LOCUS       NZ_CP048664            12678 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Acinetobacter piscicola strain YH12207_T plasmid pYH12207-5,
            complete sequence.
ACCESSION   NZ_CP048664
VERSION     NZ_CP048664.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13979601
            Assembly: GCF_015218165.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter piscicola
  ORGANISM  Acinetobacter piscicola
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 12678)
  AUTHORS   Chen,C., Sun,J., Liao,X.-P. and Liu,Y.-H.
  TITLE     Tigecycline-resistant Acinetobacter species from pigs and migratory
            birds
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12678)
  AUTHORS   Chen,C., Sun,J., Liao,X.-P. and Liu,Y.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2020) College of Veterinary Medicine, South China
            Agricultural University, Wushan, Guangzhou 510642, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 11:34:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,216
            CDSs (total)                      :: 4,108
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 108
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 67 of 164
            Pseudo Genes (incomplete)         :: 118 of 164
            Pseudo Genes (internal stop)      :: 42 of 164
            Pseudo Genes (multiple problems)  :: 51 of 164
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048664.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12678
                     /organism="Acinetobacter piscicola"
                     /mol_type="genomic DNA"
                     /strain="YH12207_T"
                     /isolation_source="feces"
                     /host="Pig"
                     /db_xref="taxon:2006115"
                     /plasmid="pYH12207-5"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="29.08 N 119.65 E"
                     /collection_date="2017"
                     /collected_by="Chong Chen"
     gene            complement(<1..729)
                     /locus_tag="G0028_RS20700"
                     /old_locus_tag="G0028_20680"
                     /pseudo=""
     CDS             complement(<1..729)
                     /locus_tag="G0028_RS20700"
                     /old_locus_tag="G0028_20680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611903.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY-like element ISAjo2 family transposase"
                     /name="ISNCY-like element ISAjo2 family transposase"
     gene            complement(906..1430)
                     /locus_tag="G0028_RS20705"
                     /old_locus_tag="G0028_20685"
     CDS             complement(906..1430)
                     /locus_tag="G0028_RS20705"
                     /old_locus_tag="G0028_20685"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_180047728.1"
                     /translation="MKQRLVFIAEGPTEKALLHHLGIEGKKETINLAQTESRKFERYFG
                     NLPKPNMSEIYLLIDTDLILNFPHCLKNIEENILLLTKYGYKIFVLQQHRDLEDELAYA
                     CGFKNINKLLERVNCTTKNELKGKVIKNPSCVTSINSFEKSKLWNRNLIEELKHCVTKE
                     MKKSFDDLKAK"
                     /name="hypothetical protein"
     gene            complement(1443..2693)
                     /locus_tag="G0028_RS20710"
                     /old_locus_tag="G0028_20690"
     CDS             complement(1443..2693)
                     /locus_tag="G0028_RS20710"
                     /old_locus_tag="G0028_20690"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_180047730.1"
                     /translation="MIFTKLHLKNWYSFKDCELDLTYPKKIKNNTIPYEFLENFPNIRV
