LOCUS       NZ_CP048663            13003 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Acinetobacter piscicola strain YH12207_T plasmid pYH12207-4,
            complete sequence.
ACCESSION   NZ_CP048663
VERSION     NZ_CP048663.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13979601
            Assembly: GCF_015218165.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter piscicola
  ORGANISM  Acinetobacter piscicola
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 13003)
  AUTHORS   Chen,C., Sun,J., Liao,X.-P. and Liu,Y.-H.
  TITLE     Tigecycline-resistant Acinetobacter species from pigs and migratory
            birds
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13003)
  AUTHORS   Chen,C., Sun,J., Liao,X.-P. and Liu,Y.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2020) College of Veterinary Medicine, South China
            Agricultural University, Wushan, Guangzhou 510642, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 11:34:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,216
            CDSs (total)                      :: 4,108
            Genes (coding)                    :: 3,944
            CDSs (with protein)               :: 3,944
            Genes (RNA)                       :: 108
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 67 of 164
            Pseudo Genes (incomplete)         :: 118 of 164
            Pseudo Genes (internal stop)      :: 42 of 164
            Pseudo Genes (multiple problems)  :: 51 of 164
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048663.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13003
                     /organism="Acinetobacter piscicola"
                     /mol_type="genomic DNA"
                     /strain="YH12207_T"
                     /isolation_source="feces"
                     /host="Pig"
                     /db_xref="taxon:2006115"
                     /plasmid="pYH12207-4"
                     /geo_loc_name="China: Zhejiang"
                     /lat_lon="29.08 N 119.65 E"
                     /collection_date="2017"
                     /collected_by="Chong Chen"
     gene            complement(join(12052..13003,1..833))
                     /locus_tag="G0028_RS20620"
                     /old_locus_tag="G0028_20600"
     CDS             complement(join(12052..13003,1..833))
                     /locus_tag="G0028_RS20620"
                     /old_locus_tag="G0028_20600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="WP_194088772.1"
                     /translation="MVKRISIVISTLLLYPNIAMAIQGGSSSSDITQIDANNNIASGPS
                     ASAGSSGAITSSPNGMAGIGGNNNIVLGSGNSGDGGDGGGASRHGAIGGAGGNNNIVLG
                     SGVSGIGGNAVNTVTDWTYGGAGGSNNISIGGGTTGVGATVVQTSGTGGTGGSYNLAIG
                     TNSYAGNANGFTSGNNGGIAIGSSDTVSTTASGTNSIALGTGSVSSGKNSVSIGSSSSD
                     GGLENVVSVGSSAQTRKIVNVTAGDVNNFSTESVNGAQLFTTNQAVSTAQTTANTAKTT
                     ADSALTNAATAQTTANTAKTTADSALTNAATAQTTANTAKTTADSALTNAATAQTTANT
                     AKTTADSALTNAATAQTTANTAKTTADSALTNAATAQTTANTAQTTADSALTNAATAQT
                     TANTAKTTADSALTNAATAQTTANTAKTTADSALTNAATAQTTANTAKTTADSALTNAA
                     TAQTTANTAKTTADSALTNAATAQTTANTALNNAATANHRIDILDNKFQKIENRLNKID
                     TKLDSVGALAAAQSRIPAVIDGSKNYAVSIGIGNYGSKNAMAVGATIKINENIFSSGSF
                     AIADHDSSSSSLGVSFAW"
                     /name="YadA C-terminal domain-containing protein"
     gene            complement(1413..2531)
                     /locus_tag="G0028_RS20625"
                     /old_locus_tag="G0028_20605"
     CDS             complement(1413..2531)
                     /locus_tag="G0028_RS20625"
                     /old_locus_tag="G0028_20605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004755996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="WP_180047661.1"
