LOCUS       NZ_CP048374            10494 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain 163 plasmid pC-F-163_C, complete sequence.
ACCESSION   NZ_CP048374
VERSION     NZ_CP048374.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951917
            Assembly: GCF_010365505.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10494)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10494)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: C-F-163.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010365505.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:31:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,875
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,502
            CDSs (with protein)               :: 4,502
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 86 of 254
            Pseudo Genes (incomplete)         :: 165 of 254
            Pseudo Genes (internal stop)      :: 58 of 254
            Pseudo Genes (multiple problems)  :: 50 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048374.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="163"
                     /isolation_source="environment"
                     /db_xref="taxon:562"
                     /plasmid="pC-F-163_C"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
                     /note="This genome was originally submitted with the wrong
                     strain name and BioSample. It was corrected on March 18,
                     2020."
     gene            complement(<3..869)
                     /gene="intI1"
                     /locus_tag="GZS08_RS24130"
                     /old_locus_tag="GZS07_23875"
                     /pseudo=""
     CDS             complement(<3..869)
                     /gene="intI1"
                     /locus_tag="GZS08_RS24130"
                     /old_locus_tag="GZS07_23875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /name="intI1"
     gene            1014..1511
                     /gene="dfrA12"
                     /locus_tag="GZS08_RS23880"
                     /old_locus_tag="GZS07_23880"
                     /old_locus_tag="GZS07_RS23880"
     ARG             1015..1508
                     /name="dfrA12"
                     /gene="dfrA12"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA12"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1623..1913
                     /locus_tag="GZS08_RS23885"
                     /old_locus_tag="GZS07_23885"
                     /old_locus_tag="GZS07_RS23885"
     CDS             1623..1913
                     /locus_tag="GZS08_RS23885"
                     /old_locus_tag="GZS07_23885"
                     /old_locus_tag="GZS07_RS23885"
                     /inference="COORDINATES: protein motif:HMM:NF017990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1010 domain-containing protein"
                     /protein_id="WP_001336345.1"
                     /translation="MFIQTAFSFSGVIQCLFCLFSGLRLHGLRRFSVFLASSPCVASAS
                     SYRFCSAVPPRWRSVFSRLAPVAKFKLSVLASGSNISVKPTRILRSAYLAR"
                     /name="DUF1010 domain-containing protein"
     gene            1919..2757
                     /locus_tag="GZS08_RS23890"
                     /old_locus_tag="GZS07_23890"
                     /old_locus_tag="GZS07_RS23890"
                     /pseudo=""
     CDS             1919..2757
                     /locus_tag="GZS08_RS23890"
                     /old_locus_tag="GZS07_23890"
