LOCUS       NZ_CP048804             9294 bp    DNA     circular CON 22-DEC-2023
DEFINITION  Klebsiella pneumoniae strain EGKP4 plasmid pEGKP4.4, complete
            sequence.
ACCESSION   NZ_CP048804
VERSION     NZ_CP048804.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14082702
            Assembly: GCF_017161505.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Hamza,E., Dona,V., Hamza,D., Elhariri,M., Elhelw,R. and Perreten,V.
  TITLE     Klebsiella pneumoniae -Carbapenamese-producing, complete sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Hamza,E., Dona,V. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2020) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina
            NovaSeq6000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/22/2023 17:28:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,748
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,484
            CDSs (with protein)               :: 5,484
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 56 of 140
            Pseudo Genes (incomplete)         :: 80 of 140
            Pseudo Genes (internal stop)      :: 27 of 140
            Pseudo Genes (multiple problems)  :: 20 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048804.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="EGKP4"
                     /isolation_source="fecal isolate from poultry farm worker"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pEGKP4.4"
                     /geo_loc_name="Egypt: Giza"
                     /collection_date="2015"
     gene            join(9055..9294,1..1725)
                     /locus_tag="G4I41_RS28620"
                     /old_locus_tag="G4I41_29100"
     CDS             join(9055..9294,1..1725)
                     /locus_tag="G4I41_RS28620"
                     /old_locus_tag="G4I41_29100"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            1725..2456
                     /locus_tag="G4I41_RS28625"
                     /old_locus_tag="G4I41_29105"
     CDS             1725..2456
                     /locus_tag="G4I41_RS28625"
                     /old_locus_tag="G4I41_29105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            2463..2993
                     /locus_tag="G4I41_RS28630"
                     /old_locus_tag="G4I41_29110"
     CDS             2463..2993
                     /locus_tag="G4I41_RS28630"
                     /old_locus_tag="G4I41_29110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3021..3200)
                     /locus_tag="G4I41_RS28635"
                     /old_locus_tag="G4I41_29115"
     CDS             complement(3021..3200)
                     /locus_tag="G4I41_RS28635"
                     /old_locus_tag="G4I41_29115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3269..3646
                     /locus_tag="G4I41_RS28640"
                     /old_locus_tag="G4I41_29120"
     CDS             3269..3646
                     /locus_tag="G4I41_RS28640"
                     /old_locus_tag="G4I41_29120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(3708..4007)
                     /locus_tag="G4I41_RS28645"
                     /old_locus_tag="G4I41_29125"
     CDS             complement(3708..4007)
                     /locus_tag="G4I41_RS28645"
                     /old_locus_tag="G4I41_29125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3997..4260)
