LOCUS       NZ_CP048669             6078 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Acinetobacter piscicola strain YH12207_T plasmid pYH12207-10,
            complete sequence.
ACCESSION   NZ_CP048669
VERSION     NZ_CP048669.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13979601
            Assembly: GCF_015218165.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter piscicola
  ORGANISM  Acinetobacter piscicola
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Chen,C., Sun,J., Liao,X.-P. and Liu,Y.-H.
  TITLE     Tigecycline-resistant Acinetobacter species from pigs and migratory
            birds
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Chen,C., Sun,J., Liao,X.-P. and Liu,Y.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2020) College of Veterinary Medicine, South China
            Agricultural University, Wushan, Guangzhou 510642, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 11:34:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,216
            CDSs (total)                      :: 4,108
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 108
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 67 of 164
            Pseudo Genes (incomplete)         :: 118 of 164
            Pseudo Genes (internal stop)      :: 42 of 164
            Pseudo Genes (multiple problems)  :: 51 of 164
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048669.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Acinetobacter piscicola"
                     /mol_type="genomic DNA"
                     /strain="YH12207_T"
                     /isolation_source="feces"
                     /host="Pig"
                     /db_xref="taxon:2006115"
                     /plasmid="pYH12207-10"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="29.08 N 119.65 E"
                     /collection_date="2017"
                     /collected_by="Chong Chen"
     gene            complement(72..740)
                     /locus_tag="G0028_RS20975"
                     /old_locus_tag="G0028_20945"
     CDS             complement(72..740)
                     /locus_tag="G0028_RS20975"
                     /old_locus_tag="G0028_20945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     gene            complement(873..1358)
                     /locus_tag="G0028_RS20980"
                     /old_locus_tag="G0028_20950"
     CDS             complement(873..1358)
                     /locus_tag="G0028_RS20980"
                     /old_locus_tag="G0028_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     gene            complement(1467..3281)
                     /locus_tag="G0028_RS20985"
                     /old_locus_tag="G0028_20955"
     CDS             complement(1467..3281)
                     /locus_tag="G0028_RS20985"
                     /old_locus_tag="G0028_20955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3271..3678)
                     /locus_tag="G0028_RS20990"
                     /old_locus_tag="G0028_20960"
     CDS             complement(3271..3678)
                     /locus_tag="G0028_RS20990"
                     /old_locus_tag="G0028_20960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4681..5367
                     /locus_tag="G0028_RS20995"
                     /old_locus_tag="G0028_20965"
     CDS             4681..5367
                     /locus_tag="G0028_RS20995"
                     /old_locus_tag="G0028_20965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     gene            5458..5991
                     /gene="aadB"
                     /locus_tag="G0028_RS21000"
                     /old_locus_tag="G0028_20970"
     CDS             5458..5991
                     /gene="aadB"
                     /locus_tag="G0028_RS21000"
                     /old_locus_tag="G0028_20970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     ARG             5459..5988
