LOCUS       NZ_CP048826            33309 bp    DNA     circular CON 07-JUN-2024
DEFINITION  Escherichia coli strain QEC11-421 plasmid pEcQE11-421-5, complete
            sequence.
ACCESSION   NZ_CP048826
VERSION     NZ_CP048826.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14049568
            Assembly: GCF_016446335.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33309)
  AUTHORS   Wang,Y., Liu,H., Wang,Q., Du,X., Yu,Y. and Jiang,Y.
  TITLE     Coexistence of blaKPC-2-IncN and mcr-1-IncX4 plasmids in a ST48
            Escherichia coli strain in China
  JOURNAL   J Glob Antimicrob Resist 23, 149-153 (2020)
   PUBMED   32966910
REFERENCE   2  (bases 1 to 33309)
  AUTHORS   Wang,Y., Liu,H., Wang,Q., Du,X., Yu,Y. and Jiang,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2020) Department of Infectious Diseases, Sir Run
            Run Shaw Hospital, Zhejiang University School of Medicine, Qingchun
            East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Assembly Name                     :: QE11-421
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016446335.1-RS_2024_06_07
            Annotation Date                   :: 06/07/2024 02:13:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,067
            CDSs (total)                      :: 4,944
            Genes (coding)                    :: 4,698
            CDSs (with protein)               :: 4,698
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 70 of 246
            Pseudo Genes (incomplete)         :: 172 of 246
            Pseudo Genes (internal stop)      :: 47 of 246
            Pseudo Genes (multiple problems)  :: 36 of 246
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048826.1.
            Bacteria and source DNA available from Quzhou People Hospital.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..33309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="QEC11-421"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEcQE11-421-5"
                     /geo_loc_name="China: Quzhou"
                     /lat_lon="28.97 N 118.88 E"
                     /collection_date="2011"
                     /collected_by="Jun Lu"
     gene            complement(282..809)
                     /locus_tag="QE11421_RS24845"
                     /old_locus_tag="QE11421_24620"
     CDS             complement(282..809)
                     /locus_tag="QE11421_RS24845"
                     /old_locus_tag="QE11421_24620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="WP_000577043.1"
                     /translation="MIFNRNKKRDEQHNSAVVLPSAENVFSDFIKNNPALTTNAFTAIG
                     EVDCFTKVPLLSNNESEFLGILSRNIIPDYSVFPKIRLIEFVRPHGSEEAVKDLIREVQ
                     NITCDFVLKSYDETVLAVIQFGETATVRQQKKKLIIQRVCAMTGISFFEFKNTVDMMGS
                     EDFCQFLRDDEE"
                     /name="DUF2726 domain-containing protein"
     gene            complement(944..1348)
                     /locus_tag="QE11421_RS24850"
                     /old_locus_tag="QE11421_24625"
     CDS             complement(944..1348)
                     /locus_tag="QE11421_RS24850"
                     /old_locus_tag="QE11421_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012421203.1"
                     /translation="MKDNIVDFQRIRDSMNDHSTDVANDAVYSDKKKISRFFYLLTPVR
                     YLLAASLYLVVVIPLGILAGFRFLLQFLGGIAVMGLTIAWYMGADVSSYVVLAAWIALT
                     VCGCAEQIFEWWIESRFAFRLFGIGRTPEE"
                     /name="hypothetical protein"
     gene            complement(1361..2227)
                     /locus_tag="QE11421_RS24855"
                     /old_locus_tag="QE11421_24630"
     CDS             complement(1361..2227)
                     /locus_tag="QE11421_RS24855"
                     /old_locus_tag="QE11421_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473366.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001353743.1"
                     /translation="MTTNKTSLSRLTKVRHRNELNSTLSTLPMAAKKVLFLAMCQINSK
                     NEFDDDHIFYVTVADYIKWVQVKPDAAYLALRDGSNILDTTLLKLKHDEILELSSDLGF
                     KFTKSNVPDSMNLSLTVFSTYYRNEGRVGIKFTKEAKRFLCKLIGEEKRYTTQVLLSVV
                     RLTSVNAASLYQLIRKIYSNNSRANSFEMTIDELKDELNLYTIGAGGVKDYKYPDYPAF
                     KRDVLNKSVKEIMKHTEVKNLSFVVSEKIGRKVYKLKFSYTIGYEGDTREDSEFTNMFD
                     KMYPPEN"
                     /name="replication initiation protein"
     gene            3642..4157
                     /locus_tag="QE11421_RS24860"
                     /old_locus_tag="QE11421_24635"
     CDS             3642..4157
                     /locus_tag="QE11421_RS24860"
                     /old_locus_tag="QE11421_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025395.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELMK
                     AVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVWIS
                     GETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRRATG
                     WQRVETRA"
                     /name="J domain-containing protein"
     gene            4262..4804
                     /locus_tag="QE11421_RS24865"
                     /old_locus_tag="QE11421_24640"
     CDS             4262..4804
                     /locus_tag="QE11421_RS24865"
                     /old_locus_tag="QE11421_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001083906.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKNY
                     SVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVLQA
                     IEYEKHQVEKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDSYDLQIR
                     GLSIDQLVKITGYLKQL"
                     /name="hypothetical protein"
     gene            4801..5052
                     /locus_tag="QE11421_RS24870"
                     /old_locus_tag="QE11421_24645"
     CDS             4801..5052
                     /locus_tag="QE11421_RS24870"
                     /old_locus_tag="QE11421_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001230707.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDA
                     VLNENELQHIDFTCAGLREDDETPVKNLIKEFMLNETR"
                     /name="hypothetical protein"
     gene            5039..5266
                     /locus_tag="QE11421_RS24875"
                     /old_locus_tag="QE11421_24650"
     CDS             5039..5266
                     /locus_tag="QE11421_RS24875"
                     /old_locus_tag="QE11421_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000801440.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQN
                     SQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
                     /name="hypothetical protein"
     gene            5720..6019
                     /locus_tag="QE11421_RS24880"
                     /old_locus_tag="QE11421_24655"
     CDS             5720..6019
                     /locus_tag="QE11421_RS24880"
                     /old_locus_tag="QE11421_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000650309.1"
                     /translation="MKCSSVFTSTTNHVFTFERVTLCTIILMHKDTGQQYVVIFTDNNK
                     IRDYKAGIVPQFGELKQSDVDLVLFYRDEYEKYFDSLKDGDECLSFKDFIECLC"
                     /name="hypothetical protein"
     gene            6110..6460
                     /locus_tag="QE11421_RS24885"
                     /old_locus_tag="QE11421_24660"
     CDS             6110..6460
                     /locus_tag="QE11421_RS24885"
                     /old_locus_tag="QE11421_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000854259.1"
                     /translation="MKTLKQAAMQFASDLRNNRCFKAAYNDALLELDREDLNAIVQCTF
                     LFEPKRTLAELDKLIKKIEVEEDYEFCVSLDKGKGLHEVRTFKSEREALDAYRILTLYG
                     YTVTIEKKEGND"
                     /name="hypothetical protein"
     gene            6765..7013
                     /locus_tag="QE11421_RS24890"
                     /old_locus_tag="QE11421_24670"
     CDS             6765..7013
                     /locus_tag="QE11421_RS24890"
                     /old_locus_tag="QE11421_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653200.1"
                     /GO_function="GO:0003729 - mRNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family toxin"
                     /protein_id="WP_000520549.1"
                     /translation="MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFF
                     NAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAGLL"
                     /name="type II toxin-antitoxin system HicA family toxin"
     gene            7010..7357
                     /locus_tag="QE11421_RS24895"
                     /old_locus_tag="QE11421_24675"
     CDS             7010..7357
                     /locus_tag="QE11421_RS24895"
                     /old_locus_tag="QE11421_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="WP_000681613.1"
                     /translation="MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQ
                     EFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPELHRRVAEMAMEEDLSLNAFVNKAL
                     EKEVSNHRAGA"
                     /name="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            7718..8221
                     /gene="taxA"
                     /locus_tag="QE11421_RS24900"
                     /old_locus_tag="QE11421_24680"
     CDS             7718..8221
                     /gene="taxA"