                     KRLVLISGANASGKTSFTKILYAIKDFISEGSISGFLEDGLRSHEEKISFSVEFIAAKP
                     IYILDLARKPQEELRDYITEVNTRYDHLHSLDVEFDLTKKELKEHSWKYTYKCIPINKQ
                     DSINSLRKKLETVDENSIGQNVIFSTNIDNVDNKNHRDFDEYYFASAKNFLSCDIENKF
                     FKDINLDKNILERCLKTFDPTIKNVAYASEQNSDTPKGFFINFCNNKSLQMSLIGTVIE
                     DRHLLSQGTYEAIKLAKFLSRVINLPQNRSFTYVLDENMSHVQCEIEKNMLNLIIQKIS
                     HTSQLFYTTHNYEVLEMNYPIHSYLFIKRDIDGNSEFIKAEDHFKKNDRSIVNYVRNDV
                     LGTIPDTNLLDEILFED"
                     /name="ATP-binding protein"
     gene            2890..3069
                     /locus_tag="G0028_RS20715"
                     /old_locus_tag="G0028_20695"
     CDS             2890..3069
                     /locus_tag="G0028_RS20715"
                     /old_locus_tag="G0028_20695"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_180047732.1"
                     /translation="MKLNETTALAKEINMIKDSNTLVHTKNTTFNTSFKQASMLENIQM
                     FIVGMVSSAWGAFA"
                     /name="hypothetical protein"
     gene            complement(3171..3566)
                     /locus_tag="G0028_RS20720"
                     /old_locus_tag="G0028_20700"
     CDS             complement(3171..3566)
                     /locus_tag="G0028_RS20720"
                     /old_locus_tag="G0028_20700"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_180047734.1"
                     /translation="MIFFKKIKEKIILNKKNEQAFYEIALTEFSTGEKRPGLWAMALAK
                     SEGVIEKANALYIGLLAEEIKNDEYLELNKIENEQRIEKLIKSEREKEFNRVKKIVDNL
                     KKEQEKERKKLIDPRFKEEQPFLKKEH"
                     /name="hypothetical protein"
     gene            complement(4132..4473)
                     /locus_tag="G0028_RS20725"
                     /old_locus_tag="G0028_20705"
     CDS             complement(4132..4473)
                     /locus_tag="G0028_RS20725"
                     /old_locus_tag="G0028_20705"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_180047736.1"
                     /translation="MEDQLNLLNDLAIEIFTLLDGKEHPYRYEYKFNPDEGWAQFGLYF
                     TKNSEMTSVSLNEISFEHLIPLCEKLHQVMNAHTGGDWRKFILDINEKKEVKTNFIYEI
                     QSCMDEFND"
                     /name="hypothetical protein"
     gene            4672..5151
                     /locus_tag="G0028_RS20730"
                     /old_locus_tag="G0028_20710"
     CDS             4672..5151
                     /locus_tag="G0028_RS20730"
                     /old_locus_tag="G0028_20710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004940252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_180047738.1"
                     /translation="MNSIDPHQKINRIKDESIAPQQLGTLTYRLSLTQKFVTWYNTHYE
                     SNWFCLNCEKDDETVMDPNTVKDHFENQVIQCQRCDINDSQGGLPSGKRRDREAWKSAQ
                     SIVSHSFDPEIIKATKKRSWERFERIQSGETTIEDEEAVLLMQWRALNNLLKSQD"
                     /name="hypothetical protein"
     gene            complement(5135..6418)
                     /locus_tag="G0028_RS20735"
                     /old_locus_tag="G0028_20715"
     CDS             complement(5135..6418)
                     /locus_tag="G0028_RS20735"
                     /old_locus_tag="G0028_20715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005175445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_180047740.1"
                     /translation="MFYCNLEHTSKKAVKTRSGKVKNAATAKNRFHYITRTSHFKNYKE
                     SASEQIEFVRSGNLPNFAEGNPAEFWDAADIYERSNGRTCSSLVVALPKELNQAQRIEL
                     AEAFITEFADRYSYPFTCAIHNHVGSLGGQEQPHLHFMYSERHVDGIDRTAEQFFKRYN
                     PKDPQKGGAQKLTADVLGMGKAQLQLYRQKTEELINDSLRRYAPIKMVEIRGIQVEVPS