                     /old_locus_tag="GZS07_RS23890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /name="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
     gene            2874..3221
                     /locus_tag="GZS08_RS23895"
                     /old_locus_tag="GZS07_23895"
                     /old_locus_tag="GZS07_RS23895"
     CDS             2874..3221
                     /locus_tag="GZS08_RS23895"
                     /old_locus_tag="GZS07_23895"
                     /old_locus_tag="GZS07_RS23895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="WP_000679427.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAFY
                     FLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLARSP
                     SWKSLRRPTPW"
                     /name="quaternary ammonium compound efflux SMR transporter
                     QacE delta 1"
     gene            3215..4054
                     /gene="sul1"
                     /locus_tag="GZS08_RS23900"
                     /old_locus_tag="GZS07_23900"
                     /old_locus_tag="GZS07_RS23900"
     ARG             3216..4051
                     /name="sul1"
                     /gene="sul1"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4459..6000
                     /locus_tag="GZS08_RS23905"
                     /old_locus_tag="GZS07_23905"
                     /old_locus_tag="GZS07_RS23905"
     CDS             4459..6000
                     /locus_tag="GZS08_RS23905"
                     /old_locus_tag="GZS07_23905"
                     /old_locus_tag="GZS07_RS23905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050481.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISCR1 family transposase"
                     /protein_id="WP_000050481.1"
                     /translation="MSLARNATASQSPTQTNGYERHQPDQTLLYQLVEQHYPAFKASLE
                     AQGQHLPRYIQQEFNDLLQCGRLEYGFMRVRCEDCHHERLVAFSCKRRGFCPSCGARRM
                     AESAALLIDEVFPKEPIRQWVLSFPFQLRFLLARHPQLMGQVLSIVYRTLSTHLIKKAG
                     YTKASAQTGSVTLIQRFGSALNLNVHYHMLFLDGVYAEDDYGKQRFHRVKAPTYDELNT
                     LAHTLSHRIARCMEKRGILERDAENTWLTLEEGEDDTLTQLHGASVTYRIAVGPQQGRK
                     VFTLQTLPGREDKADSSSRVANHAGFSLHAGVMAEAHQRDKLERLCRYISRPAVSEKRL
                     ALTANGQVRYELKTPYRNGTTHVIFEPLDFIAKLAALVPKPRVNLTRFHGVFAPNSKHR
                     VQVTPAKRGKKPDKSEGLDTNWRDKSPAERHRAMTWMQRLKRVFNIDIEVCEHCGGHVK
                     VIASIEDPKVIEQILKHLKQKTAKANAAKQRELPPERAPPLTPSLFDPSQSRLFD"
                     /name="IS91-like element ISCR1 family transposase"
     gene            6313..>6687
                     /locus_tag="GZS08_RS24485"
                     /pseudo=""
     CDS             6313..>6687
                     /locus_tag="GZS08_RS24485"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /name="protein-disulfide reductase DsbD domain-containing
                     protein"
     gene            <6787..7344
                     /locus_tag="GZS08_RS24490"
                     /pseudo=""
     CDS             <6787..7344
                     /locus_tag="GZS08_RS24490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399920.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation (TAT) pathway signal
                     sequence domain protein"
                     /name="twin-arginine translocation (TAT) pathway signal
                     sequence domain protein"
     gene            complement(7355..7993)
                     /locus_tag="GZS08_RS23915"
                     /old_locus_tag="GZS07_23915"