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             5447..5991
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          complement(1471..3281)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctactaacct tgttaattca acatttgcaa aatagtctga cctttcgatt gtttggggtg
       61 aaattcttat cttaaaaatc taaatattct tttagatcta atgcagattg ctcgataaaa
      121 tcgtatgtat taatacaacg acagtaaata aattttgtta aaacctctaa atcattttct
      181 agattacggt taaaaataat cggttcatga tgagcaatcc tgtttcttaa ttcacgtaat
      241 tgatctgtcc aatcgtaaaa aatttgcctg ttttcaactg ttgcattagg aaaaatagaa
      301 ttaaaacatc cctgccaaat acgttcttca taattagcca taagcatctt ttgccagaat
      361 acaaatttaa gctcagggat cattttacta ggcgaatctg ggaacttctc tttagcacta
      421 tcaaacatac ttttagggct atacctccct ctacggctaa ggcttcgaat aaaagcttca
      481 ttttcatgcc aactatttgt ttcattacac ctaaaagata tttctattgc ctgaacaaca
      541 gcatttctca gaacaacttc aaaataattt aataagtaaa aaaacttatt ggaaattatt
      601 aaattacgtt cataaagcat caatacatca gatttttcat atggttcacc tttatcttta
      661 tagaaatttt catacttaga taagcgtggt atagataaag cctgctctat cttaagcact
      721 gcatcatttg acaattccac cgtatatacc ctaatatata gttgtaagtt tgagagactg
      781 cggatctaag atcttccctc ccaagcgcta agattcaaaa aagcctttca ttaacgtgaa
      841 aggctttttt ctttttttgg tcaattaaaa gcttacatat tgattgtaat tgaaatattt
      901 tcttgaggga catcaaaata attagctaat agtctcttag cttcaggaat agatatttta
      961 tccagtgttt tagaagaatt atcttcattt aatttggtaa atttcagctc attcaaatcg
     1021 atattcatct cttctaatga actataagaa actggattcc gattaccatc ccacatattg
     1081 ggaatagata tatcagcata ttcggagata tttattatcc atctaccatc tgcatcttct
     1141 tcttgagatt ggctgatact agatattttg ccaactaaaa atgcagtgtg atgatcagcc
     1201 tctggttctc cccaagattg tttcctattt tgcacacaaa ctagataatc gcactttcct
     1261 gcattatgag catttagtct ccaagactga ctaccacctt cttttaaaat tctagtcttt
     1321 ccacgagcag ttagtactac tacacagtta ttattcataa caccaccaaa atatataaac
     1381 aagcctatac tacattcaat atagctaaat ttaaacatta gtacaagcta atactaagaa
     1441 ataaacacca acatactaga aaataattac atcccaaaat ccatacctcg atcttgctta
     1501 ggtcgaggtt tttctatctc ttgtttacgc tcattcatct ggacgagatt tttttcaata
     1561 tctcggatgt ttcgagcaaa ttctgctgcg ctttggctac ttctgacgag acagaatgct
     1621 gtgtggttga gattctctcc agaatttctt cgttgttggc tgtgataagg ctcatctctc
     1681 ggactttgcc attgatctgc tcggtatagc ttgctgtact tcgttctagc tcgtgaaatt
     1741 gcttctgcat tgtcgctaat gatttctgtg cttgctgtaa gctttgctga tcgctcttga
     1801 ttgtctttga ttgtgctgac aaggcttgga taattttggt ttgcattgtg tctaaagctt
     1861 gctgttgctt ctgcaattgc acttgttgtt gcattaattg ttgctgttgt tcttcgatga
     1921 ttctcaataa ttgtgtttct aattcggtca tcgtttaata ccccattgga atcatgtagc
     1981 cgttctgcca ttcgcctgcg ttcttgttga tctgaataca taggcgctgt gctttgctgt
     2041 ctggtcgctt gcatgtgctg atcgtcaaac tgcctcccga tcggttcaaa tggtcgaccc
     2101 tcatttgctt ctcgttgatc acggtattta gcgtctgcat ctcgctgtac ttgtttttgg
     2161 tgacgagacc aagcgagaaa attgctataa gcaaaataac tgctactgaa atcagtccca
     2221 tattcaaagt ggtaagtttt ttctgtaccg cttgattctg ggcttgttgt tcttggctct
     2281 gtatttgctc gtttaattgc gttaaatggc tcaatttcga ttgcagcaag gtttgataca
     2341 ggcttaactg cttctcgacc gtctgggcgt tgttttgaat gctctgggct attttctcgt
     2401 ccgactgttg gatagcggtc gcagtgatat tggctcttat gttcaagctg tctctgatag
     2461 tctgtgacgt ttgttcgata ccgctcagaa gttgttcctc tgtattcttc aattcgttgt
     2521 tgtacttctt gtctatattc gccagtagct ctaaagcctt gctcataaat ctcgccctct
     2581 aaacggatgg gacgttttgc gccctcatat gggttttcaa tggatatgga gttttttact
     2641 tgtcgagtga cattgatttg attcgattca agccattgca ccaattcttg ccgatcggca
     2701 acatcaccat tggcaaccgc tcggtaaaca cgttgatgta actgcgcttt aaaatcctta
     2761 atatctttgg gtaggttctt tttatttaaa aatagctgtc gatgctcagg atcatcgggg
     2821 tcatacagtg aatgttcggt attagtgatc tgcttaaata gatcgactcg atctaaatcg
     2881 actggcgcat agaatggctg taggcgtttg cctgttgaaa gctcaacatt aggaatcaca
     2941 aaattaagct caagccttgt ctcacctgtg tcctgattaa ttttgtcttg gtgctgtacc