                     /locus_tag="G4I41_RS28650"
                     /old_locus_tag="G4I41_29130"
     CDS             complement(3997..4260)
                     /locus_tag="G4I41_RS28650"
                     /old_locus_tag="G4I41_29130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(4566..4985)
                     /locus_tag="G4I41_RS28655"
                     /old_locus_tag="G4I41_29135"
     CDS             complement(4566..4985)
                     /locus_tag="G4I41_RS28655"
                     /old_locus_tag="G4I41_29135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            5388..7073
                     /locus_tag="G4I41_RS28660"
                     /old_locus_tag="G4I41_29140"
     CDS             5388..7073
                     /locus_tag="G4I41_RS28660"
                     /old_locus_tag="G4I41_29140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            7083..7340
                     /locus_tag="G4I41_RS28665"
                     /old_locus_tag="G4I41_29145"
     CDS             7083..7340
                     /locus_tag="G4I41_RS28665"
                     /old_locus_tag="G4I41_29145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            7425..7574
                     /locus_tag="G4I41_RS28670"
                     /old_locus_tag="G4I41_29150"
     CDS             7425..7574
                     /locus_tag="G4I41_RS28670"
                     /old_locus_tag="G4I41_29150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             388..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(7678..8256)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1726..2453
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 ccattttcgg tgctggcctc gctggattcg cccgggcgga tggtcagggc acgtcgacat
       61 gtctggaact acgcgtactg gctgaccggc gcggccggtc tggcccttct gggggggctg
      121 gttgtcgccg tgtttatcga gagctgggga gaaaccgtcc tgcgtccgga cctctacgca
      181 aacgcagatt attacctgct gcagccggct aacctgttcg ggattgcctg gtgcgcgggc
      241 gggctggctg tcgggattgt gttggcgcgg ctgctggcgt tcctgctgct tgacggtcat
      301 tttgcggctg ctgacgaggc ggtgaacgcg aaactgaatc aggagagccg gcgcagcagc
      361 cagcggacag gagagatgac cgacgtgcgc cacctgcatt tcggggagcc ggtaccggtt
      421 aatgcgctgg ccgatttttc ctcggagcag gcccggcagg agcaggcggt gtttctgggt
      481 aaggatgagc agggcaagcc ggtgctggtt ccgcgtgata cctggcgcaa gaccaacatt
      541 cagatcctcg gcctgcccgg gagcggcaag agcgtgatgg ccaccaacgc gctcattcgc
      601 tgcgtacgcg atttcgggga cgcggtggtg tacttcgacc ccaaagggga cgcatgggcg
      661 ccgcacgtct tccgggcgca ctgtccggac ttcaccctgc tggatctgcg gccggggaaa
      721 ccggcgcagc tgaatctgtt ccgcgatctc gaccagtacg cgctgaaaaa cctgctgatt
      781 gccgggctta acctgggcga gaccggcgac gtggcggacc actaccgcat cagcgagcag
      841 aaagcggcga agctgattgc ggagcagttt ccgcaggggg caaacattca gcaggtgctg
      901 gcggcggcgt acgcgctgcc ggaggccctg aaaaaggacg tgaaggggtt gatcactagg
      961 ctggaaaacg tcgctgacct gagcgtcctg cagacggaca gtggcatcga tgtggcgggg
     1021 attatcaacg gcggcggctg cctgtacgtt atcggctcga tggacgacga ggcggtgatc
     1081 cgtgtgcaaa aaatgctgtt tgcccgctgc gcgcaaatca tcattgcgcg ggacgagttc
     1141 cgggagtggc cacacgtcag cgtgatgctc gatgaaatta aatatctgct gtcgaagtat
     1201 gtactgaacg cactggggac gctgcgcagc cgggactgca atctgttgct cgcgcaccag
     1261 tcgctgggcg atttcgggca gtgcgggcag gatctgcccg ctgaatttgt gaaaaccacg
