVYVHAADHASYYPGATMISLKLLFSPDTGKILGAQASGKKGV
                     DKRIDVLAVVQRAGLTVNDLEHLELTYAPPFNSARDVVNQAGMVAANVIKGDTVICHVR
                     DVLQREPGSYCLLDIRSPAELKQFGEYPDALSIPLDSLRENLDKLPEDKEIIIGCQSGL
                     RGHVAYRMLQSHGFRTYNLSGGFITWQAVTESMSQ"
                     /name="FAD-dependent oxidoreductase"
     gene            3271..3618
                     /locus_tag="G6G93_RS25055"
                     /old_locus_tag="G6G93_25145"
     CDS             3271..3618
                     /locus_tag="G6G93_RS25055"
                     /old_locus_tag="G6G93_25145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329508.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_023144239.1"
                     /translation="MPMNEDTKRQTDMKNAALGAADVLRGLANSDRLLLMCQLSQGEAN
                     VTELEAAVGITQPTLSQQLGVMRRLKLVETRRAGKMVYYRIGDNRIMTLLNTLYDLYCP
                     QADPDKVKKQP"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            4162..4383
                     /locus_tag="G6G93_RS25060"
                     /old_locus_tag="G6G93_25150"
     CDS             4162..4383
                     /locus_tag="G6G93_RS25060"
                     /old_locus_tag="G6G93_25150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023144240.1"
                     /translation="MTKLDENIPHIIDAAVDFMASSQAFREYLKKLPPDNTIPPDLTDE
                     HVPLYIRRLEYYRLLYRPKQTESYWKKR"
                     /name="hypothetical protein"
     gene            complement(4437..4970)
                     /locus_tag="G6G93_RS25065"
                     /old_locus_tag="G6G93_25155"
     CDS             complement(4437..4970)
                     /locus_tag="G6G93_RS25065"
                     /old_locus_tag="G6G93_25155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_011666803.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAIEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(4973..5899)
                     /locus_tag="G6G93_RS25070"
                     /old_locus_tag="G6G93_25160"
     CDS             complement(4973..5899)
                     /locus_tag="G6G93_RS25070"
                     /old_locus_tag="G6G93_25160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_011666804.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIDRTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARMVGHDIREWLDTPVKQHELVRRLQHDVAREHCFD
                     MRDGVSRENRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            6076..6474
                     /locus_tag="G6G93_RS25075"
                     /old_locus_tag="G6G93_25165"
     CDS             6076..6474
                     /locus_tag="G6G93_RS25075"
                     /old_locus_tag="G6G93_25165"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016249654.1"
                     /translation="MRISNVEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQENERKQTIQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQELKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     Typing          complement(6669..7462)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.484"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(4977..5899)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.078"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgcatgaggc tggttctgct ggatataccg tgcaagaagc gcgcgccctt tgccggagat
