LOCUS       NZ_CP048666             8939 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Acinetobacter piscicola strain YH12207_T plasmid pYH12207-7,
            complete sequence.
ACCESSION   NZ_CP048666
VERSION     NZ_CP048666.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13979601
            Assembly: GCF_015218165.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter piscicola
  ORGANISM  Acinetobacter piscicola
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 8939)
  AUTHORS   Chen,C., Sun,J., Liao,X.-P. and Liu,Y.-H.
  TITLE     Tigecycline-resistant Acinetobacter species from pigs and migratory
            birds
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8939)
  AUTHORS   Chen,C., Sun,J., Liao,X.-P. and Liu,Y.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2020) College of Veterinary Medicine, South China
            Agricultural University, Wushan, Guangzhou 510642, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 11:34:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,216
            CDSs (total)                      :: 4,108
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 108
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 67 of 164
            Pseudo Genes (incomplete)         :: 118 of 164
            Pseudo Genes (internal stop)      :: 42 of 164
            Pseudo Genes (multiple problems)  :: 51 of 164
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048666.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8939
                     /organism="Acinetobacter piscicola"
                     /mol_type="genomic DNA"
                     /strain="YH12207_T"
                     /isolation_source="feces"
                     /host="Pig"
                     /db_xref="taxon:2006115"
                     /plasmid="pYH12207-7"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="29.08 N 119.65 E"
                     /collection_date="2017"
                     /collected_by="Chong Chen"
     gene            complement(join(8658..8939,1..648))
                     /locus_tag="G0028_RS20840"
                     /old_locus_tag="G0028_20815"
     CDS             complement(join(8658..8939,1..648))
                     /locus_tag="G0028_RS20840"
                     /old_locus_tag="G0028_20815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_180097431.1"
                     /translation="MAIARLSVKVGKAGKAAPHAEYIDRDEEKKKKEEQAKIDLEHSDY
                     GNMPKWAEHNPINFWQAADLYERKNGSTYREYEIALPREMNAKQRLELVEDFIQSEIGS
                     KYPYQFAIHNPKAMDGNDQPHVHLMFNERLQDGIERDPEQYFKRYNSKNPERGGAKKDN
                     TGKSYQERKTDIKDLRQRWADLCNSHLEKHQIDSRIDMRSYKEQGIDKEPEKKLLPSQA
                     KNPEIREALQQSRTAHKELERLDLGDPKKDLKDLKDSPISDKEIKQGIESFKADFDSFK
                     QLALEQYKEQQKLEREQQKTMSYKGMSR"
                     /name="MobA/MobL family protein"
     gene            complement(648..803)
                     /locus_tag="G0028_RS20845"
     CDS             complement(648..803)
                     /locus_tag="G0028_RS20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_180047840.1"
                     /translation="MQLKQVLGKIKDIIFGVIAAPLLIIIMVLASILDQRAFKKERNQK
                     KEQDKT"
                     /name="hypothetical protein"
     gene            1084..1491
                     /locus_tag="G0028_RS20850"
                     /old_locus_tag="G0028_20820"
     CDS             1084..1491
                     /locus_tag="G0028_RS20850"