                     /old_locus_tag="GZS07_RS23915"
     CDS             complement(7355..7993)
                     /locus_tag="GZS08_RS23915"
                     /old_locus_tag="GZS07_23915"
                     /old_locus_tag="GZS07_RS23915"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201168.1"
                     /GO_function="GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA]"
                     /GO_process="GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="WP_004201168.1"
                     /translation="MPAKIKICGISTPEALDATIAARADYAGLVFYPASPRAVTSNVAG
                     ALTSRAAGQIAMVGLFVDADDAVIADALVAAKLNALQLHGSESPERVAQLRARFGKPVW
                     KALPVASASDVARAAAYAGAADLILFDAKTPKGALPGGMGLAFDWSLLAGYRGALPWGL
                     AGGLNPTNVAEAIARTGAPLVDTSSGVESAPGVKDTDKITNFAFAVRLA"
                     /name="phosphoribosylanthranilate isomerase"
     gene            complement(7998..8363)
                     /gene="ble"
                     /locus_tag="GZS08_RS23920"
                     /old_locus_tag="GZS07_23920"
                     /old_locus_tag="GZS07_RS23920"
     ARG             complement(8002..8363)
                     /name="ble"
                     /gene="ble"
                     /gene_name="bleomycin binding protein Ble-MBL"
                     /tool="amrfinderplus"
                     /drug_class="BLEOMYCIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(8367..9179)
                     /locus_tag="GZS08_RS23925"
                     /old_locus_tag="GZS07_23925"
                     /old_locus_tag="GZS07_RS23925"
     CDS             complement(8367..9179)
                     /locus_tag="GZS08_RS23925"
                     /old_locus_tag="GZS07_23925"
                     /old_locus_tag="GZS07_RS23925"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase NDM-5"
                     /protein_id="WP_023408309.1"
                     /translation="MELPNIMHPVAKLSTALAAALMLSGCMPGEIRPTIGQQMETGDQR
                     FGDLVFRQLAPNVWQHTSYLDMPGFGAVASNGLIVRDGGRVLLVDTAWTDDQTAQILNW
                     IKQEINLPVALAVVTHAHQDKMGGMDALHAAGIATYANALSNQLAPQEGLVAAQHSLTF
                     AANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLG
                     DADTEHYAASARAFGAAFPKASMIVMSHSAPDSRAAITHTARMADKLR"
                     /name="subclass B1 metallo-beta-lactamase NDM-5"
     gene            complement(9280..>9573)
                     /locus_tag="GZS08_RS23930"
                     /old_locus_tag="GZS07_23930"
                     /old_locus_tag="GZS07_RS23930"
                     /pseudo=""
     CDS             complement(9280..>9573)
                     /locus_tag="GZS08_RS23930"
                     /old_locus_tag="GZS07_23930"
                     /old_locus_tag="GZS07_RS23930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611607.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /name="IS30 family transposase"
     gene            9738..10442
                     /locus_tag="GZS08_RS23935"
                     /old_locus_tag="GZS07_23935"
                     /old_locus_tag="GZS07_RS23935"
     CDS             9738..10442
                     /locus_tag="GZS08_RS23935"
                     /old_locus_tag="GZS07_23935"
                     /old_locus_tag="GZS07_RS23935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     ARG             2875..3218
                     /name="qacEdelta1"
                     /gene="qacEdelta1"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1920..2707