                     /translation="MQKWIWQAENWTNFTWQDSTILPKTRQIHQKIGILLGQSQHDLAK
                     EQFTLDTLLANLVASSAIENETLNVFSLRSSLAQRLGVTLEQPYPSSDRSEGLANIMLD
                     ALNDVKQPLTVERLMQWHRWLFNDPDWTMQRLRIGQLRGPEPMQVVSGRLDYPKVHFEA
                     PPREGLAERLNAFIGWFNHSLDDSLLDPLLRAGITHLWFVTLHPFDDGNGRITRTLTDL
                     ALAQMDEQSIRLYAMSTAILSKRKSYYEILEQTQKNDSDITDWLVWFLDTLNDSLEKTL
                     AQINRTLFKSQFWHKYSNLALSEEQRKVLNRLLDGGENGFEHGISASQYQKVAKVSKAT
                     ATRHLSDLLEKACIVKLEGGGRNTRYQINTQL"
                     /name="Fic family protein"
     gene            2733..3260
                     /locus_tag="G0028_RS20630"
                     /old_locus_tag="G0028_20610"
     CDS             2733..3260
                     /locus_tag="G0028_RS20630"
                     /old_locus_tag="G0028_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="WP_180047663.1"
                     /translation="MGTVKLKSSDEVFQLKISLLGSNPEIWRTIQISSAATLSRLHKAI
                     QSAFDWEDSHLHEFTTIKHEKINKRQKLKEVLRVGKKIIYTYDFGDCWEHLITVESRQS
                     PDLNKKYPCCIDGQNHAPFEDIGGIFGYLDILDALQDSKHPRYDDYMQIIEDEIGEIEF
                     DPTYFNSHDIKF"
                     /name="plasmid pRiA4b ORF-3 family protein"
     gene            3366..3614
                     /locus_tag="G0028_RS20635"
                     /old_locus_tag="G0028_20615"
     CDS             3366..3614
                     /locus_tag="G0028_RS20635"
                     /old_locus_tag="G0028_20615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004812233.1"
                     /GO_function="GO:0003729 - mRNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family toxin"
                     /protein_id="WP_004812233.1"
                     /translation="MGKTEKLLEKFGNSKNTFPYKDLVVLLSQLGYEKFEMAGSRVRFF
                     NEKTEHMILLHRPHPENEIKGGALKAVKQALKQEGFL"
                     /name="type II toxin-antitoxin system HicA family toxin"
     gene            3611..3943
                     /locus_tag="G0028_RS20640"
                     /old_locus_tag="G0028_20620"
     CDS             3611..3943
                     /locus_tag="G0028_RS20640"
                     /old_locus_tag="G0028_20620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004812232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="WP_004812232.1"
                     /translation="MSYLKYKGYLGTIEPDLETGELFGKLAFIRDLITYEAETLKALEK
                     AFQESVDGYLESCAELGKSPDQPFKGTFNVRISPELHRKAVLASSNSLNAFVSDAIQEK
                     LMRLGA"
                     /name="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            4073..4408
                     /locus_tag="G0028_RS20645"
                     /old_locus_tag="G0028_20625"
     CDS             4073..4408
                     /locus_tag="G0028_RS20645"
                     /old_locus_tag="G0028_20625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_179997550.1"
                     /translation="MKIGIFWFLQKQVIGIAHPFNLNDADSIGLIDSPYTHVDYWKNMQ
                     SVYPELRHYEYEQIPRGRVIFDTNKGKAIVYMDKKLFNTVTATKIYDFFDIDSENAIPR
                     KDPHYRT"
                     /name="hypothetical protein"
     gene            4458..4637
                     /locus_tag="G0028_RS20650"
                     /old_locus_tag="G0028_20630"
     CDS             4458..4637
                     /locus_tag="G0028_RS20650"
                     /old_locus_tag="G0028_20630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001231652.1"
                     /translation="MRKRLKDYSLDQISNREKVSGQVLPIQFSNDEATKRVVMHSAKRV
                     INQHKAEIQELAYK"
                     /name="hypothetical protein"
     gene            4634..5020
                     /locus_tag="G0028_RS20655"
                     /old_locus_tag="G0028_20635"
     CDS             4634..5020
