LOCUS       NZ_CP048665            11857 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Acinetobacter piscicola strain YH12207_T plasmid pYH12207-6,
            complete sequence.
ACCESSION   NZ_CP048665
VERSION     NZ_CP048665.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13979601
            Assembly: GCF_015218165.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter piscicola
  ORGANISM  Acinetobacter piscicola
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 11857)
  AUTHORS   Chen,C., Sun,J., Liao,X.-P. and Liu,Y.-H.
  TITLE     Tigecycline-resistant Acinetobacter species from pigs and migratory
            birds
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11857)
  AUTHORS   Chen,C., Sun,J., Liao,X.-P. and Liu,Y.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2020) College of Veterinary Medicine, South China
            Agricultural University, Wushan, Guangzhou 510642, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 11:34:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,216
            CDSs (total)                      :: 4,108
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 108
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 67 of 164
            Pseudo Genes (incomplete)         :: 118 of 164
            Pseudo Genes (internal stop)      :: 42 of 164
            Pseudo Genes (multiple problems)  :: 51 of 164
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048665.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11857
                     /organism="Acinetobacter piscicola"
                     /mol_type="genomic DNA"
                     /strain="YH12207_T"
                     /isolation_source="feces"
                     /host="Pig"
                     /db_xref="taxon:2006115"
                     /plasmid="pYH12207-6"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="29.08 N 119.65 E"
                     /collection_date="2017"
                     /collected_by="Chong Chen"
     gene            1..951
                     /gene="repM"
                     /locus_tag="G0028_RS20775"
                     /old_locus_tag="G0028_20750"
     CDS             1..951
                     /gene="repM"
                     /locus_tag="G0028_RS20775"
                     /old_locus_tag="G0028_20750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_163172873.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKKGRSISGFS
                     FTFKQKKKDNPSIERDPNTLDLFSKMTDAQRHIFANKLSELPEMGRYSQGTESYPQFAV
                     RIAEMLQDSEKIKELAPYLKKVGYMPSNKKDIVNG"
                     /name="repM"
     gene            944..1519
                     /locus_tag="G0028_RS20780"
                     /old_locus_tag="G0028_20755"
     CDS             944..1519
                     /locus_tag="G0028_RS20780"
                     /old_locus_tag="G0028_20755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_163172875.1"
                     /translation="MAKLSLSEVSKKFHIDRSTIYRAVRNGRLSRSSDGQFDLSEVIRC
                     FGEPEQTSQQIESSTSDSDESTKKLIIHLENEVKKYQEREERLMQQIDRMQTLIELKTV
                     APATAAPQQDATACDTDMPQHATTQQDNDNKKNNELNIVENVAVPQQETTAYHTQTLQH
                     ATLQNVAVPQHKKRGLFGRVLNAVFDHD"
