LOCUS       NZ_CP048919             2088 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Escherichia coli strain E2 plasmid pE2-4, complete sequence.
ACCESSION   NZ_CP048919
VERSION     NZ_CP048919.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13031456
            Assembly: GCF_018884065.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Gamal,D., Fernandez-Martinez,M., El-Defrawy,I., Ocampo-Sosa,A.A. and
            Martinez-Martinez,L.
  TITLE     First identification of NDM-5 associated with OXA-181 in Escherichia
            coli from Egypt
  JOURNAL   Emerg Microbes Infect 5 (4), e30 (2016)
   PUBMED   27048740
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Moreno-Mingorance,A., Fabrega,A., Gamal,D., Martinez-Martinez,L. and
            Gonzalez-Lopez,J.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2020) Microbiology, Hospital Universitari Vall
            d'Hebron, Pg Vall d'Hebron 119-129, Barcelona 08035, Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-AUG-2019
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 70.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018884065.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 03:33:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,893
            CDSs (total)                      :: 4,776
            Genes (coding)                    :: 4,521
            CDSs (with protein)               :: 4,521
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 255
            CDSs (without protein)            :: 255
            Pseudo Genes (ambiguous residues) :: 0 of 255
            Pseudo Genes (frameshifted)       :: 85 of 255
            Pseudo Genes (incomplete)         :: 166 of 255
            Pseudo Genes (internal stop)      :: 52 of 255
            Pseudo Genes (multiple problems)  :: 39 of 255
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048919.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E2"
                     /isolation_source="ascitic fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pE2-4"
                     /geo_loc_name="Egypt:Giza"
                     /lat_lon="30.094190 N 31.224056 E"
                     /collection_date="2015"
                     /collected_by="Theodor Bilharz Research Institut"
     gene            join(1814..2088,1..691)
                     /locus_tag="G5S48_RS24255"
                     /old_locus_tag="G5S48_24165"
     CDS             join(1814..2088,1..691)
                     /locus_tag="G5S48_RS24255"
                     /old_locus_tag="G5S48_24165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_039064478.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLAIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            695..961
                     /locus_tag="G5S48_RS24260"
                     /old_locus_tag="G5S48_24170"
     CDS             695..961
                     /locus_tag="G5S48_RS24260"
                     /old_locus_tag="G5S48_24170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          120..351
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 ccgtttttat caggctctac cgaaaatcgt tgtggattac ccgtcttccc gatggttgtt
       61 tctgacgtta actgtcagga actgcgagat aggtgaactt ggaacagtcc ttacagcaat
      121 gaatgcggcg tttaagcgaa tggaaaagcg aaaggagcta tcacctgttc aggggtggat
      181 cagggctacg gaggtgacgc gaggtaagga tggcagcgca catccgcatt ttcactgtct
      241 gctgatggtg caaccttctt ggtttaaagg gaagaactac gttaagcacg aacgttgggt
      301 agaactctgg cgcgattgct tgcgggtgaa ctatgagccg aatatcgata ttcgggcagt
      361 aaaaactaag acaggtgagg ttgtggccaa cgttgccgag caactgcaaa gcgcggttgc
      421 tgaaacgctg aaatactccg ttaaaccgga agatatggca aacgatcctg agtggtttct
      481 tgagctgacg cggcagcttc acaagcgccg ttttatctcg accggtggag cgctaaaaaa
      541 cgtcctccag ttggatcgag aaaccaatga ggatcttgcc attgccgacg atgtagggga
      601 tggcactgat gacgggaagc ggacggcgtt tgtctgggat tcaggtaaac ggcgttacaa
      661 acgcgcccct gagaaggata aatcggatta acgtatgaat attaatattg aatacctgaa
      721 tggaaataag actattggtt tatttttttt aagaagtgaa gcggtgattc ctgacaggtt
      781 taaaaacctt attttgctta ttgatggatt aagttttggc acatttggtt ttcatccgca
      841 cgaaggtttt gaggatgaat taattttata tattcagaaa acaaacgaga gggtaaaaac
      901 tctttttgtg aaaatagatt taaacggtat caagtttgga tttttaagaa cgcattctta
      961 gttctggaaa agagccagcg gcaggctgag gtgataggta cgagattgca tgcaatctct
     1021 agtgctctgt ctatcctgca ttatcctcag cattatcctc agccttgcca actcgacacc
     1081 aatgcaggat agacaatccg atgtcaaatg ttaacactct gcgagtggta cattttcccc
     1141 ggattatcgt cctgagcctg ccgctggctc tctttctacc gcctcgcttt gctcgttgct
     1201 caacgcctca cagacacgga ttaaaatccg catccgttca ccgtttttta aagtccgtta
     1261 aaagcatgat gccatctccg agagttaatc tcgtcaaatg ctaaatcgtg ggggtcccct
     1321 ttggggttcc gatttagtga ttgacgacac caccgattaa aaaacttatg cggggtggat
     1381 ggtttcacga agtgaggcca tccacctgta agacagggtt ttgtttttat cccctgtttt
     1441 ggtgatcggg tgtgtggaaa aggttggggt aagccgttcg gggtgcttgt tttggggggt
     1501 taaaattgtg gttatttttt gcgcaattct cgcgcgtgat ccttgtattt atacttaagg
     1561 gataaatggc gggtatgaaa tagtggttta gcccagtaat gacgaggctt tgagtgggtt
     1621 ttgacaggtc aaagaaaatg gagcagaatt gaggcgtttt taatcggcgt tggggagtgc
     1681 gtcaacactc cccaacattt cgaatgtgtc acctcagcgg caaactctgg tgacatgtac
     1741 tggctcgcaa tgcacaggta cgtgatgaat ataccacatc aaatcacagc ctgcccagat
     1801 cggagcaggc ttaatgtcag aagataaatt cctttcggac tacagccccc gtgatgcagt
     1861 ttgggatacc cagcgcacgc ttaccgattc tgtcgggggt atctaccaga ctgctgctga
     1921 attcgagcgc tatgcactcc gtatggcctc ctgtagcggt ttgttacgtt ttggttggtc
     1981 taccatcatg gaaaccggag aaacgcgcct acggcttcgt agtgcgcaat tttgccgtgt
     2041 ccgtcattgc cctgtctgcc agtggagaag aaccctcatg tggcaagc
//