                     LVSCLSNRDYNQKYGTQLQDVPVMNKAVRFAREKEPELMAEREKMTTEINRLRAENHYE
                     LYRSFYEAELKNRKEIEALSKQAETPRTRQRWYAQEKLLFLQQKMLQALLKDHQMTEPF
                     RLIQHDLQTYIDLLNTESFDRLEKLEYLSDLGQQLKNSVIQIAAIDEELAFMKRQVIER
                     VPLQMREKAVTPEKIKNHDPDNDFSPGF"
                     /name="MobA/MobL family protein"
     gene            6640..7395
                     /locus_tag="G0028_RS20740"
                     /old_locus_tag="G0028_20720"
     CDS             6640..7395
                     /locus_tag="G0028_RS20740"
                     /old_locus_tag="G0028_20720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004775063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_180047742.1"
                     /translation="MAARGRPAKILTPNDLTTLQQFLEKLPFLKKKQQQALTLLQQSNG
                     RFNDQQLSLFKTVDREKNQFLTRQALIEQIKLKDKNQQPLLANEIEILTLLNQAMDQDN
                     FFRLDRALESYQKIEKAALDNRIRLENEHKREILQKSHKELTEAQKKRNAENQLKYAIG
                     GVVQSLYKDLNLQIQHSDIPLLRDALLQSIIWEKTIKETTIYKEIQNNTEDIKEETKLF
                     QRMLEDLPTYKINGKDFHKVLIQKHSKKP"
                     /name="hypothetical protein"
     gene            8137..9090
                     /gene="repM"
                     /locus_tag="G0028_RS20745"
                     /old_locus_tag="G0028_20725"
     CDS             8137..9090
                     /gene="repM"
                     /locus_tag="G0028_RS20745"
                     /old_locus_tag="G0028_20725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_180047744.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGRGINTNDP
                     LEVHAESYINQFQVARQTAYQALKDACKDLFARQFSYQEINKRGNIENVLSRWVSEIRY
                     IDDEATVKLIFAPAIVPLITRLEEQFTKYELQQISNLSSAYAVRLYELLIAWRSTGQTP
                     IIELAEFRKKIGVLDDEYTRMGNFKDRVLNLAVAQVNEHTDIKVKCEQHKRGRNIAGFS
                     FSFKQKKDAALNTINKNAKTLDVSLKLTDAQRHMFAHKMSELPEMSKYSQGTESYPQFA
                     VRIAEMLQDSERLKELLPYLIKVGYMPLDKKDTVNG"
                     /name="repM"
     gene            9083..9637
                     /locus_tag="G0028_RS20750"
                     /old_locus_tag="G0028_20730"
     CDS             9083..9637
                     /locus_tag="G0028_RS20750"
                     /old_locus_tag="G0028_20730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_180047746.1"
                     /translation="MAKLSLSEVSKRFKISRSTLYRAIKEGRLSRGADGLFDIAEVVRC
                     FGEPTKIQDQSNSTSPPQNDADLRQLVDFMKKEIEGYKDREKRYLDQIDRFQLLLGHKE
                     DEEELSHDTPITPHNDTPCDTTNEMVESFENKRFNNKNKSHEIPMEHTNDTLRDISVTH
                     HATRKKRGLFGRVLNAVFNED"
                     /name="plasmid replication DNA-binding protein"
     gene            9659..9802
                     /locus_tag="G0028_RS20755"
     CDS             9659..9802
                     /locus_tag="G0028_RS20755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_180047748.1"
                     /translation="MKILKEILQAVAFLLIGIPIMIMASISDQRAFKKEREQRGKAQEQ
                     NR"
                     /name="hypothetical protein"
     gene            10092..10691
                     /locus_tag="G0028_RS20760"
                     /old_locus_tag="G0028_20735"
     CDS             10092..10691
                     /locus_tag="G0028_RS20760"
                     /old_locus_tag="G0028_20735"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_180047750.1"
                     /translation="MDIYQDCSCSGNNENCFKCYGTGRIIDENKTSTPKSIKVLSTNHT
                     RFKKESYKKEVFILKKNIDGKIYKEFLVKCKICAKLIEKIEIKNHIIKHNSMNHIESSQ