                     /old_locus_tag="G0028_20820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005207259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_180047842.1"
                     /translation="MNNDWLSEKITYISALKNPSDAQKLLLELAKIQYRTPDQEKKLNA
                     LIKAEKAIDRANKQKVAVRKLLNAEKEAERKARTRHLIQLGALFEIANLDERDPAELLG
                     VLLKTAEIDPNDMKWQIWKDLGQDVLNQRKK"
                     /name="conjugal transfer protein TraD"
     gene            1734..3722
                     /locus_tag="G0028_RS20855"
                     /old_locus_tag="G0028_20825"
     CDS             1734..3722
                     /locus_tag="G0028_RS20855"
                     /old_locus_tag="G0028_20825"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011234839.1"
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA];
                     GO:0050151 - oleate hydratase activity [Evidence IEA]"
                     /GO_process="GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="WP_180047844.1"
                     /translation="MAKKTIAKFDISATAPEGFEHEPDATGGFWCHKPLNTLPPGDFIS
                     PYSRHKTLPTPGIEKRKAWIIGGGVGGLAAAFYLIRDGHMPAENITILEEMRVEGGSMD
                     GAGNPEDGYIIRGGREMNWNYDTLWDLFQDIPALELPEGYSVLDEYRMINDNDPNYSKA
                     RLINKSGEILDSEDLGLSKSQQWELIRLMLKRKEELDDTSIEEYFSEGFLQSNFWFLFR
                     TMFAFKNCHSLLETKLYLHRFLDSIDGFGDMSVLLFPKYNQYDTFIKPLTDFLREKGVK
                     FTFEARVDDLDISFTGDKKTVTGIQAVIKGKEQYIEVGKNDLVFALTGSMTEQTGYAGM
                     DNAPELNCERHDPGSESGWNLWKNLAKKSPVFGKPEKFYGDVDKSIWESATLTCKPSPL
                     VDKLKTLSVNDPYSGRTVTGGIVTFTDSNWLLSVTCNRQPHFPDQPDDTLVLWVYGLLM
                     DKQGNRVNKTMPECTGKEILTELCHHLGIEDQLDEVIANTKIRIALMPYITAQFMPRAA
                     GDRPRVVPEGCTNLGLVGQFVETRNDIIFTMEASIRTARIGVYKLLNIPKQVPDISPTQ
                     YDIRNIIKGARALNSNKPFIGERMLHRLLDKTYFAHILPPLPEPEETKQTHFEEELREL
                     LGKGGDSIHKFSSWVNSLRENFSSKDK"
                     /name="oleate hydratase"
     gene            3960..>4685
                     /locus_tag="G0028_RS20860"
                     /old_locus_tag="G0028_20830"
                     /pseudo=""
     CDS             3960..>4685
                     /locus_tag="G0028_RS20860"
                     /old_locus_tag="G0028_20830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074120.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /name="IS256 family transposase"
     gene            <4946..5245
                     /locus_tag="G0028_RS20865"
                     /old_locus_tag="G0028_20835"
                     /pseudo=""
     CDS             <4946..5245
                     /locus_tag="G0028_RS20865"
                     /old_locus_tag="G0028_20835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612260.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(5259..5759)
                     /locus_tag="G0028_RS20870"
                     /old_locus_tag="G0028_20840"
     CDS             complement(5259..5759)
                     /locus_tag="G0028_RS20870"
                     /old_locus_tag="G0028_20840"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_180047846.1"
                     /translation="MNKLNILLLSFLLTACGNSTTEPKEQQEFKSLISSLSNEYKTAKN
                     SDNDLEIKDFENKFKKELKDTNRAVSSWVGSFVSSTLNNDKLTIELSNGDQTYYLILVD
                     PKVIEYSKKFKKDDQILFSGNLGNESSLTINGALSEPEFRFYPTKISTVDDKDGIIQSQ
                     KII"
                     /name="hypothetical protein"
     gene            complement(6590..7399)
                     /locus_tag="G0028_RS20875"