     1321 gtactggata acacgccgat ccgctggttt taccgggcgg ccagcctgga gtccgcgcag
     1381 tgggcggcgg ggcagaccgg ggaaatcagg gtggacgtgg agcgccgccg tgccagccgg
     1441 gaggcgggga acgtggagca tatcagcggg gacagcttca tccagaagga ggcgcggccg
     1501 ctgtttgacg tgaacaccct gcaacacctg ccggacgggt tcgcggtcat gaccgggctg
     1561 ggtgtggccc ggatggcgtt cagcagtccg ctgcgggttg agcgccggga aatcccgttg
     1621 cagcgtttcc cggtgctggc gaaaaccgat cccctggcgg agttccggcc cccggccgac
     1681 ggcggcgggc cgaaagcgga tgatgatttt gcggggattt actgatggcg ctggagctgt
     1741 acaacgtcag ccacgcgaaa cgacaggcgc ggaacgcgga gaaaacccgg ctgaccctgc
     1801 gctggttgcg ggaagagctg tgttccacgg cggagctggt tgcgcggcgg ctggggattg
     1861 cggcggtgca gccggtctac cgcttcctcg acagcctggt ggcgaaaggg ctgctggtcc
     1921 ggaccaaata tccggtggac ggccgtcagg tcagcgtgtg ggggctgacg ccgcatggcg
     1981 tggcgttctc gttcgacgag gacgaaccgc tggcggatgt tattccgttt cagccgtcaa
     2041 gggtgtcggc agcacatctg ccgcaccggc tggccgtgca atccctgcgc cttgatatgg
     2101 aagccagagg ggccaccggc tggcgcattc tgcaccgtct gagtcttaaa ggcatgaaag
     2161 tgccggacgc cctggcggag ctggacggta aaacggtggc gtttgaggtg gagcgcaccg
     2221 tcaagagccg tcgccggtat caggaagtgg tgtccgggta tctgttcaac cgcaaagcta
     2281 acggcatcga tgaaatctgg tacatctgcc cggaccgggc gacgcaggtc agggtgcagc
     2341 gggcaatcct gtcggtggat gaaatcgtga acccacagac cggcgaggcc cgcaaaacgg
     2401 cagagctgga tcgtgaacgg ttgtttgcgt gctttaaatt tatgacaacg gagtgaggga
     2461 ttatggcgct gtacatggta tttattgaag acgcggagga ccggaacggc gagcgctgca
     2521 tcatgccgac cagcgagcat ctggaagata tggtggtgct cgcctggcag accggggcgt
     2581 atgttttctg ttttgtcgac cgggcccagc atctggcgat gatgaaatcc atcgggatgt
     2641 atgacgagcg gctcaacagc gacgaaaaag ccacgtactt tttcactggt aaaaatccgc
     2701 aatgggaacg gaacaaaccc acaggaatga agctgtgtga ttacctctat cgcaaggtgg
     2761 agcggttcgc catcgagaac ggtgtgcagg tggttgatgc cgggaagtgc tcaccggagc
     2821 agattaacgc gatgattgac gtggtgagtg agcggcacgg ggaaagcgcg gtgaacgaga
     2881 agctgaaagc ggagctgatg cgggaatata acagccgcta ttttaacgtg ccggtacctg
     2941 aactgccgga ggacaaacct gcccgccggt ggtggccgtt cggcaaaaaa taaatgccag
     3001 caccggccat gccggtgctg ttattcctcc ccgagcttat cccgcatcac ctgccagagt
     3061 gtttccgcat cctcgtgtag ccgctcgcac atgtcggccg gggtatccat atccagctca
     3121 ttcagtttat cgagcaatag cagcgactgc tccttaataa atttagccat attgagggct
     3181 gttttttcct gttttgtcat gattctttcc ttccgggcat ggtgtgcgcc tgtcaggtat
     3241 actgtctcaa aaatagcggg gtgagacaat ggcgtgggtg acggtgagac aggcgacgga
     3301 actgacgggt aaagcccgaa gttccctgta tcgggatatg gcgaaaggcc gcgtatcata
     3361 ccgtacggaa gctgatggcg gccgggtggt ggataccagc gagcttatcc gggtgtacgg
     3421 tgagttaaga caggatgaga ctcacgagcg ggacgagttg agactgtcgg gtgagactga
     3481 aaaaatggcc tccgacgcgt tgatcgcgga gataaaagca ctcagggagg aggtggccgg
     3541 gctacggcag gaaatgcagt ccatgagact gctggaacac aaaccggaga cagcagagac
     3601 cccggccgga acaccgcgcc ggtggtggcc gtggggaaaa cgctgacgcg ctgtgcttgt
     3661 ccaaccggcg acggccacgc aatgaattgc gtccccgtca ccggttatta gagaacaata
     3721 tggggattct ggcgctggcc gaccacggcc agaacccgga cttttctggt ctggtcatca
     3781 acatcataga ggacatgctg tcccatcaga ctgatccggc gaacgtcctc gccatagcgt
     3841 gagtccactt catagagtcg cgggaagtcg gccagtttgt ccgccgcagc gagaacccgg
     3901 tcggtaacgt tctgcgcatc ccacagggat gaattttccg cgatatagtc aaacagcgtc