                     /locus_tag="QE11421_RS24900"
                     /old_locus_tag="QE11421_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxA"
                     /protein_id="WP_000781813.1"
                     /translation="MKMIAFRLSDDELKFAEHNAILSGFTSINAFAKHNVLNIETKPVN
                     IPVNNEPAKLVSVRLYPHEIELVKRNAALHGMSMSREIAIRVRQSLLKSEVCLYPDEVK
                     ELKKLSTAVDRVGRNIHFIIKGERFCTVNDPDFRKDVIEVIELCKQIDSKLETLTKSVV
                     NRFG"
                     /name="taxA"
     gene            8225..9454
                     /gene="taxC"
                     /locus_tag="QE11421_RS24905"
                     /old_locus_tag="QE11421_24685"
     CDS             8225..9454
                     /gene="taxC"
                     /locus_tag="QE11421_RS24905"
                     /old_locus_tag="QE11421_24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase/nickase TaxC"
                     /protein_id="WP_001329504.1"
                     /translation="MGVYVEQEYRIKRAKGTGRDPKSPKLSGRHIHASKSAFNHKVRHG
                     ADKKAYTWTPTKEVTFKITGSGKTAAGIKNGIDYITRNGELEAYCYDGKGSEQTGKGED
                     FNREFTSTLSKGNDYSRTYRGENIDHVKNMVFSPPPEAGVSREDALKATVEFLKETYPN
                     HAFVAVYHDDKEDHPHVHVNIKLRDEETGRRLRLTKSETRKFRNGFHRKLKGMGYDVTA
                     TWKKDPERKREIERLQAENPKRLRNVYKVVDFGETSYQNKAGEKRTPFLTYETLKGGKQ
                     VTIWGKDLKNHFESEKLQPGTLIKVKKLAPTLVRSPMFNDDGTVAGYRETHRNNWQIEN
                     IALERNRERQVHERETRQPEESDVKKQLSRKHEQGHNIGFALEHGFTKDSEEHKKLRIQ
                     QERNWKGLGF"
                     /name="taxC"
     gene            9531..9824
                     /locus_tag="QE11421_RS24910"
                     /old_locus_tag="QE11421_24690"
     CDS             9531..9824
                     /locus_tag="QE11421_RS24910"
                     /old_locus_tag="QE11421_24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134733.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO:0016987 - sigma factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_060598542.1"
                     /translation="MTEHDAICISGLHQIFSDEEHLSEQQKDIILMYAYGYTLNEIADF
                     KGLKPSTVRKYLDSVRAELGGVSLAGIRTLVLIRTNALLVSSLSRISERGNL"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            9821..10036
                     /locus_tag="QE11421_RS24915"
                     /old_locus_tag="QE11421_24695"
     CDS             9821..10036
                     /locus_tag="QE11421_RS24915"
                     /old_locus_tag="QE11421_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000760375.1"
                     /translation="MKKYLLFALPFFVVGCSEEVKSVDWWGQHLTEAKQKQAECEKSGS
                     DSQNCKNVKQALFIQSQKDAPVPTFD"
                     /name="EexN family lipoprotein"
     gene            10392..11012
                     /locus_tag="QE11421_RS24920"
                     /old_locus_tag="QE11421_24705"
     CDS             10392..11012
                     /locus_tag="QE11421_RS24920"
                     /old_locus_tag="QE11421_24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_000157549.1"
                     /translation="MASLFPALLLCAASVHPDTINDIARVESGFNPYAVAEIIPERERG
                     SYGKSVISHMPKSKEEALTVIREIENRKRRYSVGLMQITSSNFSFYSTSAEKLLDSCEN
                     LSVFEKIIVDCYKRGRSLENALSCYYTGNFSNGKRKEKEFNNTSYVERIGYTGNEKKYV
                     VPGTRSNGGEQRKNRSHNASVIWPETILKSAFVDNSHPTKVIN"
                     /name="lytic transglycosylase domain-containing protein"
     gene            11026..11352
                     /locus_tag="QE11421_RS24925"
                     /old_locus_tag="QE11421_24710"
     CDS             11026..11352
                     /locus_tag="QE11421_RS24925"
                     /old_locus_tag="QE11421_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000008647.1"
                     /translation="MSFKKSVCNRISSSVTAVMQRKNKVVMAVAGATVASPAFADGFSK
                     AETLLEKVKTGLSGLSLVTVTIACLWVGYKVLFGGSTIRECSPIIIGAIVIASAAEVAS
                     MMVN"
                     /name="TrbC/VirB2 family protein"
     gene            11371..14118
                     /locus_tag="QE11421_RS24930"
                     /old_locus_tag="QE11421_24715"
     CDS             11371..14118
                     /locus_tag="QE11421_RS24930"
                     /old_locus_tag="QE11421_24715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="WP_000105985.1"
                     /translation="MSTLYKAMTRPAMYVGVPVVPLTVVAGALFLAGVYISKLIWLAIP
                     VAVFVLRMITKQDDHIFNLYFLKLKMLGNSVCNRFFGARAFLSGQYEAVEIDEFVNAMK
                     LNERITTGKYIPYSSHVDKNIVKTKNGDYVATWQLMGINFESISAEMLETIDSQVATLV
                     RSFSGLPVSFYNHSCRASFYDAFTTKSGNKYADIISDCYYGSMKKNKFKGNTLYFTLIY
                     RPDGRVEKLEKRKKSIKEKKDDINIHVKRMNEMINTFSGALDKFTCKLLGMYEENGKVF
                     SSQLSFYNYLLTGKLQKIRVTDSPVYNVLGGVDVFFNHDTGQICRIDGNKFFRSIEIKD
                     FCSETASGVFDVLQYSDADYIITHSYTSMSKSEALSTIKRAEKQLKSTEDDAVTQLQEL
                     EKAKNDIVSGDISFGYYHFTLMVMADSIRELDESVSKITADFTDLGIIPALSTMSLPAA
                     YFAQLPAVFHLRPRLSPVSNVNFVELASFHNFYQGKRDKNCWTEAVAILKTPSKQAYYL
                     NLHNSVLFKDETGEKNLANTKVIGTAGSGKTMFLSYLACSLQKYNNPETFADSAKNKKL
                     TCVFLDKDRGAELCIRMLGGEYYTVKSGEPTGWNPFALEATKRNRIFVKQLMEILCTRN
                     GERLSTRERLLISESVDAVMDFPPGEMREYGITRMLEHLMQRDDRDEQENGIILRLSQW
                     ANGQAHGWVFDNAKDTFNIQHVNNFGIDGTEFLDDPMVCAPITFYLLYRITQLLDGRRL
                     VIFLDEFWKWLQDEAFSDFVYNKLKTIRKLNGLVIPATQSPDEILKNKISRAVVEVCST
                     SIYLANPDADYNDYVEGLKLTPEEFNIVKNLDPMSRQFLIKKSSLKKGDGKSFSALATL
                     DLSGLGGYLKILSASADNLEIFESIYHEGMEPDDWVPEYLERAI"
                     /name="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
     gene            14130..14846
                     /locus_tag="QE11421_RS24935"
                     /old_locus_tag="QE11421_24720"
     CDS             14130..14846
                     /locus_tag="QE11421_RS24935"
                     /old_locus_tag="QE11421_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000832283.1"
                     /translation="MKRVKTLMLISLLTASFYSSAGIPVAIDASPEWAVEAARWTERLK
                     QWSETAQHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELTNIYKQGNSYISDFTS
                     NPEGALSSQAKNLFSKYGAFDMCNTGYERNDNLCKARIVSTAASIEQGNEINKQLSSAM
                     SQIQSLSSRIEASKDIKESQDLANALQAQSLKMQAIKMQYDVWNNKNKADHEMLVTQEQ
                     EAFIKQQKEAEPLTFD"
                     /name="type IV secretion system protein"
     gene            14858..15952
                     /locus_tag="QE11421_RS24940"
                     /old_locus_tag="QE11421_24725"
     CDS             14858..15952
                     /locus_tag="QE11421_RS24940"
                     /old_locus_tag="QE11421_24725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677777.1"
                     /GO_process="GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000046889.1"
                     /translation="MAQGFFVKYNSTVMDSVDKISSSYQTQFANDIMSLATVSVTLYVL
                     WKGYQILASKTQTPLQDLVWDLSKFAIIIMFITNADGYLTAATDALQGMKDGFSGGVSV
                     WQTLDNLWKSTQNLGAEIYSLDKSTYVKDQGVVGQFLIWTGSLILMAVSVVVFLTADVT
                     MKLLIITAPIFIFCLMFGFIRSMFNNWLQSLFSSILTVLFASLVIRIAMDFQGMILSHA
                     IRAAQTGNVNLVSTGAMGFMAGFLGALLVLIAKGFAVQLAGAGVEGAVQGAAMMGLGAA
                     GMATGKSLMLGGRAGLGFGLGMAGRTGMNSLSGKAGNLMGRGARTAAEWAGEKGYQALA
                     PTGALGMKARRLASLEKARARNAA"
                     /name="type IV secretion system protein"
     gene            16467..17153
                     /locus_tag="QE11421_RS24945"
                     /old_locus_tag="QE11421_24735"
     CDS             16467..17153
                     /locus_tag="QE11421_RS24945"
                     /old_locus_tag="QE11421_24735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_001290765.1"
                     /translation="MSEKIEKKIETAKSFERQLYAENERSKKNAWRVAVAASLLSLCLG
                     AAICVLVPLKEKELAIVAVGEKTGRTELITQVKQEKILQSEALGRYFVNTYITLREGYN
                     YPSLQYDYETVQLYSSNTVKDDYLRLYNSDMAPDKIYHNNGSYVAVEVISNIISDATAP
                     DKLASVRFKKTTRNFTTGQVSVSYWTARVTYRFEPEKSVKSSSRELNPLGFTVTSYQTD
                     REVRGE"
                     /name="type IV secretion system protein"
     gene            17150..18058
                     /gene="virB9"
                     /locus_tag="QE11421_RS24950"
                     /old_locus_tag="QE11421_24740"
     CDS             17150..18058
                     /gene="virB9"
                     /locus_tag="QE11421_RS24950"
                     /old_locus_tag="QE11421_24740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_001329500.1"
                     /translation="MMKKLFIATCLLLPGLVYSAATPLPSGFDARMQTVSYNGANTTVI
                     RSKTGFLTSVVFDEGEAVISAKAGFPAGWEITTDDNVVYINPRPVVQEQEGDEGEKLKK
                     VFQPTEKEWDTNLFVRTTKRIYSLDLILLSEEKQAQPAYVVQFRYPSEIAKKNAEEVRL
                     AKEKQEKLRQKKLISESFEKADAPKNWNYFMRVNEKYDSRRIAPDFAYDNGIFTFLGFN
                     SGKVFPAPFAVRDGQEQTLAFNVETKGKYKIMVIHNVNDKFVLRYGNSVVGVVNKSFGK
                     VLTDQRNTSSPAVERVEVNND"
                     /name="virB9"
     gene            18051..19169
                     /gene="virB10"
                     /locus_tag="QE11421_RS24955"
                     /old_locus_tag="QE11421_24745"