     3001 cataaaattt gatattggtc tttatctagt cccgggaata aacattcctc aaaattttgc
     3061 atgattttct gtttatcctg gtcagataaa tcatgctcat aaaaggacaa acaaccactg
     3121 gtatattttt tggcaaaagg actgctatca atgagttgtg ctgtcagttc tacatcacca
     3181 ttgagcactt gagcatgttc acgctctcga tcttcaccta acaaataatc gagacagcct
     3241 tttgataaac ctgaaccatg tctaaagaaa tcaacgatca tctggacgta actcccgaag
     3301 ctgctgatct atggaaatca acaaatgaag taacttcact ttgtcaaaag tacctacccc
     3361 tgcaatatcg gtattcactg ctttagcaat ctgattcaca ttgttgccaa tccgaccaag
     3421 ctctaaaatt aatgctcgct ctgtcttgct atacgaagat tcaacaacct ttgttgcttt
     3481 aattttttgg tcatcttctg ctttggaatt atgaatttta gtcgctttct ctactgctgc
     3541 ttgtctaagc aactttgcaa tcgaaccata aggattattg gaatcgagca attgatattc
     3601 tgattctgaa agtctgactt taaaggttcg ttctcgaact gctttgctct gtttgttttc
     3661 ttgttcctgt cctgacattt caatctaccc tgctttggct tttgtaagct ttaaattgga
     3721 aaatctttga tttttctgtt taaagctttt cgggggtatc gggggtgaag cccctgatca
     3781 aggtcacaga ctatcactgt ttaacgaagt taaatagggg tagctgtgta tccttgctta
     3841 tttaaaaatg gacgagtgaa attcaataaa aactaatcaa atgacacggt atataaactc
     3901 tagtaggtat tggcatgaat cttgtaaaaa caaatgcgct caactctttc tcaccgtccc
     3961 cacatccctc gctttgctcg ggctgatggt gaacgttgag agagtttcgg caatactttc
     4021 cctaagaggc tttgatttaa aaaaatcaaa tatcctctta agtattgccg attaacctgt
     4081 aattgggctg acggagccac gcatcggtcg tatccgcaca tggttttgct gaccatgcag
     4141 acaaggacta aaggttttgc accttctccc acgcgctccc tgtcgaagta tggtggtatc
     4201 tctgcgagga ttactccaac atgcaactta accaacctat acaactactt ccaacgctcc
     4261 gcctatgtga gatcccctgt aagcggttta gccttgcgac tatcgctacg atgcaccagt
     4321 gacacttatt ccctccacat gcaacgcata tcttccttaa aaaaggcgca cagaagtaga
     4381 taaagctcga ccgttaggtg cttatttgat gatttaaact gctgaattaa cgaaggactt
     4441 caaattgagg cttgtcaaac tgttctaatt ctttaaaata gatagtggta ctagccttta
     4501 aggtgaatat cagtatgagt tgcaagctct gccgatcttt cgttaattga tgttgcaagc
     4561 atcaaataac tgtacccatc gaatttaaaa ctaatggaca gatttcgcaa gagacctgtc
     4621 cattattttt tttgattaca cctaagaatt aagctgaaac tcctattctt tggactatta
     4681 atgtcaaaac ttcctagatt tcataacaag cacatcgaaa aacgtaagta ttcaaatagc
     4741 ctcctagcca aatctatttc ttctaaagta cgtttctcta ataaaacatt ttttaatgtt
     4801 aatttgagag gatcccgcct acgatcatgt tcattcaaaa atataaaaat tgttttatct
     4861 gatttttcag gagtaatctt aaataagaat aggtttacga atgttcactt caaaaagtgc
     4921 gtattttatg ctgctatttt tagagattgt cgctttaaaa attgcacatt tgaaaaatgc
     4981 atttttatta atacaaatac agaaagccta aatcactcga tattagactg ttctaaaaaa
     5041 tataattttt acgaaatgaa aattcctgat gacttaaaag atcaactaac agaatataga
     5101 aatattccaa tactgcaaaa aaaccgttta cttcatctaa aagggggaag aatcaatacc
     5161 gcaaccttat tcattctcct atccaaaata tccaaataca agctgctaaa tggtcttaaa
     5221 aaaatttata ttgaggaaca aagtaaagta aaaatactta gcactttcaa actccttgaa
     5281 gaaataaaga acctagttga cactaacacc gcattcaaac agaataatgg tgctctgccc
     5341 catccaatcg aaaggttgga tagttaagac aatcactggg agctcactat tggatgtaac
     5401 aattcaatta atagtctaat tactgacatt ctgggagggc ttactatgtt aggccgcatg
     5461 gacacaacgc aggtcgcatt gatacaccaa attctagctg cggcagatga gcgaaatctg
     5521 ccgctctgga tcggtggggg ctgggcgatc gatgcacggc tagggcgtgt aacacgcaag
     5581 cacgatgata ttgatctgac ttttcccggc gagaggcgcg gcgagctcga ggcaatagtt
     5641 gaaatgctcg gcgggcgcgt cacggaggag ttggactatg gattcttagc ggagatcggg
     5701 gatgagttac ttgactgcga acctgcttgg tgggcagacg aagcgtatga aatcgcggag
     5761 gctccgcagg gctcgtgccc agaggcggct gagggtgtca tcgccgggcg gccagtccgt
     5821 tgtaacagct gggaggcgat catctgggat tacttttact atgccgatga agtaccacca
     5881 gtggactggc ctacaaagca catagagtcc tacaggttcg catgcacctc actcggggcg
     5941 gaaaaggttg aggtcttgcg tgccgctttc aggtcgcgat atgcggccta acaattcgtc
     6001 caagccgacg ccgctttgcg gcgcggctta actcaggtgt taggaaacac tgctaccgtt
     6061 atcggattta ctgcttta
//
