LOCUS       NZ_CP049106             4073 bp    DNA     circular CON 12-JUN-2023
DEFINITION  Escherichia coli strain EC28 plasmid p6, complete sequence.
ACCESSION   NZ_CP049106
VERSION     NZ_CP049106.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14149865
            Assembly: GCF_011044355.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   He,F.
  TITLE     Genomic and phylogenetic analysis ESBL-producing Escherichia coli
            ST429 strain isolated from a urinary tract infection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   He,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2020) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, Hangzhou, Zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/12/2023 02:51:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,169
            CDSs (total)                      :: 5,056
            Genes (coding)                    :: 4,878
            CDSs (with protein)               :: 4,878
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 65 of 178
            Pseudo Genes (incomplete)         :: 121 of 178
            Pseudo Genes (internal stop)      :: 32 of 178
            Pseudo Genes (multiple problems)  :: 36 of 178
            Pseudo Genes (short protein)      :: 4 of 178
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049106.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC28"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p6"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2018-06-03"
                     /collected_by="Fang He"
     gene            complement(join(4056..4073,1..2130))
                     /gene="mobQ"
                     /locus_tag="G5V60_RS25665"
                     /old_locus_tag="G5V60_25630"
     CDS             complement(join(4056..4073,1..2130))
                     /gene="mobQ"
                     /locus_tag="G5V60_RS25665"
                     /old_locus_tag="G5V60_25630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029354.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            2309..2563
                     /gene="mobC"
                     /locus_tag="G5V60_RS25670"
                     /old_locus_tag="G5V60_25635"
     CDS             2309..2563
                     /gene="mobC"
                     /locus_tag="G5V60_RS25670"
                     /old_locus_tag="G5V60_25635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            3097..3798
                     /locus_tag="G5V60_RS25675"
                     /old_locus_tag="G5V60_25640"
     CDS             3097..3798
                     /locus_tag="G5V60_RS25675"
                     /old_locus_tag="G5V60_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(1773..2131)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2132..2308
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          3098..3798
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2..2217)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.962"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atcatgtctc ctctcggtgg tgtgtgttcg tgcatcttcc tcgcgctgcc attcctccca
       61 ggtacgatta aaacccagcg agcggaacag atgatccggc tgtttttctg gtgtgttgac
      121 ggcagcaaaa agcggtgcct cacgggtttt cagatgaaac tgcatcagtt ctggtgcagg
      181 tgatatgtgg ctgtctgcat cgtgatttac ggcaacacca taccctgcat accgtaaccc
      241 cttatcggtc agcgcactga tgaccagatg cctgttatcg gctgagggaa cggcaaacgc
      301 gggatacagc gtaccattaa gccggataac accgcccaga gagtcatcca cgcccatgac
      361 cagcaaaagc tgaagctggc gggtttcccg gtcgacagaa ctcatgaccg gcatcagcgt
      421 cggtggcaga cgtccctgca taaaatccac cttccttgcc gctggtgatg gcatggctgg
      481 ctggcggtct tctccaagac agaaagactc cggccatgtc ttcggctggc ggagattgcg
      541 ggtatgttgc ggccatgtct ggcaaacctg cgtcagcagt tgctcaaagg ttgcagagga
      601 ataaacacgt tgcagtggcg gacgttggcc gatttcgctt cggtcacaga tataaccact
      661 cctgtcacgc attaaatcaa cactctcaaa gattctggtg cggtatttct ccagctcgtt
      721 cccccagagc gtatattctc cgttccggtt gtgaagcaga aggtaacagg tcatttcccc
      781 gttaccaggc atgggagcct cgccataccc cgtaacgact gctctcagca cccctgaatc