                     /name="plasmid replication DNA-binding protein"
     gene            1940..2284
                     /locus_tag="G0028_RS20785"
                     /old_locus_tag="G0028_20760"
     CDS             1940..2284
                     /locus_tag="G0028_RS20785"
                     /old_locus_tag="G0028_20760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146112.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA];
                     GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="WP_005146112.1"
                     /translation="MNSVLLAYVLLGFAIVSEVIGSSFLVKSEGFTKLFPSVMVFIFFS
                     TAFYLLSQVIKTIPLGIAYAIWAGVGIVLTAVVGYFVFKQTLDNPALIGIGFIVSGVII
                     INLFSQSAGH"
                     /name="SMR family transporter"
     gene            2285..2842
                     /locus_tag="G0028_RS20790"
                     /old_locus_tag="G0028_20765"
     CDS             2285..2842
                     /locus_tag="G0028_RS20790"
                     /old_locus_tag="G0028_20765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_005146114.1"
                     /translation="MKEIRQQKKQPEVIRKRILEQATILVSQKGISGVSIQNIATAVGI
                     TKGGVFHHFPNKKNLIEEMFNQIILDLDQTVEYLIKRDPTEYGKFTRAYIHSSFIHQID
                     GVVSAWSALSMTMITEPTFNKIWMEWLKNRLSQHAETDSHPDLELLRLAADGLWLQSIT
                     GVIDDEQCSKLEDDLLNRTYLT"
                     /name="TetR/AcrR family transcriptional regulator"
     gene            2965..3669
                     /locus_tag="G0028_RS20795"
                     /old_locus_tag="G0028_20770"
     CDS             2965..3669
                     /locus_tag="G0028_RS20795"
                     /old_locus_tag="G0028_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067784.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_005028420.1"
                     /translation="MNPFHGRHFQGEIILWAVRWYCKYGISYRELQEMLAERGVNVDHT
                     TIYRWVQRYAPEMEKRLRWYWRNPTDRHLWHLDETYVKVNGKWAYLYRAVDQRGHTLDF
                     YLSARRNTHSAYCFLGKILNNVKKWQIPQVINTDKAPTYGRALSRLKREGKCPPDLEHR
                     QIKYKNNVIECDHGKLKRIIRATLGFKSMKTAYATIKGIEVMRALRKGQASSFYYGQPQ
                     GEVCLINRVFGL"
                     /name="IS6 family transposase"
     gene            complement(<3723..3971)
                     /locus_tag="G0028_RS20800"
                     /old_locus_tag="G0028_20775"
                     /pseudo=""
     CDS             complement(<3723..3971)
                     /locus_tag="G0028_RS20800"
                     /old_locus_tag="G0028_20775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253717.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /name="LysR family transcriptional regulator"
     gene            complement(3999..5213)
                     /gene="floR"
                     /locus_tag="G0028_RS20805"
                     /old_locus_tag="G0028_20780"
     ARG             complement(4003..5213)
                     /name="floR"
                     /gene="floR"
                     /gene_name="chloramphenicol/florfenicol efflux MFS
                     transporter FloR"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(5365..6337)
