LOCUS       NZ_CP048652             3725 bp    DNA     circular CON 20-OCT-2023
DEFINITION  Enterobacter roggenkampii strain Ecl_20_981 plasmid unnamed2,
            complete sequence.
ACCESSION   NZ_CP048652
VERSION     NZ_CP048652.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13881485
            Assembly: GCF_014524505.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Zhou,K., Xu,T. and Zhang,C.
  TITLE     Novel colistin resistance gene in Enterobacter roggenkampii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Zhou,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2020) Respiratory Diseases Institute, The First
            Affiliated Hospital of Southern University of Science and
            Technology, 1017 Dongmen North Road, Shenzhen 518000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 400*x
            Sequencing Technology             :: Illumina oxford nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/20/2023 03:18:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,081
            CDSs (total)                      :: 4,966
            Genes (coding)                    :: 4,834
            CDSs (with protein)               :: 4,834
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 47 of 132
            Pseudo Genes (incomplete)         :: 89 of 132
            Pseudo Genes (internal stop)      :: 23 of 132
            Pseudo Genes (multiple problems)  :: 24 of 132
            Pseudo Genes (short protein)      :: 1 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048652.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="Ecl_20_981"
                     /isolation_source="Medical waste water"
                     /db_xref="taxon:1812935"
                     /plasmid="unnamed2"
                     /geo_loc_name="China: Shenzhen"
                     /lat_lon="22.5 N 114.2 E"
                     /collection_date="2019-10-08"
                     /collected_by="chuqiu zhang"
     gene            complement(join(3721..3725,1..757))
                     /locus_tag="G3T53_RS25245"
                     /old_locus_tag="G3T53_25255"
     CDS             complement(join(3721..3725,1..757))
                     /locus_tag="G3T53_RS25245"
                     /old_locus_tag="G3T53_25255"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_188074413.1"
                     /translation="MEFKIKKMTVKDSREIKKRFEDCIKIAEEKNMQVGSILNIEMQDR
                     LKEINLKVKKETAEQYRKLFNYKLSVNAEDIIQHLLECNTRNSFDKTRTAFRFCIAEKI
                     QELRNEADRARKQKNFDLMKEKTIEAYKLFFFFERDFLSENRVTWNDISYRKIESKSKK
                     KTMNSVSSIKAIFEDLKQKPELFDKYSMILAISSLTGCRPAEIQKGIGLELSPPLLNIC
                     ISGAKVGEDRGQEKRILKFNINEFAENEQFN"
                     /name="hypothetical protein"
     gene            complement(1131..1832)
                     /locus_tag="G3T53_RS25250"
                     /old_locus_tag="G3T53_25260"
     CDS             complement(1131..1832)
                     /locus_tag="G3T53_RS25250"
                     /old_locus_tag="G3T53_25260"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_139497357.1"