     CDS             18051..19169
                     /gene="virB10"
                     /locus_tag="QE11421_RS24955"
                     /old_locus_tag="QE11421_24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_000131271.1"
                     /translation="MTDMKKTDDLVDDANQSKLESKPVHDIGDIRNKNNRMRSGFLFGL
                     MAVLVIGIFALKTYKNYFADDQQKASESTGDTSISQVSKIRTGLGQNFDPVENKAIINP
                     GATGSGNTVSDGHKEVPQEGFRKYLSIPVAGQGGSQTGASGSSRTSQTSEPQEERKESK
                     ENVPGKSGMKVTAINLDPDLYIEENRLIPCALTTRFVSDVAGRISCVFTEDVWSANHHT
                     KLLEQGTKAFGRYQTGTLNHGQGRMFVMWEQLRTPDNKRIDMVNTAAAGPLGEAGIDGW
                     IDSHFWERFGGSLMLSMVQDVAAAAADNAPGKDRNVDYTENSRQAMAEMAKVALENSIN
                     IPPTMYKNQGDIITIMVGEDIDFSDIYELKVK"
                     /name="virB10"
     gene            19171..20199
                     /gene="virB11"
                     /locus_tag="QE11421_RS24960"
                     /old_locus_tag="QE11421_24750"
     CDS             19171..20199
                     /gene="virB11"
                     /locus_tag="QE11421_RS24960"
                     /old_locus_tag="QE11421_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401678.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_000017226.1"
                     /translation="MSGVIPVSISNKSLDFYKNQVFQSFLDIEGLTEIAVNRPGEIWTE
                     INGDWTFHNNDSVTFDFCKRFSHTLASFRGDEIGETKPLLSATLESGERVQVVFPPACE
                     RNTISITIRKPSTRQITHKEYVANGFYDYVQSGKKHRTYDDELLDLFRARNIAAFMELA
                     VAAGKTIVFAGATGSGKTTYMKSLIDFIPLNTRLITIEDAEEIKFFIHKNYVHLFYPSE
                     SGSDSGSIITAAKLIKSCLRMKPDRILLAEIKGGDAWDFVKVAGSGHSGSMTSIHAASA
                     KDAIIQMVTKCYQNRECQNLPFDVLRKIIMDSIDIVVHVGRDGVVRHMSDIYYKGAECE
                     NF"
                     /name="virB11"
     gene            20186..22048
                     /gene="taxB"
                     /locus_tag="QE11421_RS24965"
                     /old_locus_tag="QE11421_24755"
     CDS             20186..22048
                     /gene="taxB"
                     /locus_tag="QE11421_RS24965"
                     /old_locus_tag="QE11421_24755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxB"
                     /protein_id="WP_170324155.1"
                     /translation="MKTFNKSQSVFIFAVMLVAAWFAGSFIFFVLYFSQIKNLKPLKAV
                     FRSIDAYRMDIFTDSLTLSVVTTDIRVLAFVALGAGFVVSLIIPVAALIKLNEKKENIF
                     GDARFATINDIRESNSFTLDGDEKDGIIVGIKDKKIIRYVGAAFSAMGAGTRAGKGAGI
                     VITNLMKYWWSVIILDPKRECFNITSLIRKVILGHEVYKFDPFSSVTHRFNPLYYVSMG
                     TSEGFNQLENLSLIIYPYKTDGADAGSYLNKTAGGVFKSYAVALWFMIKNDKAGLKTLD
                     IEPVFSMSKINQLFERAEPEHLLSFMNDIRSELRGKDKTLADIGVAGLKAFIEMEDKTK
                     AQLKSNFLNGLSPFANPNVANATDGNDFDLRQVRKKRMTIYFCISGDNARLAEKITNIF
                     FQLAIQVNLEKMPTDDPEIKHDCLFLLDEFPSIGAVDYIKSKSGLIAGYKLKLLIVYQV
                     GSQLEEIYSYAGSKTLLASAPCKIVYSASDVKDARELSEAMGTRTVTIGSKSKSRSRGG
                     TSRSESESLIERPLVTTNELLTLKFSEEILAMKGENPIRCEKAFYYLNEYFFGDFVKVA
                     PELANIFPRKKGKLVMPPQKVFENEIVAKGYLVVKDVPDLDKAA"
                     /name="taxB"
     gene            22057..22479
                     /locus_tag="QE11421_RS24970"
                     /old_locus_tag="QE11421_24760"
     CDS             22057..22479
                     /locus_tag="QE11421_RS24970"
                     /old_locus_tag="QE11421_24760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_015059903.1"
                     /translation="MMKKLVFLLISILAGCSSPPEPTPVQFEKANEVINPSLPYVPDFH
                     GVIKSDVSGKGWVYEITSLSGVQARTPTFYYALAHADRIVVTTHDAGLWFRIRDLLKME
                     GATAVVEWRNEKSFLPEQVKIVFIKAQNEVKNDWKK"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            22463..22768
                     /locus_tag="QE11421_RS24975"
                     /old_locus_tag="QE11421_24765"
     CDS             22463..22768
                     /locus_tag="QE11421_RS24975"
                     /old_locus_tag="QE11421_24765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_001350475.1"
                     /translation="MTGKNRVVLPALLALGLISTNANASDPCASVLCLYGKAVGQGGGS
                     ECRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVRG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            <22881..23075
                     /locus_tag="QE11421_RS24980"
                     /pseudo=""
     CDS             <22881..23075
                     /locus_tag="QE11421_RS24980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /name="ATP-binding protein"
     gene            23079..23651
                     /locus_tag="QE11421_RS24985"
                     /old_locus_tag="QE11421_24770"
     CDS             23079..23651
                     /locus_tag="QE11421_RS24985"
                     /old_locus_tag="QE11421_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001198960.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKENPVKELPDIDESAHPFH
                     ICYGGYLLNPGVSDSDISRKIKDQAGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGHQK
                     IVPFAALIKADECIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCERRFRV
                     VSKDNWQHTVSEERAIRCAKRLIQCKG"
                     /name="hypothetical protein"
     gene            23656..25809
                     /locus_tag="QE11421_RS24990"
                     /old_locus_tag="QE11421_24775"
     CDS             23656..25809
                     /locus_tag="QE11421_RS24990"
                     /old_locus_tag="QE11421_24775"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="WP_001235745.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHII
                     ESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHAGD
                     PDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKALARS
                     VADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFYYTMT
                     GVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDDKKTAA
                     PLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQFSEAPQV
                     IDALKSVFPQSLDIDSARKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERNVYLAIAQ
                     HYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGESEDNDDSA
                     FELLCKIRTGETLTTKEVIVNEKKTTPPPLFTEASLLAALVRVADFVTDPTIKKLLKDK
                     DKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDALPGIAVNPDM
                     TALWSEKQAAIENGDLTVEQFINDLYGELTGMISDVDLGEMKIEPAAPAGQFQRLDSPC
                     PSCGKHIVIRPKGYFCTGCEFKIWSEFSGKKITQAQAEKLVKSGKTDLIKGFKKKSGGT
                     YDTVLVLEDKKTGKLGFPARAKK"
                     /name="type IA DNA topoisomerase"
     gene            25806..26012
                     /gene="hha"
                     /locus_tag="QE11421_RS24995"
                     /old_locus_tag="QE11421_24780"
     CDS             25806..26012
                     /gene="hha"
                     /locus_tag="QE11421_RS24995"
                     /old_locus_tag="QE11421_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="WP_000850859.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAADH
                     RRAELVMNKLYDKVPSGVWKYVH"
                     /name="hha"
     gene            26030..26494
                     /locus_tag="QE11421_RS25000"
                     /old_locus_tag="QE11421_24785"
     CDS             26030..26494
                     /locus_tag="QE11421_RS25000"
                     /old_locus_tag="QE11421_24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293133.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0030527 - structural constituent of chromatin [Evidence
                     IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="WP_001293134.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREIDFEQLLEMQEKLNVV
                     IEERREDAEREAAERQELEAKRQQAIEYIISLGLDPESLLAPVTADTVKTRRKAKGGVR
                     KAKYRFKDENGEIREWSGNGKRPLALQKLLDEGHFMEDFLIEKTKPEQAE"
                     /name="H-NS family nucleoid-associated regulatory protein"
     gene            26581..27285
                     /locus_tag="QE11421_RS25005"
                     /old_locus_tag="QE11421_24790"
     CDS             26581..27285
                     /locus_tag="QE11421_RS25005"
                     /old_locus_tag="QE11421_24790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            28068..28163
                     /gene="dqlB"
                     /locus_tag="QE11421_RS25520"
     CDS             28068..28163
                     /gene="dqlB"
                     /locus_tag="QE11421_RS25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_021534057.1"
                     /translation="MINMLIIVLRAVVAVANALIAVLELIRQFID"
                     /name="dqlB"
     gene            28826..29473
                     /locus_tag="QE11421_RS25010"
                     /old_locus_tag="QE11421_24800"
     CDS             28826..29473
                     /locus_tag="QE11421_RS25010"
                     /old_locus_tag="QE11421_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000587671.1"
                     /translation="MIIVIGGDKGGTGKSHLATNLTVCLSQQGKRTGLVETDLNGSTKK
                     WNKRREQAGLPPVALNEAYGDISAKITKMADVAEILIIDTAGYDSTEFRTALKVADIVI
                     VPIDPLAQVEADSLQTVTKIVRDAQKINPRLAAHVLLYKCQPNTFSEQQELRDSLNSHD
