LOCUS       NZ_CP049123             7867 bp    DNA     circular CON 16-JUN-2023
DEFINITION  Escherichia coli strain EC931 plasmid pEC931_3, complete sequence.
ACCESSION   NZ_CP049123
VERSION     NZ_CP049123.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14164122
            Assembly: GCF_014522225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7867)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of a tigecycline and colistin resistant
            Escherichia coli isolate EC931
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7867)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. 3.12; Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/15/2023 02:18:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,104
            CDSs (total)                      :: 4,985
            Genes (coding)                    :: 4,684
            CDSs (with protein)               :: 4,684
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 83 of 301
            Pseudo Genes (incomplete)         :: 207 of 301
            Pseudo Genes (internal stop)      :: 51 of 301
            Pseudo Genes (multiple problems)  :: 40 of 301
            Pseudo Genes (short protein)      :: 5 of 301
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049123.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC931"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC931_3"
                     /geo_loc_name="China: Yiwu"
                     /lat_lon="29.334975 N 120.121983 E"
                     /collection_date="2017-02-10"
                     /collected_by="Jianyong Wu"
     gene            join(6368..7867,1..72)
                     /locus_tag="G6G93_RS25005"
                     /old_locus_tag="G6G93_25090"
     CDS             join(6368..7867,1..72)
                     /locus_tag="G6G93_RS25005"
                     /old_locus_tag="G6G93_25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017782005.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_039026336.1"
                     /translation="MTHIACHINTLQLPFVACTVPLTFTLPAAGCTLIGRNGIGKSLLL
                     ELLAGQRRADSGKIEWLTPISWLSQFAPAQDHDCAASVADLIGWGEPLRALERIEQGSC
                     AQDDFELLADRWNLRSDLADLLAEAGLRLDPHVPVSTLSGGQLTRLRLLALFARPAHFL
                     LLDEPDNHLDASGIDWLSQQLARHRGGYLLVSHDRRLLNQTSHILELSEQGLQSARLSF
                     DEFLAQQAEVQAARSTSLAQAVRSEKAAARSLQKVREQAARRASQGRRERGSQAKILLD
                     FKAEQAGKSLGRVLDRHERGHSAQVEARTEAAAALAAVQPLIMQWPEEIERKGAAQPLV
                     ITAASHVRWHGQRVDLVLQRGERCLLGGRNGCGKSSLLQMLRGVLEPETGSFRINGKMA
                     CLDQGLSLIARDLSPLANLLTVAPTLAESDARTRLAGIALRGDRALTPCHGLSGGERLK
                     LGLLMVLAQLPDLLLLDEPDNHLDHPSRQLLTQVLADYPGTLLLVSHDPDFVAGVGVGK
                     ELNLSE"
                     /name="ATP-binding cassette domain-containing protein"
     gene            <157..447
                     /locus_tag="G6G93_RS25010"
                     /old_locus_tag="G6G93_25095"
                     /pseudo=""
     CDS             <157..447
                     /locus_tag="G6G93_RS25010"
                     /old_locus_tag="G6G93_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004661993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /name="type II restriction endonuclease"
     gene            complement(1263..1454)
                     /locus_tag="G6G93_RS25015"
                     /old_locus_tag="G6G93_25100"