       61 acgaaggccg aaagcgagat catccgtgtg atagggacgg tggggtaaat tttcttcgaa
      121 gtattgaagc gcggcgctca tgtgttacga ttccttccag gttatcgacc gacatcgccc
      181 ctgtattttc gtgaaagtct tggcggatag acacgaattt caggggcgtt tgtttttcag
      241 tcgcttacac tacacccaac ggcaaatcct gtcgatcctt cccgaaaaac cgatccattt
      301 accataaatc ccgttgtacg ccccccttaa aacgccctca gtcgctttct ggcgcaaaaa
      361 acgcaaaaag acaagaaaac accaggcaaa accagaaaaa cgcgtcagaa tcgttttcag
      421 ggcgttgcat aactatgcac gaaagcgatg tgcgatcggt ttcgtgcgtt ctgaacaccg
      481 aacggctcaa aacgaaaagt tttctccccc cggatggaag gttctgcccg gagattttct
      541 gtttcagagc ttcacgtttc gccaggacaa aaaaaaaccg accaagggtc ggggcaagaa
      601 tcagagtctg tgagaaattt ttttagttgt ggaatgcgtc aatgttcgca accgccccaa
      661 aataccctga ggctgataaa aaacaactgg cactatcccg ttccggataa aaaaaccaca
      721 aaagtgggga atccaagaga aacgataggt gtcagattga gtcagaatat tgacaatgaa
      781 atagttacgc aaataaattt agctgcatca cttattctga ttcaatactc aataaaaaat
      841 gcccgcataa taaaccacat ttacaaagat atatatcaaa cgaaacaacc aagttcacag
      901 ctcaggataa gaatttttct ctatgtaata gataaaacac agttttttta taaactgtaa
      961 tatatgagct gtaatattca tgtattaatt gtttttttga aaagcatcac aaatttattt
     1021 tttacgcata ctgccctatt gcaaccagca atggttttca cttattttca acatctcatt
     1081 taatacgagt tatacttcaa cccgtttaaa gtgcaacact aactgagtat catttgtttt
     1141 ccttacctac ccctggattc ccaggggttt tttataacta caaaccagca caacaaaaaa
     1201 acaaaacaca ataaaaacaa tgaattatat aaaactcaac gatatctttg tacggagatt
     1261 tacattcagg aacattgggg tcagtttgga tatagagaat tattgtacgg taagcctgtt
     1321 ttttgaacag tccacattat cgcaataggg taccatccag ggtgttaatt atttttgtag
     1381 atttctattc catataatac gttttcaatg aatctggaag atgaggtgcc atgtgtatgg
     1441 cacctcatct cttattgact cattgattcc gtaaccgcct gccaagttat aaagccacca
     1501 ctcagattat atgtccggaa gccgtgagac tgaagcatac ggtatgccac atgcccccgc
     1561 agaccactct gacacccgat aataatctct ttatcttcag gcaacttatc aagattctcc
     1621 cgcagggaat caagagggat ggatagcgcg tcaggatatt caccaaattg tttcagttct
     1681 gcaggagaac gaatatcaag taaacaataa ctgcccggtt cccgttgtaa cacatcgcgg
     1741 acatgacaga tgaccgtatc gcccttgata acatttgccg ccaccattcc agcctggtta
     1801 acgacgtccc ttgcactgtt gaaaggcgga gcataggtca gctccagatg ctcgagatcg
     1861 ttaacggtaa gccccgcgcg ctgtacgaca gccaggacat cgatgcgttt atcaacccct
     1921 tttttccctg acgcctgggc cccgagaatt ttaccggtat cggggctgaa cagcagtttc
     1981 agactgatca ttgttgcccc cgggtagtaa ctggcatgat cagcggcatg gacgtaaacc
     2041 ttctcgtatc gggtaccatg ggctttcagc tgcttttcat tcgccccgac actgccaata
     2101 ctcagcgaaa ataccttaca aatggatgtc ccctgactgc catggtaaag gctgtgacga
     2161 tcgagcatat tatcagcagc aatgcgtccc tggcggttgg ctggccctgc cagaggaaca
     2221 ttagcgggtt cctgaaagac aaggtcaggt gtttcaactg catcccccac ggcatagatg
     2281 tcagggatac ttgtctgcat acaggcattg acacttatgc ctccccgttt acccacggcg
     2341 agcccggctc ctgtggcaag gctgctctca ggttttaccc cgatggcgag aataaccatg
     2401 tcggtctgca gaaacccacc gtcagacagt gcaacgcgga atcccgtctc tgtccgcagt