                     YWLKPMKSTVSFLRAFVRAMNQGMGVHELKSNVSGASQAKAQIELLLKELEL"
                     /name="division plane positioning ATPase MipZ"
     gene            29548..29817
                     /locus_tag="QE11421_RS25015"
                     /old_locus_tag="QE11421_24805"
     CDS             29548..29817
                     /locus_tag="QE11421_RS25015"
                     /old_locus_tag="QE11421_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001086090.1"
                     /translation="MAKLELPELEQNQEVNATKFVQDAGKRPTENKTKLASFRIPNELL
                     EFIDNESKRLHRPKTKIIKAALLAYQDLDENELINLWFRADRND"
                     /name="hypothetical protein"
     gene            29978..30607
                     /locus_tag="QE11421_RS25020"
                     /old_locus_tag="QE11421_24810"
     CDS             29978..30607
                     /locus_tag="QE11421_RS25020"
                     /old_locus_tag="QE11421_24810"
                     /inference="COORDINATES: protein motif:HMM:NF022440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2806 domain-containing protein"
                     /protein_id="WP_223424520.1"
                     /translation="MSLIDLSLSGLSEPGTKLIEKISDAIGVLYEPTRIRKKAKAEAEA
                     KRTELISRLELEGIEKRAVERFLKRETKRQENIENITMQAAQSLSESDNVSDIDEDWIE
                     AFFRECEDISDEQMQMLWGRILSEEAKSKGSFSRRTLKLLSTISKEEANLITYFGKFVW
                     QANKLTPILFTDENGDTEGITFDKLSVLDSLGVIQQGIGYSLRYKL"
                     /name="DUF2806 domain-containing protein"
     gene            30781..32406
                     /locus_tag="QE11421_RS25025"
                     /old_locus_tag="QE11421_24815"
     CDS             30781..32406
                     /locus_tag="QE11421_RS25025"
                     /old_locus_tag="QE11421_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_049589868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase MCR-1.1"
                     /protein_id="WP_049589868.1"
                     /translation="MMQHTSVWYRRSVSPFVLVASVAVFLTATANLTFFDKISQTYPIA
                     DNLGFVLTIAVVLFGAMLLITTLLSSYRYVLKPVLILLLIMGAVTSYFTDTYGTVYDTT
                     MLQNALQTDQAETKDLLNAAFIMRIIGLGVLPSLLVAFVKVDYPTWGKGLMRRLGLIVA
                     SLALILLPVVAFSSHYASFFRVHKPLRSYVNPIMPIYSVGKLASIEYKKASAPKDTIYH
                     AKDAVQATKPDMRKPRLVVFVVGETARADHVSFNGYERDTFPQLAKIDGVTNFSNVTSC
                     GTSTAYSVPCMFSYLGADEYDVDTAKYQENVLDTLDRLGVSILWRDNNSDSKGVMDKLP
                     KAQFADYKSATNNAICNTNPYNECRDVGMLVGLDDFVAANNGKDMLIMLHQMGNHGPAY
                     FKRYDEKFAKFTPVCEGNELAKCEHQSLINAYDNALLATDDFIAQSIQWLQTHSNAYDV
                     SMLYVSDHGESLGENGVYLHGMPNAFAPKEQRSVPAFFWTDKQTGITPMATDTVLTHDA
                     ITPTLLKLFDVTADKVKDRTAFIR"
                     /name="phosphoethanolamine--lipid A transferase MCR-1.1"
     gene            32478..33200
                     /locus_tag="QE11421_RS25030"
                     /old_locus_tag="QE11421_24820"
     CDS             32478..33200
                     /locus_tag="QE11421_RS25030"
                     /old_locus_tag="QE11421_24820"
                     /inference="COORDINATES: protein motif:HMM:NF028536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family protein"
                     /protein_id="WP_039003037.1"
                     /translation="MPYLSNKRLLAEMSIALVMAIVATLTLEHSQIDLMVADWFYLGMG
                     HWMVAKQAFLPDLLLYSGLKKLLMAMLIYLLVATICRAYHEKKGNAITAKWLVPVTKFR
                     VRELAYLVLTLILVPTVVASLKAYTHVVCPVHLTIFDGTLPYLPMLDSMRNTIPDKCFP
                     AAHASSGFALFAFAFAPSLRRRRGAIIIVVMALGWAMGCYKMIIGDHFLSHTVVSMMLA
                     WAMSAGLAWVFFKKGEQV"
                     /name="PAP2 family protein"
     ARG             30782..32403
                     /name="mcr-1.1"
                     /gene="mcr-1.1"
                     /gene_name="phosphoethanolamine--lipid A transferase
                     MCR-1.1"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(1362..2233)
                     /name="IncX4"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8226..9451
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.511"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          14859..15925
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.719"
                     /coverage="99.0"
                     /evalue="2.69e-172"
     Typing          16468..17150
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.4399999999999998e-137"
     Typing          17151..18055
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.669"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          20187..22045
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.71"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acttacgcct atcggtcaag aattgatgaa aatatgcggt tccactccag atattgcgta
       61 tttaaataaa ttcctcaaca aaataaatgt tgaaggaagc caggtaaaac tatcaattat
      121 taatatgtaa agaggtcgag ttacctgtct gagttccact aaccataatt aaaagcgacc
      181 gcaaaaactg cccagtgaaa actgtgttgc cagtaatcac aaatgcagca agtactaata
      241 aagagcctgt taagcccgca aatatgtggg cttattttta attactcttc atcatcccgc
      301 aggaactgac aaaaatcttc gctccccatc atatcgacag tgtttttaaa ttcgaaaaaa
      361 gaaattcctg tcatagcaca cacgcgctgg ataatcagtt ttttcttttg ctgtctgacg
      421 gttgcggttt caccaaactg gataacagcc aggactgttt catcatatga tttcaggaca
      481 aaatcacagg ttatattctg cacttcacgg attaaatctt ttacagcttc ctcgctacca
      541 tgaggacgaa caaattcaat gagccgtatt ttgggaaaaa cggaataatc aggtataata
      601 ttacgtgaaa gaatgcctaa aaactctgat tcattatttg agagtaaggg tactttagta
      661 aaacaatcaa cctccccaat ggctgtaaaa gcattggttg tcaaagctgg attgttctta
      721 ataaaatcag agaatacatt ttcagctgat ggcaaaacaa cagcagaatt atgttgttca
      781 tctcgttttt tattacggtt aaatatcatt tcatattcct tttaaaagag cctgcggcag
      841 gcacaggcaa aaacatcgtt tttggacaat ctgattcaaa tgttaacatt tgacatcgga
      901 ttgtctatcc tgcattgttc tttactgtct gtaagatcgg cgctcattct tccggcgttc
      961 tcccgatacc aaaaagacgg aacgcaaaac ggctttcgat ccaccattca aaaatctgct
     1021 ctgcgcaacc acaaacagtt aaggcgatcc atgcagcaag aacaacgtaa ctactgacat
     1081 ctgcacccat gtaccaggcg atagttaaac ccatcactgc gataccgcct aaaaactgca
     1141 acaagaaacg aaatcccgca agaatcccca atggaataac aacaaccaga taaagggaag
     1201 cagccagaag gtatctgaca ggtgtcagca gataaaagaa cctggatatt ttctttttgt
     1261 cagaataaac ggcatcattt gcaacatcgg ttgaatgatc attcatgctg tccctgattc
     1321 tttgaaaatc tacgatatta tccttcatat tgttatgacc tcaattttca ggtgggtaca
     1381 ttttatcaaa catattagta aactctgagt cttccctggt atcgccctca taaccaattg
     1441 tataagagaa ctttaattta taaacttttc tcccaatttt ttctgaaact acgaaactta
     1501 agttctttac ttccgtatgt ttcatgattt cttttactga tttattaaga acatctcttt
     1561 taaaagcagg ataatctgga tacttataat ccttcacccc ccccgcccct attgtataaa
     1621 gattcaattc atctttaagt tcatctattg tcatttcaaa actattcgcg cgagaattat
     1681 tactatatat tttacgaatg agttgatata gtgatgcagc attaacactt gtcagtcgaa
     1741 caacagataa taaaacttgc gttgtataac gtttttcttc accaataagt ttacataaaa
     1801 atcttttcgc ttctttcgta aacttgatcc ccactctacc ttcattcctg taataagtag
     1861 aaaaaacagt cagactcaaa ttcattgaat caggtacatt acttttagtg aacttaaatc
     1921 ccaaatcact actcagttct aatatttcat catgtttcag cttaagaagc gtcgtatcta
     1981 atatattaga gccatccctc aaagcaagat aagccgcatc aggcttaacc tgaacccatt
     2041 taatgtaatc ggcaactgtc acataaaata tgtgatcatc atcgaattca tttttagagt
     2101 ttatctggca catggctaaa aataagactt ttttagcagc cataggcaat gttgacagcg
     2161 ttgaattcaa ttcatttcga tgtctcactt ttgttaagcg agaaagggaa gtcttatttg
     2221 tcgtcattct cattgttcca acactcttga taatcagact ttaacaccag aaacatgata
     2281 ttacaacaca atcaatatca tgtttaaccg agtatcttct catgtttcat atctgtgatt
     2341 tatctcacaa aggtaatcca tgctcaaaat ccacactgga ttcaaggcag attaacaaca
     2401 gattcaaaat agattcacca gaaagaacaa cgaattagta ttcttatgac acattttgtg
     2461 gatatcaccg aggaagctct catgtttaac cgaggaagct ctcatgttta accgaggaag
     2521 ctctcatgtt taaccgagga agctctcatg tttaaccgag gaagttatca tgtttaaccg
     2581 aggaagctct catgtttaga caaaaaacac gcttaaaatc aataaattag agctactcta
     2641 agtttttaag tttttaagaa aaacaagaaa agataaggct tttaatgcgt gcgaatattc
     2701 aggaactgtg gataactcaa caaaaccagc aacgatgatg gttccttacc ataaatctgt
     2761 cccctgatga ttattacccg caagctaaaa caaacatcat caatcattaa aactcataac
     2821 tttcaataac ataaaatcat caaaaaaata tttgatgcct tatgtatctc aattttcaag
     2881 accataaatt tgtccctagt taactttaac ttattgaaat aaatataata ttcgtgcgta
     2941 ctgatcctaa tgtcacatcg caaaaattcc gcttcctcgc tcactcagtc gctacgctcc
     3001 tgccgttcgg ctgcggcgag cggtatctcc tttctcaaaa ccagagagat tcagatcata
     3061 tgatccgtta tttgatcctt tttggatctt ccactaagaa gccctgaatc gcgctctgcc
     3121 gcttcgttcg atacaaccat gaggaaaacc atgcgaaatg aaaaaaatcg cttaaaatgc
     3181 gattcagagc gttttaggga ggttctgtta gttctcctgc attgcgcccg tagttcgcta
     3241 acgctcacac gcgcagtaca gcgccccgcc atgctgcaat gcaccagggc ggggtaaact
     3301 cgcttgcgct cgcgctaact gcggcattct gcggcgcaaa tcagcataat catggcctgc
     3361 tggtcagttg ggctttgcag ggtgaaggga tggcctgtgc accctggtac gttcgtcagc
     3421 ggctgcgccg cgcttcgggg cgatgtggct cctgtgctgc cctggtattt tcgttcgccc
     3481 cttccatccg cttacgcgga ttaaaaaacg tgataacaat cacttatcga gtaaaaaaac
     3541 gggagaaaac ttgcaaatac gacttaaagg tcgtattatt gtttcatcga aaggggaatg
     3601 gcccctctca caatagccca gcagggcagg agattaaaaa aatgaatatc caggaagcat