     CDS             complement(1263..1454)
                     /locus_tag="G6G93_RS25015"
                     /old_locus_tag="G6G93_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1874..1987
                     /locus_tag="G6G93_RS25020"
                     /old_locus_tag="G6G93_25105"
     CDS             1874..1987
                     /locus_tag="G6G93_RS25020"
                     /old_locus_tag="G6G93_25105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058670733.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPERHDKAPLAAATGN"
                     /name="hypothetical protein"
     gene            2432..2857
                     /locus_tag="G6G93_RS25025"
                     /old_locus_tag="G6G93_25110"
     CDS             2432..2857
                     /locus_tag="G6G93_RS25025"
                     /old_locus_tag="G6G93_25110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676030.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="WP_004884531.1"
                     /translation="MGSYVDQSLTRNESVISRAQTSWIPTIIPVIIGILLLPFYGLGLL
                     IIIPVLLRVWSTELALTNQRVIAKVGFIRRNTVELRIDKVESLGIHQGILGRIFNYGSI
                     VIKGTGGTNTPIPNIKAPMQFRSIVNNHIEEMNSKQQ"
                     /name="PH domain-containing protein"
     gene            4052..4195
                     /locus_tag="G6G93_RS25030"
                     /old_locus_tag="G6G93_25115"
     CDS             4052..4195
                     /locus_tag="G6G93_RS25030"
                     /old_locus_tag="G6G93_25115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_079849604.1"
                     /translation="MHEPPVQSDRCALSGNYHLESNPERHDKAPLAAATGNGFAEQRSV
                     EV"
                     /name="replication initiation protein"
     gene            4430..5467
                     /locus_tag="G6G93_RS25035"
                     /old_locus_tag="G6G93_25120"
     CDS             4430..5467
                     /locus_tag="G6G93_RS25035"
                     /old_locus_tag="G6G93_25120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RnlA family toxin"
                     /protein_id="WP_085004987.1"
                     /translation="MAQNPFKALNINTDKIEAALTQNGVTNFSSIVRNERETHLSGTYK
                     EIDFLIKLMPSGGNTTIGRASGQNNTYFDEIAGIIKENCLYSDKKTFEYTIPRFSDENK
                     EMLFAFLTEENVKIEEDKNNDPNCKHQYIMTTGNGDRVRAKIYNRGSIQFQGKYLQIAS
                     LINDFMCTILDMKEIIEQKNQEFNVDIKKETIESELHSKLPKSIDLIHDDIKKQLSCSL
                     IMKQIDVEMEDYSTYCFSALRSIEGFIYQLLGSVCNPSSSRNLGEYFIENNPKYIIRDI
                     HQETINGEIAEVLCECYTYWHENRHGLFHMKPGVADTKTIDKAESIEIIDTVCQLIDKG
                     MARLK"
                     /name="type II toxin-antitoxin system RnlA family toxin"
     gene            5467..5847
                     /locus_tag="G6G93_RS25040"
                     /old_locus_tag="G6G93_25125"
     CDS             5467..5847
                     /locus_tag="G6G93_RS25040"
                     /old_locus_tag="G6G93_25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RnlB family
                     antitoxin"
                     /protein_id="WP_079849606.1"
                     /translation="MKINKKYQIEVIKDKDKTLFIVYATDIYSPSEFFSKIESDLKKKK
                     VGGDVFFDLIIPNGGKKDRYVHTSFNGERLTSYTLNKVTETDKYKSLSERSASFFKNNF
                     NNINANLLSKATSFALKQGIPI"
                     /name="type II toxin-antitoxin system RnlB family
                     antitoxin"
