LOCUS       NZ_CP048702             2746 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-E4 plasmid pEclE4-6, complete
            sequence.
ACCESSION   NZ_CP048702
VERSION     NZ_CP048702.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747521
            Assembly: GCF_015910325.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-FEB-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910325.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:52:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,574
            CDSs (total)                      :: 5,458
            Genes (coding)                    :: 5,318
            CDSs (with protein)               :: 5,318
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 44 of 140
            Pseudo Genes (incomplete)         :: 98 of 140
            Pseudo Genes (internal stop)      :: 17 of 140
            Pseudo Genes (multiple problems)  :: 18 of 140
            Pseudo Genes (short protein)      :: 1 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048702.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-E4"
                     /isolation_source="surface water"
                     /db_xref="taxon:158836"
                     /plasmid="pEclE4-6"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2015-08-04"
                     /collected_by="Public Health Ontario"
     gene            710..937
                     /locus_tag="GVI76_RS27715"
                     /old_locus_tag="GVI76_27710"
     CDS             710..937
                     /locus_tag="GVI76_RS27715"
                     /old_locus_tag="GVI76_27710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_225622915.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRACMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            complement(985..1173)
                     /locus_tag="GVI76_RS27720"
                     /old_locus_tag="GVI76_27715"
     CDS             complement(985..1173)
                     /locus_tag="GVI76_RS27720"
                     /old_locus_tag="GVI76_27715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1502..1876
                     /locus_tag="GVI76_RS27725"
                     /old_locus_tag="GVI76_27720"
     CDS             1502..1876
                     /locus_tag="GVI76_RS27725"
                     /old_locus_tag="GVI76_27720"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061335866.1"
                     /translation="MNGMSDVRLKKSTIESLKEYVNDFGMTVDDVVLNLIEENNSLKSL
                     LGQGFSLPGGDKDTHSFKDYVIHKLPKGKILVTKDGLKVSNMKKVLIEAGSAVGMDDKE
                     LNPDFTTYEIGRFLFKHLQG"
                     /name="hypothetical protein"
     gene            complement(2065..>2166)
                     /locus_tag="GVI76_RS27730"
                     /old_locus_tag="GVI76_27725"
                     /pseudo=""
     CDS             complement(2065..>2166)
                     /locus_tag="GVI76_RS27730"
                     /old_locus_tag="GVI76_27725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011847251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /name="IS30 family transposase"
     Typing          complement(2..237)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.359"
                     /coverage="82.0"
                     /evalue="7.63e-102"
     Typing          complement(2479..2746)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.424"
                     /coverage="89.0"
                     /evalue="1.46e-112"
