LOCUS       NZ_CP048394             3544 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Klebsiella pneumoniae strain S01 plasmid pKPS01_p5, complete
            sequence.
ACCESSION   NZ_CP048394
VERSION     NZ_CP048394.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13968567
            Assembly: GCF_023612215.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Zhu,Y., Lu,J., Zhao,J., Velkov,T. and Li,J.
  TITLE     Whole genome sequencing and genome-scale metabolic modelling of
            NDM-producing MDR Klebsiella pneumoniae S01
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Zhu,Y., Lu,J., Zhao,J., Velkov,T. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) Faculty of Medicine, Nursing and Health
            Sciences, Monash University, 19 Innovation Walk, Clayton, VIC 3800,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Assembly Name                     :: FADDI_Monash_KPS01_1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 320.0x
            Sequencing Technology             :: PacBio RSII; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023612215.1-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 03:47:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,510
            CDSs (total)                      :: 5,386
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 55 of 141
            Pseudo Genes (incomplete)         :: 91 of 141
            Pseudo Genes (internal stop)      :: 17 of 141
            Pseudo Genes (multiple problems)  :: 18 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048394.1.
            Bacteria and source DNA available from Laboratory of Antimicrobial
            Systems Pharmacology in Biomedicine Discovery Institute, 19
            Innovation Walk , Monash University, Melbourne, Australia.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="S01"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPS01_p5"
                     /geo_loc_name="Australia: Melbourne"
                     /collection_date="2016-06"
     gene            complement(join(3131..3544,1..63))
                     /locus_tag="GZH33_RS27430"
                     /old_locus_tag="GZH33_27940"
     CDS             complement(join(3131..3544,1..63))
                     /locus_tag="GZH33_RS27430"
                     /old_locus_tag="GZH33_27940"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024243917.1"
                     /translation="MDKSAYVRSGEQTVAGISFEMMNSAAYDTLGLNTSNIELLSFAIY
                     SYHAFRHYQPDLAKEICDSIAKWIVNSHGEHNANERKAAIKKYYPILARGVADAYENNQ
                     TMTTPPHIALGFDICDIIGNGYTPSRCNVIELGLIVKQFLDKGHEKFKEHYLKE"
                     /name="hypothetical protein"
     gene            920..1240
                     /locus_tag="GZH33_RS27435"
                     /old_locus_tag="GZH33_27945"
     CDS             920..1240
                     /locus_tag="GZH33_RS27435"
                     /old_locus_tag="GZH33_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024243919.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLETGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1449..>1880
                     /locus_tag="GZH33_RS27440"
                     /pseudo=""
     CDS             1449..>1880
                     /locus_tag="GZH33_RS27440"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1918..2403
                     /locus_tag="GZH33_RS27445"
                     /old_locus_tag="GZH33_27955"
     CDS             1918..2403
                     /locus_tag="GZH33_RS27445"
                     /old_locus_tag="GZH33_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000096578.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            2407..2619
                     /locus_tag="GZH33_RS27450"
                     /old_locus_tag="GZH33_27960"
     CDS             2407..2619
                     /locus_tag="GZH33_RS27450"
                     /old_locus_tag="GZH33_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          729..813
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.674"
                     /coverage="100.0"
                     /evalue="6.569999999999999e-34"
     Typing          1231..2735
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.418"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 catttcgaat gagattccag ctacagtttg ttcaccagat ctcacatacg ctgatttatc