                     ETNLVKKQKITLRTLILCPICNVRVKGVKYEKHMFKVHGKNIDLSNFKINVSSGSFGIY
                     DDVIEKKINNDATKLYAHNFRENGKFGSHPEHDNYD"
                     /name="hypothetical protein"
     gene            complement(10770..>11372)
                     /locus_tag="G0028_RS20765"
                     /old_locus_tag="G0028_20740"
                     /pseudo=""
     CDS             complement(10770..>11372)
                     /locus_tag="G0028_RS20765"
                     /old_locus_tag="G0028_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611903.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /name="ISNCY family transposase"
     gene            complement(11409..12628)
                     /locus_tag="G0028_RS20770"
                     /old_locus_tag="G0028_20745"
     CDS             complement(join(11409..12352,12352..12628))
                     /locus_tag="G0028_RS20770"
                     /old_locus_tag="G0028_20745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611467.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_104915068.1"
                     /translation="MTKLKYTPEIRERAVQLLIESEKDYPSNWAAITAIAPKIGCTPET
                     LRVWYLKHMDQLNPAKVQQISDQEKMKQMEREIKELKRANEILRKAAGFFRPGGARPPT
                     QIMVDFIHNNKDRYGVEAICRILPIAASTYYRALDLVDNPEHRAKRALHDLHHAEQIKR
                     IWKESLGRYGVRKVWQKLKREGYVIARCTVARLMQKLDIQGVWRGKNKQTTRSRDDQKR
                     ADDLVKRNFSADRPDQLWVSDFTYIQTNSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMV
                     LDALEQALHDRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAET
                     VNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKERVHSALGYVSPFEFEAMYYDKI
                     NPLGQVA"
                     /name="IS3 family transposase"
     misc_feature    complement(12237..12353)
                     /locus_tag="G0028_RS20770"
                     /old_locus_tag="G0028_20745"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     Typing          complement(5139..6418)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.138"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11413..12311)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttggcctgt ggtgaattag cgaagataat atcgatattc agggtgctga gtacgcgccc
       61 aaactgggta atcttggtgt ctttcttact gctttgattc accctgaaga ctgaatgttt
      121 atcgctgtag aaggctaaag gcttaccatg ctgctcaaca tacaaacgtg ttgaaatcat
      181 atagtcaaag gttgattccg actcacagaa gcgtaaatgc tgtaatttgc ctgtggcatc
      241 atcgataaat accagcagac agcatttagg ggctcgacct tcaaaccagt catggtgtga
      301 gccatcgatt tggatcagtt caccataaca gtcccggtta taacgaggct gatacgggcg
      361 tttcagacgc ttggcgcgag gaatccataa atcggctgca atcatccagg aacgcagtgt
      421 ttccactgaa atatcaaagc catgaacggt ggtgagcttt tcatgcgcta aagtgggtcc
      481 gaaaccatgc agttgatcag aaacaatatt gaggcacttg agtctgagct cttcaggaag
      541 cctggaattg ctgatttgac cacgaccggc atgggctaat gcagctgggc cttgagcttt
      601 gtatttttgc aataaacgtc tgatctgacg ctctgaaata tgaagtagct gagcagcttg
      661 ggactgggtt atgcgttgat cacagatttc ctgcaagacc gacaatcgtt taagttcttt
      721 atccgacata gacaccaaca tatcaaaccg tccgctaaga aaattgcgaa agtgcatatt
      781 ctaaaagcgg acatttttac tttggagaaa ccggacattt ctactttggg cttacattgt
      841 aacttcgcat aagatgtatt atgttaattg tagatgaact ataaaatatc tatagaagta
      901 gctattcact tagccttaag atcatcaaat gatttcttca tttccttggt gacacagtgc
      961 ttcaattctt ctattaaatt tctattccac aacttacttt tttcgaatga attaatagat
     1021 gttacacagc taggattttt aataactttg cctttaagtt catttttagt agtacagttg
     1081 accctctcta ataatttatt aatattttta aatccacacg cataagccaa ttcatcttcc
     1141 aaatctctat gttgttgtaa aacaaaaatt ttatatccat attttgttaa caacaaaata