                     /locus_tag="G0028_RS20810"
                     /old_locus_tag="G0028_20785"
                     /pseudo=""
     CDS             complement(5365..6337)
                     /locus_tag="G0028_RS20810"
                     /old_locus_tag="G0028_20785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612089.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     misc_feature    complement(5932..6048)
                     /locus_tag="G0028_RS20810"
                     /old_locus_tag="G0028_20785"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo=""
                     /db_xref="RFAM:RF01497"
     gene            complement(6520..7086)
                     /locus_tag="G0028_RS20815"
                     /old_locus_tag="G0028_20790"
     CDS             complement(6520..7086)
                     /locus_tag="G0028_RS20815"
                     /old_locus_tag="G0028_20790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006581699.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_006581699.1"
                     /translation="MPNQVGYIRVSSTEQNTERQLDGISLDKTFTEKASGGSRERPQLN
                     AMLDYLREGDTVHVHSIDRLARNTNDLNEIVNSLNDRGITIIFHKENLTFSHDVAQSAI
                     NKLMFQMLAAFAEFERSMIRERQKEGIAKAKAKGLYKGRKRKVDYVEIRKAMAEENSTF
                     RGVAEKFKVGIATVQRALKEETNNQ"
                     /name="recombinase family protein"
     gene            complement(7264..7527)
                     /locus_tag="G0028_RS20820"
                     /old_locus_tag="G0028_20795"
     CDS             complement(7264..7527)
                     /locus_tag="G0028_RS20820"
                     /old_locus_tag="G0028_20795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127801456.1"
                     /translation="MSTVDLIDEDDNESGIAAKAANVLRDRFIAAAQRGTVLYVENDNL
                     MSKTPNGVPILVKHLDGRNPDLAYRLAGRRTFKIKKRKINSN"
                     /name="hypothetical protein"
     gene            8029..8352
                     /locus_tag="G0028_RS20825"
                     /old_locus_tag="G0028_20800"
     CDS             8029..8352
                     /locus_tag="G0028_RS20825"
                     /old_locus_tag="G0028_20800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004763341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_104442387.1"
                     /translation="MNPITFQKLEKMNKHCKACVKAYALLTLLSLVTFFIYFFNRFTLE
                     ILVTDTTALFPVIKMEALALGIMHIVYCFFFKYVDKKLQIFGLDSNNLNEYIQRNQAFF
                     QKY"
                     /name="hypothetical protein"
     gene            complement(8349..8642)
                     /locus_tag="G0028_RS20830"
                     /old_locus_tag="G0028_20805"
     CDS             complement(8349..8642)
                     /locus_tag="G0028_RS20830"
                     /old_locus_tag="G0028_20805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="WP_080548207.1"
                     /translation="MQKYPDDYYLSRQEIDDLRAEAKHAAKYMKAFFDFKTEARYLFSV
                     TWSAQDGEFIGLCAEFPSLSWLDPDRDKARSGIERLVFDVLQDMSSTGEAIP"
                     /name="antitoxin HicB"
     gene            complement(8685..9677)
                     /locus_tag="G0028_RS20835"
                     /old_locus_tag="G0028_20810"