     2461 acctcggtga gcgccgttct gagacgcaga tccacgccat gactcctgat ttcctgatgc
     2521 agggccgatg ccatttcagg atcaaccgga gccataacct gttcccccat ctccagcagg
     2581 gtcacactga tccccctttc gctcagtgcc tccatcactt cgagcccgat aaatcccccc
     2641 ccgacaagcg tggtgtgagc gatattatgt tgctcaatcc acgcctggat agcatccatg
     2701 tcagtaagat ttcgcagtgt aaagacaccc gcttcctgta gtccgggcaa ggggggaaca
     2761 accggagcgg caccggtact gaggagcaga cgatcccatt cttcggtata gacctccccg
     2821 gaggtcagat ttttaaccgt caccatctta ttaaccgggt ctactgacat cacttcatga
     2881 cggatacgga cgtctatgtt aaaacgggat ttaaaatcct ccggggtttt cagaatcagc
     2941 gactgccgta acggaatttt tccgcctata tgataaggca atccacagtt ggcgaaagag
     3001 acatcgcttc cccgctcaaa cacaacaata ctgacatcct ctgataaccg acgagccctg
     3061 gctgcagcgg atgctccccc ggcaacacca ccaacaatca ctattttcat tacatcctct
     3121 ctgttcactc ggggcaaaat atctgccata tataattagt tacccggtcc agctggacgc
     3181 tgcagacctg aagatcacgg tggcaaaatt acgccactat cattatactg aacaatatta
     3241 tataaaaata cataatataa gaaaccagca atgcccatga atgaagacac aaaacgtcag
     3301 actgatatga aaaatgcagc cctgggggca gcagatgtcc tacggggact ggccaacagc
     3361 gaccggctat tgttgatgtg tcagttaagc cagggggaag cgaatgtgac cgagctggaa
     3421 gcggcggtcg gtatcactca gccgaccctg tctcagcagt tgggtgtcat gcgtcgcctg
     3481 aagctggtcg agacccgacg ggcaggtaaa atggtatatt accgtatcgg ggataaccgc
     3541 atcatgacgt tattgaatac cttatatgat ctctactgcc cgcaggcgga ccccgacaag
     3601 gtcaaaaaac aaccttaacg tacaataatt ttcgatatcc aaactgcccc caacattcac
     3661 cagcacagtt agacattaac aatgcaaaat caatgcgatt ccggtgccgt atgtcaggga
     3721 gaattatgta aacggggggg ggccggaatc gcattccagc tcatatcatt aaatacagaa
     3781 aaaaacctct gagaacacag aggggataag gaatatgaat actccggtca ggatattatc
     3841 ctagctttta tatacaacat gctcacaagg acaacaacta gctagttaat agatattaaa
     3901 aattttagag ctgaaacctt aaagcttgct tccattccat gttttattat aaaccttatc
     3961 ttgaaaactg aaaggaagta cggcagatta attaagttgc gtgcattctt ttcacgttat
     4021 aatacaaaca agaacacctg cgaagttatt tctccttaca tatcccccta taccaacggg
     4081 ggattttttc tggttcacta acgcataaat aatagtgaca tactataatt cacgaaccgt
     4141 gataatatca gatcaatcat catgacaaaa ttagatgaaa atatcccaca tattatcgat
     4201 gcagctgtag attttatggc atcaagccag gcatttcgtg agtatctgaa aaaactcccc
     4261 cctgataaca caataccgcc agatcttact gatgagcacg taccgttata tatacgaagg
     4321 ctggaatact atcgactgct ttatcgacca aagcagacag aatcatattg gaaaaaacgg
     4381 tgaacggttc gcgaaatcct ttcgcgctcc gtgaggcgtt ggacaagcga agcgcgtcag
     4441 tttttccggc tcttccccgt gacaggtttc tccaccagtt tatcttccag tgtcacaact
     4501 ttctgataca ccgccgctgt ggcgttgtag ttcctgtcga gtattgtttt cattccccat
     4561 ccgagcacac agaacataac cagaaaagcc actgacaccc cacccagcca tttccagcta
     4621 taccctttaa gggtgttgta ttcctgcacg atattgctgt gaattgtcgc aatatccctg
     4681 ttcagggcat ccgcgtttgc cttcatgttt gcgactgact cttccagccg ctgaaaatcc
     4741 tggctgatgc gctcacctat cagtcctgcc acgtccagct tctgcacctc gtttctgact
     4801 tcatccctga cctgttttgc cacaccgtca gaaatatccc gatatccgcg aagagtttcc
     4861 cttgccacag ccagagaatc agcaacagct ttctgttgct ctattgcggt atgttgcaga
     4921 tgctccaccg ataacaggag cgtgagtaat ttttcttctg tgctggccat gttcagaatc