     1201 ttttcttcaa tatttttcaa acaatgaggg aaatttaaaa ttaaatcggt atctataaga
     1261 agatatattt cagacatgtt cggcttaggt aagttaccaa agtatctttc aaacttccta
     1321 ctttcggttt gagctaaatt aatagtttct ttttttcctt ctatccctaa gtgatgtaat
     1381 aatgcttttt cagtaggacc ttctgcaata aatactagac gctgtttcat cacaattaca
     1441 tctcaatctt caaataaaat ttcatctaat aaattagtgt cagggatagt tccaagaaca
     1501 tcatttctaa cataatttac aatagatcta tcattctttt tgaagtgatc ctcagctttt
     1561 ataaattcag agttcccatc tatatctcgt tttataaata aataactatg aataggatag
     1621 ttcatctcta aaacttcata attatgcgtg gtataaaaca attgagatgt atgactaatc
     1681 ttttgtatta tcagatttag catgtttttt tcaatttcac attggacatg agacatattt
     1741 tcatctagta cataagtaaa acttctattt tgaggaagat tgatgactct tgataaaaat
     1801 ttagctagtt taattgcttc atatgtaccc tgagaaagta agtgtctatc ttcgatgaca
     1861 gtaccgataa gactcatctg taaactcttg ttattacaaa aatttataaa gaaccctttt
     1921 ggagtatcgc tattttgctc agatgcatat gcaacatttt taattgttgg gtcgaatgtt
     1981 ttaaggcacc tctctaaaat atttttatct aaatttatat ctttaaaaaa cttattttca
     2041 atatcgcaag acaaaaagtt tttagcagat gcaaaataat attcatcaaa atcacgatgg
     2101 tttttattgt ctacattatc gatattagta gaaaaaataa cattttgacc aatactgttt
     2161 tcatcaactg tttctaattt tttccttaaa gaattaatac tatcctgctt attaatagga
     2221 atacatttgt aagtatattt ccaagagtgt tcctttaact cttttttagt taaatcgaat
     2281 tcgacatcca gactatgcag gtgatcatat cgtgtattaa cctctgtaat atagtctcta
     2341 agttcttctt gaggcttacg ggccaaatct aaaatataaa taggtttagc cgcaataaat
     2401 tcgacactaa aggatatttt ttcctcatgg cttcttaagc catcttccag aaaaccagat
     2461 attgatcctt cagaaataaa gtctttaata gcataaagaa ttttagtaaa agatgtttta
     2521 ccacttgcgt tagcaccaga aatcaatact aaacgcttaa ctcgaatatt agggaaattc
     2581 tctaaaaact cataaggtat tgtattattt ttaatttttt taggatatgt aagatccaat
     2641 tcacaatctt taaaactata ccaattctta agatgtaact ttgtaaaaat cataacattt
     2701 tccaattttt ggaagtaaag caaaataact actatacatt aaaattaaaa atttaaatat
     2761 tccttgctat acttaaatga tgtgcttagg caagtgcatt cactgggcga tgttcttttg
     2821 atcggaataa tcatcttact aacagtagtt ggtttctatc tcgcttctag cttgggagaa
     2881 atcaaattca tgaaacttaa tgaaactaca gctttagcaa aggagataaa tatgattaaa
     2941 gactcaaaca ctctagtcca tacaaaaaat actactttta acacttcttt taagcaggca
     3001 agcatgcttg aaaatataca aatgtttata gtaggtatgg ttagctccgc atggggagca
     3061 tttgcctaag catttcaaat taaaaattag ataattcatt taacataaaa cctcttatga
     3121 gaaattagac tataagtcca aagtcgaaat gtccagtttc tccaaagatt tcaatgctct
     3181 tttttaagga aaggctgttc ctccttaaat cttggatcta taagcttttt cctctctttc
     3241 tcttgctctt ttttcaaatt atcaactatt ttttttactc gattaaattc tttttctctt
     3301 tcagacttta ttagcttttc tatccgttgc tcattttcta ttttatttaa ttctaaatat
     3361 tcatcgtttt ttatttcctc cgctaataaa ccaatatata acgcattagc tttttcgatt
     3421 actccttctg actttgctag tgccattgcc caaagacctg gccgtttttc tccagttgaa
     3481 aattctgtta atgctatttc ataaaaagct tgttcatttt ttttattaag tataattttt
     3541 tctttaattt ttttaaaaaa aatcatcaga ttcattccct atataatagt cattcaatga
     3601 agttatgatc tcaataattt aacttctttt acaagcttat ttttaagaaa tggcaataaa
     3661 aaaccccgac atcctgtcgg ggtttttcgt atttgcgtct tagggtcaac tcctgtcatc
     3721 cctcacgatc ctggtgtttt gcttattatg attgttttat gctttggagc atcctgctcg
     3781 ctatgggttc atgatagaaa attagaggga atctgcctat tcgccaatac cttaaaatgt
     3841 tgtacgttgt ggcttacaaa gcttaaaaat tatccttaga tttatccgca cattttgtgg
     3901 atattttaac cactataagt cttgattgaa ttcagaaaaa tttaaaattg gttgtttttt
     3961 aacctatttt ataggcaaaa agtgacaatt ccgtttaaaa aagcgccgac atgtaacgag
     4021 gtcggctttt ggatcgtttt ttgagttttt aaattaggct aattgattga tattacaggg
