LOCUS       NZ_CP048805             3511 bp    DNA     circular CON 22-DEC-2023
DEFINITION  Klebsiella pneumoniae strain EGKP4 plasmid pEGKP4.5, complete
            sequence.
ACCESSION   NZ_CP048805
VERSION     NZ_CP048805.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14082702
            Assembly: GCF_017161505.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Hamza,E., Dona,V., Hamza,D., Elhariri,M., Elhelw,R. and Perreten,V.
  TITLE     Klebsiella pneumoniae -Carbapenamese-producing, complete sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Hamza,E., Dona,V. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2020) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina
            NovaSeq6000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/22/2023 17:28:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,748
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,484
            CDSs (with protein)               :: 5,484
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 56 of 140
            Pseudo Genes (incomplete)         :: 80 of 140
            Pseudo Genes (internal stop)      :: 27 of 140
            Pseudo Genes (multiple problems)  :: 20 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048805.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="EGKP4"
                     /isolation_source="fecal isolate from poultry farm worker"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pEGKP4.5"
                     /geo_loc_name="Egypt: Giza"
                     /collection_date="2015"
     gene            514..1011
                     /locus_tag="G4I41_RS28675"
                     /old_locus_tag="G4I41_29160"
     CDS             514..1011
                     /locus_tag="G4I41_RS28675"
                     /old_locus_tag="G4I41_29160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            1018..1233
                     /locus_tag="G4I41_RS28680"
                     /old_locus_tag="G4I41_29165"
     CDS             1018..1233
                     /locus_tag="G4I41_RS28680"
                     /old_locus_tag="G4I41_29165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1628..1810
                     /locus_tag="G4I41_RS28685"
                     /old_locus_tag="G4I41_29170"
     CDS             1628..1810
                     /locus_tag="G4I41_RS28685"
                     /old_locus_tag="G4I41_29170"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016265984.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     gene            3006..3347
                     /locus_tag="G4I41_RS28690"
                     /old_locus_tag="G4I41_29180"
     CDS             3006..3347
                     /locus_tag="G4I41_RS28690"
                     /old_locus_tag="G4I41_29180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          2748..2921
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(2260..2781)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.69"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4..1349
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3028..3341
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="9.860000000000001e-59"
     Typing          3338..3510
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.276"
                     /coverage="89.0"
                     /evalue="4.16e-28"
ORIGIN
        1 cattcgcgga ggcggacctg ccgccgggtc agcgtgaaaa actgatggcc agctttgagc
       61 gggttctgat gcccggactc gataaagacc agtacagcat tctgtgggtg gaacacgcag
      121 acaagggtcg tcttgagctg aattttctga tccccaatac cgaattactg acggggaaac
      181 gcctccagcc gtattacgat cgtgccgacc ggccgcgcat cgatgcgtgg cagacagtgg
      241 tcaacggcag gctggggcta catgatccga acgacccgga gaaccggcgg gcgctggtca
      301 cgccttcagg actgccagaa acgaagcagg aagccgcaga gacgattaca cggggtttgc
      361 tgatgttagc ctcatcagga gagctgaaaa cgcgtgatga cgtcacaggg gcgctcacag
