LOCUS       NZ_CP049026             5112 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-E3 plasmid pEclE3-5, complete
            sequence.
ACCESSION   NZ_CP049026
VERSION     NZ_CP049026.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747520
            Assembly: GCF_015910345.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-FEB-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910345.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:53:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,564
            CDSs (total)                      :: 5,448
            Genes (coding)                    :: 5,307
            CDSs (with protein)               :: 5,307
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 44 of 141
            Pseudo Genes (incomplete)         :: 98 of 141
            Pseudo Genes (internal stop)      :: 18 of 141
            Pseudo Genes (multiple problems)  :: 18 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049026.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-E3"
                     /isolation_source="surface water"
                     /db_xref="taxon:158836"
                     /plasmid="pEclE3-5"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2015-08-04"
                     /collected_by="Public Health Ontario"
     gene            complement(236..439)
                     /locus_tag="GVI75_RS27640"
                     /old_locus_tag="GVI75_27630"
     CDS             complement(236..439)
                     /locus_tag="GVI75_RS27640"
                     /old_locus_tag="GVI75_27630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_197377787.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELALDVEADLCEKLHDDA
                     EHLFRTLSSRLDELGVDRENGK"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            855..968
                     /locus_tag="GVI75_RS27645"
                     /old_locus_tag="GVI75_27640"
     CDS             855..968
                     /locus_tag="GVI75_RS27645"
                     /old_locus_tag="GVI75_27640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            complement(1205..1573)
                     /locus_tag="GVI75_RS27650"
                     /old_locus_tag="GVI75_27645"
     CDS             complement(1205..1573)
                     /locus_tag="GVI75_RS27650"
                     /old_locus_tag="GVI75_27645"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045626380.1"
                     /translation="MKTTDRFISVSFRYFHNVDDIGQSIDSWRVDGSLPDLIEKLVYLT
                     ANGITKVSSDGIFTNYTRFPDSAVNDFKCPDEISVDENWGVIKNIGGQKRRVAGFLKDN
                     VFYIVFFDKDHRFWKSRK"
                     /name="hypothetical protein"
     gene            complement(1646..2944)
                     /locus_tag="GVI75_RS27655"
                     /old_locus_tag="GVI75_27650"
     CDS             complement(1646..2944)
                     /locus_tag="GVI75_RS27655"
                     /old_locus_tag="GVI75_27650"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_097414638.1"
                     /translation="MMVNFSLSNAFSFMDAQNMSMLAVSSAKDDINLANVVSVSDVRIL
                     KSALIFGANASGKSNLMKTISLMKDIVLHSVQAIESSITKNVIPFLLDEDGPNKPSEME
                     MTFISDGVKYRYGLSIFQGKIKEEWLYYTPKSRETILFERDGLSIEVNKAAFSESNLFI
                     KEGIIQKTREDVPFVSVVAGFNGEHSKRVVNWFNNIVFLSGSNEGMFTAITMKLINESE
                     DFKAWLKKILPAFQINDIYLDEVAPDFPFGNIKTDDDNLKNLFSSIQNFAKNQKVFSLR
                     VVKKTASGEISVPIDFESEGTKKIVYLLGPIYNAMINGNLLLVDEFDSKFHTLLSKFLF
                     RIFHAQSEGLSQIIAAVQDVNLMNTDCFRRDQIWFVDKDIKSGSSHLYSLVEYKEKQRT
                     LKSSYGHDYLSGAFDAIPLFDNLDEFEKLMPEG"
                     /name="ATP-binding protein"
     gene            3639..3905