     CDS             complement(8685..9677)
                     /locus_tag="G0028_RS20835"
                     /old_locus_tag="G0028_20810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016659736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227554861.1"
                     /translation="MIYYSYVPKDFTKHVMGMMTNEYDLVAKKFILNMNTDEASRMISK
                     WIERYHLLETAQQTYRRRLNSEPVFSLLVHFTYSYLPGLSESECWEKFDKNEPAFLVQV
                     EAYLFCRTSDAFLLDEKTQKVLSKTDKQDLLKINRKIFEICPSSESFSYIGEVNPISCG
                     RYELVRLTKPKKSIKELQAKNWTNEKHVTDWTWRLTDKAYKEQLEQGKRVVLRFQSLIE
                     KNASLDEKKAYFERHFRALEGYLGYRGVRQQIGNLYHLEKRLFKDKYNQPWFDHGARTL
                     KLSYMKKLKSSIVNNSSYQEAEAHFRSVLTEDLNKKYEKWKAKSNKTEV"
                     /name="hypothetical protein"
     ARG             1941..2284
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="46.23"
                     /coverage="106.54"
     Typing          2..951
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.425"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagagatt tagttgtaaa agacaatgcc ttaatcaacg caagctataa cttagactta
       61 gtagaacaac gtttaatttt attggctatt gttgaagcaa gggaaagtgg gaaagggatt
      121 aatgcaaatg atccattaga agttcatgca gatagttata tcaatcaatt tggtgtacac
      181 cgtaatacag cttatcaagc cttaaaagat gcttgtaagg atttattcgc gcgtcaattt
      241 agctatcaag agaaaaaagc taatgggaat atccgaaatg ttatgagtcg ttgggtatct
      301 caaattgctt ataacgacaa tgaagcaact gtagacttaa tatttgcacc tgctgttgtt
      361 cccttcataa cccgactgga agaacaattt actaaatatg aattacagca agttagtagt
      421 cttagtagcg cttatgccat tcgcttatat gagcttctaa ttcagtggcg aagcactggt
      481 aaaaccccaa ccatagaact acaagaattt agaaagaagt taggcgttct tgataatgaa
      541 tatttacgga tggctcattt aaaagagcgt gttttagagc tttcaattaa acaaataaat
      601 gagcatacgg atataactgt aaaatatgaa cagcataaaa aaggtcgatc aatttcaggt
      661 ttttctttta cgtttaaaca gaagaaaaag gataatccat caatagaaag agatccgaat
      721 accttagacc ttttttcaaa gatgaccgat gctcaacggc atatttttgc aaacaaactt
      781 tcagaactcc ctgaaatggg tcgctattca caaggaactg aaagctatcc tcaatttgct
      841 gttcgtattg ctgagatgct acaagactct gaaaaaatca aagaactagc cccataccta
      901 aaaaaagtgg gatacatgcc atcaaataaa aaggacatag taaatggcta agttatcgct
      961 aagtgaagta tctaaaaaat ttcatattga taggtcaacc atttacagag ctgtacgtaa
     1021 tgggcgttta tcacgctcta gtgatggtca atttgacctt tcggaagtga ttagatgctt
     1081 tggggagcct gagcaaacat ctcaacaaat tgaatcttct acatcagata gcgatgaatc
     1141 tacaaaaaaa ctcattattc atttagaaaa tgaagttaaa aaataccaag aacgtgaaga
     1201 gcgtttaatg cagcaaatcg accgtatgca gacgcttatt gagttaaaaa ctgttgcacc
     1261 cgccacggca gcgccacaac aagatgctac ggcatgcgac accgatatgc cacagcatgc
     1321 gacaacacaa caagacaatg ataataaaaa gaataatgaa ttaaatattg tagaaaacgt
     1381 ggcagtgcca cagcaagaaa ctacggcata ccacacccaa acgctacagc atgccacgtt
     1441 gcaaaatgtg gcagtgccac aacacaaaaa acgtggatta tttggccgtg tgctaaatgc
     1501 tgtttttgat catgactgag caatctaggg agagaaatca ttccgaaact gaaagacatt
     1561 gccctgggaa ttattgtggc cccgctgctg atcccgatca tgctgattgc atcgtaccaa
     1621 gataaaaagg cactcaaaaa agagttggat gaacgccaaa aagaaaaaga ccaggcatcc