                     /old_locus_tag="G0028_20845"
     CDS             complement(6590..7399)
                     /locus_tag="G0028_RS20875"
                     /old_locus_tag="G0028_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_180047848.1"
                     /translation="MLKRSSLQRKEFKQEELALMKKLDLNDIEIIKSDNLAQLLIWTSE
                     LSQHNHAQFIEFSNRLENVDEIEEREEINKKITFYRNSAFSENQIKDFYLKKLLDQGEA
                     TVRGYIKHRIKPDMKYAVISYHGYDFISFATPEIIEKFPLNFIDFVQMEAPKTLADIHP
                     DEALLLSSIKDFIKPFKEQYKKITSKNRDIKEHNAVVHEIYIKIKNKEATLQKSTDSKP
                     KESSPKSTENSAVPVEMKQVAIQKNMAPLEKPITVIKKRKFALNKEK"
                     /name="hypothetical protein"
     gene            complement(7457..7738)
                     /locus_tag="G0028_RS20880"
                     /old_locus_tag="G0028_20850"
     CDS             complement(7457..7738)
                     /locus_tag="G0028_RS20880"
                     /old_locus_tag="G0028_20850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family toxin"
                     /protein_id="WP_005098405.1"
                     /translation="MAKRKIIVTSSFKRDIKRRYLDLVTAEWAEVLDCLVANSPLPEKY
                     LDHPLKGGAEFKNCRDCHVKPDLVLIYRLVGDDVLELHQLDSHSEIFG"
                     /name="type II toxin-antitoxin system YafQ family toxin"
     gene            complement(7731..8024)
                     /locus_tag="G0028_RS20885"
                     /old_locus_tag="G0028_20855"
     CDS             complement(7731..8024)
                     /locus_tag="G0028_RS20885"
                     /old_locus_tag="G0028_20855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004973743.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_005098404.1"
                     /translation="MSAVNFNMRLEAELKEKVTPILEDYGLTMPQAFKLFLNQIVKTKT
                     IPLSFDYARETALTPKAAAKLLQSLKEIENGEYTEYETVEEAIQAMSEEANG"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            complement(8161..8661)
                     /locus_tag="G0028_RS20890"
                     /old_locus_tag="G0028_20860"
     CDS             complement(8161..8661)
                     /locus_tag="G0028_RS20890"
                     /old_locus_tag="G0028_20860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_180047850.1"
                     /translation="MTPDEKKLYALMAVAEQQQNLVNQAVKELQITEERIQKVIAGQTH
                     HTVSKSLNTGLQEGTAELIKTAKALGRLNNSIELASDQFSWKLIAIVLGIFAILMLAMT
                     WLFSVYIKPLGNIPQIESLRQQGIQFDIGSCKVNGESKTCVRVMKEQCGYGNDKDFCVI
                     DPK"
                     /name="hypothetical protein"
     Typing          complement(8662..8939)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.323"
                     /coverage="100.0"
                     /evalue="2.86e-30"
ORIGIN
        1 tttcttttca ggttctttgt caatgccttg ctctttatag cttcgcatat caatacggct
       61 gtcaatttgg tgtttttcta aatggctgtt gcataaatct gcccatcttt ggcgtaggtc
      121 tttaatatcg gtttttcgtt cctggtaact cttgcctgta ttgtcttttt tagctccgcc
      181 acgttcagga tttttgctgt tatagcgttt gaaatattgc tctggatctc gttcgattcc
      241 atcttgcagg cgttcattaa acatgaggtg gacgtgaggc tgatcattgc cgtccattgc
      301 tttagggtta tgaatggcaa attgatacgg atattttgag ccgatctcgg actgaatgaa
      361 atcctcaacc agttctaggc gttgcttggc attcatctcc cttggtaaag ctatttcata
      421 ttctcggtag gtactgccat ttttgcgctc gtaaaggtcg gctgcctgcc aaaagttaat
      481 cggattatgt tctgcccatt tcggcatgtt tccatagtca ctatgttcaa ggtcaatttt
      541 agcctgttcc tcttttttct ttttttcttc atcccgatct atatattcgg catgcggtgc