     Typing          1634..1987
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.191"
                     /coverage="95.0"
                     /evalue="1.27e-168"
     Typing          3674..4162
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.495"
                     /coverage="95.0"
                     /evalue="0.0"
ORIGIN
        1 ctattggttt ctcacgatcc cgacttcgtt gcaggggttg gggttggtaa agaattaaat
       61 ctttcagagt gaccgcaaga tgattgacct agatgccctt gtggcagggg gttagaggag
      121 ttgacggaaa tttcggcggt tccggcttac aaccccgaat accataaccc ggcgttcgac
      181 tcgctgaagc tgaccatgct tggtgtgaaa tcgacctgca aggaccgctg gcggcaggtt
      241 ctcgccgaag cagaccgcat tgatgacaag catctgctga cgctggaaac agcaatctcc
      301 acgcatcaga ccgacgaaat ggcagcaaag cggctgcaac tcgttctgcc gcgcagtctg
      361 caccagacct atacgccagc ccagcaggca tggttgatgg atgttgtcag tttcacggaa
      421 ctggtacgtg ctcgacagaa tgcgtgagtg ctgaacttcg gcttagcgta cgattttttc
      481 cgttttctga gccgacccct ttctgaggcc tccggcctga aacacacacc tttctgtgcc
      541 catatcgaga aaaaacgccc ctgatgattc tggcgcgact gcatggctat gcacggacat
      601 acctcgccgg gattttagcg gctaaaatcc gatgaacgcc ctgaaagcca ccacgaacca
      661 ccccgataac ggtgaacgga tgcgcaattt attgcgtggc cgtgaggcgt tgccggcaca
      721 gccggaaaaa ccattttcag cacatgcagg aggcccaaga agagggggcg gatgtacgaa
      781 tagttcagag gtcagatctg acgtaaaaat gatgtaaaag tgacgtaaaa gtgatgtact
      841 tttgatgtaa ttctgttgta cacccgcgat ctgacgtaaa agtgatgtaa ttttgatgta
      901 cttttgatgt aattctgttg tacacccgcg ttctggggta aaagcagatg caattttgta
      961 gtttatgtgt agtcgctgaa cccatagggt catacgagca tgaccctttc ccggaaaagg
     1021 acgcttttta gccagaatca tccttaacct gcggcgccag cactcgggag tggtcgtttt
     1081 atatactaaa aaatgacata cacctaacgc agctgcgatt ttcatcgcaa cttcatgaaa
     1141 aataaataaa aaagaaaaaa cacagggatt ctgggtatta ccggggtaat aatgttaagc
     1201 gctaacacaa tgaaaattaa ggcgatgccg gtattactcc ggtaataccc agaacggagc
     1261 cattacgcac cgtcctgctg ctcattcagg cgcgctgaca ggcttcggta aaggcgttcc
     1321 gcatcctcat ggagtttctc gcagaaatca gcctcatgat ccagatccag ctcattaagc
     1381 ctgtccagaa gaagcaggga ctgatcctgg atgaatttag ccatgttaac ggcagtcttt
     1441 tgttgtttgt tcatgaaagc cccggcgcga aagtgcttac tgtacgttcg gatggttttc
     1501 cgcttcctcg ctcattcgct cactccgttc ggtcatgagt ctgcggcgag cggagggagg
     1561 ataagcagat aacgtaacgg taagcataac gaaagctaag gacaagggcg aaaaggccac
     1621 gccccgccgg ggcgtttttc cataggctcc gcccccctga caagcatcac gaaaatctga
     1681 cgctcaaatc agtggtggcg aaacccgaca ggactataaa gatcccaggc gtttccccct
     1741 ggtagctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg
     1801 ttatggccgc gtttatctca ttccacgcct gacactcagt tccgggtagg cagttcgctc
     1861 caagctggac tgtatgcacg aaccccccgt tcagtccgac tgctgcgcct tgtccggtaa
     1921 ctatcatctt gagtccaacc cggaaagaca cgacaaagcg ccactggcag cagccactgg
     1981 taactgattt agaggagtta gtcttgaagt tatgcgccgg ttaaggctaa actgaaagga
     2041 cagatttggt tactgcgctc ctccaagcca gttaccgcgg ttcaaagagt tggtagctct
     2101 gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg tttccagaga aagagattac
     2161 gacgcaacgg acaggatctc aagaagatca tctgtgatat tcgacagata tgggataaag
     2221 gattgcaacg ccaagagtat gtgagacata gcagcagcca ccaccaccac agaatcaagc
     2281 cagttgcgtc acgaaaggaa agaaccgaac tgtgcgacgg tcgctacgct ctccgtaaat
     2341 tttgtatacc caccacggag acataaagtt tctattgctg attcgcctta aaaagggaaa
     2401 aatcctattt tttctaaatg gatataacct tatgggaagc tacgtagacc aatcattaac
