LOCUS       NZ_CP049083             6800 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain p10A plasmid p10A_p2, complete sequence.
ACCESSION   NZ_CP049083
VERSION     NZ_CP049083.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13948681
            Assembly: GCF_011331065.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Shropshire,W.C.
  TITLE     Concurrence of Porin Loss and Modular Amplification of
            Beta-Lactamase Encoding Genes Drives Carbapenem Resistance in
            Recurrent Enterobacterales Bacteremia Isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6800)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2020) Epidemiology, Human Genetics and
            Environmental Sciences, The University of Texas Health Science
            Center at Houston, 1200 Pressler Street, Houston, Texas 77030,
            United States of America
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.6
            Assembly Name                     :: 10A
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 373x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011331065.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:39:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,856
            CDSs (total)                      :: 4,733
            Genes (coding)                    :: 4,487
            CDSs (with protein)               :: 4,487
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 76 of 246
            Pseudo Genes (incomplete)         :: 182 of 246
            Pseudo Genes (internal stop)      :: 35 of 246
            Pseudo Genes (multiple problems)  :: 44 of 246
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049083.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="p10A"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p10A_p2"
                     /geo_loc_name="USA:Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2015-01/2016-07"
                     /collected_by="MDACC"
                     /genotype="ST10"
     gene            1..390
                     /locus_tag="G6P90_RS24030"
                     /old_locus_tag="G6P90_24030"
     CDS             1..390
                     /locus_tag="G6P90_RS24030"
                     /old_locus_tag="G6P90_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            434..>838
                     /locus_tag="G6P90_RS23865"
                     /old_locus_tag="G6P90_23860"
                     /pseudo=""
     CDS             434..>838
                     /locus_tag="G6P90_RS23865"
                     /old_locus_tag="G6P90_23860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(945..1781)
                     /locus_tag="G6P90_RS23870"
                     /old_locus_tag="G6P90_23865"
     CDS             complement(945..1781)
                     /locus_tag="G6P90_RS23870"
                     /old_locus_tag="G6P90_23865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(1781..>2056)
                     /locus_tag="G6P90_RS23875"
                     /old_locus_tag="G6P90_23870"
                     /pseudo=""
     CDS             complement(1781..>2056)
                     /locus_tag="G6P90_RS23875"
                     /old_locus_tag="G6P90_23870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
                     /name="aminoglycoside phosphotransferase APH(3')"
     gene            complement(2131..2604)
                     /gene="dfrA14"
                     /locus_tag="G6P90_RS23880"
                     /old_locus_tag="G6P90_23875"
     ARG             complement(2135..2604)
