LOCUS       NZ_CP048653             4782 bp    DNA     circular CON 20-OCT-2023
DEFINITION  Enterobacter roggenkampii strain Ecl_20_981 plasmid unnamed3,
            complete sequence.
ACCESSION   NZ_CP048653
VERSION     NZ_CP048653.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13881485
            Assembly: GCF_014524505.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Zhou,K., Xu,T. and Zhang,C.
  TITLE     Novel colistin resistance gene in Enterobacter roggenkampii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Zhou,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2020) Respiratory Diseases Institute, The First
            Affiliated Hospital of Southern University of Science and
            Technology, 1017 Dongmen North Road, Shenzhen 518000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 400*x
            Sequencing Technology             :: Illumina oxford nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/20/2023 03:18:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,081
            CDSs (total)                      :: 4,966
            Genes (coding)                    :: 4,834
            CDSs (with protein)               :: 4,834
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 47 of 132
            Pseudo Genes (incomplete)         :: 89 of 132
            Pseudo Genes (internal stop)      :: 23 of 132
            Pseudo Genes (multiple problems)  :: 24 of 132
            Pseudo Genes (short protein)      :: 1 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048653.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="Ecl_20_981"
                     /isolation_source="Medical waste water"
                     /db_xref="taxon:1812935"
                     /plasmid="unnamed3"
                     /geo_loc_name="China: Shenzhen"
                     /lat_lon="22.5 N 114.2 E"
                     /collection_date="2019-10-08"
                     /collected_by="chuqiu zhang"
     gene            join(4760..4782,1..333)
                     /locus_tag="G3T53_RS25265"
                     /old_locus_tag="G3T53_25275"
                     /pseudo=""
     CDS             join(4760..4782,1..333)
                     /locus_tag="G3T53_RS25265"
                     /old_locus_tag="G3T53_25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /name="HigA family addiction module antitoxin"
     gene            complement(<653..775)
                     /locus_tag="G3T53_RS25680"
                     /pseudo=""
     CDS             complement(<653..775)
                     /locus_tag="G3T53_RS25680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            1426..1749
                     /locus_tag="G3T53_RS25270"
                     /old_locus_tag="G3T53_25285"
     CDS             1426..1749
                     /locus_tag="G3T53_RS25270"
                     /old_locus_tag="G3T53_25285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_163063187.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1958..>2392
                     /locus_tag="G3T53_RS25595"
                     /pseudo=""
     CDS             1958..>2392
                     /locus_tag="G3T53_RS25595"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2427..2918
                     /locus_tag="G3T53_RS25280"
                     /old_locus_tag="G3T53_25295"
     CDS             2427..2918
                     /locus_tag="G3T53_RS25280"
                     /old_locus_tag="G3T53_25295"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032629998.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            2925..3140
                     /locus_tag="G3T53_RS25285"