     4081 ttattacaaa acgatttcgc ataacgccca ttatgttaaa tggacataat ttcagtcatt
     4141 aaattcgtcc atacatgatt gaatttcgta aataaaattg gttttaacct cttttttttc
     4201 attaatgtcc aaaataaatt tccgccagtc tcctcctgta tgagcattca taacttgatg
     4261 taatttttca cacaaaggaa ttaaatgttc aaaacttatt tcgtttaagc ttactgatgt
     4321 catttcgcta ttttttgtga aatataaacc aaactgtgcc caaccctcat caggattgaa
     4381 cttatattca taacgataag ggtgttcctt cccatccaat aatgtgaata tttcaatggc
     4441 taagtcattg agtaaattta attgatcttc caaaatttgc acctatcatt aaattattta
     4501 ttttaaatat ttcctaaaat taacataata cgccttatgc gaagtgcata aagaatggga
     4561 gctaaaagct cccatttcta attttactca taactaaaaa tttcaataaa aaatatacgc
     4621 tcaaatttaa tttagactat aagaaatcaa tacgaaaata aggctaaagc catgaatagt
     4681 atcgatcctc accaaaaaat aaatcgaata aaagatgaaa gtattgcccc acaacaatta
     4741 ggcacactga cttatcgact atctttgact caaaagtttg tcacttggta caacacccac
     4801 tacgaaagca actggttttg cctaaactgt gaaaaagatg atgaaacagt gatggatcca
     4861 aacactgtaa aagatcattt tgaaaatcaa gtcatccaat gccaaaggtg tgacatcaat
     4921 gactcacaag gtggtttacc atcaggaaaa agacgagatc gagaagcatg gaaatccgca
     4981 caatccattg tttcacatag ctttgatcct gaaatcataa aggctactaa aaaaagatcc
     5041 tgggaacgct ttgaacggat ccagtccgga gaaaccacaa ttgaagatga agaagcagta
     5101 ctactcatgc agtggcgtgc cttaaataat ctgcttaaaa gccaggacta aaatcattat
     5161 ccggatcatg attcttgatc ttttcaggag tgactgcctt ttccctcatc tgcaaaggca
     5221 cacgttcgat gacttgccgc ttcatgaatg ccaattcttc atcaatagcg gctatctgaa
     5281 taactgaatt ctttaactgt tggcccagat cacttaagta ttctaatttc tcaagtcgat
     5341 caaaggattc ggtattcaat aggtctatat acgtctgtaa atcatgctga atcaacctaa
     5401 aaggttctgt catttgatga tctttcaata aagcttgcaa cattttttgt tgtagaaaca
     5461 ataatttttc ttgagcatac caacgctgcc gtgtccgtgg tgtttctgct tgcttgctca
     5521 acgcttcaat ctcttttcga ttttttaatt cagcctcata aaatgaacga tacaactcat
     5581 aatgattttc agcacgtaac cgattaattt cagtagtcat cttttcccgt tcggccatta
     5641 attccggctc tttctcacgc gcaaaacgca ccgccttatt catcacgggg acatcttgca
     5701 actgcgtccc atacttttga ttgtaatccc gattcgacaa acaactgacc aaactaggca
     5761 cttccacctg aataccacgg atctccacca ttttgatggg ggcataacgc ctcaaactgt
     5821 cattaatcaa ttcttcagtc ttctgcctat acaactgcag ctgcgctttc cccatcccca
     5881 atacatcagc cgtcagcttt tgcgcgccac ctttctgcgg atccttcggg ttataacgct
     5941 taaaaaactg ctcagctgtc cgatcgatcc catccacatg ccgttcacta tacataaaat
     6001 gcaaatgcgg ttgctcttgc ccacccaacg atccaacatg attatgaatg gcacacgtaa
     6061 agggatagct atagcggtct gcaaactcag taataaatgc ctcagccaat tcaatccgct
     6121 gcgcctggtt caactctttc ggtaaagcca ccaccaaact ggaacaggta cggccattac
     6181 tgcgttcata aatatctgca gcatcccaaa actcagccgg atttccttca gcaaaatttg
     6241 gcagattgcc ggatctcaca aactcaatct gctccgaagc cgattcctta taatttttaa
     6301 agtgcgaagt ccgggtaatg tagtgaaacc ggttcttggc cgtggcagca ttttttactt
     6361 tccccgatcg ggttttcacg gcctttttgc ttgtgtgttc cagattgcaa tagaacataa
     6421 tcccaccttc aaattcacgg agcctcgcag agcctgcgga gcacgaggag aagtgttttt
     6481 aattcgatat aattgcgtag taattatatc gaattaaaaa cacggaaacg agcttaagaa
     6541 ttaaataaaa tgttcccaca ttttatttaa ttcttcccgt gccggcctat gctaaactga
     6601 aaacacaaaa acctcaccca ttttatgctg gagaatgcga tggcggcccg tggaagacct
     6661 gcgaaaatac tcaccccaaa tgacctcacg acattgcagc aattcctgga gaaacttccc
     6721 ttcctcaaga aaaaacaaca acaggctttg accctacttc aacaatccaa tggcagattt
     6781 aatgaccaac aactcagcct gttcaaaaca gtcgaccgag agaaaaacca gtttctaaca
     6841 agacaggctc tcattgaaca aatcaaactg aaggacaaaa accaacagcc cctcttggcc