                     /name="aadA2"
                     /gene="aadA2"
                     /gene_name="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA2"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(8371..9179)
                     /name="blaNDM-5"
                     /gene="blaNDM-5"
                     /gene_name="subclass B1 metallo-beta-lactamase NDM-5"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1932..2710
                     /name="aadA2"
                     /gene="aadA2"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(7999..8363)
                     /name="BRP(MBL)"
                     /gene="BRP(MBL)"
                     /gene_name="Bleomycin resistant protein"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 ctgcggagca aggccgtcgc gaacgagtgg cggagggtgt gcggtgtggc gggcttcgtg
       61 atgcctgctt gttctacggc acgtttgaag gcgcgctgaa aggtctggtc atacatgtga
      121 tggcgacgca cgacaccgct ccgtggatcg gtcgaatgcg tgtgctgcgc aaaaacccag
      181 aaccacggcc aggaatgccc ggcgcgcgga tacttccgct caagggcgtc gggaagcgca
      241 acgccgctgc ggccctcggc ctggtccttc agccaccatg cccgtgcacg cgacagctgc
      301 tcgcgcaggc tgggtgccaa gctctcgggt aacatcaagg cccgatcctt ggagcccttg
      361 ccctcccgca cgatgatcgt gccgtgatcg aaatccagat ccttgacccg cagttgcaaa
      421 ccctcactga tccgcatgcc cgttccatac agaagctggg cgaacaaacg atgctcgcct
      481 tccagaaaac cgaggatgcg aaccacttca tccggggtca gcaccaccgg caagcgccgc
      541 gacggccgag gtcttccgat ctcctgaagc cagggcagat ccgtgcacag caccttgccg
      601 tagaagaaca gcaaggccgc caatgcctga cgatgcgtgg agaccgaaac cttgcgctcg
      661 ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc
      721 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttggtaag
      781 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      841 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tgtacagtct
      901 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
      961 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttagc catatgaact
     1021 cggaatcagt acgcatttat ctcgttgctg cgatgggagc caatcgggtt attggcaatg
     1081 gtcctaatat cccctggaaa attccgggtg agcagaagat ttttcgcaga ctcactgagg
     1141 gaaaagtcgt tgtcatgggg cgaaagacct ttgagtctat cggcaagcct ctaccgaacc
     1201 gtcacacatt ggtaatctca cgccaagcta actaccgcgc cactggctgc gtagttgttt
     1261 caacgctgtc gcacgctatc gctttggcat ccgaactcgg caatgaactc tacgtcgcgg
     1321 gcggagctga gatatacact ctggcactac ctcacgccca cggcgtgttt ctatctgagg
     1381 tacatcaaac cttcgagggt gacgccttct tcccaatgct caacgaaaca gaattcgagc
     1441 ttgtctcaac cgaaaccatt caagctgtaa ttccgtacac ccactccgtt tatgcgcgtc
     1501 gaaacggcta accattccgt caacgggacg ccaaaatgct gcgcattttg gttccctccg
     1561 ctgcgctccg gctctcgtta cgtccaacgt tagcaccact gaaacccagc tttatttagc
     1621 tcatgtttat tcaaacggca tttagctttt caggcgttat tcagtgcctg ttttgccttt
     1681 tttccgggct tcgcctgcat gggctgcgca ggttttcagt ctttttggcc tctagccctt
     1741 gcgtagcaag cgcaagcagc tatcgttttt gcagtgctgt gccgcctcgg tggcgcagcg
     1801 ttttttcacg gttagcgccc gtcgccaaat tcaagttatc cgttttggct tctggttcta
     1861 acatttcggt caagccgacc cgcattctgc ggtcggctta cctcgcccgt tagacatcat
     1921 gagggaagcg gtgaccatcg aaatttcgaa ccaactatca gaggtgctaa gcgtcattga
     1981 gcgccatctg gaatcaacgt tgctggccgt gcatttgtac ggctccgcag tggatggcgg
     2041 cctgaagcca tacagcgata ttgatttgtt ggttactgtg gccgtaaagc ttgatgaaac
     2101 gacgcggcga gcattgctca atgatcttat ggaggcttcg gctttccctg gcgagagcga