     3661 taaacgtttt tggattatcc ggcgaattaa ctgaaaaaga tatcaaagca gcatacagaa
     3721 aagccgcttt aaaatatcat ccagatcgta acccgttagg ggctgaactg atgaaagcgg
     3781 taaatgcagc ttttgatgta ttgatggcaa atattgataa aataaatcag ttccagagca
     3841 ctgatgaaca tgcacgatat aattacggtg atgacctgga aaaagtatta aacgttcttt
     3901 ctggtttatc tggtctggta tttgaagtga taggtaactg ggtctggatt agtggagaaa
     3961 ccattacaca taaggaaact ttaaaagaaa tcgggtgtaa atgggcggca aagaaaaaac
     4021 aatggtttta tcgtccagac gaacataaaa gttactggaa tcgtgaagaa cacacgatag
     4081 aagaaatccg cgcaaaatac ggtacaaccg gacagcgcag ggcgacaggg tggcaacgcg
     4141 tggaaaccag agcgtaacca gaacgggggc gagaagcccc cgatattata gccagcccgt
     4201 cttttggggc atcgaaccca aaaagacggg aacagcagcc acgtagcaaa aagggaaaca
     4261 gatgaactct ttttttgaac aatatcaccc tgtctttgaa gttgtctgcc gtatcctggg
     4321 gaacggctgg cgcgtgaaca aacttgatga ttgttcgtct cgtataaaac tgacgtcacc
     4381 gcagtttaaa aattactctg tgcatattcg gatggaaaaa gaccgctttt ctgtggtggg
     4441 gagtgtggat agtcgttcct ggcgtagtcc gcatcatgtt tgcacgttat ccagaaaacg
     4501 gaatcccgtt gatattgcag ccgatatcga gcgaaaaatt ctggtaaatg cttcgcagga
     4561 ggtgttacag gcgattgagt atgagaaaca ccaggtggaa aaaaaggacg aaattctgat
     4621 cctgaaaggt atgttatcgc aacttgtcca gcttgaaagc tggtatgggg cattgacagg
     4681 ctttaaagct gaaaacggat tgaacggtaa agtcaccgaa cagggcgaca gttatgattt
     4741 acagattcgg ggcttgagta tagatcaact tgttaaaatt acagggtatt tgaaacagtt
     4801 atgagaaaaa ataaaggtga tgtgacttat tttcttgaaa aagaaggtga caattacagg
     4861 cttacaaaaa ggataaaagc cagaacaaat gttaaaatcg ggaacaaaac tacgaaaatt
     4921 acattgtatg atgctgtact gaatgagaat gaattacaac atattgattt tacgtgtgcc
     4981 ggattaaggg aagacgatga gacgccagtt aagaatttaa ttaaggagtt tatgttaaat
     5041 gaaacccgat aatacacctg aaaatgtaaa aaaattacgt ttaaaagcag gattaacaca
     5101 aaaggaatgc agtcatattt atggtgttgg gctaagaacc tggcagaaaa aagaggaagt
     5161 taatactcaa aacagtcaaa gtctgtcact ggttgagttt gagtttcttt tgttacttgc
     5221 aggagaacat cctgaatacg tgctctgcaa aagggagtca aaataaaatg acctgtgatg
     5281 gttttatatt agtacctgga gttgcgctcg tagctcgctg acgctcacac gcgcagtaca
     5341 gcgccccgcc atgctgcaat gcaccagggc ggggtaaact cgcttgcgct cgcgctaact
     5401 gcggcattct gcggcgcaaa tcagcataat catggcctgc tggtcagttg ggctttgcag
     5461 ggtgaaggga tggcctgtgc accctggtac gttcgtcagc ggctgcgccg cgcttcgggg
     5521 cgatgtggct cctgtgctgc cctggtattt tcgttcgccc cttccatccg cttacgcgga
     5581 ttaaaaaacg tgataacaat cacttatcga gtaaaaaaac gggagaaaac ttgcaaatac
     5641 gacttaaagg tcgtattatt gtttcatcga aaggggaatg gcccctctca caatagccca
     5701 gcagggcagg agatttaaaa tgaaatgctc atctgttttt acttcaacca ctaatcatgt
     5761 tttcacattc gaacgagtaa cgctttgcac tattatcctt atgcataagg atactggaca
     5821 acagtatgtt gtcatattta ctgataacaa taaaattcgt gactataaag ccggaattgt
     5881 tcctcagttt ggcgaactta agcagagtga tgttgattta gtccttttct acagggacga
     5941 atatgaaaaa tattttgatt cattaaaaga tggcgatgaa tgcttgagtt ttaaagattt
     6001 tattgagtgt ctttgttaag cattatgaat gcaggggcat ccctgcattt tctggaaatt
     6061 tcaatattga ttctgaaatt gtctattaac atcaatgcaa gggaaattca tgaaaacgtt
     6121 aaaacaagcc gcaatgcagt ttgcatctga tctgcgtaat aatcgctgtt ttaaagctgc
     6181 ttataatgat gctctgttag agcttgatcg ggaagattta aacgccattg tacaatgcac
     6241 atttcttttt gagccaaaac gaacattagc cgaacttgat aaattaatta aaaagataga
     6301 agtagaagaa gattatgagt tttgtgtatc ccttgataaa ggtaaaggat tacacgaagt
     6361 cagaacattt aaaagtgagc gtgaggcgtt ggatgcttat cgcattttga ctctttacgg
     6421 atacacagta actatagaaa agaaggaggg taatgactga ctattcaagg tgtgatcagg
     6481 ttgttccacc aacctgatca cgttatcgac aatcaaaact gaatgaggtt tttaaatgtc
     6541 aacgcggcag atcgtacagc ttgctgattc ccttgtcaaa ggctgggaaa ccagaattcc
     6601 ggcaatgaaa tttagcgaat gggatcaatt ccggtggtgg ttaaactact tgcaagggta
     6661 tgaaatgttt taaataaaaa aaggggcttt ctgccccttt gtattttttg acaaaaatgc
     6721 catgtgtaac tataattagt tgcatatcta taggggatgg atttatgggg aaaacagata
     6781 agctactggc aaagttttta aacagtaaaa aaacgtttga atgggatgaa ctggtcgttt
     6841 tgttttcctc tttgggatat gtcaaaaagg aaatgcaggg gtcaagagtg cggtttttca
     6901 atgctgaaat caatcacacc atattaatgc atcgtccaca tccggaaagt tatatcaaag
     6961 gtggtacgct gaaagcaatt aaacagaatc tgaaagaggc tggattatta tgaaacattt
     7021 aaaatataaa ggatatttag gcacggttga acccgatttt gaaaataatg tcctgtatgg
     7081 aaaactggcg tttattcggg acctggtaac ttatgaggct tctacattag ctgaactgga
     7141 acaggagttt aaaacatctg ttgatctgta tttacagtct tgtgtggagg atggaaagga
     7201 acctgatacc ccgtttaaag gcgtgtttaa cgtcaggctt gatccagaac tgcatcgtcg
     7261 ggtagcagaa atggcgatgg aagaagattt atcgttgaat gcctttgtta ataaggctct
     7321 ggaaaaagaa gtcagcaatc atcgcgcagg ggcttaattg cccttgtttt tatgccctgt
     7381 aagggcatgg gaggcgcttt gcgccggaag ccccgcagcg cagcgaggac ttaaaaagcg
     7441 ttacggcttc agccgtaaca attcccagcc ctgccaggcc tgttttttct ttcacaaatc
     7501 tttcagctat acgtttgcct gatggtcgaa gacgaaagaa tctttcagca acgaaatctg
     7561 gacatatgca gcgacgattt tatagccagg cacagtgcca catatggtac aatcaatgca
     7621 ggatagacaa tccgatgtca aatgttaaca tttgaatcag attgtccaaa aacgatgttt
     7681 ttgcctgtgc ctgccgcagg ctctgttggg gtgacttatg aagatgatag cgttcaggct
     7741 aagtgatgat gagttaaaat ttgcagaaca taacgctatt ttaagtggat ttaccagtat
     7801 caatgctttt gctaagcata acgtgcttaa tattgaaacc aagcctgtta acatcccagt
     7861 taataatgaa ccagcaaagc tggtatcagt gcgtctttac cctcatgaaa tagaacttgt
     7921 taaacgtaat gctgctcttc acggcatgag catgagcaga gaaattgcta ttcgtgtgcg
     7981 tcagtctttg ttaaagagtg aagtctgtct ttaccctgat gaagttaagg aacttaaaaa
     8041 actatcaact gctgtagatc gtgttggtcg caacattcat tttatcataa aaggtgaaag
     8101 attctgtacg gttaacgatc cagatttcag aaaagacgtt attgaagtga ttgaactgtg
     8161 taagcaaatt gattcaaaac ttgaaacgtt aaccaagagt gttgttaaca gatttgggtg
     8221 atttatgggt gtgtatgtag aacaggaata ccgtattaaa cgcgctaaag ggactgggcg
     8281 tgatccgaaa tctcctaagt tatcgggaag acatatacat gccagtaaat cggcttttaa
     8341 tcataaagtc agacatggtg cggacaagaa agcatatacc tggacaccta caaaagaagt
     8401 cacttttaaa atcactggct caggtaaaac agccgcaggc atcaaaaatg gtattgatta
     8461 cattacccgt aatggtgaac ttgaggctta ctgttatgat ggaaaaggtt cagagcaaac
     8521 agggaaagga gaagacttta atagggagtt tacttcaact ttatctaaag gaaatgatta
     8581 ctccaggaca tatcgtggag aaaacatcga tcatgtgaaa aacatggtct tttcaccacc
     8641 accagaagct ggagtaagca gggaagacgc tctgaaagct acggtagaat ttcttaaaga
     8701 gacttatcca aaccatgctt tcgttgccgt ataccatgat gataaagaag atcatcctca
     8761 tgtacatgtt aatattaaac ttcgtgatga agaaacagga agaagattaa gacttactaa
     8821 atctgaaacc aggaagttca gaaacggttt tcatagaaaa ctgaaaggta tgggatatga
     8881 tgttaccgct acctggaaaa aagatcccga acgtaaacgg gagattgagc gattacaggc
     8941 tgaaaatccg aaaagactcc ggaatgttta caaggtggtg gattttgggg agacttcata
     9001 tcagaataag gcaggggaaa aacgaacacc tttcctcact tatgaaactt taaaaggtgg
     9061 aaaacaggtt acgatttggg gtaaagacct gaaaaatcat ttcgagtctg aaaagctcca
     9121 gccaggaacg cttatcaaag taaaaaaact ggctcctacg cttgtacgta gtccaatgtt
     9181 taatgatgat ggaactgttg caggctacag ggaaacgcat cgcaataact ggcagattga
     9241 aaatattgcc cttgagcgga acagagaacg tcaggttcat gagcgtgaaa ccaggcagcc
     9301 ggaagagagc gacgttaaaa agcagctatc cagaaaacat gagcaaggtc acaatatcgg
     9361 gtttgctctt gaacacgggt ttacaaagga ttcagaagaa cataaaaaac taagaataca
     9421 gcaagagagg aactggaaag gcctgggatt ttaatttgat tttggcgcag gtgaacaaag
     9481 cggctacttt gctcacctgc tgatcacaac aacctacgag ggaggttatc atgactgagc
     9541 atgatgctat ctgcatttct ggacttcatc aaatttttag tgatgaagaa catttgagtg
     9601 agcaacaaaa agacattatt ctgatgtacg cttatggcta taccctgaat gaaattgctg
     9661 atttcaaagg actaaagcct tcaactgtca gaaaatatct cgattctgta agagctgaac
     9721 tgggaggtgt ctcgttggca gggatcagaa cactggtact cattcggact aatgcccttc
     9781 tcgtgtcaag tctttccagg atcagtgaga gggggaattt atgaaaaaat atctgctttt
     9841 tgctttgccg ttcttcgttg ttggttgtag tgaagaagta aaaagtgtgg attggtgggg
     9901 gcagcatctt acggaagcta aacaaaagca ggcagagtgt gagaagtctg gctctgattc
     9961 tcagaactgt aagaatgtta aacaagcatt gtttattcag tctcagaagg atgctccagt
    10021 ccctacattc gattaactaa tgtttcaatc atactagagc gcccatatgg gcgctttttt
    10081 ttgcatgatc acacgaatca taatatgacc attttgtcaa tgttatttga tcgtattgtt
    10141 gagcattgtt attttgcgaa tcatgttcgc atctaaaaca aatccaaaac acacttatag
    10201 tggattgatt aaatgaagga gaaatcagat aagtcatcgg aaaaaggggt ttatgtcacg
    10261 ataaaactcg atcccaggtt gaatgcattt atcgaagaga aaacgaaaac atctttgctt
    10321 agtaagagga agcaaattat ttacttatta atgcagagta tgcgtattga tggatatatg
    10381 gagcgttagc tatggcgtct ttattcccag ccttgttgct gtgtgcggcg tcagttcatc
    10441 cggatacgat aaatgatatt gcacgggtcg agagtggctt caatccttat gctgtggctg
    10501 aaattattcc tgaaagggaa cgcggtagct atggcaaaag tgttatcagt catatgccca
    10561 aaagtaagga ggaagctcta acagttatca gagaaattga aaacagaaaa agacgttatt
    10621 ctgtcggatt gatgcagatt accagcagta atttttcttt ttattccacg tccgcagaaa
    10681 aactccttga ttcgtgtgaa aacttgtctg ttttcgaaaa gattatcgtg gattgttata
    10741 aacgagggag gagcctggag aatgcgctta gttgttacta tacaggaaat tttagtaacg
    10801 gtaagcgaaa agaaaaagaa tttaataata caagttatgt tgagcgaatt ggttatacag
    10861 ggaacgaaaa aaaatatgtt gtaccaggta caagaagcaa cgggggagaa cagcgaaaaa
    10921 atcgttcaca caatgcgtca gttatctggc ccgaaaccat tctgaaatct gcttttgttg
    10981 ataactcaca tccaacaaaa gttattaatt aacaggactt ataaaatgtc atttaagaaa
    11041 tcagtatgta atcgtatcag ttcatctgtc actgctgtaa tgcagcgtaa aaataaggta