                     /locus_tag="G0028_RS20655"
                     /old_locus_tag="G0028_20635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004769969.1"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="WP_000053135.1"
                     /translation="MSLISSTFVIAVHDEILLQTGVGRIGCHIEKLESVLSRIDQQMYY
                     SNVSDIFEIAAWFGIAISKGHAFVDGNKRTGLAVMLTYLEIQGVTIQEQTGLDDLMVDI
                     VESQEEHELLAKIVAEFLYELSII"
                     /name="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(5022..5573)
                     /locus_tag="G0028_RS20660"
                     /old_locus_tag="G0028_20640"
     CDS             complement(5022..5573)
                     /locus_tag="G0028_RS20660"
                     /old_locus_tag="G0028_20640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="WP_180042379.1"
                     /translation="MKKVHLIHADITAFAVHAIVNSANKSLLGGGGLDYVIHKKAGPLM
                     KEECIRLNQEKGGCPTGQAEVTTAGNLPAKYLIHAVGPRWLDGEHNEPQLLCDAYSNAL
                     FKANEIHALTVSFPCISTGVYGFPPQKAAEIAIGTILSMLPQYDHVAEVFFICREDENY
                     LIYKNLLSNIDDPNIQILIS"
                     /name="macro domain-containing protein"
     gene            complement(5582..6280)
                     /locus_tag="G0028_RS20665"
                     /old_locus_tag="G0028_20645"
     CDS             complement(5582..6280)
                     /locus_tag="G0028_RS20665"
                     /old_locus_tag="G0028_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005401798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_180047665.1"
                     /translation="MEKSLNLANSIFAGFNDKNGLMICGYEWGWSKEDQKLDESDSRPT
                     VDMSIECTFSNKSLRYGPSANTWPYDKNIKKWFKFWGHPLNDENLGSDFDKCIIQTNWA
                     YTSNADIESYNRFLEKEAVDNFIHHIEVLRPKVILFMGSKLINFLRNIDVWDRFTAIVG
                     PEIEPLKRVQKTDFDGTRFKVYFDNFEQCQVVCLPHPSSSRGLSDEYIKLFEPELGTII
                     TEYKKQKGIL"
                     /name="hypothetical protein"
     gene            complement(6634..6858)
                     /locus_tag="G0028_RS20670"
                     /old_locus_tag="G0028_20650"
     CDS             complement(6634..6858)
                     /locus_tag="G0028_RS20670"
                     /old_locus_tag="G0028_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004758944.1"
                     /translation="MPYTEFQRLVGKAGLSIKEFAALLDMKPNSITNYSKQGVVPTHIA
                     VIVALISTMKDEGLDFYPIFEKVKSYSQE"
                     /name="hypothetical protein"
     gene            7012..7257
                     /locus_tag="G0028_RS20675"
                     /old_locus_tag="G0028_20655"
     CDS             7012..7257
                     /locus_tag="G0028_RS20675"
                     /old_locus_tag="G0028_20655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004282220.1"
                     /translation="MYSYPDSNTEKKIALMIINDFFIQKAHELWIFLQLDQCFNDYEAT
                     LIWTRRYLEKHPECEYSDIQKAFVSCFPEHFFSFDC"
                     /name="hypothetical protein"
     gene            complement(7547..8308)
                     /locus_tag="G0028_RS20680"
                     /old_locus_tag="G0028_20660"
     CDS             complement(7547..8308)
                     /locus_tag="G0028_RS20680"
                     /old_locus_tag="G0028_20660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_057073610.1"
                     /translation="MEMEKAKKRGRPAQLLQIAELHAFVEFLEQQEQLSDLQSQVLKAL
                     NSVDCNFEGLTQTDQVLVKEALKPYREHLKLKLLFEELNNLPLKTEYEQKFLDLYELFQ
                     KNALDQMELNILKTLATRYLNFKAQKLEYSDLELYLSQLQKKDAGKKRKAENQRKFELG
                     GAVLVAFKKLNIDISNDTPQQITNRIVNTTKLHNEVRKSLIFKDVKTYKNEYFEANKLF
                     IQVLEGLHTWQKGGELLSVIEIKKALEKGEE"
                     /name="hypothetical protein"
     gene            8613..9782