                     /name="dfrA14"
                     /gene="dfrA14"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<2625..3152)
                     /locus_tag="G6P90_RS23885"
                     /old_locus_tag="G6P90_23880"
                     /pseudo=""
     CDS             complement(<2625..3152)
                     /locus_tag="G6P90_RS23885"
                     /old_locus_tag="G6P90_23880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /name="phosphotransferase"
     gene            complement(3213..4028)
                     /gene="sul2"
                     /locus_tag="G6P90_RS23890"
                     /old_locus_tag="G6P90_23885"
     ARG             complement(3217..4028)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(4488..4646)
                     /locus_tag="G6P90_RS24610"
     CDS             complement(4488..4646)
                     /locus_tag="G6P90_RS24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            5217..5366
                     /locus_tag="G6P90_RS23905"
                     /old_locus_tag="G6P90_24035"
     CDS             5217..5366
                     /locus_tag="G6P90_RS23905"
                     /old_locus_tag="G6P90_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(6143..6583)
                     /locus_tag="G6P90_RS23910"
                     /old_locus_tag="G6P90_23895"
     CDS             complement(6143..6583)
                     /locus_tag="G6P90_RS23910"
                     /old_locus_tag="G6P90_23895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     ARG             complement(949..1781)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(2611..3152)
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.43"
                     /coverage="67.42"
     ARG             complement(1782..2026)
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="30.34"
     Typing          5009..5182
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(4480..5085)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atgagtacag agttaaatag cggtactgtc ggtggttttg gtcacatggg accaaatgac
       61 ccctacaatg atggtcatag cggctctgga agttcgggag gtagttttaa cggtggtggt
      121 aatggtggcc atgataatag tagtggttca tcttatactg catcctttgg tctcatcact
      181 ggccgacacc cgataactgt cccaggtacg aagaatatta attctgtcga tctttctgct
      241 gtcattaata acattataca tcatgaaggt gcacgcaata acatgtacct agatactgcg
      301 aacaatgtaa gagtgggagt gacgggcact ggctggcaat gtctagcaac ggcaggcatt
      361 tcggctgagg gtaaaagaac tttccgctaa gcgatagact gtatgtaaac acagtattgc
      421 aaggacgcgg aacatgcctc atgtggcggc caggacggcc agccgggatc gggatactgg
      481 tcgttaccag agccaccgac ccgagcaaac ccttctctat cagatcgttg acgagtatta
      541 cccggcattc gctgcgctta tggcagagca gggaaaggaa ttgccgggct atgtgcaacg
      601 ggaatttgaa gaatttctcc aatgcgggcg gctggagcat ggctttctac gggttcgctg
      661 cgagtcttgc cacgccgagc acctggtcgc tttcagctgt aagcgtcgcg gtttctgccc
      721 gagctgtggg gcgcggcgga tggccgaaag tgccgccttg ctggttgatg aagtactgcc
      781 tgaacaaccc atgcgtcagt gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg
      841 ggtcgtttgc gggaaggggc ggaatcctac gctaaggctt tggccagcga tattctccgg
      901 tgagattgat gtgttcccag gggataggag aagtcgcttg atatctagta tgacgtctgt
      961 cgcacctgct tgatcgcggc cgcgatagct agatcgcgtt gctcctcttc tccatccgcg
     1021 ttccaagctg cggaaaggca cccataagcg tacgcctggt cgagcaggcg acgcggatcg
     1081 acgtccagcg cacgagagaa tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa
     1141 aggtcgtctc tgtcagccgg atcgtagaac atattggcgg cgccaaagcc cacttcaccg