    11101 gttatggcag tcgccggggc aacagttgca tcaccggcct ttgcggatgg attttcaaaa
    11161 gcggaaacac tgctggaaaa ggttaaaaca gggcttagtg gcctttcact ggtcacggtg
    11221 actattgcct gtctgtgggt cggctacaag gttctgtttg gtggaagcac gattcgtgaa
    11281 tgctcaccta tcatcatcgg ggcaattgtt atcgccagtg cggctgaagt tgcatcaatg
    11341 atggttaact aataaagcgg ggacgatagt atgtctacac tttataaagc gatgacacgc
    11401 cctgctatgt atgtgggtgt tcctgtcgtc cctcttactg tggttgcagg ggcgttattt
    11461 ctggcggggg tttatatcag caagctgatc tggctggcta ttcctgtcgc ggtttttgtt
    11521 ctcagaatga tcactaaaca ggacgaccac atttttaacc tttactttct taagctgaaa
    11581 atgctcggaa actcagtgtg taatcgtttt tttggcgcac gagcgttcct ctccggacaa
    11641 tatgaagctg ttgaaattga tgagtttgtt aatgccatga aactgaatga gcgaatcact
    11701 actggtaaat atatacccta ttcaagccat gttgataaaa atattgtcaa aacaaagaac
    11761 ggtgattatg tagcgacatg gcaactgatg gggattaact ttgagtcaat ctctgcggag
    11821 atgttagaaa ctattgactc acaggttgcc actcttgttc gttcattttc cggactgccc
    11881 gtttcatttt ataaccattc atgcagggcg agtttttatg atgcctttac cacaaaatca
    11941 ggtaataaat atgctgatat aatatcagat tgttactatg gttcgatgaa gaaaaataaa
    12001 ttcaagggga acactcttta ctttacgctg atctacagac cagatgggcg tgtagaaaaa
    12061 ctggaaaaac gcaaaaaaag tattaaagaa aagaaagatg atattaacat tcatgtgaag
    12121 agaatgaatg aaatgattaa tacattctct ggcgctcttg ataaatttac ctgtaaactt
    12181 cttggtatgt atgaggagaa cggaaaggta ttttcttctc aactgtcatt ctataattat
    12241 cttctgacag ggaagttgca aaaaatcaga gtcactgatt ctccggtgta taacgttctt
    12301 ggtggagtgg atgtattttt taatcatgat accggacaga tatgccgcat tgatggaaat
    12361 aaattctttc gttctattga aattaaagat ttctgtagtg agactgcatc cggtgttttt
    12421 gatgtgctcc agtattctga tgctgactat attattacgc attcatatac atccatgagc
    12481 aaatcagaag cattgagtac cattaaacgt gcggaaaaac agttaaagag tacagaagat
    12541 gatgcggtta ctcaattgca ggaactggaa aaggcaaaaa atgatattgt gtccggtgat
    12601 atatcctttg gttactacca tttcacgcta atggtaatgg cggacagtat cagggagctt
    12661 gacgaatctg taagtaaaat tacggcagac tttactgacc tgggcattat tccggcattg
    12721 tcaaccatgt cattgccagc ggcatatttt gcccaacttc cggcggtttt tcatttaagg
    12781 ccgcgtttat ctccggtatc gaatgtaaat tttgttgaac tggcatcatt ccataatttc
    12841 taccagggga aacgcgataa aaactgctgg acagaagcgg ttgctattct caaaacacca
    12901 agcaaacagg cgtattatct gaatttacat aactcagtac tgtttaaaga tgaaacaggt
    12961 gaaaagaatc ttgctaatac taaagttata ggaacggcgg gttcaggtaa gacaatgttt
    13021 ctgtcatatc ttgcctgttc attacagaaa tacaataacc ccgaaacttt tgctgattct
    13081 gcaaaaaata agaagttaac gtgtgtcttt cttgataagg acagaggggc tgaattatgt
    13141 atccgaatgc ttggtgggga atattacacc gtaaaaagcg gtgagccgac aggatggaat
    13201 ccttttgctc tggaggcaac aaaacgtaac cgtatttttg tcaagcaact gatggagatt
    13261 ctgtgtacca gaaatggtga aagattatcg acaagagaaa gattgcttat tagcgaaagt
    13321 gttgatgctg tgatggattt tcctcctggc gaaatgcggg aatatggcat taccagaatg
    13381 cttgagcatc ttatgcagag agatgatcgg gatgagcagg agaacgggat tatccttcgt
    13441 ttgtcccaat gggctaatgg tcaggctcat ggctgggttt ttgataatgc taaagacaca
    13501 ttcaatattc agcatgtcaa taattttggc attgatggca ccgagtttct tgatgatccg
    13561 atggtatgtg caccaattac tttctatctg ctgtatcgca ttacgcagct tctggacggt
    13621 cggcgtcttg ttatcttcct ggatgagttc tggaaatggc tacaggatga agcattcagc
    13681 gactttgtct ataacaagct gaaaacaatt cgtaagctga acgggctggt tattccggca
    13741 actcagtctc cggatgaaat actgaagaat aagatttcac gggctgtggt tgaagtatgc
    13801 agcaccagta tttatctggc taaccctgat gccgattaca atgattatgt ggaagggctt
    13861 aagctgacac cagaagaatt taatattgtt aaaaatcttg atccaatgtc gagacagttc
    13921 ctgattaaaa aaagcagtct taaaaagggg gatggtaaat cattttcagc acttgcgaca
    13981 ctcgatttat cgggacttgg tggatatctg aaaatattgt ctgccagtgc agataatctg
    14041 gaaatttttg aaagtattta tcatgaagga atggagcctg atgactgggt tcctgaatat
    14101 ctcgaacggg caatctgaca ggtgaattta tgaaacgtgt aaaaacattg atgctgatat
    14161 cattattgac ggcttcattt tatagtagcg caggaattcc ggttgctatt gatgccagtc
    14221 cggaatgggc tgttgaagcc gcaagatgga cagaacgact caaacaatgg tccgaaactg
    14281 cgcaacatta tcagagtcag attcaggcat ataaagacca gttagccacg gcaacaggta
    14341 taaggaatat tgcggcattc acaaatgaac taagtaatct acaatcagaa ctgacaaata
    14401 tttataagca ggggaacagt tatatttctg attttaccag taatcccgaa ggtgcattga
    14461 gcagccaggc taaaaatctg ttcagcaagt atggagcctt cgatatgtgt aatacgggct
    14521 atgaacgtaa tgataatctc tgtaaagcac ggattgtgtc aacggctgcc agtattgaac
    14581 agggaaatga gatcaataag cagttatcaa gtgcaatgtc tcagattcag agtttaagta
    14641 gccgaattga ggcttcaaag gatatcaaag aatcccagga tttggctaac gcattacagg
    14701 cgcagtcgtt aaaaatgcag gcgataaaaa tgcagtatga tgtctggaat aataaaaata
    14761 aagcagatca tgagatgttg gtcacacaag aacaagaggc ctttattaaa cagcagaaag
    14821 aagctgaacc attaactttt gattaaaagg tggatatatg gctcaggggt ttttcgttaa
    14881 atataattct accgttatgg attcggtaga taaaatcagc tccagttatc aaacacaatt
    14941 tgctaatgat attatgtcgt tagcaacggt atcggttacg ttatatgtac tatggaaagg
    15001 atatcaaata cttgccagta aaactcagac gcccttgcag gatttagtat gggatctatc
    15061 aaaatttgca atcataatca tgtttataac taatgcggat gggtatttaa ctgcggctac
    15121 tgatgcgttg caaggcatga aagatggttt ttctggaggg gtgagtgtct ggcaaacttt
    15181 agataactta tggaaaagta cgcaaaatct tggtgcggaa atatactctt tagataaatc
    15241 aacatatgtt aaagatcaag gtgtagttgg tcagttttta atatggactg gctcgcttat
    15301 tttaatggct gttagtgtgg ttgttttttt aacagcagat gtaacgatga aactcctgat
    15361 tattacagca ccgatattta tcttttgtct catgtttggt tttattcgga gcatgtttaa
    15421 taattggtta caatcgttat tctccagtat tctgacagtt ctatttgcca gtttagtgat
    15481 tcggatagca atggattttc aagggatgat cctttcccat gctattcgtg ctgcacaaac
    15541 agggaatgta aatcttgtat ctacaggtgc tatgggcttt atggctgggt ttttgggggc
    15601 tttattggtt cttatagcta aaggttttgc tgtccagtta gcaggtgcag gtgttgaagg
    15661 tgctgttcag ggcgcagcca tgatggggct gggtgctgct ggtatggcta ctggtaagtc
    15721 tctgatgctt ggcggtcgtg ctggtttagg ttttggctta gggatggccg gaagaacggg
    15781 catgaattct ttatcaggga aagcagggaa tctgatgggg cgtggtgcgc gaacagccgc
    15841 tgaatgggcg ggtgaaaagg gatatcaggc gctggctccg acaggtgctt tggggatgaa
    15901 ggcgagaaga cttgcatccc tggaaaaagc acgagcgagg aacgccgcat gaaaataatg
    15961 aaacgttttt tttatgtgat gtccctgaac tggatactgg tgttcgttga taaacatatc
    16021 acctccggat ggagcaggaa gagaaagcta ataacccagc ttgttatttt aatcacggga
    16081 attaccttat ttaaggtctt aggtggaatc agtcagagct ggttgtgaca tgcttcatta
    16141 ttttgtcatg atactttatt ttgtcctaat ccttctttta ggagtgttta cctgcttcgc
    16201 aggaataaga cttggtttag ggcttgcggg gaaatggaga aagggtatgg atcttcgctt
    16261 tttgcttctt attggtggga ttctttttct tcatcttggc ctttatctct taaaggtgtt
    16321 cgtgaacgct taataaggtg aatttatgcg atgtgtgatt cttttgtttt tcctgtttca
    16381 gatttccgga tgtaccacaa ggaataacct tccttctgat gtttccggcg aactggaacc
    16441 cattaaccac agccaggtaa tcagttatga gtgaaaaaat cgaaaagaag attgagacag
    16501 ctaaatcctt cgaaagacaa ctctatgcag aaaatgaacg gtctaagaaa aatgcatggc
    16561 gtgttgcagt ggcagcatca ttactgtcct tatgtcttgg ggctgcaatt tgtgttcttg
    16621 tgccattaaa agaaaaagag cttgctattg tggctgttgg cgaaaaaaca ggccgtacag
    16681 aactgattac tcaggttaaa caggaaaaga tcctgcaatc tgaagcactt gggcggtatt
    16741 ttgtaaacac gtatattact ttacgtgaag gttataatta tccgtctctt cagtatgatt
    16801 atgagactgt acagctatat agctcgaata cagtaaagga cgattatctt cgtctgtata
    16861 acagcgatat ggctcctgat aaaatttatc ataataacgg ttcttacgtt gcggttgagg
    16921 tcatctccaa tattatttct gacgcaaccg cgccagataa actggcctct gtgcgcttta
    16981 aaaaaacgac gagaaacttc acgaccggac aggtttcagt ttcatactgg acggctcgtg
    17041 tgacatatcg ttttgaacca gaaaagtcgg ttaaaagttc cagtcgtgag cttaatcctc
    17101 tcggttttac tgtaaccagt taccagacag atcgggaagt caggggggaa tgatgaagaa
    17161 attatttatt gctacatgtt tattgcttcc tgggcttgtg tattcagcag cgactccgtt
    17221 accttctgga tttgatgcaa ggatgcagac tgtgagttat aacggtgcga atacgaccgt
    17281 tatccggtca aagacaggat ttctcacatc agttgttttt gatgaaggtg aggctgttat
    17341 cagtgcaaag gctggttttc ctgccggatg ggaaatcaca accgatgata atgttgttta
    17401 catcaatccg cgtcccgttg ttcaggaaca ggaaggcgat gaaggcgaaa aactgaaaaa
    17461 ggttttccag ccaacagaaa aggaatggga tacgaatctt tttgtcagaa caacaaagcg
    17521 gatttacagt ctggatctaa tccttctgtc agaagaaaaa caagcgcaac ctgcttatgt
    17581 tgtacaattc cgctatccat cagaaatagc gaaaaagaat gcagaagaag taaggctggc
    17641 aaaagaaaaa caggaaaaac tcagacagaa gaaactgatt tctgaaagtt ttgaaaaagc
    17701 cgatgcgcct aaaaactgga attatttcat gcgagtcaat gaaaaatatg acagtcgtcg
    17761 tatcgcccct gattttgctt atgataatgg catatttacc tttttaggtt ttaactcagg
    17821 gaaagtattc ccagctccat ttgctgtaag agatggtcag gaacagacac ttgcttttaa
    17881 tgtggaaaca aaaggcaagt ataaaattat ggttatccac aacgttaatg acaaatttgt
    17941 tttacgctat ggaaacagcg ttgttggtgt tgtgaataaa tctttcggaa aagtattaac
    18001 cgaccagagg aatacatctt ctcctgcggt agaacgtgtt gaggtgaata atgactgata
    18061 tgaaaaaaac agatgattta gttgatgatg cgaatcagtc aaagctggaa agtaaacccg
    18121 ttcatgatat cggtgatatc aggaacaaaa ataatcgtat gcgttctggc tttctgtttg
    18181 gtctgatggc tgtattagtt attggtattt ttgcgctgaa aacatataaa aattattttg
    18241 cagatgacca gcagaaagca tcagaatcaa caggcgatac cagcatttct caggtaagta
    18301 aaatccgtac tggtcttggt cagaattttg accctgttga gaacaaagca attatcaatc
    18361 caggcgctac tggttctggt aatactgttt ctgacggtca taaagaagtg ccacaggagg
    18421 ggttcaggaa atatttgtca atacctgttg ctggtcaggg agggagtcag actggcgcga