                     /locus_tag="GVI75_RS27660"
                     /old_locus_tag="GVI75_27655"
     CDS             3639..3905
                     /locus_tag="GVI75_RS27660"
                     /old_locus_tag="GVI75_27655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072050235.1"
                     /translation="MSVYCVSYDLNQAGQNYNALYDELKKSPGWCHPLDSTWLISTGET
                     AQQLSDRLRRHLDNNDTLLVIGVTKEYAGWLTQDTWDWMRTDI"
                     /name="hypothetical protein"
     gene            complement(4689..5111)
                     /locus_tag="GVI75_RS27665"
                     /old_locus_tag="GVI75_27660"
     CDS             complement(4689..5111)
                     /locus_tag="GVI75_RS27665"
                     /old_locus_tag="GVI75_27660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_045265535.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          561..968
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.484"
                     /coverage="99.0"
                     /evalue="1.3e-157"
ORIGIN
        1 agttaccatc ctgtaattgg ggtcgtctca gaattcggaa aataaagcac gctagcggtt
       61 gatctgtcag gttgaagcct gagaggccga gcgcagatcg tcagaaaagg cgaaaaacga
      121 tcctaatctg acgcaacata ggtggggtgc ctgacgcccg gttgaggcgt acttcaactg
      181 gacaccattc cagaaagacc aagcatggca tggcctgccg ctgtcttacc gtgctttatt
      241 tcccgttttc tctatcgacc cctaattcat ccagtctcga ggacagtgta cggaaaagat
      301 gctccgcatc atcgtggagt ttctcacaaa ggtcggcctc gacatcgaga gcaagctcgt
      361 taagcttctc cagcaggagc agagactgat tctggatgaa tttagccatg ttcagcgctg
      421 ttttctgttg tttcgtcata agaagcctca gagcgtcagt gctcagtgta aaaccagtct
      481 cttttccgct tcctcgctca ctcagtcgct acgctccggt cgttcggctg cggcgagcgg
      541 caagaccatt cttataaaag ctcgagaaaa ccgagcgatc aaggtaggag gaaaagagga
      601 ggccgcgcag cggccgtttt tccataggct ccgcccccct gacaagcatc acagaaacgg
      661 acgctcaaat cagtggtggc gaaacccgac aggactataa agatacctat catttctgat
      721 aggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg ccattccgct
      781 gttatggccg cgtttatctc attccacgcc agacactcag ttccgggtag gcagttcgct
      841 ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ctgctgcgcc ttatccggta
      901 actatcgtct tgagtccaac ccggaaagac atgcaaaagc gccactggca gcagccactg
      961 gtaactgatt tagaggagtt agtcttgaag ttatgcgccg gttaaggcta aactgaaaga
     1021 acaagttttg gttactgcgc tcctccaagc cagttacctt ggttctaaga gcgccaactc
     1081 ttggaacctt cgaaaaaacc gcccgccaag gcggtttttt cgtttacaga gcgagagatt
     1141 acgacgagaa cacagtgatc tcaagaagat cttgcctcaa catacagtac aatcgtaact
     1201 aagcctattt cctgctcttc caaaaccgat gatccttatc aaaaaaaact atataaaaca
     1261 cattatcctt taaaaaacca gcaacccttc ttttctggcc tcctatattt tttataacac
     1321 cccagttctc atcaactgat atttcatcag ggcatttaaa atcattaact gctgaatctg
     1381 gaaatcgagt gtagttagta aaaataccat cagagctgac ttttgttatc ccattagctg
     1441 taagataaac aagcttttca attaaatctg gcaaactgcc gtcaaccctc caagaatcaa
     1501 tagactgacc aatatcatcg acgttatgaa aatatctaaa actaacagaa ataaatctat
     1561 ctgtagtttt cataatgctc tttgaggatt ttgtttcctc tttcctcgaa gagctacgaa
     1621 gaccgcggcc tctctttgac ataaatcatc cctcaggcat aagtttttca aattcatcta
     1681 aattatcaaa caatgggata gcatcaaatg caccagataa ataatcatgc ccatacgatg
     1741 attttaaagt tcgctgcttc tctttatact caaccaaaga atagagatga ctacttcctg
     1801 attttatatc tttatcaaca aaccaaatct ggtcacgtct aaaacaatca gtgttcatta
     1861 gattaacatc ctgaacagca gctattattt ggctaagccc ctcgctttgc gcatggaata
     1921 ttctaaacaa aaacttagac aaaagggtgt gaaacttaga atcaaactca tcaactaaca
     1981 ataagttacc gtttatcatg gcattgtaga ttggaccaag aagataaact atctttttag
     2041 taccctcaga ctcaaaatca ataggtactg aaatctcacc gctagctgtt tttttcacta
     2101 ctctaagaga aaaaaccttc tggttttttg caaaattttg tattgaacta aataaatttt