                     /locus_tag="G0028_RS20685"
                     /old_locus_tag="G0028_20665"
     CDS             8613..9782
                     /locus_tag="G0028_RS20685"
                     /old_locus_tag="G0028_20665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_180047667.1"
                     /translation="MASYHFSVKSKNKGYATGHYLYISRLMQYEGIRKTSNETIEHIEP
                     GRCMPAFVKDPIDFWQAADTYERANAKAYMEYEIALPNEFTPEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERTAEQYFKRYNPKDPSKGGAKK
                     IQLQDGHEDYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEIHSTLYLPEPKLGAGQQNATPEYLTQIQKIREHNQQERELEERQKQLDQQHEYFYSA
                     ENHPFSSLEVMYYLNYLVKNQKNKEKLETDFSEIIKTQEPILHQEGWNLQNMNRKLRDS
                     EHNSTPCFEFEVYSQPKYPLPMQNKPPQPVQPEQPKPKSDDYDYGGLDF"
                     /name="MobA/MobL family protein"
     gene            10330..11211
                     /gene="repM"
                     /locus_tag="G0028_RS20690"
                     /old_locus_tag="G0028_20670"
     CDS             10330..11211
                     /gene="repM"
                     /locus_tag="G0028_RS20690"
                     /old_locus_tag="G0028_20670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004734502.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005804946.1"
                     /translation="MNKNHVVKSNQVIEASYQLSAVEQRIVLAAISRIPKNQPITDDEL
                     YPVSVDELKQLGVHEKTAYRDLKEGINRLYERSINLSIDDKSIKMRWVQEIQFLDSQSV
                     IGIRFSKPILPFISNLSREFTKYALSDIAGINSGYGIRIYELLVQYRQIGKREISVESL
                     RTMLELGKKYPLFADFKKRVIDTAIDQINEYSPLKVTYEQKKTGRKVTHIIFSFKEKSK
                     SITHTSKDFYQLSDAQINLFGNQLSRLHELSHLASEGESYEILAAKIKDMLRNPHQQQQ
                     FLPHLKNLGFKA"
                     /name="repM"
     gene            11275..11841
                     /locus_tag="G0028_RS20695"
                     /old_locus_tag="G0028_20675"
     CDS             11275..11841
                     /locus_tag="G0028_RS20695"
                     /old_locus_tag="G0028_20675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_180047671.1"
                     /translation="MKTSTKYSVSDAATLYKKSRATLYKDIKNGVLSRDHDGSIDFSEL
                     LRVYGEPFSGKHSKHTDTSPTQTINTPEYTDNTVIELKNQIDFLKKQLEKAEDREAKAN
                     SRIDTLLTLIEMKPTQVHPTPTSDQSSVQSSPSQVEPTLNVDNDKHDGLTTPEQTENKR
                     IPVPEPVEPEQPKRGWLSRFFLPNG"
                     /name="plasmid replication DNA-binding protein"
     Typing          10331..11209
                     /name="rep_cluster_1364"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.922"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          8614..9893
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.588"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gctgtgtttg ccgttgtttg agctgtagaa acagcttggt tcgttgtaaa tagctgtgct
       61 ccatttactg actctgtaga aaaattatta acatctcctg cagttacatt aactatttta
      121 cgagtttgtg ctgaacttcc tacactaaca acattttcta aaccaccatc actacttgaa
      181 gaaccaattg aaacagagtt tttccctgat gaaactgagc ctgtacctaa tgctatagaa
      241 ttagttccac ttgctgttgt tgatacagta tctgagctgc caatagctat tccaccatta
      301 tttcccgagg taaatccatt tgcattgcca gcataactat tagttcctat tgctaaatta
      361 taagatcctc ctgttccgcc agtacccgaa gtttggacta cagttgcacc aacaccagtt
      421 gtcccgccac caatagaaat attattactt ccaccagctc caccatatgt ccaatcagtt
      481 acagtattaa ctgcattccc tccaattcca gaaacaccac tacccagaac tatattatta
      541 tttcccccag ctccaccaat agcgccatga cgtgatgcac caccaccatc accaccatca
      601 ccactatttc cagatcctaa aactatatta ttatttcctc caattcccgc cattccattt
      661 ggggagctag taatagcacc actacttcct gcagacgcac taggacctga agctatatta
      721 ttattagcat ctatttgagt aatatctgat gaactacttc caccctgaat agccatagca
      781 atatttggat aaagcaatag agtacttatt acaattgata ttcttttaac catcttaaaa