                     /old_locus_tag="G3T53_25300"
     CDS             2925..3140
                     /locus_tag="G3T53_RS25285"
                     /old_locus_tag="G3T53_25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3811..4053)
                     /locus_tag="G3T53_RS25290"
                     /old_locus_tag="G3T53_25305"
     CDS             complement(3811..4053)
                     /locus_tag="G3T53_RS25290"
                     /old_locus_tag="G3T53_25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            4240..4557
                     /locus_tag="G3T53_RS25295"
                     /old_locus_tag="G3T53_25310"
     CDS             4240..4557
                     /locus_tag="G3T53_RS25295"
                     /old_locus_tag="G3T53_25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          1138..1311
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          complement(663..1171)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          1427..1743
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.45e-59"
     Typing          1740..3232
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.2"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcaactgtag gggatattat tctttatgaa tatctcgaac caagtggttt aaaaatcaat
       61 gacttggctg aaatgttgaa tgtgcataga aactctgcca gtgctttagt taacaataat
      121 agaaagctgt ctaccgatat ggcttttagg ttggctaaag ccttcgatac cactgcaaag
      181 ttctggctaa atatacagca gtctgttgat ttatgggagg ttactagtag ccctcgattg
      241 caagaagaaa tagacagagt gattcccgct catgaaatca tagaaaggaa acacattgtt
      301 gatagttctg agaagaaaca tgctattgca tgattttttt taaaaaaata taagttatcc
      361 ctcaaaaaga aggcttctat aactagaagc cttctttttg agggataaaa acgctcatgt
      421 taactttctt cttacgatct tcttgagatc cgtgctctgt acgcgtaatc tcttgctctg
      481 taaacgaaaa aaccgcctgg ggaggcggtt tttcgaaggt taagtcagtt ggggaactgc
      541 ttaacctggt aactggcttc agcagagcgc agataccaaa tactgtcctt ccagtgtagc
      601 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      661 tcctgttacc agtggctgct gccagtggcg ttttgtcgtg tctttccggg ttggactcaa
      721 gtcgatagtt accggaacag gcgcagcggt cggactgaac ggggggttcg tgcatacagt
      781 ccagcttgga gcgaactgcc tacccggaac cgagtgtcag gcgtggaatg agacaaacgc
      841 ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg
      901 gagccatcag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga
      961 tttgagcgtc agattctgtg atgctcgtca ggggggcgga gcctatggaa aaccggctct
     1021 gccgtggcct tatcgcttcc ctgttaagtt tcttcctggc atcttccagg aaatttccgc
     1081 cccgttcgta agcccgcacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     1141 gcgaggaagc ggaatatatc ctgtatcgca tattctgctg acgcgccgtt gccgcctttt
     1201 ttctcctgcc acatgaagca cttcactgat ttccgtatcc gtgccaacat attaagccag
     1261 tatacactcc gctagcgcta cgtgactggt tcagggctgc gccccgaaac ccgccaaaag
     1321 ccactgacgc gcctgcggct tgtccaaccc cgcgccgacc gggaaaggct ttcccgcccg
     1381 tcccggcgtt atcaggaggc cggtttgtca gagaaacgga acaagatgct caccatgtgg
     1441 gtgacggagg gcgagcaccg gcggctgctt gagcgctgcg acggcaggcc gctggcggcg
     1501 tggatgcggc agacctgcct ggacgaaaag cctgcgcgca ccggaaagct cccctcactc
     1561 tcgccggcgc tgctgcgcca gcttgccggt atgggcaata acctgaacca gatagcccgc
     1621 cgtgttaacg ccggcggcgg caccggccat gacagggtac agattgtggc ggcgctgatg
     1681 gccatcgatg ccgggcttga gcggctgcgg cacgccgttc ttgagaaagg aacggacgat
     1741 gatcgttaaa tttcatccac gcggtcgcgg cggtggcgcc gggccggtgg actatctgct
     1801 gggtaaagac cggcagcgtg aaggcgccag cgtcctgcag gggaagccgg aagaagtccg
     1861 ggagctgatt gacgcgtcgc cttacgtgaa gaaatacacc tccggcgtac tgtcgtttgc