     6901 aacgaaattg aaattctgac cttactcaat caagcaatgg atcaggataa tttcttccgc
     6961 ctagatcgtg ccctagagtc ttatcagaaa attgaaaaag cagccctaga caatcgaatc
     7021 cggctggaga atgaacacaa acgtgaaatt ttgcagaaaa gtcacaaaga actgacagaa
     7081 gcccagaaaa aacgcaatgc agaaaaccaa ctcaaatacg cgataggagg tgtagttcaa
     7141 tcgctttata aagacctcaa tcttcagatc cagcacagtg atattcccct actcagagat
     7201 gccctcctac agagcattat ttgggaaaaa accattaaag aaaccacaat ctataaagag
     7261 atccaaaaca acaccgaaga tatcaaggaa gaaacaaaat tattccagcg tatgcttgaa
     7321 gatttgccaa cctataaaat caatgggaaa gattttcaca aggtactcat tcaaaagcac
     7381 agtaaaaagc cataaaatgc gattacagcc tgtttttggc tcaatcctat aaccctacat
     7441 aaaaaatgta aacagggcta aaaatgcgaa ataagccctt ttacgcttca accctaaatg
     7501 aatccttaat ttataaatta agaaatgccc ttaaaactca aattaaatac ctaaaaggta
     7561 tctatagagc gagtgtctac gagcgacaca atgaaaagtc gcgtctagac cctctgaaaa
     7621 accgttttta aggctgtata gcctaaaaaa gcataaatca catgctaaac ctggcttcta
     7681 gatcgagcat agcgagcaaa aagctttatg agcgaagcga attcattatg ctcttgcctt
     7741 tgcttttgct ctttttaact tcacgtaaac atgaaccaaa aaattgcccc aagaatcgag
     7801 cgagagcgag atttaataga gtttgagcgt agcgaaaaca tagggcaatt tttaatgttt
     7861 tgtgttttta attgctttta aaatattttt aaaagctttt agactatctg aaagctttat
     7921 atagcaatgc tttcaaaacc aatatgacta cgtttaccta ccaatatgac tacgtttacc
     7981 taccaatatg actacgttta cctaccaata tgactacgtt tacctaccaa taatctacag
     8041 tatatttttt gtagatttaa ctacatttac cttgcataaa tctacataat attttatata
     8101 gacttatatc ggtaaataat aaagtagata aatagcatgc gagatcttgt tgttaaggat
     8161 aatgctctaa ttaatgctag ctataacctc gatttagttg aacagcgttt aattctcttg
     8221 gctattgttg aagcaagaga aagtggtaga ggtatcaata ctaatgaccc actagaagta
     8281 catgcagaaa gttatatcaa ccaattccaa gtagcgagac aaacagctta tcaagcctta
     8341 aaggatgcct gtaaagattt atttgctcgg caatttagct atcaagaaat aaataaaaga
     8401 ggaaatattg agaatgtttt aagtcgctgg gtcagtgaaa ttagatatat tgatgatgag
     8461 gcgactgtaa aattaatttt tgctcctgct attgttcctt tgattacacg tttagaagaa
     8521 caatttacaa aatatgaatt acaacaaatt agcaatctta gtagtgctta tgcagttcga
     8581 ttatatgagt tattaattgc atggcgtagt acagggcaaa caccaattat tgaattagct
     8641 gaatttagaa aaaaaattgg tgtattagat gatgaataca ctcgtatggg aaactttaag
     8701 gatcgtgtcc ttaacttagc agttgctcaa gtcaatgagc atacagatat taaagttaaa
     8761 tgtgagcaac acaaacgagg aagaaacatt gctggttttt cattttcctt caaacagaaa
     8821 aaagatgctg cattaaatac cataaataaa aatgctaaga ctttagatgt atctctaaaa
     8881 cttaccgatg ctcaacgcca tatgtttgcg cataaaatgt ctgaattacc tgaaatgagt
     8941 aaatattctc aaggaactga aagctatcct cagtttgcag ttcgtatcgc agaaatgctc
     9001 caggattctg aacgtctcaa agagttgtta ccttatctaa taaaagttgg gtatatgcca
     9061 ttagataaaa aggacacagt aaatggctaa attatcactc agtgaagtat ctaaacgttt
     9121 taagataagc cgttctactt tatacagagc tataaaggag gggcgtttat ctagaggtgc
     9181 tgatggtctt tttgatattg ctgaagttgt acgttgcttt ggcgaaccaa caaaaataca
     9241 ggatcagtca aactcaactt ctccccctca aaatgatgca gatttacgcc aattagttga
     9301 tttcatgaaa aaggaaattg aaggatataa ggatcgtgaa aaacgctatt tagatcaaat
     9361 tgaccgcttc caattactat taggacataa agaagatgaa gaagaattat ctcatgatac
     9421 acctataaca ccccacaatg acacaccatg cgacacaaca aatgaaatgg ttgaatcatt
     9481 tgaaaataaa agatttaata ataaaaataa gtctcatgag ataccaatgg aacatactaa
     9541 tgacacacta cgagacatat cagtaacaca ccatgcaaca agaaaaaaac gtggcttatt
     9601 tggacgagtc ctgaatgccg tatttaatga ggattaaact cttagaggag ttttgagcat
     9661 gaaaatactg aaagagatcc tacaagcagt tgcctttttg ctgattggca ttccgatcat
     9721 gatcatggct agtatttcag atcaacgagc ctttaaaaag gagcgagaac aacgtggaaa
