LOCUS       NZ_CP049049             2507 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain Y233 plasmid p233-2, complete
            sequence.
ACCESSION   NZ_CP049049
VERSION     NZ_CP049049.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14132375
            Assembly: GCF_011044215.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Zhu,X. and Bao,Q.
  TITLE     Prevalence of Aminoglycoside Resistance Genes and Molecular
            Characterization of a Novel Gene aac(3)-IIg among Clinical Isolates
            of Enterobacter cloacae Complex from a Chinese Teaching Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Zhu,X. and Bao,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2020) The School of Life and Science, Wenzhou
            Medical University, Higher Education District, Wenzhou, Wenzhou
            325000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. v1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011044215.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:11:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,019
            CDSs (total)                      :: 4,905
            Genes (coding)                    :: 4,792
            CDSs (with protein)               :: 4,792
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 47 of 113
            Pseudo Genes (incomplete)         :: 69 of 113
            Pseudo Genes (internal stop)      :: 8 of 113
            Pseudo Genes (multiple problems)  :: 11 of 113
            Pseudo Genes (short protein)      :: 1 of 113
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049049.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Y233"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="p233-2"
                     /geo_loc_name="China:Wenzhou"
                     /collection_date="2007-01-31"
     gene            73..303
                     /locus_tag="G5B34_RS25015"
                     /old_locus_tag="G5B34_24930"
     CDS             73..303
                     /locus_tag="G5B34_RS25015"
                     /old_locus_tag="G5B34_24930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_226860856.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGGDAGAMMLD
                     AYRQILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(594..779)
                     /locus_tag="G5B34_RS25020"
                     /old_locus_tag="G5B34_24935"
     CDS             complement(594..779)
                     /locus_tag="G5B34_RS25020"
                     /old_locus_tag="G5B34_24935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1145..1372)
                     /locus_tag="G5B34_RS25025"
                     /old_locus_tag="G5B34_24940"
     CDS             complement(1145..1372)
                     /locus_tag="G5B34_RS25025"
                     /old_locus_tag="G5B34_24940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          460..547
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(1788..2316)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 tttttttttt ttcatccctg cccgctaccc tgtaaacctt tcttctgcgt tgccgttaac
       61 agtaggtaag ccatgtttgg tttttcttcc ggagccctga tgactcttag tgatgatgag
      121 cgacacctgc ttgtgtccgt tgtttcggtc tggctacggc gtgccggagg ggatgccgga
      181 ggggatgccg gagccatgat gctggatgca taccggcaga ttttgtctga aaccgaaccg
      241 gccgtaagga ctgtcatgct cgaatttctt gaatccgttc gcattcatta catttcgtcc
      301 tgagcatttt ctgtatttct gtccatactt aaacgaggcg gccgtcacgg acggtggtcg
      361 tcgttccatg atgctgttat ggacgagtga gagtaatcta atctaacttg attgagttcc
      421 gggcgtgccg cccggttttt tttgctgttt cccggtgtcc gttccgggac aggatgtgca
      481 tttgtaatac cgcgcacgcg ccggcatcac aaataccctg gtcagggctg cgccccgaca
      541 ccccgccatc gcctcacggc cgcgcactga attgcgcacc cgttcaccga ttttcacgca
      601 ttttcctcgc ctatctgtgc gttcagcatg gcgtaaagcg cttcggcctg atcgtgcagg
      661 tgctcacagc agccggcagc ctcatccaga tccatctgct cgagccgctc aaggaggaga
      721 agtgtctgtg cgcggataaa tttggccata ttgagggcgg cggattcctg tctggtcact
      781 ggtgattttc cccgtctggt gatgattttt gcgtgaaaag agcatggcgt cattctggtg
      841 catagtcatg catgccgtta aaatttaaca ggcgcgtttc tgacgggttc cggggtggtt
      901 tgttgtggtt tttgtcatgg tttcgtcagg aacgcgctga acgcgtctca ggcggtgcgg
      961 gcaacggatg ttatggtaaa ttcttggggg cttgattata aaataccgca cttcctctta
     1021 tgtcttgtgc tttttagaca accctgaaag cttttagctt tggtgatgca atttgctggg
     1081 attttggcgg agaaccacag gtaaagaaaa aggccacatt agcggccttt ttcggagaag
     1141 atgctcagtg cggaatttcg gagacttctt taacgtcggt tttattgatc tgctgtttga
     1201 caccattcgc gtctgtatag ccaagcaggc ctgagtctga ttctttaggt ttgccgtcgc
     1261 tgacaatagt gcgaccgtca ttggtgtgta tggcatagct tgtgcgcgtg cagccggtga
     1321 gggctgaaag cgcaactgcc gctgcgaaca ctgaaacaaa aatctttttc aaagccagct
     1381 ccttttatcc atatcagttt tggtcactaa ccatctaact ataggtcatt ttttatgtga
     1441 aaagaggctt gatggtgggc tgtatatcga gttaggcgcg aggtgctatg gtcaaagtgt
     1501 ggtgtcaggc ggggaggagg cttatatgag atgtaatccg gccgatacat ataccaagat
     1561 gatttaagta ttcctctttg tgaggttgga tgtctaattt ataaaggatc ttcttgagat
     1621 cctttttttc cgcgcgtaat ctcttgccct gtaaacgaaa aaaccaccct ggcaggtggt
     1681 ttttcgaagg ttaggtaatc ctggcagatc ccctaaccgt ggtaacagtc ttgtgcgaga
     1741 catgtcacca aatttgtcct ttcagtgtag cctcactaag gccgccactt caagaactct
     1801 tgagacatct ctcgcacatc ctgtttgcca atggccgttg ccaatggcga ttagtcgtgt
     1861 ctttccgggt tggactcaag ttgatagtta ccggataagg cgcagcggtc ggactgaacg
     1921 gggggttcgt gcatacagtc catcctggag cgaactgcct tcccggaact gagtgtcagg
     1981 cgtggaatga gaaaccgcgg ccataacagc ggagtgacac cggtaaaccg aaaggcagga
     2041 atgcggggga gcacgaggga gccaccaggg ggaaacgcct ggtatcttta agccgcatcg
     2101 ggtttcgcca ccactgattt gagcgtcaga ttctgtgatg cttgtcaggg gggcggagcc
     2161 tatggaaaaa cggctttggc gcggccttat gctttcttcg ttaagtatct tcctggcatc
     2221 ccccaggaaa tttctgatcc atccgtaagc ccgtcccgct cgccgcagcc gaacgaccga
     2281 gcggagcgag tcagtgagcg aggaagcgga atatattctg tatcacattt tctcctgacg
     2341 cgttttcttt cactttctgc gcctgtctta tgtggcatta atgctatgtg ttactgccat
     2401 gctacatctt aagccagtat acactccgct agtgctccgt gactggtccg gcgctgcgcc
     2461 cggaacccgc ctgtaccggt tcagcagccg ttccggcctg actgcaa
//