     1681 tgattattca attgagctaa tcaaaagtgt ggaacattgc tcacttatta agcgtggaac
     1741 gtgaaaagtc ataaaaaaag cccaaacagt gatgtttggg ctataaactt tgaaataagt
     1801 atttgaattt caaaataaat tataacacat ttcgtataag gtgtattatg ttaattttag
     1861 aaaatctaaa ataattattg ataatttttt tttgaaaatt taacatacct tccgattggt
     1921 attttaggta tgattactta tgaacagtgt gttactcgct tatgtattac ttggatttgc
     1981 aatagtttcc gaagtgattg gaagtagttt tttagttaaa tcggaagggt ttacaaaact
     2041 ttttccaagc gtgatggtct ttattttttt tagcacggca ttttatttat tatcacaagt
     2101 tattaaaaca atccctttag gtatcgcata cgctatatgg gctggtgttg ggattgttct
     2161 aactgctgta gttggctact ttgtttttaa acagacacta gataatcctg ctttaattgg
     2221 aattggtttt atcgtatctg gcgtaataat tattaattta ttttcgcaat cagcaggtca
     2281 ttaagtgaaa gaaattcggc aacaaaagaa gcaaccagag gttattcgaa agcgcatcct
     2341 tgaacaggca actattcttg tttctcaaaa gggtatttct ggtgtttcta ttcaaaatat
     2401 tgctacagca gtaggtataa caaaaggggg ggtatttcac catttcccga ataaaaaaaa
     2461 tttgatagaa gaaatgttca atcaaataat tcttgattta gatcaaacag ttgaatactt
     2521 gattaaacgc gatccaacag aatatggtaa attcacacga gcttatattc atagttcttt
     2581 tatccatcaa atagatgggg tggtttctgc ttggtcagcg ttatcaatga ctatgatcac
     2641 agagcctacc tttaataaaa tttggatgga atggctaaaa aatagattat ctcaacatgc
     2701 agaaacggat agtcatcctg atttagagtt acttcgttta gcagcagatg ggttgtggct
     2761 acaaagtatt accggagtca ttgatgatga gcaatgttca aaattagaag acgatctgct
     2821 taatcgaacc tatttaacat aaaatctctt attcgaaatt tgaactgtaa gccccagata
     2881 gaaaaatcca gtgcatagaa cggcactgtt gcaaataggc tgacatgata agctaaatat
     2941 cttatttatt tcgagataca gcagatgaat cccttccacg gtcggcactt tcaaggtgaa
     3001 atcattcttt gggctgttcg ttggtattgt aaatatggca tcagctatcg tgaacttcag
     3061 gaaatgctgg ccgaacgtgg agtgaatgtt gatcacacca caatttatcg ctgggttcag
     3121 cgttatgctc cagaaatgga aaaacgttta cgttggtatt ggcgtaatcc tacagatcga
     3181 catttatggc atctggatga aacttacgta aaagtgaatg gcaaatgggc atatctatac
     3241 cgtgcagttg accaacgtgg ccatactctt gatttctatc tttctgctag acgaaatacc
     3301 cattcagctt attgttttct tggaaaaatt ttaaataatg tgaagaagtg gcaaattcca
     3361 caagtgatca acacggataa agcacccaca tatggacgtg ctttatcacg gttaaaacgg
     3421 gaaggtaaat gtccaccaga ccttgagcac aggcagatta agtataaaaa taacgtgatt
     3481 gaatgtgatc atggcaagct aaagcggatc atcagggcca cattaggatt caaatctatg
     3541 aagacggctt atgccacaat taaaggtatt gaagtcatgc gtgcactacg taaaggacaa
     3601 gcatcgtcat tttattatgg tcagcctcag ggtgaagtgt gtctaatcaa cagggttttc
     3661 ggtctctaag tactttttaa agggaacatc atcgactcaa atctctattt gcaacagtgc
     3721 cccatgaagt gcctgcctgc gtctgttagc cgaacgcccc gcgcatggcg ctcaaatagc
     3781 aggacaccaa ggttatcctc cagcgctttc acacgcgcgc tgacgctcga ctagctgata
     3841 ccaagtgcct tggccgcatg ccgaaaattc agatgctcgg cgacggcgat gaactgaaca
     3901 agggaaatga gcggtatcct gccagacagg ataccgacat tcacgaggtt tcgatggtta
     3961 gtgcgccgca tcggagcggg cctgctacca gtcgtcggtt agacgactgg cgacttctcg
     4021 gtggcagccc cacggagccg aaggagcacc agccccaacg aaaccagtac cgccatcgcc
     4081 gtggcgtaac agatcacggg ccacgctgtg tcaccgttta aaagtgccac cgccaatgtc
     4141 ccgacaatgc tgactatcag gctttgaacg cagaagtaga acgcgaccgc tgatcccgcg
     4201 atgtcgtcga actctgccaa agcgccgttc gcggtaacgg acaccgtgaa gacaataccg