    18481 gtggcagcag caggacatcg cagacatcag agccacagga agaaaggaaa gaatcaaaag
    18541 aaaatgtgcc tggtaaatca ggaatgaaag taactgcgat taatctcgat cctgatctct
    18601 atattgagga aaacaggctt ataccgtgtg ctttaaccac acgctttgtt tctgatgttg
    18661 caggtcgtat cagttgtgtt tttacagaag atgtatggag tgctaatcac cacacaaaat
    18721 tactggaaca gggaactaag gcttttggtc gttaccagac cggaacgctg aatcatggac
    18781 agggcagaat gttcgttatg tgggagcagt tgcggacgcc tgataataaa cgaatcgata
    18841 tggtaaatac agcagcagct ggtcccctgg gagaagcggg cattgatgga tggatcgatt
    18901 ctcacttctg ggagcgtttt ggtggttctc ttatgctcag tatggttcag gatgtagcgg
    18961 cggcggcggc ggataatgca cctggtaaag atcgtaatgt ggattatacc gaaaactcac
    19021 gacaggcaat ggctgaaatg gctaaagtcg cacttgagaa ttcgataaat attcctccaa
    19081 caatgtataa aaaccagggt gacataatca ctattatggt cggggaagac attgattttt
    19141 ctgatatcta tgagttaaag gtaaaatagt atgagtggcg ttattcctgt cagcattagc
    19201 aataaatcgc tcgattttta taaaaatcaa gtattccagt cttttcttga tattgaaggg
    19261 ctaacagaaa ttgctgtcaa ccgtcctggt gaaatctgga cggaaattaa tggtgactgg
    19321 acattccata ataatgacag tgtgaccttt gatttttgta aacgtttttc tcatacgctg
    19381 gcatcatttc ggggagatga aatcggcgag accaagccat tactttcagc aacactggaa
    19441 tcaggagaac gtgtacaggt cgtttttcct cctgcatgtg agagaaatac tatttcaatc
    19501 actatacgta aaccatcgac acggcagata acacataaag aatatgtggc gaacggtttt
    19561 tatgattatg tccagtcagg aaaaaaacac aggacatacg atgatgagct tcttgattta
    19621 ttcagagcga gaaatatcgc tgcttttatg gagcttgccg tagcagcagg gaaaaccatt
    19681 gtatttgccg gagcaacggg ttccggtaaa acaacttata tgaagagtct tatcgatttc
    19741 ataccattga atacacggct gattacgatt gaagatgcag aggaaatcaa attttttatc
    19801 cataaaaatt atgtgcatct tttttaccct tcggaatccg gcagtgattc cggctctatc
    19861 ataacagcag cgaaattaat caaatcctgt ttgaggatga aacctgacag aattttactg
    19921 gcggaaatta aaggtggcga tgcctgggat tttgtgaaag ttgctggttc tggtcacagt
    19981 ggaagcatga cgtccattca cgcggcctcg gcaaaagatg ccatcattca gatggtgaca
    20041 aaatgttatc agaacagaga atgtcagaat cttccttttg atgttcttcg taaaatcatt
    20101 atggacagta ttgatattgt tgttcatgta ggcagggatg gtgttgtcag gcatatgagc
    20161 gacatctatt acaaaggggc tgaatgtgaa aacttttaat aagtcgcaat ctgtttttat
    20221 attcgctgtt atgcttgttg cggcatggtt tgcaggttca tttattttct ttgtgcttta
    20281 cttcagccag ataaagaatc tgaaaccatt gaaggctgtg ttcagatcca ttgatgcgta
    20341 caggatggat atttttacag acagccttac tttatctgtt gtgactacgg atatcagagt
    20401 tttagccttt gttgcacttg gagcaggatt tgtcgtgtct cttatcatcc ctgttgcggc
    20461 gttgataaaa ctgaacgaga agaaagaaaa tattttcggt gatgcgcgat ttgcaacaat
    20521 taatgatatt cgtgagtcta acagtttcac gcttgacggt gacgaaaaag acgggattat
    20581 cgttggtata aaagacaaga aaatcatacg ctatgtggga gcagcattca gtgctatggg
    20641 agccggaaca agggctggta aaggtgctgg tatagtcatc actaatctta tgaagtactg
    20701 gtggagtgtt attattctcg atccaaaaag ggagtgtttt aatatcacca gtcttatcag
    20761 gaaggtgatt ttaggtcatg aggtttataa atttgatccc tttagttcgg taacgcaccg
    20821 ctttaaccct ttgtattatg tcagcatggg aacgagtgaa ggttttaatc agcttgaaaa
    20881 tctttcgttg attatttacc cctataagac agatggtgcg gatgctggta gctatcttaa
    20941 taagacggcg ggtggtgttt ttaaatctta tgctgtcgct ttatggttta tgattaagaa
    21001 tgataaggct ggactaaaaa cgcttgatat tgagcctgtc ttctcaatgt ctaaaattaa
    21061 tcagcttttt gagcgtgcag aacccgaaca tcttctttca ttcatgaatg atattcgttc
    21121 agaattaagg gggaaagaca aaacgcttgc tgatattggt gtcgctggcc ttaaggcatt
    21181 cattgagatg gaggataaaa caaaagcaca actcaaaagc aactttctta atggattatc
    21241 accgtttgct aaccccaatg tggcaaatgc tactgatgga aatgattttg atttgcggca
    21301 ggtgcggaaa aagcggatga caatttattt ctgtatttct ggtgataatg ccaggttggc
    21361 tgaaaaaata acaaatattt tctttcagct tgctatacag gtgaatcttg aaaagatgcc
    21421 aacggatgat ccggaaataa agcatgactg cctttttcta ctggatgaat tcccgtcaat
    21481 tggtgctgta gattacatca agagtaaatc tggtctcatt gctggttaca agctgaaatt
    21541 gttgattgtt taccaggtcg gctctcagct tgaggaaatc tacagttatg caggcagtaa
    21601 aacattactg gcaagtgcac cctgcaaaat tgtttattcc gcttctgatg ttaaagatgc
    21661 gcgggagtta tcagaagcga tggggacacg aacagtaact atcggttcga aaagcaaaag
    21721 tcgtagtcgt ggaggaacat cgagatctga atcagaaagc cttattgagc gtccactggt
    21781 aacaacaaat gaactactta cactgaaatt tagtgaagaa atcctggcaa tgaagggaga
    21841 aaacccgatt cgttgtgaaa aggcattcta ctatctcaat gagtatttct ttggtgactt
    21901 tgtgaaggtt gctccggaac ttgcaaacat tttccccagg aagaaaggaa agctggttat
    21961 gcctccacag aaagtatttg aaaatgaaat cgtcgcaaaa gggtatctgg tagtaaaaga
    22021 tgttcctgat ctcgataaag cggcataaag gagaacatga tgaagaagtt agtgttttta
    22081 ttgatttcca ttcttgctgg ttgttcatca ccgccagagc caacacccgt gcagtttgaa
    22141 aaggcaaatg aagtgatcaa tccctcattg ccatatgtcc ccgattttca cggtgtcata
    22201 aagtcagacg ttagcggtaa aggatgggtt tatgagatca cttccttatc cggagtacag
    22261 gccagaacgc cgacgtttta ttacgctctg gctcatgcgg acaggattgt agtgacaaca
    22321 catgatgctg ggctgtggtt tcgaatcaga gatctgttga agatggaagg ggcaacagca
    22381 gtcgtggaat ggcgaaacga gaaaagtttt ttacctgaac aggtaaaaat agttttcatt
    22441 aaagcacaaa atgaggtgaa aaatgactgg aaaaaataga gtggttttac cagccttgct
    22501 ggcgttagga ttaatatcaa caaatgctaa tgcatctgac ccttgcgctt cggtactttg
    22561 cttatatgga aaagcagtcg ggcaaggtgg agggagtgaa tgcagaagtg ctgaaaagga
    22621 tttttttaat attctgaaaa agaaaaaagg aagcattcgc tggagtaaaa cttttgatgc
    22681 ccgtaaagca tttctgaatc agtgttcaac ggctgatcct gctgctatat caaaaatcat
    22741 gagtaagttt ggtcgtgtca gaggttaatt aaaacctgat aaccgcgcca acgggtatca
    22801 gggtactgca agtatcacat ttcgcgccaa cgaaatgtga tgattagtta caatcactga
    22861 ggttagaaat gaaagtaacc aaatctatta tagctatttt attgtgctta tctgctggta
    22921 gtgcataagt ggatgtaata aaaaatgctc gtgccttttt gaaaggaacc ctcgatctga
    22981 gcgttcagga agcagatgaa gatatagagg aaagggaaga actttataaa aagaacgggg
    23041 cacaacctga ctatttaagt tacctggagg attaacacat gcaatatgca ttgtttgatg
    23101 gatttgaacg aaagtttttg ctggatgctc ttgaattcgg tgttctgaag gactggaaag
    23161 aaaatccggt aaaagaactt cctgatattg atgaatctgc tcaccccttc catatctgtt
    23221 atggtggata tttattaaac cctggtgttt cagattcaga tattagcaga aaaataaaag
    23281 accaggcagg attctggctg gcagctattg atgatacccg catggattgt cattcaatag
    23341 cttattatga tattcacaca ctccctctga tttcgtgtgg tcatcaaaag atagttcctt
    23401 ttgccgcgtt aataaaagct gatgaatgca tcatttcaaa aattgcttcg tattctggtt
    23461 ttgctgtaac agcctttttg agaattaaag agtgggatat tgcaactaat atacttaacc
    23521 gtgagggaat ttttgccttt aatggctgtg agcgcagatt cagagtagta agtaaagata
    23581 actggcaaca tacagtatcg gaagaacgcg ctatccgttg tgccaaaaga ttaattcaat
    23641 gtaaaggata acaaaatgag actttttatc gcagaaaaac ccgcagtagc aaatgatatt
    23701 gttaaggcac ttggtggcaa ttttacccgc catgatggct ggtttgaaag tgataacgcc
    23761 attgtgacta actgttttgg tcatattatc gaatcacaac cgccggaaaa ctataatcct
    23821 gaatacaaag cctggaaggt tgaaacgctt cctttacgtc tttatcccgt gaagtatcag
    23881 cctgttgaaa gtgcagcaaa acaggttaaa acgattctcg aacttatcag acgtggagac
    23941 gtgactgaaa ttgttcacgc tggcgatcct gatgatgagg gacagctact ggttgatgaa
    24001 gtcctggaat atgcaggcaa cacaaaaccc gtaaagcgcg ttctgattaa cgacaacacg
    24061 cttccggcag tgaaaaaggc actggcaaat cttaaagaca atcgtgattt caaaggactt
    24121 taccttaagg cgttggcgcg gtcagttgcc gatgccgtct atggcttctc tatgacacgt
    24181 gcgtacacca ttccggcaaa agccagagga tatcagggcg ttctgtctgt cgggcgcgtc
    24241 cagactcccg ttcttggcct gattgtgaat cgtacccgtg ctaaccagaa ccataaatcc
    24301 agtttttact acaccatgac cggagtcttt cagcgtggtg ctgatgttat cagggcgaac
    24361 tggaagccag gggaatttgc tccgctgaca gaccgtaaat tacttgataa agcgtgggca
    24421 gacggaacgg ctgcatccct tgcaggaaaa ccggctacag ttgaagcagc agcaactgat
    24481 gataaaaaaa cggctgcgcc gttgccgttt aatctggtca gactccagca atacatgaac
    24541 aagaagttta aaatgacggc acaaaaaacg ctggatatta cgcaacaact acgcgaaaaa
    24601 tataaagcaa ttacgtataa ccgctctgac tgctcatatc tttcagatga acaattcagc
    24661 gaagcgccgc aggttatcga tgccctgaaa tcagtctttc ctcagtcgct ggatattgat
    24721 tctgcacgta aaagcaaagc gtttaacagt gcaaaggtga ctgcgcatac tgcgataatc
    24781 ccgaccgcca gtgtgcctga tgttaacgca ctcagcaccg acgagcgcaa tgtttacctg
    24841 gcgatcgcac aacactatct tgttcagttc atgcctgaaa aagcatacca ggaagtatcg
    24901 gttgccattc agtgtggtga tgagtcgttc tatgcccgtg ccagaaaaac aactgacagc
    24961 ggatttgagg cgtttcttgg cgcggaaacc acagacgaag gtgaatcaga agataatgat
    25021 gattccgctt ttgaactgct ctgtaaaatt cgcacaggag aaacactgac gacaaaagaa
    25081 gttattgtta atgagaagaa aacaacaccg ccgccgttat tcaccgaagc ctccttgctt
    25141 gctgcgcttg ttcgtgtcgc ggattttgtc actgatccaa caattaaaaa attgttgaag
    25201 gataaggata aagacaaaaa agatgaacat ggtggcattg gtacgccagc tacccgcgca
    25261 gccattctgg aaacgctgaa gaaaagaaac tatatcacgc tggaaaaagg gaaacttatt
    25321 cctactgata ccggatatgc gcttattgat gccctgcctg gtatagcggt taatcctgat
    25381 atgacagcat tatggtctga aaagcaggct gccatagaaa atggcgacct gacggttgaa
    25441 cagtttatta atgatctgta cggtgaactg acaggcatga tttctgatgt tgacctgggc
    25501 gagatgaaga ttgaacccgc tgcgccagca gggcagtttc aacgcctgga ctctccctgc
    25561 ccttcctgtg gtaaacatat tgttatcagg ccgaaaggtt atttctgtac cggatgtgaa
    25621 tttaaaatct ggagtgagtt ttctggtaag aaaatcaccc aggcacaggc cgaaaaactg
    25681 gttaaatcag ggaaaaccga tttgattaag ggatttaaaa agaaaagtgg tggaacgtat
    25741 gatacagttc ttgtccttga ggataagaaa acagggaagc tgggttttcc ggcaagggct
    25801 aagaagtgaa aacaaagcag gaatggcttt ttcagttaag aaaatgtaca tcaagagata
    25861 ctcttgaaaa agttattgag attaaccgtt acaaactgcc tttatcagaa tcagaggcat