     2461 gagaaatgaa tccgtaatat ctcgcgctca aacctcctgg attccaacaa tcatcccagt
     2521 aattatcgga atcttgttgc tgccttttta cggcttaggg ttacttataa taatccctgt
     2581 gctccttaga gtttggtcaa cggagcttgc attaactaac caaagagtca tcgcaaaagt
     2641 gggttttatc cgacgcaata cggtggaact ccgtattgat aaagttgaaa gccttggcat
     2701 acatcaaggg atactgggac gaatctttaa ctatgggtct atcgtgatta aaggcactgg
     2761 cggaaccaac actccaattc caaacatcaa ggcgccaatg caattcagat cgattgtgaa
     2821 caatcatatc gaggaaatga attctaagca gcagtagctt cctcgtagca ttacaaaaaa
     2881 agccctgcgg ggcttttttt gtacctgcca atcagctgga tttaccataa catccgttgc
     2941 ccgcaccgcc tgagaggccc tcagggcgtt tctggggcct cctgcctgaa aacggggatt
     3001 aacccccgtt aaaaaccggt tttaagccac agttaatttc tgcgcgtgtg catagccatg
     3061 cacacgaacg catggccgcc attttagcgg ctaaaatccc ctgtacgccc tgaaagcaat
     3121 catgaaccac cctgataacg gtgaacgggt gcgcagttta ctgtgcggcc gtgaggcgtt
     3181 ggccaggccg tcaggcatgg aaggatttat tgctaagggg gtcggattta acgtggatga
     3241 atcagcacgc agtgatgaac gggattcgga aggggcaccc ttgcggcgcg gcgatgtata
     3301 catcgctaac gtgccttggt catttattgc aagaagggag aggtagcatt ttgatagcaa
     3361 aaaagtagca aaaagatagc aaccgaaaga ccagaagact ctggagatag caaaaaagta
     3421 gcaaaatggt agcaaaaaga tagcaaacat gcgggagcta accaggagtc tttagcgggc
     3481 cggcggtact ccaaaaacac atcttgtccc gctgttttgt gaataagcca gcatggctgt
     3541 gggcacaaaa catttcctgt ccgctgcttt aacctttgta atccaggaac gtcaggtgac
     3601 gtcactttgc ggcgttcgcc ctatcctgca tcgtattaaa aaatgcagct atggcgcgta
     3661 cggtgccaaa gagaagcccg cagatattcc gcttcctcgc tcactgactc gctccgctcg
     3721 gtcgttcggc tgcggcgagc gttacctgca cacggataag gacaaaggca tacggcgtga
     3781 caggcagggc agaaaaagga tgcggcgctg ccgtttttcc ataggctccg cccccctgac
     3841 aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga
     3901 taccaggcgt ttccccctgg tggctccctc gtgcgctctc ctgttcctgc ctttcggttt
     3961 accggtgtca ctccgctgtt atggccgcgt ttatctcatt ccacgcctga cactcagttc
     4021 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
     4081 ctgcgccttg tccggtaact atcatcttga gtccaacccg gaaaggcacg acaaagcgcc
     4141 actggcagca gccactggta acggattcgc agaacaaaga agtgttgaag tgtgaggtga
     4201 agtacagcta cactgtaata acgaatttgg tttctgtgct ctgcgaaagc cagttacctc
     4261 ggttcaaaga gttggtagct caacgaacct tgagaaaacc accgttggta gcggtggttt
     4321 tttctttatg cgatgatgaa tcaatcagtc tatcaagtca acgaaccgcg cttccattga
     4381 caaaaaaaca agatctgcac atattcagaa aaaacaacgg attgaggcaa tggcacagaa
     4441 cccatttaaa gcactaaata ttaatacaga caagattgaa gctgccttga cgcagaatgg
     4501 cgtcacaaac ttttcttcta tcgtgagaaa cgaaagagaa acacatttat cagggacata
     4561 taaagaaata gattttttaa taaagctaat gccatcaggt ggaaacacca ctataggaag
     4621 ggcatcagga caaaacaaca cttactttga tgagatcgct ggcattataa aagaaaactg
     4681 tttgtattca gataaaaaaa cgtttgaata cacaatccca agattcagcg atgaaaataa
     4741 ggaaatgttg tttgcatttc ttactgaaga aaacgtaaaa atagaagaag ataaaaacaa
     4801 cgaccccaac tgtaaacatc aatacatcat gaccactggc aatggtgaca gagtcagagc
     4861 aaaaatttac aaccgtggct caattcaatt ccaaggtaaa tatcttcaaa tcgcaagttt
     4921 gattaatgat ttcatgtgca ccatactgga catgaaagag attatagaac agaagaatca
     4981 agaatttaat gttgacataa aaaaagaaac aattgagtca gaattacata gtaaactacc
     5041 aaaaagcatc gacctaatcc atgacgacat caaaaaacag ctatcctgct cgctcataat
     5101 gaaacaaata gatgtcgaaa tggaagatta ctcaacatat tgtttctcag ccttgagatc