ORIGIN
        1 cctggtatct ttaagtcctg tcgggtttcg ccaccactga tttgagcgtc agatttcgtg
       61 atgcttgtca ggggggcgga gcctatggaa aaacggcttc tccgtggcct tcttgcttcc
      121 ctgttaagct tcttcctgga agatgccagg aaatttccgc cccgccagta agccggtacc
      181 gctcgccgca gccgaacgac cgagcggagc gagtcagtga gcgaggaagc ggaatatctc
      241 cgggcttatc tttggcaccg tacgcgccat agctgcattt tttaatacga tgcaggatag
      301 ggcaaacgcc gcaaaatgac gtcatccgac atcattccgc cgcgttcgtc cagtgaccta
      361 cagtcggctc tggggtgtcg gggctgtgcc ctgaccaggc gttttggagt atcgccaccg
      421 tggtggcggt gctacaaaac atatcctgtc ccttccttgg catctctgaa gtttagccag
      481 tatacactcc gctagcgcta cgtgactggt tcagggctgc gccccgacac ccgctaaaag
      541 cactgacgcg cctgcggctt gcccaacccc gcgccgtccg ggaaagaatt tcccgatcga
      601 cccgacgttt ctgtttacct gtgatgttaa cgtttggatc tttttcccgg gtttacctat
      661 tttcttctaa tgaagcgttc acagctgacg ttaatagatg ggtgctttca tgtttggttt
      721 ttcaccagga gaacttatga acctttgccc cgatgaacgt ctgctctttg tgcggatgat
      781 ttccgctatg cttcgccgtt cgggcggtga tgcgggtgct gttatgtttg aggcgtatcg
      841 ccatattgtg tccgatacca atcaagctcg tcgtgcctgc atgctcgacc tgctcgaaag
      901 cgtcaggcat gactatgtac acggtggata tacatagttg ccctggatac gcttttcgtc
      961 taagtgagcg ttcttttcgt ctctttattc gccgtcctgt ataccgaagc gtgcgctcag
     1021 cttctgataa agcgcttctg cctgttcgtg cattcgctca cagtcggctg cctcgtcatc
     1081 aagatcgagc acgtcgagtt tttcgagcag cagcagcgac tgcgcccgga taaattttgc
     1141 catgttcagt gcggctgatt cctgttttgt catacggctt tttcccggtt ttatggtgat
     1201 cgcagactga aatcttactt cattcacttg agtgcatagc catgcaggta cgcagaaatc
     1261 atcagcgcgt ttctgagcgg ttttcagggg gtaagatgtg ttcttttgca ggtgaggtcg
     1321 tagaaacgcc ctgagagcct ctcaggcggt gcacgcaacg gatgttatgt tcaatctggc
     1381 ggtgcggcat agttatcatc gcacctacag accgcttgtt gtattcagaa caatatttat
     1441 ttgtataatt tgtattttgt tgtattattt cttcaaatta gagcggtggg gtaataataa
     1501 tatgaatggt atgtctgacg taagactaaa gaaatcaact attgagagcc taaaagagta
     1561 cgtgaatgac tttggcatga cggttgatga tgttgtcctg aacttaattg aagagaataa
     1621 ttcattaaaa tcgttattag gtcagggctt ttctttgcct ggtggagata aggacacaca
     1681 ttccttcaag gattacgtga ttcataagct tccaaaagga aagatcttgg ttaccaaaga
     1741 cgggcttaaa gttagcaaca tgaagaaggt attgatcgag gcagggagcg ctgtagggat
     1801 ggatgataaa gagctaaacc ctgactttac gacctatgaa ataggccgtt ttctcttcaa
     1861 acatcttcag ggataaaggt gtctaccagt tcgtggccgg gattttaggt tcaattcagc
     1921 tagttcccgg acagaaaaaa agccaccgtg taggtggctt ttttattcct gctttaggca
     1981 aagcattatt gatagcattt gcaagcaaac cgttagcgac ttctgctgaa tgcttctggg
     2041 cggattcaag ttcgaagtgc aacgctaagc cgcctgtctt agctcagcaa agaccactga
     2101 aggttgccgg aatccaagac atttccgtgg cctgctattc agtgctctct ggtacttgcg
     2161 gagctcccct gtgtgataag tcaggtgtgg aatttttacg tataaggccc cgctaggcgg
     2221 aaaccaatac tgaagccaaa tcactggtaa acagccggat cttcttgtga tccttttact
     2281 ctcgtcgtaa tctcatgtcc tgtaaacaaa aaaaccaccg ctaccaacgg tggttcgttt
     2341 gccggaatca agagctacca actctttctt ccgaaggtaa ctggctttca cggagcgcag
     2401 ataccaaaac tgtccttcca gtgtagccgc agtcaggccg ccacttcaag aactctgtag
     2461 aagcactcta ctaccctgct ccgtgaatcc tcttaccaat ggctgctgcc aatggcgtta
     2521 agtcgtgtct ttccgggttg gactcaagac gatagttacc ggacaaggcg cagcggtcgg
     2581 actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga
     2641 gtgtcaggcg tggaatgaga taaacgcggc cataacagcg gaatgacacc ggtaaaccga
     2701 aaggcaggaa caggagagcg cacgagggag ccaccagggg gaaacg
//