      841 cataacggca ttgtcatttt tcctgagctg ctccgcgcga ctacgttcag tatcttcctg
      901 ttccttccgg cgcaattcct cttcacgttt aagcaactgg gctctgagag ctgcaaggcc
      961 ggattccccc cactgctgcc actgtttttc aaatgccatc tgactcgcgg gaatgtcctg
     1021 tttctccacc ttccggtaat gctgtcgtaa tttttccatt tgctggctca gagactggat
     1081 ttttccctgc tcactacggt attccggtgc cgcccattgc atcaggggaa tacgggaaaa
     1141 accggacgaa aacggcgatg attttttcag gctggcagca tggctcatcg cttcctttcg
     1201 ttcggtgttc agctcttccc agcgtctgcg cgtcgcctgc atatctgcca tcatcgacat
     1261 ggcattactg gtgagtttcc atgccgagtt actgtccttc ggcaacgctt cgataatgtc
     1321 acgtagtgtg tacgaagttg cgttgtcagg aagcccgata ttcagaaggt cagccacacg
     1381 gcgggcaggc tctgtcagcc ctccgttcct gagttcggtg cgggaaatct gtatttccag
     1441 gttaagcctg ttaatcagac gattctgttc gccgcgttct gtttcgatac cgcgtgtttc
     1501 catcgcacag gcagactttc cgaggtgaat accaggcgtt ttctcaagcc cctgtcgctc
     1561 gtatgaacga tggtctatct cttcctgata accggcgttc gccagtgccc tgtttgcatg
     1621 gtcagcccat gatgcccgcc acgtctcggc atgtgtccgg tcgttccagt ccctgacctt
     1681 tccgctgaac ccagcaggtc cgatagccct cgtggtcagc atgatgtgcg catggggatt
     1741 tgttttatcc atgtgatgga acgccacatc agcaatcatg ccctgactga caaagttttc
     1801 tctgacaaag gcgagaacgg tttcccgtgc ggcatcctgc ggcagttcgt gggggatggc
     1861 gagttcaatt tcccgcgcaa gttgtgagtt cttccggcgt tcggccttct caacggcatt
     1921 ccagagttcg gcacggttcg tacaccaggc aggggcggtg gcgggggtca gaatcacagc
     1981 ctcagccaca ccgcttttgc gggtgtagtc gtgagtcagt ccggtacgct catcaagcat
     2041 cagtgaaccg gaacgatagg cagcggaagc tacggcgctg tagccgctgc ttctggagat
     2101 aattttcatg ctgagatgat aaatcgccat ttctgccgta atgccttttt gtagttttct
     2161 ttcagccccg cagggcgcac acaatcgaaa cggagtggag attgtataag tgcgcacttc
     2221 gtgatttgcc gtgcattatg ccagctcagt ttttaaacta ccagtgacgc gctaaaatgc
     2281 gatttttcat cgcgcacgga gttacatcat gagcagaacg ctcgaacaga agattgctga
     2341 tgctgaggcg agattacagc gcctgaaggc gaagagccgg agcctggaca cagcgcagaa
     2401 ggttgttgtg ggtgctgcat tgctggcaaa ggtcagaaag ccggaggagg tgcagttgcg
     2461 tgcctggtta ctccagttcc tgaaggcaga ggtgacacga caggctgatg tgacgcgcat
     2521 actgccgctg attaacgagc tggaggcgct gccagaacag tgattgtatc gtccgctcgc
     2581 cgcagcccca gcgaccgagc gttagcgagc gagtgggcga ggaagcggaa gtgaatcagt
     2641 cgccaggttg agcacatacg cacggttttt cggcaggtga tatgcgctga acccgcatga
     2701 atactgggtt tccacagggt tggagggggt gtattcgaaa cttgcaattc tgctttattg
     2761 caacgcattg tttttattgg ttttttgttt tcagcacccc aagttatcca caggctcgcc
     2821 gttaagtttt taagatctta tttttagatc ttttttttta aaaccttttt aggtcttgta
     2881 acaccatgat tttaaagatg ttctggcaag ataagtggag cttttggggg tgaaaggtgg
     2941 agcttttggg ggtgaaaggt ggagcttttg ggggtgaaag gtggagcttt tgggggtgta
     3001 atccacagaa aaagggggtt gcaaaaaaaa aacttccacc catatgataa gtggcgtaca
     3061 gtaaaaaaac gccaccaata gcaacgagca agcgaaatgt cagatctgat agcttacaaa
     3121 tcaaacgccc ttatcgaggc aagctacaag ctcactttgc aggaacagcg cttcctgctg
     3181 gtctgtatca gccgcctaaa atccggagaa gattcccctt cgccggatga acaaaaaacc
     3241 atgaccatca cggctgcaga gtttcatgat gcctttcctg acatgggacg gcagcacgct
     3301 gaagctaggc tcaaggaagc gatagatcgc ctctgggaca ggtcagtaat catcaagaac
     3361 gaggaaaaac gagaagagtt ccgctgggtg cagtaccggg cgcagtattt caaaggtgag
     3421 gcgaaagtag atatcacctt ctccgatgcc atcatgccgt atctgacaca gctcaagggg
     3481 cagtttacgc gggtagtcgt gaagaacgtc tccagccttt ccagcaccta cagcatccgc
     3541 atttacgagc tgctccagca gttccgcagc accggtgaca gaacgatagc tatcgatgat
     3601 ttccgaacca tgcttgccgt tgaagataaa tatcaacaat tcaaagactt gaataagatg
     3661 ataatcaagc cagccatcgc cgaactgaac gaaaaaagcg atctggttgt aactgttgag
     3721 acgatcaaaa aggggcgcac ggttgtagcc ctgcattttc gcttcaagga agacaaacag
     3781 atcaagatga cgatctaaaa tcgattttaa ggcacctaaa acgctccctg ctgcgttttt
     3841 tttgtgttag tagtgcgaag gtactattga agtgaaaaat tgcgtcaaaa acgctctgat
     3901 gcagcgaaaa aagacccccc aatttttggg ggcggaaaaa caaaactatg cttgcgttat
     3961 gtgagtgtgc ttcttgcatt catccctgtc gatgaacatc gaagaacatc gacgaacata
     4021 acacgatgtt aaacaaaaca aaatattgtt cacattcact gtgaccaacc gcg
//