      421 cggccggttt tgaggtggtc agaaccacaa aaaacagtat cagcattgcc gacccggacg
      481 gggggcgaaa tatccgactg aggggagcca tttatgagca gtcttttaac ggcctcgagg
      541 gacttcgagg agaaatcgaa agcacagcag cagcttaccg aagagaggct gaaagccgca
      601 ttcagcaagc acgagaacgt tgtcaaatcg gaactgaacg caagcgcgaa gagaatcaac
      661 gacgccatca gcgcccacga acaggggatg aacgcggcga tgcagtcgaa ccggctgagc
      721 gtgctgcgaa tggtcgggcg gacgtggctg accatcacca tggtgtcagg gctgctgttc
      781 gccagcctga gcggggtgct gtggtaccag gggaaccgga tagcggcgaa cctggcggaa
      841 atcaggcagc agagagatac gctgtcgaaa ctgcacatgc agacgtgggg cgtgacctat
      901 ctggagaacc gcaacggacg gtttctggtg ctgccggagg ggatgaaagc cgaaacgggc
      961 cggacggtgg acaacaagac gcggaacgcg gtgaagctgg tacgggagta gagcagaatg
     1021 acggaactgg aaaaacaatt gctgagcgcg ttagagcagc tacagcagga ctactcgaga
     1081 aggctggacg agtgggagag cgccttcgag gaatggcgga agatgtctgg tcttatgcaa
     1141 cgggagaacg cggcgctgag cgagcgcgtg tcgggcttga gcaggcaggt gcagagtttg
     1201 agcgagcagc tgcgccggtt atcgacaggc tgaatgagat tgagcaacag cgatacgggc
     1261 aaacaacgga aaaggaacca gagaagacag cagatctggt accggaacgg caacgcacat
     1321 tatctcatgg tgacagggag tatggagact gataaacgcc cgtctcaggt ggcctgagcg
     1381 tgacggggat catggccatt ttctgcgatt tcagcacaca gagagtcttt tttaaccgaa
     1441 atcaccggct atgcagtgat gtgaataagt gatgcatatg cgtaaaataa atcaggtgcg
     1501 tttctggtgg ttttcggtgt acttgccacc accttttgcc cgtcagccat cagaaacgcc
     1561 ctgagtgcct ccggcgcggt gcgggtaaga gatgttatgt ttaatgttga taacaggagt
     1621 aagagtgatg cttacattta ttggtgtagt ggtagtcgcc gttattgcat ttaaattatt
     1681 tatgcttttt tttagttcat ccgaatctgc cgtcgaacgt gctacccgta aatatatgga
     1741 gaaccctacg agtgctaact ataaaataat ggtggctaca cgcatgagaa atgagaagaa
     1801 aagagggtga tatatgtttg agtttattgg ttttttattt ttacttttgg tgtgttatgt
     1861 ctttatacaa atggttgctg tcagggtgtt tcctgaatat ggagtacgga aagcggagga
     1921 acgataccgt cgaaatccat cagcagaaaa tgaggatctg ttatggagag ctagaagtcg
     1981 tataactaaa cgcaagtaat gtttcctaag tgtgttgata ggcaaacatg gtgggagtta
     2041 ctactctcgg agttaatgat cttcttgtga tcctgtcgtt tcagtcgtaa tctcttgctc
     2101 tgaaaacgaa aaaaccacct ggggaggtgg tttgatcgaa ggttaagtca gttggggaac
     2161 tgcttaacca ggtaactggg tatacgagac cgcagatacc aaaactgttc tttcagtgta
     2221 gccgcagtca ggcccatact tcaagacctc tgaatctctc tctcgtatat cccagttacc
     2281 agtggctgct gccagtggcg ctttgtcgtg ctttctcggg ttggactcaa gatgatagtt
     2341 accgggtaag gcgcagcagt cggactgaac ggggggtttg tgcatacagt ccagcttgga
     2401 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agataaacgc ggccataaca
     2461 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag
     2521 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc
     2581 agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcttc gccgcggccc
     2641 tatcgcttcc ctgttaagta tcttcctggc atcttccagg aaatctccgc cccgttcgta
     2701 agccatttcc gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc
     2761 ggaatatatc ctgtatcaca tattctgctg acgcgccggt gccgcctttt ttctcctgcc
     2821 acatgaagca cttcactgac atcctcatcc gtgccaacat agtaagccag tatacactcc
     2881 gctagcgcta cgtgactggt tcagggctgc gccccgaaac cccactgacg cgcctgcggc
     2941 ttgtccaacc ccgcgccgac cgggaaaggc tttcccgtcc gtcacggcgt tatcaggagg
     3001 cgattctggc agataaacgc aacaagatgc tcacgatgtg ggtgacggaa gacgaacacc
     3061 ggcggttgct tgagcggtgc gacggaaaac aactggcggc gtggatgcgc cagacctgcc
     3121 ttgatgagaa accgacacgc gccggcaggc ttccctcgat ttcgcccgcg ctgctgcgtc
     3181 agcttgccgg catgggcaat aacctgaacc agatcgcccg gaaagtgaac gccggtggtg
     3241 ccggtcatga ccgggtgcag atcgtggcag cgctgatggc gatcgatgcc ggtctggaac
     3301 ggttgcggca tgccgtactg gaaaaagggc cggacgatga ttgttaaatt ccatccccgg
     3361 ggtcgcggcg ggggcgccgg tcctgtggat tatcttctgg gtaaagaccg gcagcgtgaa
     3421 ggtgccagcg tcctgcaggg gaagccggaa gaagtccggg agctgattga tgcgtcgccg
     3481 tatgtgaaaa aatatacctc cggggtgctg t
//