      841 tcatcttgag ttttgaggtt acaaaaatat aataaataat atagttttac attaaatatc
      901 tttatagcct gttagaccat tataagtgaa ccccacattt aacataatgg gcgttatacg
      961 aaatgctttt taaaatatct ttgtttttca tatagttaaa aaattttcat ccccctaaaa
     1021 aaccacaaaa aaccgacttg gaaacaagtc ggtttctttt taagcaaaac tgtaactttt
     1081 agccaataaa attgatcaat atttaatcaa attgtcattt ttctctggct tatcaatgcg
     1141 ccccatcccg aaactatccc caatttctgc ggacaactct tgggataaaa attcaccaat
     1201 tttgtacgct ttggctcaca ccaattccgg ccctttgcgc ctatacgatc tggctacaat
     1261 ttcctatgat ggattcatca ggaacaggaa gttccgcaca ataataaaaa tacagatacg
     1321 cacaaggaca gtaaggtacg acaggagtta taccaagcga acccatacga aaaagcccga
     1381 catcttgtcg ggctttttta ttgcttaaat atctataact gagtattgat ctgatagcgt
     1441 gtattacgtc caccaccttc caatttgacg atacatgctt tttcaagcaa gtctgataaa
     1501 tggcgagtag ctgtcgcctt actgacttta gcaacctttt gatattgtga agcactaata
     1561 ccatgttcaa agccattttc tccaccgtct aataaacgat ttaggacttt gcgttgttct
     1621 tcactcaggg ctaagtttga atatttatgc caaaactgac ttttaaataa tgttcgattg
     1681 atctgcgcta aggttttttc aaggctatca ttcaatgtat ctaaaaacca aactagccaa
     1741 tctgtgatat cagaatcatt tttttgagtt tgttctaata tctcatagta acttttacgt
     1801 ttacttaata tcgcagtaga cattgcatac aaacggatac tttgctcatc catctgagct
     1861 aaagcaaggt ctgtaagtgt acgtgtaata cggccattac catcatcaaa aggatgtaga
     1921 gtaacaaacc ataaatgggt aatgcctgca cgtaataaag gatcaagcag tgaatcatct
     1981 aggctgtgat taaaccaccc tataaaggca tttaatcgct ctgctaagcc ttctcttggc
     2041 ggtgcttcaa aatgtacttt agggtaatcc aatcgacctg aaacgacctg cataggctct
     2101 ggtcccctaa gttgaccaat gcgtaaacgt tgcattgtcc agtcggggtc attgaaaagc
     2161 caacgatgcc attgcatgag gcgctcaaca gtcagtggtt gcttaacatc gttcagcgca
     2221 tccagcatga tattggctaa accttctgat cgatctgagc ttggataagg ctgttcaaga
     2281 gtgacaccca agcgttgggc taaagatgat cgcaatgaaa aaacattgag ggtttcattt
     2341 tcaattgctg atgaagcaac gagattggca agtagagtat ctagagtgaa ttgttctttc
     2401 gctaaatcat gctgactttg tccaagaaga attcctattt tttgatgtat ctgtctggtt
     2461 ttaggtaaga tagttgaatc ttgccatgtg aaatttgtcc agttttctgc ttgccaaatc
     2521 catttctgca ttttcaatct ctctcatcta aaatctaaga tgagttaaat atagcacata
     2581 ttcgactcac attatgagcc gaatatgtgc tattaattag ctcattattt atcgagaaca
     2641 gttctactta ggttctaaca agtgagtttc gtataacgcg tattatgtta attttagatt
     2701 tactaaaact attttttgag aactaggctg ttatgggaac tgtaaagctc aaatcttctg
     2761 atgaagtttt tcaattaaaa attagtctgc taggtagtaa tcctgaaatc tggagaacta
     2821 ttcaaatttc atcggcagct actttatcta ggctccataa ggcaattcaa tctgcttttg
     2881 attgggaaga tagccattta catgaattca cgactattaa gcatgaaaag atcaataaaa
     2941 gacaaaaatt gaaagaagtt ctacgtgtag gtaaaaaaat tatatataca tatgattttg
     3001 gcgattgttg ggaacatttg attactgtag aaagcagaca atctcctgat ttaaataaaa
     3061 aatatccatg ttgtatagat ggtcaaaatc atgctccatt tgaagatatt ggaggaattt
     3121 ttggatatct tgatatttta gatgcattac aagactccaa acatccaaga tatgacgatt
     3181 acatgcaaat tatcgaggat gaaataggag agatagagtt tgatccgacc tactttaatt
     3241 cacatgatat taagttttaa acctgattta acataagggc tgttatacga aattgcaact
     3301 aaatatagtt gcaattatta tttaaaagta actatactta gttgcaatag cagtagggct
     3361 gaagtatggg taaaacggaa aagttactgg aaaaatttgg aaattcaaaa aatacgtttc
     3421 catataaaga tttagtcgtt ttactgagcc agctcggcta tgaaaaattt gaaatggcag
     3481 gctctcgtgt tcggtttttt aatgaaaaaa ctgaacatat gattttactg catagaccac
     3541 accctgaaaa tgaaatcaaa ggtggggcat tgaaagctgt aaaacaggca ctcaagcagg
     3601 agggattttt atgagctatt taaagtacaa aggttatctc ggaacaattg agcctgatct
     3661 tgaaacaggt gagttatttg gaaaacttgc ttttattcgt gatcttatta cctatgaagc
     3721 ggaaacttta aaagcccttg agaaagcttt tcaggaatct gtagacggct atttagaatc
     3781 atgtgctgaa ctaggaaaat ctcctgatca accattcaaa ggcactttta atgtccgtat
     3841 tagccctgaa ttgcaccgta aggcggtttt agcatcaagt aattctctaa atgcctttgt