      601 tgccttgcct gccttaccga ctttgacact taaacgagca atcgccatta ggttttatcc
      661 tgttcctttt tttggttgcg ttccttctta aacgctcttt gatcgagtat cgaagcaagc
      721 accataatga tgatgagtag cggtgctgcg atcacgccaa aaataatatc tttgatcttt
      781 cccaatacct gttttagttg catcttgttg ctcctagctt tggtacttct ttttttcatt
      841 caattttcaa ttgaacgaaa aaaagaagtt tcagggtttt agggatgaag tccctaacgt
      901 gcttaggttt ctcaatgaaa ttgagaaacg tctaagcgcg ccatcaaatt tttgatggaa
      961 gtgcaggcga caatctctag aacgacttta acaaaattac gttactcgga cgtaattatt
     1021 ttgttatcgt tctttgagta ttgagcctgt acttttgcgg tatctgaatt aatggaattg
     1081 aatatgaata atgattggct ttctgaaaaa atcacctata tttctgcttt aaagaatcca
     1141 agtgatgctc aaaaactttt attggaatta gcgaaaatcc aataccgtac acccgaccaa
     1201 gagaaaaaac ttaatgccct gattaaagct gaaaaagcca ttgatcgagc gaataagcaa
     1261 aaagtagctg ttagaaaatt actgaatgca gaaaaggaag ctgaacgaaa agctcgtaca
     1321 cgacacctca tccaattagg cgcacttttt gaaattgcaa acttagatga aagagatcct
     1381 gccgaattac tgggagttct gcttaaaact gctgaaatcg accccaatga tatgaaatgg
     1441 caaatttgga aagatttagg acaagatgta ttaaaccaac gtaagaaata aaataataaa
     1501 aactatgatt ctatctagtg ataccaatag atagaatcat agttaaactc ttaattactt
     1561 atcgagaagc cgttctagga tgatactgta ttgttcatct acattttctc agaattttga
     1621 acaccctcca ttaattgact aagatggcga ttcaactaaa ctgaaagtgc tgttcgcaat
     1681 accactaagg gtatcagcga gaacagcact ttcagtaaag gaaagagtcc gtaatggcta
     1741 aaaagacaat tgcgaaattt gatatctcgg caaccgcacc agagggtttt gaacatgagc
     1801 ctgacgcaac aggtggtttt tggtgccata aaccgcttaa cactttgccg ccgggtgatt
     1861 ttatatcgcc ctactcgcgt cataaaacct taccaactcc gggaatcgaa aagcgtaaag
     1921 cctggataat tggtggcggt gtcggaggac ttgccgctgc attttattta attcgcgatg
     1981 gtcatatgcc cgccgaaaat atcactattt tagaagaaat gagggtagaa ggcggctcta
     2041 tggatggtgc gggtaaccct gaagacggtt acatcattcg cggtggtcgt gaaatgaact
     2101 ggaactacga caccctctgg gatctattcc aggacattcc cgcccttgaa cttcccgaag
     2161 ggtattctgt tctcgatgaa taccgaatga taaacgacaa cgacccaaat tactctaagg
     2221 cccgtttaat taacaagtcc ggcgagatcc ttgactcaga agacctggga ctgagtaaat
     2281 ctcagcaatg ggaacttatc cgcttaatgc tgaaacgaaa ggaagagtta gacgacacga
     2341 gcattgagga atatttcagt gaaggctttt tacaaagcaa cttctggttt ttattccgta
     2401 ctatgtttgc ttttaaaaac tgccacagcc tgttggaaac caagctctat ttgcatcgtt
     2461 ttctggactc aattgacggc tttggtgaca tgtctgttct gcttttccct aaatacaatc
     2521 agtacgacac ctttataaag ccactgaccg actttttgcg tgaaaaagga gtgaaattta
     2581 cctttgaagc cagagtcgat gaccttgata taagctttac cggagacaaa aagacagtaa
     2641 ccggaattca ggccgttatt aaaggtaaag agcaatatat tgaagttggc aagaatgatt
     2701 tagtgttcgc cctcaccggc tcaatgactg aacagacggg ttatgccggt atggataatg
     2761 cgccggagct taactgtgaa aggcatgacc cgggtagcga aagtggctgg aacttatgga
     2821 agaacctcgc caagaagtct cccgttttcg gtaagccaga aaaattttat ggtgacgtag
     2881 acaagtctat atgggagtcg gccaccctta cctgcaagcc ctcacccctt gtcgataaac
     2941 tgaaaacctt atcagtaaac gacccatact ccggtcgaac cgtaaccggc ggcatagtga
     3001 cttttactga ctccaactgg ctgttaagtg ttacctgtaa tcgccaaccc cacttccccg
     3061 accagccaga tgacacattg gtgttgtggg tttatggcct gttgatggat aagcaaggca
     3121 accgtgtaaa caaaaccatg ccagaatgca ccggtaaaga aattctgacc gagctttgcc
     3181 atcatctggg tattgaagac cagttagatg aggttatagc caacaccaaa atacgcattg
     3241 cgcttatgcc ttatattaca gctcagttta tgcccagagc tgcaggcgac aggcccagag
     3301 tagttcctga aggctgcacg aatttaggct tagtgggaca atttgtagaa actcgcaatg
