LOCUS       NZ_CP048783             3511 bp    DNA     circular CON 22-DEC-2023
DEFINITION  Klebsiella pneumoniae strain EGKP7 plasmid pEGP7.5, complete
            sequence.
ACCESSION   NZ_CP048783
VERSION     NZ_CP048783.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14082726
            Assembly: GCF_017161485.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Hamza,E., Dona,V., Elhariri,M., Elhelw,R., Hamza,D. and Perreten,V.
  TITLE     Klebsiella pneumoniae -Carbapenamese-producing, complete sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Hamza,E., Dona,V. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2020) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina
            NovaSeq 6000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/22/2023 17:16:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,727
            CDSs (total)                      :: 5,603
            Genes (coding)                    :: 5,457
            CDSs (with protein)               :: 5,457
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 54 of 146
            Pseudo Genes (incomplete)         :: 85 of 146
            Pseudo Genes (internal stop)      :: 29 of 146
            Pseudo Genes (multiple problems)  :: 19 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048783.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="EGKP7"
                     /isolation_source="chicken-internal organs from poultry
                     farm"
                     /host="chicken"
                     /db_xref="taxon:573"
                     /plasmid="pEGP7.5"
                     /geo_loc_name="Egypt:Giza"
                     /collection_date="2015"
     gene            complement(95..310)
                     /locus_tag="G4D19_RS28560"
                     /old_locus_tag="G4D19_29040"
     CDS             complement(95..310)
                     /locus_tag="G4D19_RS28560"
                     /old_locus_tag="G4D19_29040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(317..814)
                     /locus_tag="G4D19_RS28565"
                     /old_locus_tag="G4D19_29045"
     CDS             complement(317..814)
                     /locus_tag="G4D19_RS28565"
                     /old_locus_tag="G4D19_29045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            complement(<855..1553)
                     /locus_tag="G4D19_RS28675"
                     /old_locus_tag="G4D19_29050"
                     /pseudo=""
     CDS             complement(<855..1553)
                     /locus_tag="G4D19_RS28675"
                     /old_locus_tag="G4D19_29050"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1492..1833)
                     /locus_tag="G4D19_RS28575"
                     /old_locus_tag="G4D19_29055"
     CDS             complement(1492..1833)
                     /locus_tag="G4D19_RS28575"
                     /old_locus_tag="G4D19_29055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3029..3211)
                     /locus_tag="G4D19_RS28580"
                     /old_locus_tag="G4D19_29065"
     CDS             complement(3029..3211)
                     /locus_tag="G4D19_RS28580"
                     /old_locus_tag="G4D19_29065"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016265984.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     Typing          complement(1919..2092)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          2059..2580
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.69"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(4..1502)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(1499..1812)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="9.860000000000001e-59"
ORIGIN
        1 tgagataatg tgcgttgccg ttccggtacc agatctgctg tcttctctgg ttccttttcc
       61 gttgtttgcc cgtatcgctg ttgctcaatc tcattcagcc tgtcgataac cggcgcagct
      121 gctcgctcaa actctgcacc tgcctgctca agcccgacac gcgctcgctc agcgccgcgt
      181 tctcccgttg cataagacca gacatcttcc gccattcctc gaaggcgctc tcccactcgt