     1921 ggaggccgat ctgcctcccg gccagcggga aaaactgatg gccagcttcg agcgggttct
     1981 gatgcccgga ctcgataaag atcagtacag cattctgtgg gtggagcacg cggacaaggg
     2041 gcgtctggag ctgaactttc tgatccccaa taccgaactg ctgaccggca ggcggctcca
     2101 gccgtactac gaccgcgccg accggccgcg catcgatgcc tggcagacca tcgtgaacgg
     2161 cagactgggg ctgcatgacc cgaacgcgcc ggaaaaccgc cgggcgctgg tcacgccgtc
     2221 cggactgccg aaaacgaagc aggaggccac tgaagccatc acgcgcggac tgctgaccct
     2281 tgcttcgtcc ggggagctga aaaaccgtca ggacgtcact gaggcgctgg aacggtcagg
     2341 ttttgaggtg gtgcgcacca cgaaaagcag tatcagcatt gccgacccgg acggggggcg
     2401 aaacatccga ctgaagggag ccatctatga gcagtctttt aacgctggcg aaggacttag
     2461 agcagcaatc gaaagcgcag cagcagagta ccggcgagat gctgaaagcc gcattcagcg
     2521 agcacgagca gtctgtcaga gcggaactga gcgcaagcgc gaggagaatc agcgacgcca
     2581 tcctcgcaca cgagcagagt atgagcgagg cgatggagaa aaaccggcgg agcgtactgc
     2641 ggactgccgg gcggacatgg ttcaccatcc tgatggtgtc agcgctgctg atcggcacga
     2701 gcgggagcat actgtggtgg caggggcagc agataaccga caattacacg catcttcgtc
     2761 agcaggaaga taccctggcg aaaatgactg cccggacgtg gggagtgcgc tatcaggaga
     2821 gcagcgacgg ccggaggttt cttatcctgc cgcccgggat gcaaaccgaa gccatcccct
     2881 acgacgggac gacatggatc cgcctgaaac aggagtagcg gaaaatgaca gagctggaaa
     2941 agcagttgct gagcgcatta gagcagctac agcaggacta ctcgcaaagg ctggacgaat
     3001 gggagagcgc cttcgcggaa tggcggagca tgtgtgggct tatgcaacgg gagaacgcgg
     3061 tgctgaacga gcgcgtgtcg gacttgagca cgcaggtgct gagtttaagc gagcagctgc
     3121 gccgcttgtc cgggaactga atgccatcga gctccggcag agggagaatg agtatcagaa
     3181 aacactggag ctggagcgcc agccgcagaa aacctaccgc gggccgacac tttaaaccat
     3241 ccaggcagct gacaacccgc acgaagaggg gctcttattt ctcccgctaa ataggttcgt
     3301 gatcaaaaag gtcacgactt attcaggagc gggaaatgaa gctgttaaat ctcattgaat
     3361 cattgcttaa ctgcctgtat ctggcggcgg agctgtggga ctggctggag aagcatttgt
     3421 cggtttttaa ggcattggcg cggattttag tgtgacttta acatccatgc atctggctgc
     3481 atgactatgc accagtgtaa tattcagcgg ctgcgtttct ggtgtttttt cgttatgtct
     3541 gtcgccactt tctcgcgtct ggcaccggaa acgcagccaa ccccgtttca gcggtgcgcc
     3601 caacggatgt tatggtaaat cagtatcagc tgcagatata aaaaagccgc caggctggcg
     3661 gcttgctgtt attttcgtgg ctttcctgca tctgagcgtt ttcctctgac ccttccgtca
     3721 ttgttacggt cacgaggttg agttccgccg cctggtttag gaacgacaac gtgtgggttg
     3781 agtggtcgac gatctgccat tttctatacc tcactgagca taaacaaaca attgccaacg
     3841 aatgttccct tgcgcggggg tatgttggtg gtggttttgt ggtgggattg ttttcccgcc
     3901 tgcgatagtt acttctttac cgcaaccggt acatttataa atcccttcat attcacatgt
     3961 ttctcccggc ttatattcag tgtcaaaaac cgagttaaac ttcgtatctg cttcaaaaat
     4021 cttgcttttg tttcctgagc gaacaagagc catgcaacct ccaaattatc atttattttg
     4081 cacacctgca ctgtgcatga gtgcattcta cagcatagcc attgattgca caagtgcact
     4141 gtgctgttgt gcatttgata tgtttgctgt actatatcgg aggtaagaag gtgatcgaaa
     4201 aaattgccaa gttgcggcaa aaattgaggt aaaagaaaaa tgcagcaaga tcatgaacat
     4261 gatgattgcc gcattcagtt tcgagacgat tggctatacc agttttacaa atatggtaaa
     4321 gccaatcgaa agattccaac tgcgattagt gcttcattag gtcgcaagct ggatatgctg
     4381 aatgctgctg ataaagttgg agatttgcgg tctcccccgg gaaaccgatt tgagaatctc
     4441 aaccctccat tgacagggta ttcatccata cgggtgaatg agaagtaccg cttgatattt
     4501 aagtggggtg aaaacggagt cgaggatttg tatctcgatc cacatgatta ccgatagata
     4561 acaggtcgtg gctgttatct tataaaacaa gtaggtgagg cgttccctca cctacattaa
     4621 aatgtcatta caaataatga ttaataaaaa aaatgacgtg actatattta agataattac
     4681 tcatcgctac agctggtaat agacgaaata cttatcttcc tcatctaaca gagagaagta
     4741 aaaaatttag gagtaaagca tgtcccaagc aactagaaaa cc
//