     2161 gacgctccgc gctatagaag tcacccttgt cgtgcatgac gacatcatcc cgtggcgtta
     2221 tccggctaag cgcgagctgc aatttggaga atggcagcgc aatgacattc ttgcgggtat
     2281 cttcgagcca gccatgatcg acattgatct agctatcctg cttacaaaag caagagaaca
     2341 tagcgttgcc ttggtaggtc cggcagcgga ggaattcttt gacccggttc ctgaacagga
     2401 tctattcgag gcgctgaggg aaaccttgaa gctatggaac tcgcagcccg actgggccgg
     2461 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcaa taaccggcaa
     2521 aatcgcgccg aaggatgtcg ctgccgactg ggcaataaaa cgcctacctg cccagtatca
     2581 gcccgtctta cttgaagcta agcaagctta tctgggacaa aaagaagatc acttggcctc
     2641 acgcgcagat cacttggaag aatttattcg ctttgtgaaa ggcgagatca tcaagtcagt
     2701 tggtaaatga tgtctaacaa ttcgttcaag ccgaccgcgc tacgcgcggc ggcttaactc
     2761 cggcgttaga tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct
     2821 tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgaaag
     2881 gctggctttt tcttgttatc gcaatagttg gcgaagtaat cgcaacatcc gcattaaaat
     2941 ctagcgaggg ctttactaag cttgcccctt ccgccgttgt cataatcggt tatggcatcg
     3001 cattttattt tctttctctg gttctgaaat ccatccctgt cggtgttgct tatgcagtct
     3061 ggtcgggact cggcgtcgtc ataattacag ccattgcctg gttgcttcat gggcaaaagc
     3121 ttgatgcgtg gggctttgta ggtatggggc tcataattgc tgcctttttg ctcgcccgat
     3181 ccccatcgtg gaagtcgctg cggaggccga cgccatggtg acggtgttcg gcattctgaa
     3241 tctcaccgag gactccttct tcgatgagag ccggcggcta gaccccgccg gcgctgtcac
     3301 cgcggcgatc gaaatgctgc gagtcggatc agacgtcgtg gatgtcggac cggccgccag
     3361 ccatccggac gcgaggcctg tatcgccggc cgatgagatc agacgtattg cgccgctctt
     3421 agacgccctg tccgatcaga tgcaccgtgt ttcaatcgac agcttccaac cggaaaccca
     3481 gcgctatgcg ctcaagcgcg gcgtgggcta cctgaacgat atccaaggat ttcctgaccc
     3541 tgcgctctat cccgatattg ctgaggcgga ctgcaggctg gtggttatgc actcagcgca
     3601 gcgggatggc atcgccaccc gcaccggtca ccttcgaccc gaagacgcgc tcgacgagat
     3661 tgtgcggttc ttcgaggcgc gggtttccgc cttgcgacgg agcggggtcg ctgccgaccg
     3721 gctcatcctc gatccgggga tgggattttt cttgagcccc gcaccggaaa catcgctgca
     3781 cgtgctgtcg aaccttcaaa agctgaagtc ggcgttgggg cttccgctat tggtctcggt
     3841 gtcgcggaaa tccttcttgg gcgccaccgt tggccttcct gtaaaggatc tgggtccagc
     3901 gagccttgcg gcggaacttc acgcgatcgg caatggcgct gactacgtcc gcacccacgc
     3961 gcctggagat ctgcgaagcg caatcacctt ctcggaaacc ctcgcgaaat ttcgcagtcg
     4021 cgacgccaga gaccgagggt tagatcatgc ctagcattca ccttccggcc gcccgctaaa
     4081 tatctccttt tgggttgtta ataaaacatc caataagttg actgtgcgtg aaaaagaaag
     4141 ttttgtgtga tggcgttgaa gatcgcaccg ttaagctctt atgtgggatg gtgcagagct
     4201 cgacgactac cgataaaacg caaccgccgc aaacagacaa gaaaaagccc caactgataa
     4261 cagttggggc ttcagtattg tgattggtgg agcaatagca ccctgaaccc aaaaccttct
     4321 cgctcaaccg gtagtggctg ataacaactc gtgagggcta ttgcgggtta agcatttagc
     4381 gatgtctagg gccagactgg acgtctgaac gcaagccgct gatactgtac ataaccacag
     4441 tatcagcgga ggatacccat gtcgctggca aggaacgcca cggcgagtca atcgcccact
     4501 caaacaaacg gttacgaacg ccaccaaccc gaccagacgc tgctctacca gctggttgag
     4561 cagcactacc cagccttcaa agcctcactc gaagcccaag gtcaacacct gcctcgctac
     4621 atccaacaag aattcaacga cctcctccaa tgtggccgtc tggagtatgg tttcatgcgg
     4681 gttcgctgcg aggattgtca tcacgagcgt ctggtcgcct tcagctgtaa acgacgcggc
     4741 ttttgcccta gctgcggtgc ccgccggatg gccgagagtg cggcgctgct gatagacgaa
     4801 gtcttcccca aggagcccat tcgccagtgg gtgctcagct ttcctttcca gctacgcttt
     4861 ttgctggctc gccatcccca gctgatgggc caggtcttga gtatcgtcta tcgtacactc
     4921 tcaactcatc tgatcaaaaa agccggttac accaaagcct ctgcacaaac tggctcagtg