      241 ccagccttct cgagtagtcc tgctgtagct gctctaacgc gctcagcaat tgtttttcca
      301 gttccgtcat tctgctctac tcccgtacca gcttcaccgc gttccgcgtc ttgttgtcca
      361 ccgtccggcc cgtttcggct ttcatcccct ccggcagcac cagaaaccgt ccgttgcggt
      421 tctccagata ggtcacgccc cacgtctgca tgtgcagttt cgacagcgta tctctctgct
      481 gcctgatttc cgccaggttc gccgctatcc ggttcccctg gtaccacagc accccgctca
      541 ggctggcgaa cagcagccct gacaccatgg tgatggtcag ccacgtccgc ccgaccattc
      601 gcagcacgct cagccggttc gactgcatcg ccgcgttcat cccctgttcg tgggcgctga
      661 tggcgtcgtt gattctcttc gcgcttgcgt tcagttccga tttgacaacg ttctcgtgct
      721 tgctgaatgc ggctttcagc ctctcttcgg taagctgctg ctgtgctttc gatttctcct
      781 cgaagtccct cgaggccgtt aaaagactgc tcataaatgg ctcccctcag tcggatattt
      841 cgccccccgt ccgggtcggc aatgctgata ctgttttttg tggttctgac cacctcaaaa
      901 ccggccgctg tgagcgcccc tgtgacgtca tcacgcgttt tcagctctcc tgatgaggct
      961 aacatcagca aaccccgtgt aatcgtctct gcggcttcct gcttcgtttc tggcagtcct
     1021 gaaggcgtga ccagcgcccg ccggttctcc gggtcgttcg gatcatgtag ccccagcctg
     1081 ccgttgacca ctgtctgcca cgcatcgatg cgcggccggt cggcacgatc gtaatacggc
     1141 tggaggcgtt tccccgtcag taattcggta ttggggatca gaaaattcag ctcaagacga
     1201 cccttgtctg cgtgttccac ccacagaatg ctgtactggt ctttatcgag tccgggcatc
     1261 agaacccgct caaagctggc catcagtttt tcacgctgac ccggcggcag gtccgcctcc
     1321 gcgaatgaca gcaccccgga ggtatatttt ttcacatacg gcgacgcatc aatcagctcc
     1381 cggacttctt ccggcttccc ctgcaggacg ctggcacctt cacgctgccg gtctttaccc
     1441 agaagataat ccacaggacc ggcgcccccg ccgcgacccc ggggatggaa tttaacaatc
     1501 atcgtccggc cctttttcca gtacggcatg ccgcaaccgt tccagaccgg catcgatcgc
     1561 catcagcgct gccacgatct gcacccggtc atgaccggca ccaccggcgt tcactttccg
     1621 ggcgatctgg ttcaggttat tgcccatgcc ggcaagctga cgcagcagcg cgggcgaaat
     1681 cgagggaagc ctgccggcgc gtgtcggttt ctcatcaagg caggtctggc gcatccacgc
     1741 cgccagttgt tttccgtcgc accgctcaag caaccgccgg tgttcgtctt ccgtcaccca
     1801 catcgtgagc atcttgttgc gtttatctgc cagaatcgcc tcctgataac gccgtgacgg
     1861 acgggaaagc ctttcccggt cggcgcgggg ttggacaagc cgcaggcgcg tcagtggggt
     1921 ttcggggcgc agccctgaac cagtcacgta gcgctagcgg agtgtatact ggcttactat
     1981 gttggcacgg atgaggatgt cagtgaagtg cttcatgtgg caggagaaaa aaggcggcac
     2041 cggcgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc
     2101 tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc
     2161 tggaagatgc caggaagata cttaacaggg aagcgatagg gccgcggcga agccgttttt
     2221 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg
     2281 aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc
     2341 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttatctca
     2401 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcaca
     2461 aaccccccgt tcagtccgac tgctgcgcct tacccggtaa ctatcatctt gagtccaacc
     2521 cgagaaagca cgacaaagcg ccactggcag cagccactgg taactgggat atacgagaga
     2581 gagattcaga ggtcttgaag tatgggcctg actgcggcta cactgaaaga acagttttgg
     2641 tatctgcggt ctcgtatacc cagttacctg gttaagcagt tccccaactg acttaacctt
     2701 cgatcaaacc acctccccag gtggtttttt cgttttcaga gcaagagatt acgactgaaa
     2761 cgacaggatc acaagaagat cattaactcc gagagtagta actcccacca tgtttgccta
     2821 tcaacacact taggaaacat tacttgcgtt tagttatacg acttctagct ctccataaca
     2881 gatcctcatt ttctgctgat ggatttcgac ggtatcgttc ctccgctttc cgtactccat
     2941 attcaggaaa caccctgaca gcaaccattt gtataaagac ataacacacc aaaagtaaaa
     3001 ataaaaaacc aataaactca aacatatatc accctctttt cttctcattt ctcatgcgtg
     3061 tagccaccat tattttatag ttagcactcg tagggttctc catatattta cgggtagcac
     3121 gttcgacggc agattcggat gaactaaaaa aaagcataaa taatttaaat gcaataacgg
     3181 cgactaccac tacaccaata aatgtaagca tcactcttac tcctgttatc aacattaaac
     3241 ataacatctc ttacccgcac cgcgccggag gcactcaggg cgtttctgat ggctgacggg
     3301 caaaaggtgg tggcaagtac accgaaaacc accagaaacg cacctgattt attttacgca
     3361 tatgcatcac ttattcacat cactgcatag ccggtgattt cggttaaaaa agactctctg
     3421 tgtgctgaaa tcgcagaaaa tggccatgat ccccgtcacg ctcaggccac ctgagacggg
     3481 cgtttatcag tctccatact ccctgtcacc a
//