     4261 accgcgacaa cccacatcgg taggatgaag gtgaggaatg acggcgagcc gtaaagttcg
     4321 ccgatcccca acaggaccgc tccgcaaaca agcaacgcca tcccacgcgc cacgcatcct
     4381 gcgatgcccc atctggcgac aaaggacttc gcgaaacggg ttgtcacgat cattacaagc
     4441 gcgacagtgg cgaaggcaaa gctgaatccg atctcggaat attccgcttg gcctatgagc
     4501 acacggggag ccgtcgagaa gaagacgaag taggtgccca taccggcgct aaagccgaca
     4561 gtgtaaaccc aaaaagccgg actcgcgaag atcggcaaga cagatcggcg cgtcttgact
     4621 tgatccagag ggcgggtttc gtgccacctg aaacccgcat ttaggagtgc gagcatcgcc
     4681 agtatagcca aagtaatgaa tatcgcctgc catcccaaga actcgccgat caatactccg
     4741 gcgatagggc cgagcgcagg cacgaacgcc agcatcgaac tgaaaaggcc gtagatgacg
     4801 acaccctcag gacggttggc ataaacgtcg cgaaccgtcg cgaacgtcgc caccagcatg
     4861 gccgacgcgc ccactgcttg aagtagacgg aaagcgacaa aggccggtgc agttgaagac
     4921 caagctgctc ccagagacgc aatgacgaaa gccgttgcgc ccgcaagtag aattggccgt
     4981 cgcccgattc tgtctgagag cggaccaaaa atcacctggc ccacgccgag catcaccata
     5041 tagaggctca acgtgagttg gatcatagcg ggcgtcgtgt tcaggatgcc gggcatcgct
     5101 ggaacgacag ggagataaat atccatcgcc agtgaagcga ggatgtcgaa aggagccatc
     5161 agcagcagtg ctgccggcag cgtataggcc cacgcggggc gtgtggtggt catgacgaat
     5221 caaccctcga ttaaggaata ccgggcgacg tctgctcgtc agcaatcaga tgagactagc
     5281 cttacagagc gccgcaacaa caatactggt tgttgcggct tacttgtctg ctgacttgga
     5341 atttcccatc tgattactcc acgcttacga atatgaattg ctacatttta tccgattatc
     5401 ttttgcttcg caatgcggca tgggcgcact cagcgttcca gccccctctg ccgccctaaa
     5461 tgcttgctcc aatgcatcga gcaccatatc tgtattcatc cgtgtcgata ctttccatcc
     5521 aacaattgct cgtgagaaca catcaataat aaatgcagta taaacccagc ctgaatttgt
     5581 ttgaatatac gtaaagtcac tcacccacag ttggtcagga tgatcagcag taaaattacg
     5641 ttttaccaag tcatccgctc gtttttgatc atctcggcta cgggtggttt gtttgttctt
     5701 accacgccaa acacctttta tacctagctt ttgcattaat cgagcaacag tacagcgcgc
     5761 aataatatag ccttcacgtt tcagtttttg ccagacttta cgtacaccat accgacctga
     5821 actttccttc caaattcgtt taatctcctc agcatgatgc aagtcatgta aatctcgctt
     5881 tgctcgatgt tctggatttt cacagagatc tagagtccgg taataggttg aaggtgcgat
     5941 cggtaaaatt ctacaaatcg cctcgactcc gtacagctct ttattattat ggataaaatc
     6001 caccattatt tgtgtgggcg gtcgagctcc gcctgggcga aaaaagcggc tgctttacgt
     6061 agaatttcat tggcgcgttg cagttcttta ttttcgcgtt cgagttgttt gatacgttct
     6121 tggtctgaaa gctgctgtac tttaactgga ttttgtttat ctaaatattt ttgataccaa
     6181 acacgtagtg tttcaggagt acaacccttg cgcagtatac tgctcaggga gcaatagcgg
     6241 tgatcgcagc ccaattcgat ggataatctt tctcggattc aatcaataat tgaaccgctc
     6301 tttctctgat ttcaggggta tattttactt ttttcatcgg gacattctct cagaaagctt
     6361 ggtctccgac aaacccggta cggttcaaaa atcttccatt gatttattta tatctgttaa
     6421 accagtcgca ttatgaaatg taatataagt agaaatttca gtataaatgg tcatgatctt
     6481 ttctaaattt gaatatctaa gcgtcttcat taatcaacct tattgattat tagtttcttc
     6541 ttttaaagct ctttgtactg ttgctatccc tactttaaac ttttctgcaa cacctctaaa
     6601 tgttgaattt tcttcagcca ttgcttttcg tatttcaaca tagtcaacct tccgctttct
     6661 tcccttatat aagcccttcg ctttggcttt tgcaattcct tccttttgac gctcacggat
     6721 catggagcgt tcaaactctg caaatgcagc aagcatctga aacatgagtt tatttattgc