     4981 actcttatcc aacgctttgg ctccgcgcta aatctcaatg tccactacca catgctgttt
     5041 ctcgatggtg tctatgccga agatgactat ggcaagcaac gcttccatcg tgtcaaggca
     5101 cccacttacg atgagctgaa tacgctcgct cacaccctca gccatcgcat cgctcgctgc
     5161 atggaaaagc gtgggatttt ggagcgtgat gccgagaata cgtggttgac actggaagag
     5221 ggcgaagacg atacgctgac tcaattacat ggtgcttcgg ttacgtatcg cattgccgtc
     5281 ggcccccagc aagggcgcaa agtcttcacc ctgcaaacct tgccagggcg tgaggataaa
     5341 gccgactcaa gcagtcgagt agccaaccat gctggtttct cgctacacgc cggtgtgatg
     5401 gccgaagcgc atcagcggga taagcttgag cgcttgtgtc gctacattag tcggccagcg
     5461 gtttcagaaa aacgtctggc attaaccgcc aatgggcagg tgcgttacga gctcaaaact
     5521 ccgtaccgca atggcaccac ccatgtgatc ttcgagccgc tggacttcat cgccaaactc
     5581 gctgcgttgg tacctaagcc gcgagtcaac ctcacacgct tccacggcgt ctttgcaccg
     5641 aacagcaaac accgagttca agtaacaccc gccaagcggg gcaagaagcc cgacaaatcg
     5701 gaaggtctcg atactaactg gcgtgacaag agtcctgcag agcgccaccg cgccatgacc
     5761 tggatgcaac gcctcaagcg agtcttcaat attgatattg aagtctgcga acactgcggc
     5821 ggtcacgtca aagtgattgc cagcatcgaa gatccgaagg tcattgagca gattctcaag
     5881 catctgaaac agaaaacagc caaggcgaat gccgccaagc agcgtgagct gccaccagaa
     5941 cgagcgccgc cactgactcc cagcctgttc gatccatcac agagtcgtct ctttgactga
     6001 cgaccccaaa tccaacactg ctcaacactg ccaactttta aacggggcgg tggggcagtt
     6061 tgtatctctc gagctatcag gctagagatt ttaccgccaa atcgaacctt attagagcgg
     6121 tttaggctgg accggcagtt aaaattgggg cttgagcggt aaacgagtga gggaatttca
     6181 ggtaagatac ttcggatgag gagcaaaaag gtggtttata cttcctatac ccgctcgcgg
     6241 tcgaagccgc gtccggcctg cccgaatacc tgcaatggct ggccgccgag agccgaccgg
     6301 taaactgagc caatgaccgc atccacgatc cgcctgcgcc gctggctggc cgggctcgcc
     6361 ctgctgcttg cgctgcccgc gacctcggcc gtcgcccagg acttcgaact gccgccggtc
     6421 gacgaggtct tcgtcctgtc cgcgcaggcc accgcgccgg accgcatcga ggtgcgctgg
     6481 cggatcgccg acggctatta cctgtaccgg caccgcacct cggtcaaggc cgatgccgcc
     6541 ttcaccggcg cgaccatggc gctgccgaag ggcaaggcct accgcgacga attcttcggc
     6601 gacgtcgaaa cctaccgcaa ggaattgctc ggcaccctca ccggcacgcc cgcggccggc
     6661 gcgagcgcga ccaccctgac cgtgaagtac cagggctgcg ccgatgccgg cgtgtgctac
     6721 ccgccgcaga cccgcaccct gaaggtcgcg ttgccgggcg aagcgggcgc tggcggcttc
     6781 ggctgtaagg cgcgcgggct gcatgattat gtcgtcaaga acggctctgc cgatcatccc
     6841 aacgccgagg tgaaattcgc actgggtgat gtggtcaaca ccatgatcgg ctgcactaat
     6901 ggtgaaacga tcatgctgtg ccacgacacc tcgctgccgc gcccctattc tctcggcttt
     6961 cgggtgcaag gcaccgaggg gctgtggatg gacgtcaaca agtcgatcta tctggagggc
     7021 aagagcccac agccgcaccg ctgggagcct gccgagggct ggtttgcgaa atacgatcac
     7081 ccgctatgga aacgctacgc cgatctggcg gcaggggccg ggcatggcgg gatggactgg
     7141 ttcgtgatcc acgcttttgt cgaggcgctg aaggccaagg ccccgatgcc aatcgacatt
     7201 tacgacgcgc tggcctggag cgcgatcacg cctctgtcgg aacaatcgat tgctgagggc
     7261 aatcgcacgt tagattttcc cgacttcacc cgagggcagt ggcgcacccg caagccgatc
     7321 tttgcgctga acgacgccta ttgatcgacg cgatttaggc caagcgcacc gcaaaggcga
     7381 aattggtaat cttgtcggta tccttgacgc ccggcgcgct ttcgacgccg ctggaggtat
     7441 cgaccagcgg cgctccggtg cgcgcaatcg cctcggcaac attcgtcgga tttagcccgc
     7501 ctgccagccc ccacggcaag gcaccgcgat atccggccag cagcgaccag tcgaacgcca
     7561 accccatgcc gccgggcagc gcgcctttgg gggtcttggc gtcgaacaag atcaagtccg
     7621 ccgccccggc ataggctgcg gcgcgtgcga catcgctggc gctggcgacg ggcagcgcct
     7681 tccacaccgg cttgccaaac cgcgcgcgca actgggccac gcgttcgggc gattccgaac