     9781 agcccaagag caaaacagat gattgttcag taaagtttac caatgaaaat caaaattgag
     9841 tataaaacag gcatattgtg tgaataatcc ataaaaaaca ctgatttaaa tcaagttatg
     9901 tattgaattt cgcataagtt gtattatgct aattttagtt tttctaatag agatagagga
     9961 atcttttaca agttggctgt ctagaatctc ccaagtgaaa gtctcctgtt atttcagcac
    10021 acataatttg tcttacttga gaatcatagg taaaatattc aataacttta aaataaaaat
    10081 ttgggtaaat tatggatatt tatcaagatt gttcttgtag tggaaataat gaaaattgtt
    10141 tcaaatgtta tggaactgga agaattattg atgaaaataa aacttctaca ccaaaatcaa
    10201 taaaagttct ttcaactaat catacaagat ttaaaaagga gagttataaa aaagaagttt
    10261 ttattttaaa aaaaaatata gatggaaaaa tttataaaga atttttggtt aagtgtaaaa
    10321 tatgtgcgaa attgattgaa aagatagaaa taaaaaatca tatcattaaa cataattcta
    10381 tgaatcatat agagtcttca caagaaacta atctcgtaaa aaaacaaaaa ataactctaa
    10441 gaacattaat actctgcccc atatgtaatg ttcgtgtaaa aggagttaaa tatgaaaaac
    10501 atatgtttaa ggtacatgga aaaaatatcg acttatctaa ttttaaaata aatgttagtt
    10561 caggtagttt tggtatatat gatgatgtaa tcgaaaaaaa aataaataat gatgctacta
    10621 aactctatgc acataatttt agagagaatg gaaaatttgg ttcccatcca gaacatgata
    10681 actatgatta gcctatttta aattaacaca atacattgta tgttattcca aagtagaaat
    10741 gtcctaccta ataaccaaat agaaatgtcc tatatggaca tttccaagtc aagcacagga
    10801 tttagatttc gttcttgaat tgctgttttc tgtgcacgtc tgcttggcat cttttgagaa
    10861 cgattgcgtt tattttgttg ttctagttct tcatgctgct gttgaatatg ggctaataca
    10921 gaacccaaac gtttattttc aacaatctct cgctggttga gctgacttaa tttatcgaag
    10981 atgctgtaat tgatttttct tccctgatat tcaatcgcga ctgtactatc cgggtattcc
    11041 agaaactcaa gatacttgcc aatcaatctt tggttttctt ccgtgttttc caggagatat
    11101 acgcatttat cataagtgat cgtcaggcta ttcgtgactc tgcggggttc acgccaggta
    11161 aaaatatcat ctaattcttc ggctgtttca gtgacagtcc ggtgtagatc cttgggatta
    11221 aaggccatct tggcaaactt ctgattgaac tgctcaatga agcagggtag ccaggcattg
    11281 gcttcggcaa tcgaactgat gccttccaga cgcatttctt tgatcagacg gtcctgaagc
    11341 gttctattgg ctcgttctac acggcctttt gaaccgtacc aggtttgtcg gagacttttt
    11401 tatttaagtt aggccacctg acctaacgga ttaatcttat catagtacat tgcttcaaac
    11461 tcaaaaggcg atacataacc cagtgcgctg tgtacacgtt ctttattgaa ccaatcgacc
    11521 cagtttagtg tcgcaagttg tacatctgct aaaccttgcc aatctgcttt taaatattca
    11581 atcacctctg ttttgtataa gccattcacc gtttcagcca aagcattatc gtatgaatca
    11641 cccgttgtac cgactgatgc tcgtaaattt gctgcttcta aacgattggt atagcgaatg
    11701 gaaagatatt gcacacctct gtcggaatga tgaatcacgt tctttggcat gcctcgatca
    11761 tgcaatgctt gctccagtgc atcaagtacc atatctgtat tcatccgtgt tgatactttc
    11821 catccaacaa ttgctcgcga gaacacatca ataataaatg cagtgtagac ccagccagaa
    11881 ttggtttgaa tatacgtaaa gtcactgacc cataattggt caggtcgatc agcactaaaa
    11941 ttccgtttca ctaaatcatc tgctcgtttt tgatcatctc ggctgcgggt agtttgtttg
    12001 ttcttaccac gccaaacacc ttgtatatct agcttctgca tcaatcgagc aactgtacaa
    12061 cgtgcaataa catagccttc acgtttcaat ttttgccaaa ctttacgtac accatatcga
    12121 cctaaacttt ctttccaaat acgtttgatt tgctctgcat gatgtaaatc atgcagagca
    12181 cgtttcgctc gatgttctgg gttatcaacg agatctaaag cccgataata ggtcgaagct
    12241 gcaatcggta aaattctaca aatcgcttca acaccatatc gatctttatt gttatggata
    12301 aaatccacca ttatttgtgt gggcggtcga gctccgcctg ggcgaaaaaa gcggctgctt
    12361 tgcgtagaat ttcattggca cgctttaatt ctttaatttc acgttccatt tgcttcattt
    12421 tttcttggtc agatatctgt tgtactttgg caggatttag ttgatccata tgctttaaat
    12481 accaaacacg caatgtttca ggagtacaac cgattttagg agcaatagct gtgatcgcag
    12541 cccaattcga tggataatct ttttcagatt caatcaatag ttgaaccgct ctttctctga
    12601 tttcaggggt atattttaat tttgtcatcg ggatagtctc tcagaatatt gactctccga
    12661 caaacccggt acggttca
//