     6781 cgattgtgct acatcatgac tgaaggtcaa attctcttta tgaaaaataa tggttatacc
     6841 acgatcattc agactattca caatttcgtt cagatcattt gtattacgtg ccagacgatc
     6901 aatactatga acatgcaccg tatcaccttc acgcagatag tcgagcattg catttaactg
     6961 aggacgttca cggctaccac cgcttgcctt ttcagtaaag gtcttatcca ggctgattcc
     7021 atctaattga cgttcagtat tttgttcagt tgaactgact cgaatatatc cgacctgatt
     7081 aggcattctg taccgttaga gtttagataa ttaaatacat attgtaccga aattaaatat
     7141 taagtctata aatacggttt ttgagttgct ttttaagact ttattttgtt ccgttaggct
     7201 atactctaaa tttacagaac atctcttatt cgaaattcga tcgaatggga gtaaaagcct
     7261 gcctcaattc gaattaattt tacgtttttt aattttaaaa gttcgtcgcc cagcaagtcg
     7321 gtaagcaagg tcaggattcc ggccgtccaa gtgtttaacc aaaattggca caccattcgg
     7381 tgttttactc attaaattat cattttctac atacagcact gttcctcttt gcgctgcggc
     7441 aataaagcgg tctcgaagta catttgcagc tttggcagca atacctgatt cattgtcatc
     7501 ttcatcaatt agatctactg ttgacatagt gccgtactcc caattagctg atatatgaat
     7561 catattacaa gtcgggtaga caggataaag aactagagac ctttctacat ataatgctct
     7621 gatcgacaca cacaatagct tctttccaca tcaaggtagg tattatgtgt gatctgaatt
     7681 ttatatttaa tttcaataac ttataaatat taatgtttga gttttatttt tgccacataa
     7741 acaaaataat atttaaaatc aaatgcttat attttataaa aaatttaaag cttagcatca
     7801 cacatgatag ctacctaaat tagctccatt aggtagctat catgtgtgtc gatattaaaa
     7861 attatgttta atttcaatgt attaatagaa ttcaactctt tataattttg atattttttt
     7921 tacaaagcac gggatcatct aacagaaatt ttttcccttt tttaatcctg gatatttaga
     7981 atcggtagaa atttcttcac ttaaaaattt aactgcctag agagcaaaat gaatcctata
     8041 acttttcaga aactggaaaa aatgaataag cactgcaagg cttgtgttaa agcctatgca
     8101 cttttgactt tgttgagtct ggtgactttt ttcatctatt tttttaatcg tttcacatta
     8161 gaaattctcg ttacggacac gactgctcta tttccggtta tcaaaatgga agcgttggca
     8221 cttgggataa tgcacattgt ttactgcttc ttttttaaat atgtcgataa aaaacttcaa
     8281 atattcggct tagattcaaa taatctgaat gaatacatcc agcggaatca ggcatttttc
     8341 caaaaatatt aaggtatggc ttctccagtg gaactcatgt cttgtaatac atcaaagaca
     8401 agtctctcaa tgcctgagcg tgctttatca cggtctggat ctagccagga tagcgacggg
     8461 aattcggcac agagtccaat aaattccccg tcttgggcgg accatgtcac actaaataaa
     8521 taacgagctt cggtcttaaa atcgaaaaaa gctttcatat attttgccgc gtgtttagcc
     8581 tcagctctca aatcgtcaat ttcttggcgg ctgagataat aatcatcagg atatttctgc
     8641 atgagttatc accattgtta attgagattg atttaaaaaa catcctatac ctcggtttta
     8701 ttggacttcg ccttccattt ttcgtatttt ttgttgagat cttccgttaa tacactccta
     8761 aaatgagctt ctgcttcttg atatgatgaa ttatttacaa tcgaactttt cagttttttc
     8821 atatatgaaa gttttaaagt ccgggcacca tgatcaaacc agggctgatt atatttatcc
     8881 ttgaataagc gtttttctag gtgatacaaa tttccgatct gctgacgtac cccacggtag
     8941 cctaaatagc cctccaaggc ccggaaatgg cgttcaaaat aggctttctt ctcatccaga
     9001 ctggcatttt tttcaatcag gctctgaaac cgcaaaacga cccttttccc ttgttctagt
     9061 tgttccttat aggctttatc agtcaaacgc caggtccaat ctgtgacatg tttttcattg
     9121 gtccaattct tggcttgaag ctctttaatg cttttcttcg gcttggtcag acgtaccagt
     9181 tcatatctac cacagctgat tggattaacc tcaccaatgt aactaaaaga ctctgaactt
     9241 ggacagatct caaagatttt acggttaatc ttcaataaat cttgtttgtc tgttttgctc