                     /translation="MTISKKNSELRERFKEYASNKNIFDLADLRYEILKIYCDFKLKSD
                     MSEKERKSQDSRRKAHLTALKKRIKREIVSKIVIDLVKYYNIEKTTFHFFSHICTEILE
                     RNVDNRYILNNFSNMILDEKKELRKLSESRNASNKMSLENSYSELVSMSHIKDKLFRND
                     NFKTAYLKCYGCANEEFSRFKVFAFPDNYETLDFLFEEERIKKEEKEISKIMIEQVEEE
                     QKIQPSKKRRL"
                     /name="hypothetical protein"
     gene            complement(1898..2146)
                     /locus_tag="G3T53_RS25255"
                     /old_locus_tag="G3T53_25265"
     CDS             complement(1898..2146)
                     /locus_tag="G3T53_RS25255"
                     /old_locus_tag="G3T53_25265"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_073568787.1"
                     /translation="MEIEKLIDRYFLALERKVIREIYNKHTREELEKFIFKCNSEFREF
                     EHRFNTICSTEYYEQQSLIFYINYINAEFEQREKLNY"
                     /name="hypothetical protein"
     gene            complement(3353..3718)
                     /locus_tag="G3T53_RS25260"
                     /old_locus_tag="G3T53_25270"
     CDS             complement(3353..3718)
                     /locus_tag="G3T53_RS25260"
                     /old_locus_tag="G3T53_25270"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_188074412.1"
                     /translation="MSKIKQNNKLEYKCSKQEYDSLRQYLYINHKGFSLYTLRHRAASE
                     LKKEGFTEETIAAFLGHRVTRSLENYGYARSGKGGPKIAGVEHSNAIKSNTKRFEKKTN
                     PGGVNPTPTRKRALKRF"
                     /name="site-specific integrase"
ORIGIN
        1 tgaactgctc attttcagca aattcattta tattgaattt caatattctt ttttcctgcc
       61 ctcgatcttc gcccactttc gccccgctaa tacatatatt taatagtggt gggcttagtt
      121 ccagcccaat tcctttttgg atctcagccg gacggcaacc ggtcaaactt gaaattgcta
      181 aaatcattga atacttatca aaaagctctg gcttttgctt caaatcttca aaaattgctt
      241 tgatacttga aacactattc atagtctttt ttttgctttt agattctatt tttctgtaag
      301 aaatatcatt ccaagttacg cgattttcgc tcaaaaaatc tcgctcaaaa aagaaaaaca
      361 acttatatgc ttcaatggtt ttttctttca ttaaatcaaa attcttttgt tttcttgctc
      421 gatctgcttc gtttcttagc tcttgtattt tttcagctat acaaaatcta aaagcagttc
      481 ttgttttatc aaaactgttt cttgtgttgc attctaacaa gtgttgaata atatcttctg
      541 cattcacaga caatttataa ttaaacaatt ttctatattg ttctgctgtt tcttttttta
      601 cttttaaatt aatttctttc aatctatctt gcatctcaat atttaaaatt gagcccactt
      661 gcatattttt ttcttctgct atcttaatgc aatcttcaaa tcttttttta atttctctgc
      721 tgtctttaac agtcattttc tttattttaa attccatagt cacctcattt tttttatagt
      781 tttttttgtt ttaattttac tccgcaggtt tgcctttttt cttcattcca aattaaaaat
      841 tttcaaattt cgcttcgctc aaaatgaaat atttttaaaa tgtcattacg aaaaaaagca
      901 aaggggtttt gtgctcttcg cacacttttc ctttcaaaat ttcgctgacg ctcatttttc
      961 aagtaaaaga caacccccca ttattgttat aacaaaaata acaataatgg caagtcaaaa
     1021 gaaaaaatag atcgcagatt ttactagatt tttcatcaac ttacacacag atcaatcgct
     1081 caaaaaacaa aattaaaatt ttattttttt cacgattcgc cgcaaaaaaa ttatagtctt
     1141 ctttttttac ttggctgtat tttttgttct tcttcaactt gttcaatcat tattttagat
     1201 atttcttttt cttctttttt tattctttct tcttcaaata aaaaatctaa tgtctcataa