     3901 cagtgatgcg attcaagaaa agttaatgcg tttaggtgcg taatttattg aagcctaggc
     3961 tactacctaa aaattgaaaa actcagcaaa aatgggagct taaagctccc atttttagtt
     4021 tcgtataagg tgtattatgt taattttagg gaaattaatc agttgatgaa aaatgaaaat
     4081 aggaattttt tggtttttgc aaaagcaagt tattggcata gctcatccat tcaatttaaa
     4141 tgatgcagat tcaataggct taattgatag tccatataca catgtcgatt attggaaaaa
     4201 tatgcaaagt gtatacccag aactacgtca ttatgaatat gaacagattc caaggggtag
     4261 ggtaatcttc gacacaaata aaggaaaagc aatcgtgtat atggacaaaa aattatttaa
     4321 cacagtaaca gccactaaaa tttatgattt ctttgatata gattcagaaa atgctattcc
     4381 aagaaaagat cctcactatc gtacttaatt gtttaattta ggctaaaatg aaattctctt
     4441 ttaatagggg gatgaagatg agaaagcgtt taaaagacta tagccttgat caaatttcaa
     4501 atcgtgaaaa ggtttcgggt caagtattac caatccaatt tagcaatgac gaagcgacta
     4561 aacgtgttgt gatgcatagt gcaaaacgag tcattaatca gcataaagct gaaatccaag
     4621 agcttgctta taaatgagtt tgattagctc aacattcgtc attgctgtgc atgatgagat
     4681 cctattacaa actggcgtag ggcgaatagg ctgtcatatt gaaaagctag aaagcgtatt
     4741 aagccgtata gatcagcaga tgtattacag taatgtaagc gacatttttg agatcgcagc
     4801 atggtttggt atagctatct caaaaggtca tgcttttgtt gatggtaata aaagaactgg
     4861 tttagctgta atgctgactt atctcgaaat tcaaggtgtt accattcagg aacaaacagg
     4921 tttagatgat ttgatggtcg atattgttga atcacaagaa gaacatgaat tattagccaa
     4981 aattgtggct gaattcttat acgagctttc gataatttaa actaagaaat cagaatctga
     5041 atatttggat catcgatatt cgataaaaga ttcttataaa ttaaataatt ttcatcctct
     5101 cggcagataa aaaatacttc tgcaacatga tcatattgag gcaacataga gagtatagtg
     5161 ccaatcgcaa tttcagcagc cttttgaggt ggaaaaccat aaacacctgt actaatacat
     5221 ggaaaagaaa cagtgagtgc atgaatttca ttagctttga atagtgcatt actataagca
     5281 tcacaaagca attgtggctc attgtgttcg ccatctaacc atcgtggtcc aaccgcatga
     5341 ataaggtatt tagcaggcag attacctgct gttgttactt ctgcttgacc tgtagggcat
     5401 ccaccttttt cttgatttag acgaatacat tcttctttca tcaacggtcc agctttttta
     5461 tgaatcacat aatcaagtcc cccaccacca agcaaagatt tatttgctga gttaacgatg
     5521 gcatgtacag caaaagctgt tatatctgcg tggatgagat ggactttttt cataattagt
     5581 tttatagaat acccttttgt ttcttatatt cagtaatgat tgttcctaat tctggttcaa
     5641 ataatttgat atattcatca gatagacctc tactactaga tggatgtggt aagcacacaa
     5701 cttggcattg ttcaaaatta tcaaagtaaa ctttaaatcg agtaccatca aaatctgttt
     5761 tttgtaccct ttttaagggt tctatttctg gaccaacaat agcagtaaac ctatcccaaa
     5821 catctatatt tctcagaaaa ttaataagct tactacccat aaataaaatt actttgggtc
     5881 tcaaaacttc aatatgatga ataaaattat caactgcttc tttttctaag aaacgattat
     5941 agctttctat atctgcatta ctcgtatagg cccaattagt ttgaataata catttatcaa
     6001 agtctgaacc taaattttca tcgtttaatg ggtgtcccca aaacttaaac cattttttaa
     6061 tatttttatc ataaggccaa gtattggcag aaggaccata ccttagggat ttattactaa
     6121 atgtacattc aatgctcata tctactgtgg gacggctatc gctttcatca agtttttgat
     6181 cttcttttga ccatccccat tcatagccac aaatcattaa tccgttttta tcattaaagc
     6241 ctgcaaaaat tgaatttgct aaatttagac ttttttccat tttatcttct ctgatacttt
     6301 taagattcgc agattcaaat tatcacatat atttcatata aatcatttat atggaaagtt
     6361 ttttatagat ataaaaatac acttaacata aaatctctta tgcgaaattc agttgtaacc
     6421 cccaaataga aatgtccggt ttatcgattg ctcgacctaa gatctcagaa cataagaatt
     6481 tgatgatcac cctggttctc aaaactcgat actttattga aaagttctat cgaggcttat
     6541 aagtccctga aacaaaaaaa tgcctgaagt ttaggcattt tttacatcat caatatctac
     6601 taaaaagctt acaacaatca aaagacgaga gttttattct tgtgaatacg acttaacttt
     6661 ttcaaaaatt ggatagaaat ctaatccctc atctttcatc gtagaaatca acgcaacgat
     6721 tacagcaata tgtgtaggaa ccacaccttg tttactgtaa ttcgtaatcg aattaggttt
     6781 catatccaat aaggcagcaa attccttaat tgaaagccct gctttaccga caagacgttg
     6841 aaattcagta tatggcattt aattaaattt ataaaaatgg tgttttgaga ttatagcata