     9301 aaaacttttt gagttttttc atccaataag aaagcatcac tggttcgaca aaacaggtaa
     9361 gcctctactt gcaccaaaaa agcaggttca tttttatcaa atttttccca gcattcactc
     9421 tcactcagtc ctggtaaata actataagtg aaatgaacca gcaagctaaa aactggctca
     9481 ctgttcaatc ttcttcgata ggtttgttgg gctgtttcca gtaaatgata acgctcaatc
     9541 cattttgaaa tcatacggct tgcttcatca gtattcatat tcaatatgaa tttcttagca
     9601 acaagatcat attcgttcgt catcattccc atgacatgtt ttgtaaaatc tttaggaaca
     9661 tagctgtaat aaatcatttt tttattactc ttaaagtgat tttaataaag tgattctata
     9721 agagatttat aaaagatatt tattaaaaat tctataagaa attcttaaag tctcaaaaat
     9781 gcaatttttg agactttaag catgattgcg aatcaccttc ggagattcta taaatagcag
     9841 ttccccaaaa tgggcttgac gaccgcaact tgatcaaata taaaactttt agggtctttg
     9901 attttttgcg tggactgcca atccatgagc caattctttt ctgtttggtt cctgggttgc
     9961 ctacccatga atcagacaca ctcccccttc ttgaggattg ccagtctttg agaagcttta
    10021 gtggtgaagc tgcgtgcgtg aggaaactct agctggcgcg gctacactag gggctggcgg
    10081 gtgtgactcc cgctttctac ctgttcactc attttgatga atgaacaccc tccactccca
    10141 aggtggaggt cagttgggcg acaaggacac gggatcacgg tgacgtggcg gtgggagtaa
    10201 cttccctcct aggctgaaag ggccaaaacc ctgtattaaa acagacagtc agaggactca
    10261 cggaatgagt aacctcgtga atagctgata accagacgct tcaagtcaaa aacaaaaata
    10321 aagacaaggg cgtaggttat acccacaggt gggcttttga gggcatcgtg aggtgtcagg
    10381 gttgcaaggc gaaagctggc gcaatcggta ctcgatttta ggtagaacag tgaatttgtc
    10441 ggaagacatt ctgttttgct gtcgagatca atggatagct caagcctgta aggtcgtcaa
    10501 catggtgctt tttaggctgt gtatgtcgat atgggtaagg cgtgaacgtg gatgattatt
    10561 aactacatat tgcattgtat aaacctgata cgacttggaa ctgcgatcgg gggagatagg
    10621 aatgtaggtg ttgtatgtag gagatcggca cgggttctgc cagttttcag gcaactgaaa
    10681 acaactgttt taatactaac ttgggaagca gttttgttta atgacgaagc cgtattgctt
    10741 aaagatttct ctttaatttg aagaggtctt tattcaatcg gaggcaactt ccgaaaacag
    10801 aactgtggga gagtgactta gtagggcttt gctgtaatgg caaaggaagc atttcaattc
    10861 gagtgaaagg cgagcgttga aatgagtaac gaaaggacac gccattaagt tggagtgtgt
    10921 aagagaattg gaagaatgtg gtttgaggat gaagtttggc aacttcatag acttggcggt
    10981 ctaaaccctc cagccacatt tgtaaaaatc gacctagaag atcagattcg agtggcatcg
    11041 gatccagaaa ttccaagaac cttgagaaaa caagtcaaat caaagtcttt gatccgactt
    11101 tttaaaaacg gttagtgggg acctcactta aaaaatcctg acgcagccga ttctgctgaa
    11161 cgaaattaaa aatctgtgtg tccctcatca cacataaata actgagatca aagtcctgag
    11221 tacgactata aactgctttc cattttgcgc tgatttgcgt tttaagccat tttctctatt
    11281 tgatataggt aatcttgata atcagtgaaa aaacgcgtct accccctatt ttttgcctct
    11341 aatcgttata tcagagcgag tgtctacgag cgaacatcaa aaagtcgtgc ctagactccc
    11401 tgaaaaacag ctttttaggc tgtgaagtca aaaaaggcgt aaatcatatg tagaaaattg
    11461 agcctagacc gagcgaagcg agcaaaaaag tactatgagc gaagcgaatt ccgagttgct
    11521 tttgcttttt ctaaaactca cgaaaatatt aaccaaaaaa ttgcccctcg aatcgagcga
    11581 aagcgagatt caatagagtt tgagcgaagc gaaaaccaag ggcaattttt cattcccttg
    11641 gcttttaatt attttaaagt ttttaaatgc ttttaaatgg cttgaaagcc ttatgaataa
    11701 agggttttag ctcttatatg tccacgttta ccttgcaata tgtccacgtt taccttgcaa
    11761 tatgtccacg tttaccttgc aatatgtcca cgtttacctt gcattagtac acaaataata
    11821 ttaacgtgta cttatacaca ataaaaaaat agtggct
//