     1201 accagaccga cgggatctat caccagccag ccgcgactgg agaacatgat gttttcatga
     1261 tgcagatcgc catgtagccc acgcagttcc gaggcattgc tcatcatttg atcggctata
     1321 atcgccgcgt ggacgtagtc agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga
     1381 aacaaagctg caaagcgatc ccggatcggg agaagggcag aaggcagggg ttcctcagat
     1441 gcggcataca gcttcgccat tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc
     1501 tcggcaacga tgtgagagag cattcgctcc ccggcatatt cgagcaacat cagattgttc
     1561 tcacgaccga gcaaccggac tgctcccctc ccattgcgcc ataccagata gtcggccccg
     1621 cgcagttcat cagcaatgtc ttctataggt ttcaatccct tgacgattgc aggagtcccg
     1681 tctggcaatg aaactttcca aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc
     1741 gaaacgtgcc aatgagcagg aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt
     1801 ccaatcgcag atagaaggca aggcgttcgc ggtcgggggc ttcgatcccc aatacattga
     1861 ataggacagc gaaggcgcgc tctgcttcat ctggcgctgc ccagttctct tcggcgttag
     1921 caatcatgag tgccaaatcg gcatagcgat ctgctgttcc gagccgccca aggtcgatca
     1981 gacccgtgca ttgaagagtt ttagggtcca ccatgaagtt cggcatgcag ggatcaccat
     2041 ggcaaacaac catatcgtcg ctatcaatcg cggaaaaagg cgtagtgcgc gaagcgaacg
     2101 aagctttttg ccgtcgattg catagctttg ttaacccttt ttccaaattt gatagcaata
     2161 gttaatgttt gaagtaaagt gttgctcaaa aacaacttcg aaggtatttg gaatactcgg
     2221 gaagaaaaca tccccctctg gctcgatgtc gatcgtcgat aagtggagcg tagaggccat
     2281 gggtaatgtt tctcggtaaa tttctccgcc accagacact ataacgtgac cggtgaattc
     2341 agctagcctg tccatggcct cttcgattga ctgaaataca actacattgt catcatttga
     2401 tgtccaacct gagcgggtaa cgaccgcgta tttcctattg gggagtgcgc ccatagattc
     2461 aaacgtcttg cgacccacca gaagccactg attgtaggtc aatgctttaa aaagtagctg
     2521 ctcccctttc gcggaccagg gtatgtctgg accgcaacca atcacgccgt ttttcgcttt
     2581 cgcagctatc aatgatactt tcaaggttct catcctgggt taacggtgcg ctcttggtcg
     2641 agccgcaccg gtagctctcg ttcgacacga gccaaaagat cgagctgcgg cgtactcttg
     2701 tcctcgtccg gtaagaagtc gggattgacg gcattgcggg acaccacatc aacggcgcgt
     2761 ccgaacattc gcgacagcct gcgctcaaac ggacattgat caaccgatag gctgtgaaca
     2821 gcgccaagtt gctgccccat tgacggccac gctttgagca aatccgctcc agacagatca
     2881 gccgccggta ctcccggaat tgccgttatc accaagcatg caccctcctg ttcctcctgc
     2941 cagttgatga cctcggggca agccacacct cgacctttga gccaaatgag gcggtcacgc
     3001 tctccagcga gctcaccgcg gcgggaagca ggtgcgattt tcgcgaaggc atgcccgtca
     3061 ccacgtcgaa aaacaaaatc accagattct ccgcctctga caggcaacca gtcagaatgc
     3121 gattcaccaa aaaaaatatt agttcgattc aatggaggtt ccttcagttt tctgatgaag
     3181 cgcgaatata gagaaatatc ccgaatgtgc agttaacgaa ttcttgcggt ttctttcagc
     3241 gccgccaata ccgccagccc gtcgcgcaag gggcgcggct cgtgtgtgcg gatgaagtca
     3301 gctccacctg cggcggcggc aagctctgca gcgagtgtcg cggccccgac atcccccgga
     3361 ccacggcctg tgagcgcgcg cagaaaggat ttgcgcgaaa cagacagaag caccggcaaa
     3421 tcgaagcgca gccgcaattc atcgaaccgc gccagcaccg agagcgaggt ttcgggagca
     3481 gcccccagaa aaaaccccat gccgggatca aggacaaggc ggttgcgttt gataccggca
     3541 cccgtcagcg ccgcgatgcg cgcgtcaaag aacgccgcaa tgtgatccat gatgtcgcca
     3601 gcgggtgcct cgcgccgatc tgcctgcccg tcttgcaccg aatgcataac gacgagtttg
     3661 gcagatgatt tcgccaattg cggatagaac gcagcgtctg gaaaaccgcg aatatcattg
     3721 agataggcca caccacgcga caaggcatag gcttgcgtcg cgggttgata actgtcgagc
     3781 gagacgggaa tgccatctgc cttgagcgcg tccagcaccg gcgcgatacg cgcgatttct
     3841 gtgtcggacg aaacaggcgc ggcgtcggga ttgctggatg ccggaccgag gtcgatcaca
     3901 tctgccccct cggccatcag cttacgcgcc tgcgcaatgg ctgcgtctgg cgccagatac
     3961 cggcctccat cggagaaact gtccgaggtt atgttgacga tgccgaaaat gatgagcgat