    25921 tttattctgc cgcagatcat cgccgagcag aactggtgat gaataaactt tatgataagg
    25981 tgccttccgg cgtctggaag tacgtacact aaataagagg attaattata tgagcgaact
    26041 gactaaagaa gatgaatacg gcattatcag ccgtactatg atgaatattc gttcattgcg
    26101 tgtttttgcc cgtgagattg attttgagca gttgctcgaa atgcaggaaa agctcaacgt
    26161 tgttattgaa gaacgtcgtg aagatgctga acgtgaagcg gctgaacgcc aggaacttga
    26221 agccaaacgt cagcaggcca ttgaatacat catatcttta ggtcttgatc ccgaatcact
    26281 ccttgctcct gtaacagcag atacagtaaa aaccaggcgg aaagccaaag gcggagtaag
    26341 aaaggctaaa taccgcttta aggatgaaaa tggcgagatc agagaatggt ctggtaatgg
    26401 caagcgtccg cttgctttac agaagctgct tgatgagggg catttcatgg aagatttcct
    26461 cattgagaaa actaagccgg aacaggcaga ataacccgcc aagtatacat cacacagggc
    26521 actgttgcaa atagtcggtg gtgataaact tatcatcccc ttttgctgat ggagctgcac
    26581 atgaacccat tcaaaggccg gcattttcag cgtgacatca ttctgtgggc cgtacgctgg
    26641 tactgcaaat acggcatcag ttaccgtgag ctgcaggaga tgctggctga acgcggagtg
    26701 aatgtcgatc actccacgat ttaccgctgg gttcagcgtt atgcgcctga aatggaaaaa
    26761 cggctgcgct ggtactggcg taacccttcc gatctttgcc cgtggcacat ggatgaaacc
    26821 tacgtgaagg tcaatggccg ctgggcgtat ctgtaccggg ccgtcgacag ccggggccgc
    26881 actgtcgatt tttatctctc ctcccgtcgt aacagcaaag ctgcataccg gtttctgggt
    26941 aaaatcctca acaacgtgaa gaagtggcag atcccgcgat tcatcaacac ggataaagcg
    27001 cccgcctatg gtcgcgcgct tgctctgctc aaacgcgaag gccggtgccc gtctgacgtt
    27061 gaacaccgac agattaagta ccggaacaac gtgattgaat gcgatcatgg caaactgaaa
    27121 cggataatcg gcgccacgct gggatttaaa tccatgaaga cggcttacgc caccatcaaa
    27181 ggtattgagg tgatgcgtgc actacgcaaa ggccaggcct cagcatttta ttatggtgat
    27241 cccctgggcg aaatgcgcct ggtaagcaga gtttttgaaa tgtaaggcct ttgaataaga
    27301 caaaaggctg cctcatcgct aactttgcaa cagtgcctca cacagacata cacataggta
    27361 tgtctgtgtg acatacctat gtacagtgcc agatggcgtt gctacgtgaa aaataaccta
    27421 ccctacccct gcttttttcg cagattttac gtagcacacc gttgctatag cattcatcta
    27481 aaaagctgaa tgtccttttc tgtcgctgtt gcattttaat tcactgaaaa atattgagta
    27541 atgaaaatgc cggtaacgcg tcaagctgtt ggccaacgcg acaggcagta ttggaagcaa
    27601 caaaaaaaat gaaagaaaaa tccctcaaat gaaggagaaa aaacggaact acagccgaac
    27661 gacaggcaca aaaaaacccg actggtaatc gggctttttt gcgttttcca gtcttgagtt
    27721 ggtggctctg accggagaag gttactctac acctaacgtt tacattttat tcactatggt
    27781 gctgtaaagc aactaaatgt ggttaaggaa ccacatttag tggtgtaacg ggtgttttgt
    27841 gtagcgtata atcagtcagt tagtcatgta gttggcttgc aatcctgctg ttcccgtatc
    27901 tgcgggtatg aacgatttta aagtcctggc tggataaaca ggtggtgctg tattcggtgc
    27961 tctggcagga ggaggtcact ctacacctaa caatgcgaga gagccgttgt gacaggcagt
    28021 gtatgagcaa gcaacggtga tttcctccag ttttactgga gaaacggatg attaatatgt
    28081 taatcatcgt tctcagagct gtggtcgccg ttgcaaacgc gctgattgca gttctggaac
    28141 tgatccgcca attcatcgat tgatgacaac ggaaacgtaa ctaagggcag gaagtgaacc
    28201 gcttcctgcc ctttaacaaa aaaggaggat ttatggattt tatgagcatt ttggggcgct
    28261 gtttacttct tcctttccct gttgcgatgt ttgtcggttg cctgtttcct ggcatcgatg
    28321 atcgcttatc cggaatgctt atgagtgttg ctgtgggatg tttatcgtgg tatgtcacca
    28381 gaccgaagaa ccgacagaca aaggccgcgt aagtggcctt taattgggat accctttagt
    28441 tacaacattc taatagctca ttccatcctc cgctcatcca gcaaacataa cctccctgcc
    28501 cctccccact acagaaagtg gcgcgccccg cccgtacggg cggaatctaa tcgcttaatt
    28561 ttgaatgttg taactaaaca tactcattgc tgtattgcca caaggtttcg cagcaaggga
    28621 ggataccgaa agtaacgctg tcacttttgc tttcaagtga ccaaccaacg ggtacatcac
    28681 actgacatac aataatgtat ttcttcatga tgtacatcat tgtaattcat tgtgacatac
    28741 actatagtat gtccatgtga catacgcatg cgtacatcac atagaaatac acatgagtaa
    28801 atcactgtga catacaaggg gtactatgat tatcgtaatc ggtggtgata aaggcggtac
    28861 aggtaaatca catctggcaa ccaacttaac tgtttgcctc tcacaacagg gaaagagaac
    28921 ggggcttgtt gaaacagacc tgaatggctc gaccaaaaaa tggaataaac gcagagaaca
    28981 ggccggatta ccgcccgtcg cattaaatga agcctatggc gacatcagtg ccaaaataac
    29041 aaaaatggca gatgtagcag aaatcctcat tatagatact gccggatatg acagcacgga
    29101 gttcagaact gcactaaaag ttgcagacat tgtgattgtc ccgatcgacc cgcttgccca
    29161 ggtggaagca gacagtcttc agactgtgac aaaaattgtc agggatgcgc aaaaaataaa
    29221 tcccaggctg gctgcacatg tgctcctgta taaatgccag ccaaatacgt tcagtgaaca
    29281 acaagaatta agagactctc tcaacagcca tgattactgg cttaaaccaa tgaaaagcac
    29341 ggtttctttt ctccgggcct ttgtaagagc aatgaatcag ggaatggggg ttcacgagtt
    29401 aaaaagtaat gttagcggtg ccagccaggc aaaagcacaa atcgaactct tgctgaaaga
    29461 actggaactg taaatcatag tgacatacac taatgtacat catcatgata tacattagtg
    29521 tgaaacaatc agattagaag gtacatcatg gcaaagctgg aactaccaga actagaacaa
    29581 aaccaggaag tgaacgccac aaaatttgtt caggacgcag gaaaacgccc cacagagaac
    29641 aaaacaaaac tggcttcatt ccgcataccg aatgaattgc ttgaattcat tgataacgaa
    29701 tcaaaaagac tgcatcgtcc caagacaaaa ataatcaaag cagcattact ggcttatcag
    29761 gatctggacg aaaacgaact cattaatttg tggttcagag ctgacagaaa tgattaatca
    29821 tagcaagtgg cccaataaac tctttatcat tttcttttag atacttatct gaatgtacta
    29881 acttcatcac ttttaaatgt agttgtgatt catttttagt tactcttgca ataaaatacc
    29941 cttgtacagt tgttgcacgg gtatttagga gcactagatg agccttatcg atttaagttt
    30001 atcaggatta tcagaaccag gaacaaaact tattgaaaaa ataagtgatg ctattggtgt
    30061 gctttatgaa cctacaagaa tcaggaaaaa agcgaaggct gaagctgaag ctaaacgcac
    30121 agagctaatt tctaggttag agcttgaggg aatagaaaaa cgggcagttg agcgttttct
    30181 caaacgtgaa acgaaacgac aggaaaacat tgaaaatata acaatgcaag cagcacaaag
    30241 tctatctgag agtgataatg tttctgatat tgacgaagat tggatagagg ctttcttcag
    30301 ggagtgtgag gatattagtg acgaacaaat gcaaatgctt tggggaagaa ttttatccga
    30361 agaagcaaag tcaaaaggtt cgtttagtcg tagaacctta aaactattat ccactataag
    30421 caaagaagaa gcgaacctta ttacttattt tggaaagttt gtttggcagg caaacaaact
    30481 cactccaatt ttatttaccg atgagaatgg agatactgaa ggtataactt tcgataaact
    30541 ttcggtttta gactccttag gcgttattca acaaggcatt ggttatagtt taagatacaa
    30601 attataaata ctctcaagtg tatattcagt atgggattgc gcaatgattg cctaataaaa
    30661 tttctgaaat atttctgtat cgcataattt tttatatcag ataaactgta ctggatttct
    30721 taaaaaattg cagtataatt gccgcaatta tcccaccgtt tattttttga gtagtttctc
    30781 atgatgcagc atacttctgt gtggtaccga cgctcggtca gtccgtttgt tcttgtggcg
    30841 agtgttgccg ttttcttgac cgcgaccgcc aatcttacct tttttgataa aatcagccaa
    30901 acctatccca tcgcggacaa tctcggcttt gtgctgacga tcgctgtcgt gctctttggc
    30961 gcgatgctac tgatcaccac gctgttatca tcgtatcgct atgtgctaaa gcctgtgttg
    31021 attttgctat taatcatggg cgcggtgacc agttatttta ctgacactta tggcacggtc
    31081 tatgatacga ccatgctcca aaatgcccta cagaccgacc aagccgagac caaggatcta
    31141 ttaaacgcag cgtttatcat gcgtatcatt ggtttgggtg tgctaccaag tttgcttgtg
    31201 gcttttgtta aggtggatta tccgacttgg ggcaagggtt tgatgcgccg attgggcttg
    31261 atcgtggcaa gtcttgcgct gattttactg cctgtggtgg cgttcagcag tcattatgcc
    31321 agtttctttc gcgtgcataa gccgctgcgt agctatgtca atccgatcat gccaatctac
    31381 tcggtgggta agcttgccag tattgagtat aaaaaagcca gtgcgccaaa agataccatt
    31441 tatcacgcca aagacgcggt acaagcaacc aagcctgata tgcgtaagcc acgcctagtg
    31501 gtgttcgtcg tcggtgagac ggcacgcgcc gatcatgtca gcttcaatgg ctatgagcgc
    31561 gatactttcc cacagcttgc caagatcgat ggcgtgacca attttagcaa tgtcacatcg
    31621 tgcggcacat cgacggcgta ttctgtgccg tgtatgttca gctatctggg cgcggatgag
    31681 tatgatgtcg ataccgccaa ataccaagaa aatgtgctgg atacgctgga tcgcttgggc
    31741 gtaagtatct tgtggcgtga taataattcg gactcaaaag gcgtgatgga taagctgcca
    31801 aaagcgcaat ttgccgatta taaatccgcg accaacaacg ccatctgcaa caccaatcct
    31861 tataacgaat gccgcgatgt cggtatgctc gttggcttag atgactttgt cgctgccaat
    31921 aacggcaaag atatgctgat catgctgcac caaatgggca atcacgggcc tgcgtatttt
    31981 aagcgatatg atgaaaagtt tgccaaattc acgccagtgt gtgaaggtaa tgagcttgcc
    32041 aagtgcgaac atcagtcctt gatcaatgct tatgacaatg ccttgcttgc caccgatgat
    32101 ttcatcgctc aaagtatcca gtggctgcag acgcacagca atgcctatga tgtctcaatg
    32161 ctgtatgtca gcgatcatgg cgaaagtctg ggtgagaacg gtgtctatct acatggtatg
    32221 ccaaatgcct ttgcaccaaa agaacagcgc agtgtgcctg catttttctg gacggataag
    32281 caaactggca tcacgccaat ggcaaccgat accgtcctga cccatgacgc gatcacgccg
    32341 acattattaa agctgtttga tgtcaccgcg gacaaagtca aagaccgcac cgcattcatc
    32401 cgctgatttc tccctgtatt ttttccaaac ccaccgcaca ctccattcgt attatgggcg
    32461 gtggggtggg gtttgttatg ccgtatttat caaataaacg cctacttgct gagatgagta
    32521 tcgctcttgt catggcgatc gttgccacgc tgacccttga gcacagtcag attgatctga
    32581 tggtcgctga ttggttttat ctgggtatgg ggcattggat ggttgccaag caagcttttt
    32641 tgccagattt gctactgtat tctggactaa aaaagctgct gatggcgatg ctgatctact
    32701 tgctggttgc gaccatttgc cgtgcttatc atgagaaaaa gggcaatgct atcactgcca
    32761 agtggcttgt cccagtgaca aaatttcgcg tgcgtgagct tgcgtatctg gtgctgactt
    32821 tgatcctagt gccgacagtt gtcgcgtcat tgaaggcata tactcatgtg gtctgccctg
    32881 tgcatttgac gatttttgat ggtacgctgc cgtatttgcc gatgcttgat agtatgcgta
    32941 acaccattcc tgataagtgc tttcctgcgg cgcatgccag tagcggattt gcgctgtttg
    33001 cctttgcgtt tgcgccaagt ttgcgccgcc gtcgtggtgc gatcatcatc gtggtgatgg
    33061 cattgggctg ggcgatgggc tgctataaga tgattattgg cgatcatttt ttgagccata
    33121 cggtggtgtc gatgatgctt gcgtgggcga tgtcggcagg gcttgcgtgg gtgtttttta
    33181 agaagggtga acaagtttaa tcggctttaa aagtaaaaac ggtcgtatcg gttattacgg
    33241 aacggttttt tcagttgagt ttcaaagcga tgattcatca acctggaaac taccaatagg
    33301 aacggcttt
//