     5161 tatagaaggc tttatatatc aactacttgg aagcgtatgc aacccatcat catcaaggaa
     5221 tcttggcgag tacttcattg agaataaccc gaaatacata ataagagata tacaccaaga
     5281 aactattaat ggtgaaatag cagaagtttt gtgtgagtgc tacacttact ggcatgaaaa
     5341 cagacatggc ttgttccata tgaaaccagg tgtggctgac actaagacaa ttgacaaagc
     5401 agaatctatt gaaattatcg atactgtatg tcagttgata gataaaggca tggctaggtt
     5461 gaaataatga aaataaacaa aaaatatcaa atagaggtga taaaggataa agataaaact
     5521 ttattcattg tctacgctac agatatttat agcccgagcg aatttttctc taaaatcgag
     5581 tctgatttaa agaaaaagaa agttggtggc gatgtttttt ttgatttaat aattcctaat
     5641 ggtggaaaaa aagaccgtta cgtccataca tcattcaacg gcgagcgatt gacaagctac
     5701 acattaaaca aagttacgga aacagataag tataaatcct tatcggaacg ctcagcttcg
     5761 ttctttaaga ataactttaa taatattaat gcaaaccttc tatctaaagc cacatcattt
     5821 gctttaaaac aaggtattcc aatataaaag caagctcaga tattgaacat aacatccgtt
     5881 gcccgcaccg cctcagaggc cctcagggcg tttctgggga cggtagagaa aacggacaaa
     5941 atcgtacgct aagcatgcgg cctgtcaggt tgaggccata gagtttgacg tttaggcgtc
     6001 agataatgca gaaagcggcc ctagtctgac gcgatgccag tgagccgact gacgcccagt
     6061 tgaggtatag cccagactga cacttgactt attgatacaa catgcaagtg agtgataccg
     6121 gaacatttgg cattttttac tatctgaagt atgattttac tttcatcagt aaagagtatt
     6181 tcgccgatgc gcatttaacc tgtccgcacc ccacttttga ttcttctccc gccagggaga
     6241 gcgggtgcgt ctcaccgtga caggttgatg ctgattcctc tgctgttttc ccactagtgg
     6301 acacgtgtcc ctgtcatttg gtttagctct tggcctgcgg cttccgcatt ggcctacagg
     6361 agttcagatg acccatatcg catgtcatat caacactttg cagctgccct tcgtcgcctg
     6421 tactgtgccc cttaccttca ctttgcccgc tgcgggctgc accctgatcg gccgtaacgg
     6481 catcggcaag agcctgctgc tggaactgct ggcgggccag cgccgcgcgg attctggcaa
     6541 gatcgagtgg ttgaccccca tcagctggtt gtcccagttt gcaccggctc aagatcatga
     6601 ctgcgcagcc agtgtggccg accttatcgg ctggggcgag ccgctacggg cccttgagcg
     6661 catcgagcag gggagctgcg cccaggacga tttcgagttg ctggctgatc gctggaacct
     6721 gcgcagcgat cttgccgacc tgctggcaga agcaggcctc agactcgatc cccatgtccc
     6781 ggtcagcacc ctgagcggtg gccagctcac ccgtttgcgc ctgctggcgc tgtttgcgag
     6841 gcctgctcac tttctgttgc ttgatgagcc agacaaccac ctcgatgcct ctggcatcga
     6901 ctggctaagc cagcaactgg ccagacatcg cggaggttac ctgctggtga gccacgaccg
     6961 gcgcctgctc aatcaaacca gtcatattct ggagctgagc gagcaggggt tgcagagtgc
     7021 ccgcctctcg tttgacgagt ttctggcaca gcaggccgag gtacaggctg cccgcagcac
     7081 cagtttggcg caagcggtgc gaagcgaaaa ggcggcggcg cgcagcttgc aaaaagtgcg
     7141 tgaacaggcg gccagacgag ccagtcaggg gcggcgtgag cgcggctccc aagccaaaat
     7201 cctgcttgat ttcaaggcgg aacaggcagg caaatccctt ggcagagtgc tggaccgcca
     7261 cgagcggggc cacagtgcgc aagtggaggc ccgcaccgag gcagcagccg cgctggcggc
     7321 ggtgcagccg ctcatcatgc agtggccgga ggagatcgag cgcaaggggg ctgcccagcc
     7381 gctggtgatt acggctgcgt ctcacgtgcg ctggcatggc cagcgggttg atctggtgct
     7441 gcaacggggt gagcgctgtt tgctcggcgg tcgcaatggc tgcggcaaat cgagtctgct
     7501 gcagatgttg cgtggggtgc tggagcccga gacgggcagc tttcgcatca atggcaagat
     7561 ggcttgcctc gatcaggggc tcagcctgat tgcgcgggat ctcagcccgc ttgccaacct
     7621 gttgacagtg gctccgacac tggccgagag tgatgcgaga acacggctgg cgggcatagc
     7681 cctgcgcggc gatcgtgctc tgacgccttg tcatggtctg agtggggggg agaggttaaa
     7741 actgggtctg ctgatggtac tggcgcaact gcccgacctg ctgctgctcg atgaaccgga
     7801 caaccatctg gatcacccct cgcgtcagtt gctcactcag gtcctggcgg actacccagg
     7861 cactctg
//