     4981 ccatagaaaa tccacgtgac ggtgatttaa cccgttcagg ttcagttcgg ttctctctgc
     5041 tcactccgtc tctcatgtca aagcaatgct cccgcgcaac atcatgttgc agccttctga
     5101 ccagttcatg ttgcttaacc ggtgtatcca gccattccct gatgtcatgc ccgaccattc
     5161 gcgcctgttc aagatggcgg gaatagtccg gttcagaccg tttttgcatg atgtcatgac
     5221 gctgggcatc atcaagtcgt ccggcagctt caaaacccag atgcacttcg ggacgtcgtt
     5281 caatgccctg ttctttcagt gtccggcagt caatgaagcc cctttcaaag ccatgctttc
     5341 tcatatgttc gttgtgcttc acctcccagc gttttcgcaa ttcccgcaac tgctcttttt
     5401 gctgagtggg agtcaggctt ccggaatcct ttttcgcacc tccccgctcg ggatacctgg
     5461 cgttatagcg ccggaaatac tgttccggtg tacggtcaat gccatcattc acacgctgac
     5521 tcatcatgat atgcgcatga ggctgttcgc cgccgtcaat gctcgctttc ggattgtgaa
     5581 tggcaaaact ccaggcgtgc cgttcgccag cttcctgacg tacaaaatcc tgcaccagtt
     5641 caaggcgctg ttccggtgtc agttcacggg gtaatgcaat ttcaagttcc ctataggtcg
     5701 aaccgttcgc acgttcaaac tggtcagccg cctgccagaa atgggaagga ttatccctgg
     5761 cccattccgg catgtttccg tgtgcggaat attccaaatc ctgacggtcg cggtatttct
     5821 ctttgcgctc gatgtaatcg gcgtggtttg cagcctgccc cttgccacca aatttaacgc
     5881 tgaggtgata ggttgccacg gtttcagccc tccgcaggag cgcactgttt ctcgacgaag
     5941 gagagaaaca tataagtgcg cttccttcta attattcgaa ttagcacgga agataccccc
     6001 tgcacccgcc gaccgcaagc gatatactgc acgaccacaa accgccgtaa aaaaacacca
     6061 gggagtacca caaacatgcg catcagcaac gtcgaatggc tgaagaaacg gatcggattc
     6121 atcaggaagc tcggagaaca gaccgcgcga cagagacaga ttattgacct gctcgacaac
     6181 gaagccggac tcacagaaca ggaacgcaaa cttctccacg tgctggcaac agcggaaaaa
     6241 aacgacttac aggcacagga gaacgagcga aaacagacga tccagaaacg aatcgaggga
     6301 aaaaaacaac gacgggaacg caatcaccga ctctttctcg cggctgggct actgatcgaa
     6361 gcaggactgg tagacacaaa aacaggcgag ctatgctata aaaaagacag gatattacag
     6421 gaacttaagg aaattaagta cgatctggag acatccccaa acccagacgc gtaagtgcct
     6481 ccatccgtgt tccggttccc tggtgcttcc tcgctcattc gctcgctacg ctcggtcatg
     6541 agactgcggc gagcactacc agcacaatca tgcggaaaac agcaccattg cagggctgaa
     6601 cggggaaaaa cggcaaaaga cctgctgctc aaaagaacgc cagaaaggcg ctgttatctg
     6661 atataggcta ttttccgctt ttccagtttc gaatggttcg agtagaaacc tccaacatcg
     6721 ccgcaatagc tttctgagaa tatccattct ccagcataca tagcgccatg aaacgcttgt
     6781 catcgtaggc ttcgccttta gcgatgccac ctttcctgcc tctcttggcc tgtatttctg
     6841 gcgtgtgcgt atcagccaca tactgtgcga aagtttccgg cgtgaagttc ctgtgcgtgt
     6901 atttcgcaat actcttgcca atagcccgac attcaggagg tgaaagcgga acgggaagcg
     6961 atgcgttgta catttcgaca cgctgaatca cggcatcaag ccactgtgag aatgctggcc
     7021 agccctgacg aatcgccctg taagcccatt tgcgcgtcat ttcgaacagg tggcagtttc
     7081 gccccatccc gtaatgttta tcgatgctac gacgcgctga ggcgctcaaa tcgagataat
     7141 cagccagttc atcgagggta taggcttcct cgcgccactc catcaccagc cattccaggt
     7201 ggaacggatt tttgcaaatc aggccgctgt agttcacatc cgcacaaagt ttctcacaca
     7261 gcgaacgttc aaccgctgcg gcatatttca gcgccttaac cgaggcatca ggcgcggttc
     7321 tcacggcgat gttgagtgcg tagagcaggt gagcgtgtcc gttcacagga tttttaacgg
     7381 tgatgttcgg cgcgggagcg ttccggtcgc tccagtcaat cgcagccccc tcgcggtcaa
     7441 cgtcaaaaac cagccagaac tg
//