     3961 gttacatcct cgatggctcc ctgcttccat tccacgtcat acggcatcgc gcagttcctg
     4021 ataacggcca tgcaggcgct ggcggagttg ttccgtggtc atacccggac gcgtgtcagc
     4081 acgggcggcg gtcactttgg cgcgaacctg atcttcatac tctgcctggg ccagttcttc
     4141 gttagccagt cgctgcaaca gcgcagcttt gtccgggcca ccttccggca gtatgcgggg
     4201 caggctttca gccagttccc gaagaaagtt ggcgttttcg tagtgtgtag tgttgctcat
     4261 tgcggagcct cccgggttaa tgcggcaata tgaaggaagt ttagcagggg gcggtctgaa
     4321 agaatatggt cgtgtcagtc aggctgatga agcagaaagt cggtaaaaaa gggcttacgg
     4381 catccatttt acgtcaaaac atatgccatg cagttgcgtg cataggcatg cattaggata
     4441 aaatttatcg ggcgcgtttc cggcagtttt ccgggggggt tgttgcctgt tttatcccgt
     4501 agccgccgga aacgccctga gcccgtctga gcggtgcgcg taatgacgcg ttatggtaaa
     4561 tccacttact gtctgccttc atagccatcg agataaacag ctgaaatggt gtcagccagt
     4621 agccttgatt ttttcggtgt gaggtctttg ctgcggtaat cggtacccgc aaattgtgtt
     4681 gtgccttgga gtgagtttaa aaattcctga ctgtgaaact gctgaatgcg ttgctgcaca
     4741 tcactccgtg attttccggt cgctttatcc tttgcgccca tatcccagaa ctgccggaaa
     4801 aaaggcacca tcattcgtgt tgtgtcagct ttctgggtgg aaaaattggg atcaaaaccg
     4861 tcatctgaag catatacgta atgccgggta tgctgttcca cgctgccctt tggagcacag
     4921 gcagtgagag ttgctgtcgc tgttatcatg gcgataagac acagtttgct gccggaacgt
     4981 ctcatgagcg tctccttacc tttaattctt ttttctcatt gagttatata tccgcaggct
     5041 atcttatcat cagtttctga ccgtgttcag tgaccgaaca acccgaacca gaccccccgt
     5101 ttcttttcct gtcccggagg gggctttctg gtatctcata taatgatttc agtaaaacat
     5161 actgaggccg gagaacggcg ccgtttgtgc tggtttttgt tgcatctgga ggggattgcg
     5221 catggtgtga gtggctgttt ttttgatcat tttcacaaaa acgcaaattt acagacttga
     5281 catgaaaaac aaggcagcgt aatttttgta ctgtatataa aaccagtggt tatatgtaca
     5341 gtattttatt ttagtttatt gttttaaaag tcaaagagga aaagtatatg agtggcggag
     5401 acggacgagg tccgggtaat tcaggtctgg gacacaatgg tggtcaggcc agtgggaatg
     5461 tgaacggtac atcaggtaaa ggtggccctt catcaggtgg tggtacggat ccaaacagcg
     5521 ggccgggctg gggtacgacg catacaccta acggggatat tcataactat aacccggggg
     5581 agtttggtaa cggcgggagt aaacccggtg gtaatggcgg taacagcggc aatcatagcg
     5641 ggagctctgg tggcggacag tcttcggcca ccgcgatggc cttcggtctg cctgccctgg
     5701 ccactccggg ggctgaagga ctggctttat ccgtttccgg cgatgcgttg tcggccgctg
     5761 ttgctgatgt gctggctgcc ctgaaaggtc cgtttaagtt tggtctgtgg gggattgcga
     5821 tttacggagt gctgccttct gagatagcaa aagatgatcc gaaaatgatg tcaaaaatcg
     5881 tgacgtcatt gccggcagac acggtaacgg agacgccggt cagctccctg ccgctggacc
     5941 aggcgacggt cagcgtcact aaacgtgtgg cggatattgt gaaggacgag cggcagcata
     6001 ttgcggttgt caccggccgg ccaatgagtg ttcctgtggt ggatgcgaaa ccgacaaaac
     6061 gtccgggggt attcagtgtg tcgattccgg gtctcccgtc tctgcaggtg agcgtaccta
     6121 aaggtgttcc ggccgcgaaa gccccgccaa aaggcattat tgctgaaaaa ggtgattcac
     6181 gtccggctgg ttttacggcc ggtggtaact cccgtgaggc cgttattcgt ttcccgaaag
     6241 agagcggaca gaaaccggtc tatgtgtcgg tgacggatgt tcttactccg gcacaggtaa
     6301 aacagcgtct ggaggaagaa aagcgtcgcc agcaggcatg ggacgctgct cacccggaag
     6361 aggggctgaa aagagagtat gataaagcga aagctgagct ggatgctgaa gataaaaata
     6421 ttgcgacctt aaacagtcgc attgcatcga cagagaaggc gatccccggt gcaagggctg
     6481 ctgttcagga agccgataaa aaggtaaaag aggcagaggc aaataaagat gattttgtga
     6541 cttataaccc gcctcatgaa tatggctccg ggtggcagga tcaggttcgc tatcttgata