     4021 ttattcatgg gggcttctat aataataata atcgagcatg agtctcatac ggatgctcgg
     4081 gtcgaaaggg aatccccagg cgagtaacct gtttgcggtg atccgtttca tgatgatgta
     4141 tctgtagcat tttggaaata aatatatcat ctctcaataa gtagatatat ttatcttttt
     4201 tgcttctttg gtgctgcaag tcaccaatac gagggaaacc tgttacggag cggtttaacc
     4261 gtaatcaaca gtgactggtg tcatgttcgg aagattaatt gatagtaatc tacaaaggat
     4321 cttcttgaga tccttttcaa tgaatcgtaa tctcttgctc tgtaaacgaa aaaaccgcct
     4381 ggggaggcgg tttgttcgaa ggttaagtca gttggggaac tgcttaaccc ggtaacaggg
     4441 ttcacgagac cgccattacc aaaactgttc tttcagtgta gccgcagtca ggcctctact
     4501 tcaagacctc ttaatctatc ttctcgtgca ctcctgttac taatggctgc tgccagtggc
     4561 gttttgtcgt gtctttccgg gttggactca agttgatagt taccggacaa ggcgcagcgg
     4621 tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc cttcacggaa
     4681 ctgagtgtca ggcgtggaat gagaaaccgc ggccataaca gcggaatgac accggtaaac
     4741 cgaaaggcag gaacaggaga gcgcacgagg gagctaccag ggggaaacgc ctgggatctt
     4801 tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agattttgcg atgcttgtca
     4861 ggggggcgga gcctatggaa aaacggcttc gccgtggcct tatctcttct ttgctaagtg
     4921 tcttcctggt atcttccagg aaatttccgt ccccgtccgt aagccggtac cgctcgccgc
     4981 agccgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaatatct cctgtatcgc
     5041 atattctgct gacgcgccgg tgctgccttt tttctcctgc cacatgaagc acttcactga
     5101 ttttcgcaat cgtggcaaca tagtaagcca gtatacactc cgctagcgct acatgactgg
     5161 ttcagggctg cgccccgaaa cccgctaaca acctctcagc tactggatat caccccatgc
     5221 catctgaata tgatgaacaa ctggattcga ctgatacgca gcttcccggc gaatatgacc
     5281 tttttgattt aatggcgcag gttactgaag agaacctgca tgcccctgtg gattttgggt
     5341 cgcctgtcgg ccgggaagcg ttatagcttt ttcgcttgcc cgcgtacgtg gtaatactcc
     5401 ggtattacct tttatttctt tattttcatg taattacagt ggatttttaa tacccgggta
     5461 ttactcgtga aaacgaaata ttcctttttt ttcttttaat tcatgcagtt gcgatgaaaa
     5521 tcgcggctgc gttaggtgta tgaccatttt gaggttgaaa aataatcaac catggcgggt
     5581 gatttacgca ggttaagggg agatcgtgac aaaaaagcgt tcctcactgt atcgttcatg
     5641 cctgcatgaa cgggttattc ttcctgacta cagaattaca tcgctccttc agttcataca
     5701 tcacttttac atcacttata catcactttt acatcactta tacatcactt ttacatcact
     5761 tttacatcat tccttcagct catacgtcac tttcatcctc tgtgccattt gtacatcacc
     5821 cccttcctga acgtgctgac agcgggtttt ccggctgcgc cggcaacgct tcacggccac
     5881 gcaataaatt gcgtccccgt tcaccgtttt cggatgggaa gaacgccgga aacaggactt
     5941 accccggctg aatggtgtga atttccgcgc tatgcacttg tgcgcatact catgcatgcc
     6001 gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt ggtttgttgc ctgttttacc
     6061 ggtttcccgt cagaaacgcc ctgagggcct ctcaggcggt gctgataaca gatgttatgg
     6121 taaatcagag tcacctgcac tgttactctg aaccggagcg aaacagccgc gaccagaatc
     6181 cccgcttttt ttcctgtgcc agcgcgtcag ccagacggct aatttcggcc tgtagttgtt
     6241 ccctctcctc atttcgcagg cggttttctg cttcccgcgc catcttgtct tcaagcatta
     6301 gggtaacagt ctgctgcaga gcgtccagtt tctgctgaat gacgtctagc tgagcatttt
     6361 ccgcctggct aacgacagat ttggagtgtc cctccttgtg cctttttggt gtctcagaag
     6421 tgcgcagctc gccataggcc cgaatcagtt cactggtctc gaactcccgg cgatcatttt
     6481 ttcctgtgcg atagctgaca aggcctttag ccatgtcacg atataattgc gagcgcgact
     6541 ttcctgttaa tttcagtgtc tcttgaactg tgtgccatgc cataaaagtg acagtgtctc
     6601 ctgtgtgtac cgtattgtaa cagcagaaca tattactgtt ttaacacaaa aggtctgagg
     6661 ctgcccgtga ggcgtgaaaa atacacaaaa aacctgttga cggtgtggtt ttgacgggcg
     6721 tagtatttcg aatactgtat ataaacacat tgttataaat acagtttttg ctgtttttaa
     6781 aagtcaaaga ggataatttt
//