       61 catcttttta caccataaaa taaatattaa gcataaaaat actaatgccc ccgtggtggg
      121 tataccaaaa aacggggagc ggagcgacca gcgtctggcc gttctatgtt gttctgattg
      181 tgtgggtgtt gtgccagact gggcaatcgc atttcaccgc aactcctgcc ttgaatctca
      241 ctttatcagg tgcttgttat cgtacagcga gacggggtag agcttaactc tttcgcggct
      301 aagttacgtt tccaccgttt gcctgtttat cctgccggag tccggggcgt agacctgagt
      361 ttcaagggtg cagatatgat tttaatgtcg ttgaatggta ttaagctggt agtgacaagg
      421 ctagccagcc ctgctaggat tggattgcta cgtctgatcc tggcaggtat ctgcgaagaa
      481 ttagagtacc gaagccctgt ggttctgtca cggggctttt gtacatctgt agcctgatct
      541 ttcaacaagc tccccgtcac acctcacttc atgtatgtga aaaaatttgt agtgcccgcc
      601 gcagtctcac gtccggagcg tagcgaccga gtgagcgagg aggcgtcatt tcatctgtcc
      661 gtgcatggta ttgcggacgc tcctgagcca catatcacac ctgccacatg atgcactttc
      721 atgattaccg tacccatgcc aacatagtaa gccagtatac actccgctag cgctacgtga
      781 ctggttcagg gctgcgcccc gaaacccgct aaaagcgact gacgcgcctg cggcttgtcc
      841 aacaccgtgc cgcgcgggaa aaatttcccg cccgtcccgg tgttatcagg aggctactct
      901 ggcagataaa cgcaacaaga tgctcacgat gtgggtgacg gaagacgaac accggcggct
      961 gctcgagcgg tgcgacggca ggcagctggc ggcgtggatg cgtcagacct gcctcgatga
     1021 gaagccggca cgtgccggca gactgccgtc gatatcgccg gcgctgctgc gccagcttgc
     1081 cggtatgggt aacaacctga accagatcgc ccggaaagtg aatgccggtg gtgccggtca
     1141 tgatcgggtg cagatcgtgg ccgccctgat ggcaatcgac gcgggactgg agcggctgcg
     1201 gcatgcggtg ctggaaacag ggggggatga tgatcgttaa attccatccc cgggggcgtg
     1261 gtggtggtgc cggtccggtg gattacctgc tgggaaaaga ccggcagcgc gagggggcga
     1321 ccgtgctgca gggaaagccg gaggaagtcc gggagcttat cgatgcctcg ccgtatgcga
     1381 aaaagtacac ctccggtgtg ctgtcgttcg cggaggctga actgccgccc gggcagcggg
     1441 agcagatcat ggccagcttt gagcgggttc tgatgcccgg gctcgataaa gaccagtaca
     1501 gcattctgtg ggtggagcac accgacaagg ggcggctgga gctgaatttc ctgatcccca
     1561 acactgaact gctgaccggg aaacgcctgc agccgtacta cgatcgcgcc gaccggccgc
     1621 gcatcgatgc ctggcagacc gtggtgaacg gcagactggg gctgcatgat ccaaacgccc
     1681 cggagaaccg gcggttactg gtcacgccgt ccgcactgcc ggaaacgaag ctggaagccg
     1741 ctcaggcgat tacacggggg ttgctggcct tagcctcatc cggggagcta aaaacgcgcg
     1801 aggacgtcac aggggcgctc acagcggcag gttttgaggt ggtccgcacc acaaaaaaca
     1861 gtatcagcat tgccgacccg gacggggggc gaaacatccg actgaaggga gccatttatg
     1921 agcagtcttt tagcgctggc gaaggacttg gagcagcaat cgaaagcgca gcagcagagt
     1981 accggcgaga tgctgaaagc cgcattcagc gagcacgaga agtctgtaaa agcggaactg
     2041 agcgcaagcg cgaagagaat cagcgacgcc atcagcgccc acgagcaggg tatgaccgcg
     2101 gcgatgcagt cgaaccggct gagcgtgctg cgtatggtcg gccggacgtg gctgaccatc
     2161 accctggtct cggtactgct gatcgccacg agcgggagca ttctgtggtg gcaggggcag
     2221 cagatcaccg ggaattacca gactatccgg gcgcaggagc ggacgcaggc catgctgtcg
     2281 gagaagaacc acggcgtgca gctgtcgctc tgcggggaga agaaactgtc ctgcgtcaaa
     2341 gtgaacccga aagcgggagc ttatggggag gaagggaact ggatggtact ggagaggaaa
     2401 tagcccatga cggaactgga aaaacagttg ctgaccgcat tagagcagtt acatcaggac
     2461 tactcgcaaa ggctggacga gtgggagagc gccttcgcgg aatggcagaa gatgtctggt
     2521 cttatgcaac gggagaacgc ggcgctgaac gagcgcgtca cgcgcttgag tcagcaggtg
     2581 gcgaacttga gcaggcagtt acagcgtttg agccagtagt gcagcggcat gaaatggcgg
     2641 tggccgccga acgggcatac gagcagaaac agcatgaaaa agagctggct gaaacccgtt
     2701 cccggcagcg gagctataac ggtccgacgc tgggctgact gcgcgcatcc agtgacaaac
     2761 cccaagaaga agggctcata aatttcccgc taaatacggt cgtgatgttt ccatcacagc
     2821 gtatacaacg gagggattat gagactgttt aacgtgattg agacggtgct gaactgcctg
     2881 tacatggcgc tggagatctg ggactggctg gagaagcatt ttcctgtttt taaggcgttc
     2941 atccggattc tggcctgaaa tcagaccgta tgcatctggc tgcatgacca tgcagccggg
     3001 taaaatttat ctgtcacgaa cggggcggtg tatcgggtgg tttgttgccg gttttacctg
     3061 actgccgccc cgtttgtggc gaacctgtcc ggagcggtgc gggcaacgga tgttatggta
     3121 aatacaggca ttattccttc agataatgtt ccttaaattt ctcatgtcct ttatctagaa
     3181 actgcttaac gatgaggcct aactcaatca cgttacaacg agaaggtgtg tagccgttac
     3241 caatgatgtc acatatatca aaaccgagag caatatgagg tggggtagtc attgtttggt
     3301 tattctcgta agcgtcggcg actcctctgg ctaagatagg gtaatatttt ttgatagcgg
     3361 cttttctctc attagcgtta tgttctccat gagaatttac tatccatttg gcaatgctat
     3421 cgcaaatttc tttagctagg tctggttggt aatgtcggaa ggcgtgataa ctgtaaatgg
     3481 cgaagctaag caattctata ttcgaagtgt ttagacctag agtgtcgtag gctgccgagt
     3541 tcat
//