     6901 gggttatact tacataaaaa cacataaaat tatgtggcaa acacatattt ttatgtgtta
     6961 taaatagctt atcaactaac ctatttatcc ttactatcgg agaaatgtaa tatgtactca
     7021 tacccggatt caaacactga aaagaaaatt gctctcatga tcatcaatga ctttttcatt
     7081 caaaaagccc atgaactgtg gattttttta caactcgatc agtgctttaa tgattatgaa
     7141 gccacactga tttggactcg acgttattta gagaagcatc ctgagtgtga gtacagcgat
     7201 attcaaaaag cctttgtttc ttgtttccca gagcacttct tcagctttga ctgttaattt
     7261 atgagaggca cggtaaaaaa tcgcaatggg taagtaaatc ccaaatagaa agagccagtt
     7321 catacaactg gctttttatt gtcacgtata aatctaagac tttctttgag catactaaaa
     7381 ttgatgttcc acggcttcta agacattgat tttaggttca ggattggggt cgccatgacg
     7441 ttactttagg gtgtattcta aatttacata atgtctctgt atacgaaatt cgattgtaag
     7501 cccaaaatag aaatgtccaa cttctctaaa atccctgcta aataaactac tcttcgcctt
     7561 tttctaaggc ctttttaatt tcaattactg acaataattc tccgcccttt tgccaggtat
     7621 gtaatccttc taacacctga ataaaaagtt tatttgcttc aaaatattca tttttatatg
     7681 tcttaacatc tttaaaaatt aaagatttac gtacttcatt atgaagcttt gtcgtattca
     7741 caatccgatt ggtaatctgt tgaggagtat cattgctaat atcaatattg agtttcttaa
     7801 atgcgacaag aacagcacca cctaattcaa atttacgttg attttcagct ttgcgttttt
     7861 tcccagcatc cttcttctgt agctgactca aatacaattc taaatcactg tattcaagct
     7921 tttgcgcttt aaaattcaga taccgtgtcg ccaaggtttt taaaatattt agttccatct
     7981 gatctagtgc attcttttga aaaagttcat acagatctaa aaacttctgt tcatactcgg
     8041 ttttaagagg cagattattc aactcttcaa acaacaattt caacttcaga tgttcacgat
     8101 aaggttttaa agcctctttg accagaacct gatctgtctg agttagcccc tcaaaattac
     8161 aatcaacact atttaaagcc tttaacactt gagattgaag atcagaaagc tgttcttgct
     8221 gttctaaaaa ctctacaaat gcatgtaact cagcaatctg taacaactga gcaggtcgac
     8281 cacgcttttt tgctttttcc atttccatca tctgccgcca aattttcaga actgcaacct
     8341 cttcaaatat ggttttacca tatttaaaga gggcagtctg aaaataatta tttaaattaa
     8401 aaataaaatt tcttgaaaag aatttttatt tttaatttaa ataattcgca cttgacagtt
     8461 tctcttgatc agagaaacta gtacgcaatg gggcgcttcc cccttgaccc ccttcaaccg
     8521 gatttttcag ccattgaggc attgtaaatc cggacggttt tcattccgtt tcactgcata
     8581 aaaaccgaac ccacaaccca gcagaggtga acatggccag ctaccacttc agcgtcaaaa
     8641 gcaaaaataa gggctatgcg accggccatt atctctacat ttcccgactg atgcagtatg
     8701 aaggcatccg caaaaccagc aatgagacca tagaacatat tgaacctggt cgatgtatgc
     8761 ctgcctttgt aaaagatccc attgattttt ggcaggcagc agatacgtat gaacgagcca
     8821 atgccaaggc ctacatggag tatgaaattg ccctaccaaa tgagttcacg ccagaacagc
     8881 gtaaaacgct catagagaca tttttggaca aacacatagt cccacagcaa tatccgcatt
     8941 catacgccat acacaacgta aaatcacgca tcagcggcga agatcagccc cattgtcacc
     9001 tgatgttctc gctcaaagcc gatgacggta ttgaacggac cgccgaacaa tatttcaaac
     9061 gctataaccc caaagatccc agcaaaggcg gcgcaaagaa aatccagctt caagatggcc
     9121 atgaagacta ttccactttt ttgatctata tccgaaaaca atgggaaaac catctgaatg
     9181 atgcccttgc acagcactgt cctacagtca cttacacact ggatggccaa gacatcacca
     9241 tcaaaaatca ggtttcagca gacagctatg aaaaatataa cgagatccac agcacgttat
     9301 accttccaga gccaaaacta ggcgctggtc aacaaaatgc gacaccggaa tatctaactc
     9361 agatccaaaa aatccgagaa cacaaccagc aagaacgaga actcgaagaa cgccaaaagc
     9421 aattagatca acaacatgaa tatttttata gtgccgaaaa ccatccattt tcatcattgg
     9481 aagtgatgta ttacctgaat tatttagtga agaatcagaa aaataaggaa aaactagaaa
     9541 cagattttag tgaaattatc aaaacccaag agcctatatt gcatcaagaa ggctggaacc
     9601 ttcagaacat gaatcgaaaa ttgagagatt cagagcataa ctcaacgcca tgctttgagt
     9661 ttgaagtgta ttcccagcca aagtatccat tacccatgca gaataagccc cctcaaccgg
     9721 ttcaacctga acagccaaag ccaaaatctg atgattatga ttacggtggt ctagattttt
     9781 aaattatctg gttttattca ggccttacag agcgagtgtc tacgagcgaa cagactcaat
     9841 taaaattttc gccatgtttt ttccctgatt acaagctccc ctttgctgta gagatttagg
     9901 tgcagagcat cccgaagggt gcgaacagat tgaatgtgct tctaggccga gcgaagcgag