     6601 aggatattca gaatcagaat gagaaattaa aagctgccca gacatcttta aacgaaatga
     6661 atgaatcctt atccagggat aaggctgcgc tttccggggc gatggagagc cggaaacaaa
     6721 aggagaaaaa agcgaaggat gcagaaaata aattaaatga ggaaaagaaa aaacctcgta
     6781 agggaactaa agattacggc catgattatt ttcctgatcc caagactgaa gatattaagg
     6841 ggttgggaga gttgaaagag ggtaaaccta aaacccctaa acaaggtggt ggtggtaagc
     6901 gggcccgatg gtatggtgat aaaaagcgta aaatttatga atgggattcc cagcacggtg
     6961 agcttgaagg gtaccgcgcc agtgatggcg aacacctcgg ggcattcgat ccaaaaacgg
     7021 gtaagcaggt taaagggccg gatccaaaac gaaacattaa aaaatatctt taagaggtaa
     7081 atatggggct taaattacat attcattggt ttgataagaa aaccgaagag tttaaaggtg
     7141 gtgaatactc aaaagacttc ggtgatgatg gttctgtcat tgaaagtctg gggatgcctt
     7201 taaaggataa tattaataat ggttggtttg atgttgaaaa atcatgggtt tcgatattac
     7261 agccacactt taaaaatgta atcgatatta gtaaatttga ttactttgta tcctttgttt
     7321 accgggatgg taactggtaa gcaaaagtta tcaaggatga tttgaaatac agacttccgg
     7381 tgttcaggga tgaataccgg ggtttttaaa tacagggaag gtatatgaaa aaggtaaagg
     7441 ttgtttttct ttttatattg attttttcag gctttcttct ggtcgcatgt caggcaaact
     7501 atatccggga tgttcagggt ggaacggtgg caccatcgtc ctcctctgaa ctgacgggga
     7561 tcgcggttca gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7621 cttttgccct gtaaacgaaa aaaccacctg gggaggtggt ttgatcgaag gttaagtcag
     7681 ttggggaact gcttaaccgt ggtaactggc tttcgcagag cacagcaacc aaatctgtcc
     7741 ttccagtgta gccggacttt ggcgcacact tcaagagcaa ccgcgtgttt agctaaacaa
     7801 atcctctgcg aactcccagt taccaatggc tgctgccagt ggcgttttgc gtgcttttcc
     7861 gggttggact caagtgaaca gttaccggat aaggcgcagc agtcgggctg aacggggggt
     7921 tcttgcttac agcccagctt ggagcgaacg acctacaccg agccgagata ccagagtgtg
     7981 agctatgaga aagcgccaca cttcccgcaa gggagaaagg cggaacaggt atccgggaaa
     8041 cggcagggtt ggaacaggag agcgcaagag ggagcgattc atcggaaacg gtgatgatct
     8101 ttaagtcctg tcgggtttcg ccactcctga ctgattcatg gttgagccac cggctcccac
     8161 agatgcaccg aaaaagcgtc tgtttatgtg aactcagtca ggagggcgga gcctatggaa
     8221 aaacgccacc tgcgtggcct tgccgttttg cctcacatgt tagtcccctg cttattcacg
     8281 gaatctgtgg gtaactttgc atgtgtccgc agcgcccgcc gcagtctcac ggccggagcg
     8341 tagcgaccga gtgagcgagg aggcgcaatt tccccggcac ctacaagacg gacaccggac
     8401 gcgctgatgt ggcatattgt cccctgatta agtctttata ttccctgata ataaattacc
     8461 aggataggtg aatcatatcg gtttttcgct gaacatttta cgttaaccta tctctgttgc
     8521 ttttttctcc ttacggagaa atacaggtca tccccgcgag ggcgtggctg acctcagggg
     8581 gatatcccct gcttacgctg gctggacgcc accgacgcac ggtctctccc cctctttctg
     8641 gctccgtccg gctggacgcc ggccacgcca gaaattcacc cggcccccgt caggctcacg
     8701 cctgaccacc cacaaaaatc cacggtgctg gacgcacctg gcgaagcagc aaagctgctc
     8761 ctgattttgc gggtggtcag ccgctcgcta tggcgaactc tgacgggggc cggaacaccg
     8821 gaaaagaccg cactggggtg ccgccttttc ctctcacacg ctggggcgtg ctcgttgttc
     8881 ctccggggct gcgccttttc cgcatggggc ggaaagaggc tggcctccgt ggtgaaagct
     8941 gaacgcaatc accacggacg tgaccgccgc cgccatgcct tggggcatgt ccgcggcttg
     9001 tgcagcgggg gctgcacatc acgccggcaa accatcgtct gaaggagaga gaacatgttt
     9061 aatacggatt cgcttgcctg gaagtggcac attccggagc tggcccggca gatttatccg
     9121 tgggtcagct ggctgtacca ggagtattac ggtctggccg gggctttcct agttatcggc
     9181 ctgtttttcg gtctggctga actttgggcc ggacggatgc gttttgtggg gctgagtcat
     9241 aaggagcgac gggagtatgc cgatcgctat aagcgcgata ataaacggcc agcg
//