     3361 acataatatt caccatggaa gcctctattc gcaccgcccg tattggtgtg tataaactgc
     3421 tgaatattcc caaacaggtg cctgatataa gcccgacaca atacgacatt cgtaacatca
     3481 taaaaggtgc cagagcgctc aatagcaata agccttttat tggcgagcgt atgctgcacc
     3541 gcttactcga caaaacctac tttgcccaca tactgccgcc gctgcccgaa ccggaggaga
     3601 caaagcaaac gcacttcgaa gaagagctaa gggaactgct tggtaaaggc ggtgactcaa
     3661 tacataaatt ctcaagctgg gttaactcgc tgagggagaa tttttcgagc aaagataaat
     3721 agtaggttag gttttatttt ttgcacaaaa aagccgctcg aatgagcggc ttcttatatg
     3781 gaattggtgg agctgtttat aaaagccttt tgtaaacaac cgatcgatca ggaatacaaa
     3841 aggtttaagg aatgcgagcg ataactttta tttcaaagtc aaaaccggct aaccaagtca
     3901 cacagtagtg ttcagaattc tgtgtcattt cgttaaacta aacaaaaaag agatgacata
     3961 tgaccaaaac caatttcgac atggatgccg ctttaaagca gttgcgtgaa ggcaaagacc
     4021 tcaccggcaa agacggcatc ctgacaccgc ttatcaaaca actcaccgaa gccgcgatgc
     4081 aggctgagct tgaagagcat ctcagtgata aagagcaatc taaccgcaag aacggttcga
     4141 cgtcaaaaac agtcaagagt cctgccggca gctttgaatt agatacgccc cgcgacagag
     4201 ccggctcgtt tgagccgcaa ctggttaaaa aacatcagac gcatttaacc gatgagctgg
     4261 agcgtaaaat tattgcattg ttttcgcttg gaaccagcta tcaagacatt cgcatgcaca
     4321 ttgaagatct gtacggcatt catgtctcaa acgtcaccat taataccgtc acggataagc
     4381 ttctgccgga gcttcaggca tggcgtgaac ggcctttaga agcggtttac cccattgtat
     4441 ggctcgatgc cattcactat aaaatcaaag agaatgggcg ttttatcagc aaagctgttt
     4501 ataccattct tggcctcaat attgacggta aaaaagagct tctggggctt tatttatccg
     4561 agtcagaagg cgctcatcac tggctaagtg tgttgaccga tctacataat cgaggcgttc
     4621 aggatattct gattgcctgc gtagacggct taaaaggctt tcccgaagcc atagaaagtg
     4681 tttttgagta ataaagttaa atactaagta ataaacaata atacttggta ataaagtttc
     4741 atcccgattt tcaaaaaaag aaaatatatt cattattttc agcatattgg aatattggta
     4801 tgaaactttt ttattttaat agaattaaac ttagattagt ttttgcgtat agtatgatac
     4861 tttaataaat atcatttaaa tttaatatca ataaaataaa acacttacac acaacatagt
     4921 atgacacttt attgctttca tataattgtc ctttgagggt tttcatgaat tacattcaag
     4981 tcattggaaa atagagcaat atcaccgggt gattaaacag gtttgtcata ttgaaaagtt
     5041 tcaagtaaga cgatctaaac tgattttgaa tcatattttt tcagccttga tggcctacgt
     5101 cgagatacaa aagaaccagt ttgagcggat ctttgaaaat gtatatcgtt ggcagaagaa
     5161 attatttaga ccagttatca aaaacttcat tgatgacttt attctcgata aaaatcatct
     5221 gctaccacag agaatctata aataatagtg cgtaagtcct aaattatttt ttgactttga
     5281 ataattccat ctttgtcatc tactgtacta atttttgtag gataaaatct aaattctggt
     5341 tcactcaatg caccattgat agttagagaa gattcattac ctaaattacc tgagaataaa
     5401 atctgatcat cttttttaaa cttctttgaa tactcaataa ctttaggatc taccaaaatt
     5461 aaataatatg tctgatcacc attactaagc tctatagtta atttatcatt atttaaagtt
     5521 gaagaaacaa aactcccaac ccaagacgaa acagccctat tagtgtcctt tagctctttt
     5581 ttaaacttat tttcaaaatc tttaatttct aaatcattat ctgaattttt agcagtttta
     5641 tactcattag aaagagatga tattaagctt ttaaactcct gttgttcttt aggttcagtt
     5701 gtggaatttc cacaagctgt aagtaaaaaa gacagaagta aaatattaag tttattcact
     5761 attagttctt ccaaaagatc ttaatttata aatagcctaa gcagaatatg aataaaagca
     5821 atatcgacca tgatatcccc cttaaataag ctataagcta gctagctaaa tgctctcaac
     5881 ctctcaactc gcccccaaag cgtcgcactt cgtgctttgg ctttggaaac ggttgagaga
     5941 gtttcggcaa tactcaccct aagggctgtc atttaaaaaa atgacacatc ccttaagtat
     6001 tgccgattta cctgtatata tttggcttcg gagccacgta tatcggtcgt atccgcacat
     6061 agctttgctg actatgcaga caaggactca aagtcttgca cttcgcttca cgcgctccct
     6121 gtcgttacat tgcctgtatc tctgcgaggg tttccccaac gtacaactta acaagtccta
