LOCUS       NZ_CP049027             2746 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-E3 plasmid pEclE3-6, complete
            sequence.
ACCESSION   NZ_CP049027
VERSION     NZ_CP049027.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747520
            Assembly: GCF_015910345.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-FEB-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910345.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:53:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,564
            CDSs (total)                      :: 5,448
            Genes (coding)                    :: 5,307
            CDSs (with protein)               :: 5,307
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 44 of 141
            Pseudo Genes (incomplete)         :: 98 of 141
            Pseudo Genes (internal stop)      :: 18 of 141
            Pseudo Genes (multiple problems)  :: 18 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049027.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-E3"
                     /isolation_source="surface water"
                     /db_xref="taxon:158836"
                     /plasmid="pEclE3-6"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2015-08-04"
                     /collected_by="Public Health Ontario"
     gene            590..817
                     /locus_tag="GVI75_RS27670"
                     /old_locus_tag="GVI75_27670"
     CDS             590..817
                     /locus_tag="GVI75_RS27670"
                     /old_locus_tag="GVI75_27670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_225622915.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRACMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            complement(865..1053)
                     /locus_tag="GVI75_RS27675"
                     /old_locus_tag="GVI75_27675"
     CDS             complement(865..1053)
                     /locus_tag="GVI75_RS27675"
                     /old_locus_tag="GVI75_27675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1382..1756
                     /locus_tag="GVI75_RS27680"
                     /old_locus_tag="GVI75_27680"
     CDS             1382..1756
                     /locus_tag="GVI75_RS27680"
                     /old_locus_tag="GVI75_27680"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061335866.1"
                     /translation="MNGMSDVRLKKSTIESLKEYVNDFGMTVDDVVLNLIEENNSLKSL
                     LGQGFSLPGGDKDTHSFKDYVIHKLPKGKILVTKDGLKVSNMKKVLIEAGSAVGMDDKE
                     LNPDFTTYEIGRFLFKHLQG"
                     /name="hypothetical protein"
     gene            complement(1945..>2046)
                     /locus_tag="GVI75_RS27685"
                     /old_locus_tag="GVI75_27685"
                     /pseudo=""
     CDS             complement(1945..>2046)
                     /locus_tag="GVI75_RS27685"
                     /old_locus_tag="GVI75_27685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011847251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /name="IS30 family transposase"
     Typing          complement(2368..2744)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.767"
                     /coverage="82.0"
                     /evalue="2.49e-171"
ORIGIN
        1 ctgttaagct tcttcctgga agatgccagg aaatttccgc cccgccagta agccggtacc