     2161 ttaaattatc atcatctgtt tttatatttc caaatggaaa atcaggtgcg acctcatcga
     2221 ggtaaatatc attaatctgg aatgctggaa gtattttttt tagccaagcc ttaaaatcct
     2281 cagactcatt tattaacttc atggtaattg ctgtaaacat cccttcatta gaaccagaaa
     2341 ggaatacgat attgttaaac caattaacta ctcgttttga atgctcacca ttaaaaccag
     2401 caacaaccga aacaaatgga acatcctccc ttgttttttg aataatccct tctttgataa
     2461 aaaggtttga ctcactaaaa gccgccttat ttacctcaat agaaagacca tctctttcaa
     2521 agagaatggt ttctcttgat tttggtgtgt agtacaacca ttcttctttt attttcccct
     2581 gaaaaataga caatccatat ctatatttaa ccccatctga aataaaagtc atttccattt
     2641 cagatggttt atttggtcca tcctcatcaa gcaaaaacgg tattacgttc tttgtaatgc
     2701 tactttcaat agcttgcacg gaatgcaaaa caatatcttt cattaaagaa attgttttca
     2761 tgagattaga cttcccgcta gcgttagccc caaatatcag agcagatttc agtatccgaa
     2821 catcactcac tgaaaccacg ttagccaagt ttatgtcgtc tttcgcggat gacaccgcca
     2881 acatgctcat attttgagca tccataaagg aaaatgcgtt agaaagacta aaattaacca
     2941 tcataagaac accccttacg ccatccttaa acaacaaaat gagtaaatat tgacaaaatc
     3001 aggacttaag gatagcatac aagcgttcta aaacaacagg tgcgtttaat agatagcacg
     3061 ttatggaaca cttgttaaaa aaagctaaat gcagggggtc tatgagattt tttctatacc
     3121 aaatggttcc tttagccgca gttgtcagct cagcagtgct agctttacac tcggtttcag
     3181 gatggggatg gtttctcttt gtagcattta tgctcagcgg agctgataca gtgaagaaac
     3241 atctaggaaa tgacgcatga aaggagaaaa acgacgtttt ttgaagtaag tgggttagcc
     3301 tgtaccacca gaactaaccc gtaacaaacc atacacaaat gcggcacttg agtaccacct
     3361 caagagcttg ataaagccag ccacaagatt tgttggttgc tacagtacaa ttttgatagc
     3421 aaaaatcaac actcatgtgg cgaaaaatag gaaatgcacc atgcagacag acgagtctca
     3481 ggcatacatt tttcgccctt atatcacagt aaagggcaaa cggatcaccc gtccaaacgg
     3541 tgggatgttc aaaattccaa ttaatcgtga aaagaagaag taagttgtga ctgcagccct
     3601 aacgggctgc cccacatttg attaaagagg atggttctat gtcagtttac tgtgtctctt
     3661 atgacctgaa tcaagcaggt caaaactaca atgcactata tgatgagtta aaaaaatcgc
     3721 cggggtggtg ccacccttta gattccacat ggctaatatc aaccggagaa acagcgcaac
     3781 aactgtcgga tcgtttgcgt aggcatctgg acaataatga caccttgctt gtgattggtg
     3841 taaccaaaga atacgcagga tggctcaccc aagacacatg ggattggatg agaacagata
     3901 tatagatata aagccctgcg gggctttttt tacgtctgta gctggtgtgg atttaacata
     3961 accccctaat tgtacgcacc gctaaaacgg cctcagggcg tttctgacgg gaaaccggta
     4021 aaacaggcaa caaaccaccc gaaaacccgc cagaaacgcg caggctctgt ttttacggca
     4081 tgcatgagta tgcacgcaag tgcatagcgc gaaaattcac gccattcagc cagggtaagt
     4141 cctgtttccg gcgttcttcc catccgaaaa cggtgaacgg ggacgcaatt tattgcgtgg
     4201 ccgtgaagcg ttgccggcgc agccggaaaa ccggttttca gcacgttcag aaatcgggtt
     4261 cgtcgacggc cggcaatcac gttatggcaa gtcgccggac gactcaccac acgattcacc
     4321 ggcgaatctt cactagagtc gtacccagag tcgccgagtc taaaaaacaa ccaaacgggg
     4381 agtcgtgtct agagtcgggt cgttattaac ttattgtttt tatagtcctg agtcgttacc
     4441 gactctatga cgactcgccg gggactctat gacgactcgt catagagtcg gatctatata
     4501 aataaaattt aaaaaacgaa atttcaggaa agtaacccac tgatattaaa tacaaatctg
     4561 caaaatgtta attttgcgtg gggtgtgggt atttttagtg gtgaggagcg ttcagagatc
     4621 tgacgatttt tgggaaggtt caggaactcc ccgggatggc cggagaggaa aacggaagca
     4681 tgacggggtc aggatttttt ccagaatttc caccacgggg aatggggtgg tgaagaaggt
     4741 tcgtgcttgt gctccagaag ctgcatagcc tgacgaaggg aatcaatatg ctcatctcga
     4801 gccttaacag cccctttaag accatcgttt tcagcttcaa gtaaagcgat tctatgttgt
     4861 aaatcatgta aaccaacgct gttaacacct gtagattctg cgtcactttt tacatcatat
     4921 tttacagtat cgatgacacc atcaagagaa ccaaacactc taataagctc tgaagtatca
     4981 ataactttaa tagccttacc gtgctttaca acagaggtgt aacttaactt cccactgtta
     5041 atcatcttgt aaattgtggg gcgactaacc ttaacaagcc gtgccgcttc actaatagac
     5101 actctggcca tc
//