     7741 cgtgcagctg cagcgcgttc agcttggctg ccaccagtgc gtcggcgatg acagcatcat
     7801 ccgcatcgac gaacaaaccg accatggcga tctggccagc tgcgcgcgat gtcaaagcgc
     7861 ccgcgacatt cgacgtaacc gcacggggcg acgctggata gaacaccaac ccggcatagt
     7921 ccgcccgcgc cgcgatggtc gcatcgagcg cctcgggtgt gctgatcccg caaatcttga
     7981 ttttcgcggg catgcggtca gtcggggttc tggatcagcc gcaccagcgt gcagtcggga
     8041 tcgatcaggt agccgatcct caggccgctc gcctccagtt gcggagcttt gaagcgcggc
     8101 cagccggtgc ttttttcctc ggctcccgcc gcgttcacca atgccaccat ggcatcgaga
     8161 tcatccaacc gcaggcaaca gccgaacgag ctcgtagctg ggtcgaggtc aggatagggg
     8221 aagaattcga gctgcaaacc gccgcgctgc aggatcatcc agccgcgatc cttccaactc
     8281 gtcgcaaagc ccagcttcgc ataaaacgcc tctgtcacat cgaaatcgcg cgatggcaga
     8341 ttgggggtga cgtggtcagc catggctcag cgcagcttgt cggccatgcg ggccgtatga
     8401 gtgattgcgg cgcggctatc gggggcggaa tggctcatca cgatcatgct ggccttgggg
     8461 aacgccgcac caaacgcgcg cgctgacgcg gcgtagtgct cagtgtcggc atcaccgaga
     8521 ttgccgagcg acttggcctt gctgtccttg atcaggcagc caccaaaagc gatgtcggtg
     8581 ccgtcgatcc caacggtgat attgtcactg gtgtggccgg ggccggggta aaataccttg
     8641 agcgggccaa agttgggcgc ggttgctggt tcgacccagc cattggcggc gaaagtcagg
     8701 ctgtgttgcg ccgcaaccag cccctcttgc ggggcaagct ggttcgacaa cgcattggca
     8761 taagtcgcaa tccccgccgc atgcagcgcg tccataccgc ccatcttgtc ctgatgcgcg
     8821 tgagtcacca ccgccagcgc gaccggcagg ttgatctcct gcttgatcca gttgaggatc
     8881 tgggcggtct ggtcatcggt ccaggcggta tcgaccaaca gcacgcggcc gccatccctg
     8941 acgatcaaac cgttggaagc gactgccccg aaacccggca tgtcgagata ggaagtgtgc
     9001 tgccagacat tcggtgcgag ctggcggaaa accagatcgc caaaccgttg gtcgccagtt
     9061 tccatttgct ggccaatcgt cgggcggatt tcaccgggca tgcacccgct cagcatcaat
     9121 gcagcggcta atgcggtgct cagcttcgcg accgggtgca taatattggg caattccatc
     9181 aagttttcct tttattcagc attaaaaacc ccgcaaatgc gaggcctagt aaatagatga
     9241 tcttaatttg gttcactgta gcaaaaatat ggggcgaatt caaacatgag gtgcgacagt
     9301 ttcaaaagcc atatgataat caacaagctg agcaaatttc tctaatggtg taagccaatc
     9361 taacgccttt ctaggacgag tattcagtga catggcaact tgatttaaat aatgctgatc
     9421 tgcctgattt aaatcaatcc ctttaggtaa atattgccta attaaaccat tcatattttc
     9481 gcatgtgcct ttttgccagg gtgaatgtgg gtcacagaaa tatacatcta tgcctaaatc
     9541 ttcttcgagt attttatgtt gagcactttt ctttcaccca atgggtgaaa gctgaatttc
     9601 gaaggaatgc atatttatca aggctttgat tatgcttttt gaagtactga cgtagaatct
     9661 aggtatgagt aaacggcact gttgcaaata gtcggtggtg ataaacttat catccccttt
     9721 tgctgatgga gctgcacatg aacccattca aaggccggca ttttcagcgt gacatcattc
     9781 tgtgggccgt acgctggtac tgcaaatacg gcatcagtta ccgtgagctg caggagatgc
     9841 tggctgaacg cggagtgaat gtcgatcact ccacgattta ccgctgggtt cagcgttatg
     9901 cgcctgaaat ggaaaaacgg ctgcgctggt actggcgtaa cccttccgat ctttgcccgt
     9961 ggcacatgga tgaaacctac gtgaaggtca atggccgctg ggcgtatctg taccgggccg
    10021 tcgacagccg gggccgcact gtcgattttt atctctcctc ccgtcgtaac agcaaagctg
    10081 cataccggtt tctgggtaaa atcctcaaca acgtgaagaa gtggcagatc ccgcgattca
    10141 tcaacacgga taaagcgccc gcctatggtc gcgcgcttgc tctgctcaaa cgcgaaggcc
    10201 ggtgcccgtc tgacgttgaa caccgacaga ttaagtaccg gaacaacgtg attgaatgcg
    10261 atcatggcaa actgaaacgg ataatcggcg ccacgctggg atttaaatcc atgaagacgg
    10321 cttacgccac catcaaaggt attgaggtga tgcgtgcact acgcaaaggc caggcctcag
    10381 cattttatta tggtgatccc ctgggcgaaa tgcgcctggt aagcagagtt tttgaaatgt
    10441 aaggcctttg aataagacaa aaggctgcct catcgctaac tttgcaacag tgcc
//