       61 gctcgccgca gccgaacgac cgagcggagc gagtcagtga gcgaggaagc ggaatatctc
      121 cgggcttatc tttggcaccg tacgcgccat agctgcattt tttaatacga tgcaggatag
      181 ggcaaacgcc gcaaaatgac gtcatccgac atcattccgc cgcgttcgtc cagtgaccta
      241 cagtcggctc tggggtgtcg gggctgtgcc ctgaccaggc gttttggagt atcgccaccg
      301 tggtggcggt gctacaaaac atatcctgtc ccttccttgg catctctgaa gtttagccag
      361 tatacactcc gctagcgcta cgtgactggt tcagggctgc gccccgacac ccgctaaaag
      421 cactgacgcg cctgcggctt gcccaacccc gcgccgtccg ggaaagaatt tcccgatcga
      481 cccgacgttt ctgtttacct gtgatgttaa cgtttggatc tttttcccgg gtttacctat
      541 tttcttctaa tgaagcgttc acagctgacg ttaatagatg ggtgctttca tgtttggttt
      601 ttcaccagga gaacttatga acctttgccc cgatgaacgt ctgctctttg tgcggatgat
      661 ttccgctatg cttcgccgtt cgggcggtga tgcgggtgct gttatgtttg aggcgtatcg
      721 ccatattgtg tccgatacca atcaagctcg tcgtgcctgc atgctcgacc tgctcgaaag
      781 cgtcaggcat gactatgtac acggtggata tacatagttg ccctggatac gcttttcgtc
      841 taagtgagcg ttcttttcgt ctctttattc gccgtcctgt ataccgaagc gtgcgctcag
      901 cttctgataa agcgcttctg cctgttcgtg cattcgctca cagtcggctg cctcgtcatc
      961 aagatcgagc acgtcgagtt tttcgagcag cagcagcgac tgcgcccgga taaattttgc
     1021 catgttcagt gcggctgatt cctgttttgt catacggctt tttcccggtt ttatggtgat
     1081 cgcagactga aatcttactt cattcacttg agtgcatagc catgcaggta cgcagaaatc
     1141 atcagcgcgt ttctgagcgg ttttcagggg gtaagatgtg ttcttttgca ggtgaggtcg
     1201 tagaaacgcc ctgagagcct ctcaggcggt gcacgcaacg gatgttatgt tcaatctggc
     1261 ggtgcggcat agttatcatc gcacctacag accgcttgtt gtattcagaa caatatttat
     1321 ttgtataatt tgtattttgt tgtattattt cttcaaatta gagcggtggg gtaataataa
     1381 tatgaatggt atgtctgacg taagactaaa gaaatcaact attgagagcc taaaagagta
     1441 cgtgaatgac tttggcatga cggttgatga tgttgtcctg aacttaattg aagagaataa
     1501 ttcattaaaa tcgttattag gtcagggctt ttctttgcct ggtggagata aggacacaca
     1561 ttccttcaag gattacgtga ttcataagct tccaaaagga aagatcttgg ttaccaaaga
     1621 cgggcttaaa gttagcaaca tgaagaaggt attgatcgag gcagggagcg ctgtagggat
     1681 ggatgataaa gagctaaacc ctgactttac gacctatgaa ataggccgtt ttctcttcaa
     1741 acatcttcag ggataaaggt gtctaccagt tcgtggccgg gattttaggt tcaattcagc
     1801 tagttcccgg acagaaaaaa agccaccgtg taggtggctt ttttattcct gctttaggca
     1861 aagcattatt gatagcattt gcaagcaaac cgttagcgac ttctgctgaa tgcttctggg
     1921 cggattcaag ttcgaagtgc aacgctaagc cgcctgtctt agctcagcaa agaccactga
     1981 aggttgccgg aatccaagac atttccgtgg cctgctattc agtgctctct ggtacttgcg
     2041 gagctcccct gtgtgataag tcaggtgtgg aatttttacg tataaggccc cgctaggcgg
     2101 aaaccaatac tgaagccaaa tcactggtaa acagccggat cttcttgtga tccttttact
     2161 ctcgtcgtaa tctcatgtcc tgtaaacaaa aaaaccaccg ctaccaacgg tggttcgttt
     2221 gccggaatca agagctacca actctttctt ccgaaggtaa ctggctttca cggagcgcag
     2281 ataccaaaac tgtccttcca gtgtagccgc agtcaggccg ccacttcaag aactctgtag
     2341 aagcactcta ctaccctgct ccgtgaatcc tcttaccaat ggctgctgcc aatggcgtta
     2401 agtcgtgtct ttccgggttg gactcaagac gatagttacc ggacaaggcg cagcggtcgg
     2461 actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga
     2521 gtgtcaggcg tggaatgaga taaacgcggc cataacagcg gaatgacacc ggtaaaccga
     2581 aaggcaggaa caggagagcg cacgagggag ccaccagggg gaaacgcctg gtatctttaa
     2641 gtcctgtcgg gtttcgccac cactgatttg agcgtcagat ttcgtgatgc ttgtcagggg
     2701 ggcggagcct atggaaaaac ggcttctccg tggccttctt gcttcc
//
