LOCUS       NZ_CP048378             2088 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain 38 plasmid p38_B, complete sequence.
ACCESSION   NZ_CP048378
VERSION     NZ_CP048378.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951915
            Assembly: GCF_010365545.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: 38.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010365545.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:30:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,700
            CDSs (total)                      :: 4,583
            Genes (coding)                    :: 4,332
            CDSs (with protein)               :: 4,332
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 96 of 251
            Pseudo Genes (incomplete)         :: 155 of 251
            Pseudo Genes (internal stop)      :: 49 of 251
            Pseudo Genes (multiple problems)  :: 42 of 251
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048378.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="38"
                     /isolation_source="environment"
                     /db_xref="taxon:562"
                     /plasmid="p38_B"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            271..1236
                     /locus_tag="GZS06_RS23190"
                     /old_locus_tag="GZS06_23195"
     CDS             271..1236
                     /locus_tag="GZS06_RS23190"
                     /old_locus_tag="GZS06_23195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_039064478.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLAIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            1240..1506
                     /locus_tag="GZS06_RS23195"
                     /old_locus_tag="GZS06_23200"
     CDS             1240..1506
                     /locus_tag="GZS06_RS23195"
                     /old_locus_tag="GZS06_23200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          665..896
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 tgtatttata cttaagggat aaatggcggg tatgaaatag tggtttagcc cagtaatgac
       61 gaggctttga gtgggttttg acaggtcaaa gaaaatggag cagaattgag gcgtttttaa
      121 tcggcgttgg ggagtgcgtc aacactcccc aacatttcga atgtgtcacc tcagcggcaa
      181 actctggtga catgtactgg ctcgcaatgc acaggtacgt gatgaatata ccacatcaaa
      241 tcacagcctg cccagatcgg agcaggctta atgtcagaag ataaattcct ttcggactac
      301 agcccccgtg atgcagtttg ggatacccag cgcacgctta ccgattctgt cgggggtatc
      361 taccagactg ctgctgaatt cgagcgctat gcactccgta tggcctcctg tagcggtttg
      421 ttacgttttg gttggtctac catcatggaa accggagaaa cgcgcctacg gcttcgtagt
      481 gcgcaatttt gccgtgtccg tcattgccct gtctgccagt ggagaagaac cctcatgtgg
      541 caagcccgtt tttatcaggc tctaccgaaa atcgttgtgg attacccgtc ttcccgatgg
      601 ttgtttctga cgttaactgt caggaactgc gagataggtg aacttggaac agtccttaca
      661 gcaatgaatg cggcgtttaa gcgaatggaa aagcgaaagg agctatcacc tgttcagggg
      721 tggatcaggg ctacggaggt gacgcgaggt aaggatggca gcgcacatcc gcattttcac
      781 tgtctgctga tggtgcaacc ttcttggttt aaagggaaga actacgttaa gcacgaacgt
      841 tgggtagaac tctggcgcga ttgcttgcgg gtgaactatg agccgaatat cgatattcgg
      901 gcagtaaaaa ctaagacagg tgaggttgtg gccaacgttg ccgagcaact gcaaagcgcg
      961 gttgctgaaa cgctgaaata ctccgttaaa ccggaagata tggcaaacga tcctgagtgg
     1021 tttcttgagc tgacgcggca gcttcacaag cgccgtttta tctcgaccgg tggagcgcta
     1081 aaaaacgtcc tccagttgga tcgagaaacc aatgaggatc ttgccattgc cgacgatgta
     1141 ggggatggca ctgatgacgg gaagcggacg gcgtttgtct gggattcagg taaacggcgt
     1201 tacaaacgcg cccctgagaa ggataaatcg gattaacgta tgaatattaa tattgaatac
     1261 ctgaatggaa ataagactat tggtttattt tttttaagaa gtgaagcggt gattcctgac
     1321 aggtttaaaa accttatttt gcttattgat ggattaagtt ttggcacatt tggttttcat
     1381 ccgcacgaag gttttgagga tgaattaatt ttatatattc agaaaacaaa cgagagggta
     1441 aaaactcttt ttgtgaaaat agatttaaac ggtatcaagt ttggattttt aagaacgcat
     1501 tcttagttct ggaaaagagc cagcggcagg ctgaggtgat aggtacgaga ttgcatgcaa
     1561 tctctagtgc tctgtctatc ctgcattatc ctcagcatta tcctcagcct tgccaactcg
     1621 acaccaatgc aggatagaca atccgatgtc aaatgttaac actctgcgag tggtacattt
     1681 tccccggatt atcgtcctga gcctgccgct ggctctcttt ctaccgcctc gctttgctcg
     1741 ttgctcaacg cctcacagac acggattaaa atccgcatcc gttcaccgtt ttttaaagtc
     1801 cgttaaaagc atgatgccat ctccgagagt taatctcgtc aaatgctaaa tcgtgggggt
     1861 cccctttggg gttccgattt agtgattgac gacaccaccg attaaaaaac ttatgcgggg
     1921 tggatggttt cacgaagtga ggccatccac ctgtaagaca gggttttgtt tttatcccct
     1981 gttttggtga tcgggtgtgt ggaaaaggtt ggggtaagcc gttcggggtg cttgttttgg
     2041 ggggttaaaa ttgtggttat tttttgcgca attctcgcgc gtgatcct
//