     1261 ttatccggaa aagcaaaaac tttaaatctc gaaaattctt catttgcaca accataacat
     1321 tttaaatatg cagttttgaa attgtcattc ctaaataatt tgtctttaat atgtgacata
     1381 gaaacaagtt cggaataaga gttttctaaa ctcattttgt ttgaagcatt tctgctttca
     1441 cttaatttcc tcaactcttt tttttcgtct aaaatcatgt ttgaaaaatt atttagaatg
     1501 tatctgttat ctacatttct ttctaaaatc tcagtacata tatgagaaaa aaaatgaaaa
     1561 gtcgtttttt ctatattgta atacttcact aaatcaataa ctattttcga aacaatttct
     1621 cttttaattc ttttttttaa tgctgttaaa tgtgcttttc ttctggagtc ttgagatttt
     1681 ctttctttct cactcatatc gctttttaat ttgaaatcac aatatatttt taatatttca
     1741 tatctcaaat cagcaagatc aaatatgttt ttattactgg cgtattcttt aaatcgctct
     1801 ctcaattcac tatttttttt tgatattgtc atatattaag ccctctttat tttgctaata
     1861 catatatgct taatatatta ttttaatatt gtcaaattta ataatttaac ttctccctct
     1921 gctcaaactc tgcatttata taattaatat aaaagataag gctttgttgt tcataatatt
     1981 cagtagagca gattgtatta aatcggtgtt caaactctct aaactcacta ttacacttaa
     2041 aaataaattt ttctaattct tctcgtgtat gtttattata aatttcacgg atcacttttc
     2101 tttctaatgc taaaaaatat ctatctatca atttttctat ttccataaat tttccttttt
     2161 taattatatt tagaatttaa atagcaaatt gcaaaaaaaa atcaaatcgc caattttttc
     2221 ttcgaactct atttttttat tctttagtat tggttttcta tctttctaca aagaagcatt
     2281 gctttttctc tataacagat ctgtattgtt ttgctgtggc ttcgctaaga cctttgtgtt
     2341 cagcccttta cagggcttca cccaaaggtc tcccctagta aaaattttct ggatctaccc
     2401 taaaaatcag gtaattagaa aattttcact aagggcgatt tgtgtgattg caaagttttg
     2461 gacttcacca caaagttcta gactattgag cttaacaacc gtttccagtc gttggcaccc
     2521 tcttacaccc tctagataca gttcatagcc ttttacttgc gacagctaac cccattttaa
     2581 acaaccattt ggctgttatg gctgggcttt ctcatagcct accaatcaat cactgttcac
     2641 caaaaaaacc ttagaactcg cctcccaggt cggcaattcg taagcaccct tctattgcca
     2701 acaaccaacc gaaaccggac cctcttgatt ccacagtttc aggcacgaca caatatgcgc
     2761 atttggttgc ttaaggattt gaaaaactag ggatttttga agagacttgt aacaagagag
     2821 gtttgacaat tgcgatttta taagttagat tttcacttac atatcgtaat ccagtgcctg
     2881 atatcacttg attacaagac tttcagaagt ccccacttct ttaagtccct caaaaccagc
     2941 ttaggctggt tttgttgtat ctggactcag ttataatcta tctttctgtt atgtcaacac
     3001 ttcttcaaaa aatttgattg ctcaaaaatc aaaattctca ctcaatgaga ctcgttgtgt
     3061 catttttaac agcaaaaatg acacttcaaa tgccaataac tgacagttaa ttgatcaaaa
     3121 aaccaccaac gaaaagttac cggggtcccg ttccgggcac tactcgcgcg cgtgccgcct
     3181 gctaacacat atattgatta gttaattaca ccgaccacac aacgaaccac accgatctgt
     3241 ggttctgcta atacacatat gcaaccagcc ggcacagatc tctgctggca agacgcgcgc
     3301 gcgaggctaa cacacatatg agggatcagc attgctgacc cctggtaact ctctaaaatc
     3361 gttttaaggc tcgtttacga gtaggagtag ggtttacccc tcctgggttg gttttcttct
     3421 caaatcgctt tgtattgctt ttaatcgcgt ttgagtgctc aacacctgcg atttttggac
     3481 cacctttgcc agatcttgca tagccgtaat tttccagaga tctagttact ctgtgaccca
     3541 aaaatgcagc tattgtttct tctgtaaaac cctctttttt taattcgctc gctgctctgt
     3601 gtcttaatgt ataaagtgaa aaacctttat ggttgatata caaatattgt ctcaatgaat
     3661 catactcttg ctttgagcat ttatattcta atttattatt ttgctttatt ttggacataa
     3721 tcaat
//