     6181 tgtaactact cccaacgctc cgcctgtgtg aagttcccct gtaagcggtt tagcctttcg
     6241 actatagcta caatacgcca gtgacactta ttcctaccac acgtactgca tgccaaccgt
     6301 aaaacggaca cgcagaagct ggcaaagctc gatctaggat acttatttga tggtttaaaa
     6361 cacgaattaa cgaagactta tcatattggg acttgatata aacactgttt attgtgaaaa
     6421 tagagttcag gtacaagcct tagtgtgaaa tcataacggt cgccaaacct tatgatcttc
     6481 gttagacgat gtgtcaccat cgaataactg cgtaccgtag aatttactgg aaaacccctc
     6541 taagttgcaa gacttagagg ggtttttgtt tataatttta aattacaaat tacttttctt
     6601 tgtttaaagc aaactttcgc ttcttaatca ctgtaatcgg cttttctagt ggagccatat
     6661 ttttctgaat ggctacctgc ttcatttcta ctggtacagc actattttca gtagactttg
     6721 gactagattc tttaggcttc gagtctgttg atttttgcag agttgcttct ttatttttaa
     6781 tttttatata aatttcatgc acgaccgcat tgtgttcttt aatatcccta tttttgctcg
     6841 ttattttttt atattgttct ttaaaaggct ttataaaatc tttgatactt gatagcagta
     6901 gagcttcatc agggtgtatg tcggctagtg tctttggtgc ttccatttgc acaaaatcaa
     6961 tgaaattcag gggaaatttt tcaataattt caggcgtcgc aaaactaata aaatcatatc
     7021 catgataaga aattacagca tatttcatat ccggcttaat ccgatgtttg atatatcccc
     7081 ttacggtcgc ttcaccctga tccagtagct tctttagata gaaatctttt atttgattct
     7141 ccgaaaatgc actatttcta taaaaagtga ttttcttatt aatttcttct cgctcttcta
     7201 tttcatcaac attctctaac ctattactaa attcaataaa ttgagcatga ttatgttgag
     7261 aaagttcaga agtccaaatt aagagttgtg caagattatc agacttaata atctcaatat
     7321 catttaaatc aagcttcttc attagtgcaa gctcttcctg cttgaactct tttctttgta
     7381 aacttgaccg cttcaacata acaacttcct taactttcac taattttaaa acattaaaac
     7441 aggtctaaat ggtcacctaa ccaaaaatct ctgaatggct atctaactgg tgtaattcta
     7501 aaacatcatc ccctacgagc ctataaatta agactaaatc gggtttaacg tggcaatctc
     7561 gacagttttt aaactcagca ccacctttta aggggtgatc taggtatttt tcaggcaatg
     7621 gactattagc gactaaacag tccagtacct cggcccattc agccgtaacc aaatctaagt
     7681 aacgtctttt aatatctcgt ttaaatgagc tagttacgat gatttttcgt ttagccattc
     7741 gcttcttccg acattgcttg gatggcttct tctactgttt cgtactcggt gtactcccca
     7801 ttttcaattt ctttaagaga ttgaagcaac tttgctgctg ctttaggggt taaagctgtt
     7861 tcccttgcat aatcaaaaga caatggaatc gttttcgttt taacaatttg atttaagaac
     7921 agtttaaatg cctgtggcat ggttaaaccg tagtcctcca aaataggagt aactttttct
     7981 tttagttctg cctctaaacg catattgaaa ttgactgcgg acatggttaa tccctccaag
     8041 aaccaataat gtaaagataa tataaggcat tatctgtact tattcattac ttttagtgta
     8101 agaatataag tatcgaagtt ccaattattg ccataacaga atttttattt tcggattccg
     8161 ctatttaggg tctattacgc aaaaatcttt atcgtttcca tatccgcact gctctttcat
     8221 cactcggacg caagttttag attcaccatt aactttgcag cttccaatat caaactgtat
     8281 accttgctgt cttaggctct cgatctgggg gatattgcct aaaggtttga tataaacact
     8341 aaataaccaa gtcatggcta gcatcaaaat ggcaaagatg cctagaacaa tggcaatgag
     8401 cttccatgaa aattgatcac tggctaattc aattgaatta tttaagcgtc cgagtgcctt
     8461 agctgtcttg ataagctctg ctgttccctc ctgtaagccc gtatttagcg actttgatac
     8521 ggtatgatgg gtttggcctg cgatgacctt ctgaatgcgt tcttcggtga tttgaagctc
     8581 tttaacggcc tgattaacta ggttctgctg ttgttctgca actgccatca gtgcatatag
     8641 ctttttttca tcaggtgtca tcggctcatc cccttataac tcatggtttt ctgttgctct
     8701 cgttctaatt tctgctgttc tttatactgc tccaaagcaa gctgtttgaa gctatcaaaa
     8761 tcagccttaa agctttcaat accctgttta atttctttgt ctgaaattgg gctgtcttta
     8821 agatctttca ggtctttttt cgggtcgcct aaatcaagtc gttcaagctc tttatgggct
     8881 gttcgtgact gttgcagggc ttcccttatt tcaggattct tcgcttggct tggtagcag
//