     9961 cataaaaaaa cttatgagca aagcgaattc cgagctgctt ttgctttttt gaagttatca
    10021 cgagattaac tcaaaaattg ccccttcgaa ctgagcgtaa gcgaagttca atagagtttg
    10081 agcgtagcga aaacataggg caatttttca ttctttcctt tttaatatat tttaaatctt
    10141 ttaaatcttt tcagtagtct ctgtgcctta tatagcaaag gtttcagcgt ttaaatatga
    10201 cggattgact actaactatg acggattgac tactaactat gacggattga ctacttaact
    10261 atgacggatt gactactaac tatgacaagt atatatttgt catatattaa aaaaatcaat
    10321 ggttttgttt tgaataaaaa tcacgttgta aaatcaaatc aagtaataga agcatcgtat
    10381 caattaagtg ccgtagagca acgaattgtc ttagctgcca tttcacggat tcccaaaaat
    10441 cagcctatta cagatgatga gttataccct gttagcgtcg atgaattaaa acaattgggc
    10501 gtacatgaga aaactgcata cagagattta aaggaaggta ttaatagact ctatgagagg
    10561 tctattaacc tcagtattga tgataaatct ataaaaatga gatgggtgca agaaatccaa
    10621 tttttagata gtcaaagtgt cattggaatc cgtttttcaa aacccattct gccattcatt
    10681 tccaatctaa gtagagaatt caccaaatat gccttatcag acattgctgg gatcaatagt
    10741 ggatacggta ttcgtattta tgagctactg gttcaatatc ggcaaatagg taagcgtgaa
    10801 atttctgttg agagtttacg gacaatgtta gaactcggta aaaagtaccc attattcgct
    10861 gatttcaaga aacgagtaat tgacactgct atagaccaaa tcaatgaata tagcccttta
    10921 aaagtcactt atgagcagaa aaaaacaggg cgtaaagtca ctcatattat cttttctttt
    10981 aaagaaaaat ctaaatccat aacacatacc tcgaaagatt tttatcagct atctgatgct
    11041 caaattaatc tattcgggaa tcaattatca cgtttacatg agctttccca tttagcctca
    11101 gaaggtgaaa gttatgagat tttagctgct aaaattaagg atatgcttag aaaccctcac
    11161 caacaacagc agtttttgcc acacttaaag aacttagggt ttaaagcttg agtgtattga
    11221 agaatacact tattgtattc ttcaatacgt atcctaaata cacagcaata cactatgaaa
    11281 acaagtacaa aatacagtgt tagtgatgca gccacactgt ataagaaatc tcgtgctaca
    11341 ctttataaag atataaaaaa tggggtgtta agtcgtgatc atgatggctc tatcgacttc
    11401 tctgagttac tccgcgttta tggtgaacct tttagtggaa aacactcaaa acacactgat
    11461 acgtctccaa cacaaactat aaatacacct gaatacacag acaatacagt tattgaacta
    11521 aagaatcaga tcgattttct aaagaaacag ttagaaaaag cagaagatcg cgaagctaaa
    11581 gcaaattctc gtattgatac attacttacc ctaattgaaa tgaagccaac tcaggttcat
    11641 cccacaccaa cttcagatca atcttcagtg caatctagtc catctcaagt agaacctaca
    11701 ttgaatgtag ataacgacaa acatgacgga ttgactactc cggagcagac ggaaaataag
    11761 cgtattccgg tgccggagcc ggttgaaccg gagcagccga aaagagggtg gttaagccgg
    11821 tttttcttgc cgaatggcta agggtactat tcaactgagc taatcaaagt tgtaaggatt
    11881 tgattaaaga atagccatat tacgtgaaag atcacaaaaa aaagcccgta cagggatgtt
    11941 cgggcaaaac tagggaacta cagcattgat ttctaaggta aattatatca cgtttcgtat
    12001 aacgtgtatt atgttaattt taggtaaatt taaaaatgaa cttttaaatt tttaccaggc
    12061 gaaacttact cctaaactac tactagatga atcatgatcc gcaatagcga aagatccaga
    12121 agaaaatata ttttcattaa tttttatagt tgcaccaaca gccattgcat ttttacttcc
    12181 atagttacca ataccaatac ttactgcata gtttttactt ccatcaatca ctgctggtat
    12241 ccgtgattga gccgctgcta atgcacctac actatcaagt tttgtgtcaa ttttattaag
    12301 acgattctca attttttgaa acttattatc taatatatca atacggtggt tagctgttgc
    12361 tgcattattt aaggcagtat ttgccgttgt ttgagctgta gcagcatttg ttaatgcgct
    12421 atccgctgtt gtcttagctg tgtttgccgt tgtttgagct gtagcagcat ttgttaatgc
    12481 gctatccgct gttgtcttag ctgtgtttgc cgttgtttga gctgtagcag catttgttaa
    12541 tgcgctatcc gctgttgtct tagctgtgtt tgccgttgtt tgagctgtag cagcatttgt
    12601 taatgcgcta tccgctgttg tcttagctgt atttgccgtt gtttgagctg tagcagcatt
    12661 tgttaatgcg ctatccgctg ttgtttgagc tgtgtttgcc gttgtttgag ctgtagcagc
    12721 atttgttaat gcgctatccg ctgttgtctt agctgtgttt gccgttgttt gagctgtagc
    12781 agcatttgtt aatgcgctat ccgctgttgt cttagctgtg tttgccgttg tttgagctgt
    12841 agcagcattt gttaatgcgc tatccgctgt tgtcttagct gtgtttgccg ttgtttgagc
    12901 tgtagcagca tttgttaatg cgctatccgc tgttgtctta gctgtgtttg ccgttgtttg
    12961 agctgtagca gcatttgtta atgcgctatc cgctgttgtc tta
//
