LOCUS       NZ_CP048370             2089 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain 164 plasmid pC-F-164_C, complete sequence.
ACCESSION   NZ_CP048370
VERSION     NZ_CP048370.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951916
            Assembly: GCF_010365525.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: C-F-164.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010365525.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:40:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,917
            CDSs (total)                      :: 4,797
            Genes (coding)                    :: 4,534
            CDSs (with protein)               :: 4,534
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 96 of 263
            Pseudo Genes (incomplete)         :: 167 of 263
            Pseudo Genes (internal stop)      :: 55 of 263
            Pseudo Genes (multiple problems)  :: 49 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048370.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="164"
                     /isolation_source="environment"
                     /db_xref="taxon:562"
                     /plasmid="pC-F-164_C"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
                     /note="This genome was originally submitted with the wrong
                     strain name and BioSample. It was corrected on March 18,
                     2020."
     gene            complement(join(2085..2089,1..262))
                     /locus_tag="GZS07_RS24255"
                     /old_locus_tag="GZS08_24245"
                     /old_locus_tag="GZS08_RS24245"
     CDS             complement(join(2085..2089,1..262))
                     /locus_tag="GZS07_RS24255"
                     /old_locus_tag="GZS08_24245"
                     /old_locus_tag="GZS08_RS24245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(266..1231)
                     /locus_tag="GZS07_RS24260"
                     /old_locus_tag="GZS08_24250"
                     /old_locus_tag="GZS08_RS24250"
     CDS             complement(266..1231)
                     /locus_tag="GZS07_RS24260"
                     /old_locus_tag="GZS08_24250"
                     /old_locus_tag="GZS08_RS24250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(607..838)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 aatgcgttct taaaaatcca aacttgatac cgtttaaatc tattttcaca aaaagagttt
       61 ttaccctctc gtttgttttc tgaatatata aaattaattc atcctcaaaa ccttcgtgcg
      121 gatgaaaacc aaatgtgcca aaacttaatc catcaataag caaaataagg tttttaaacc
      181 tgtcaggaat caccgcttca cttcttaaaa aaaataaacc aatagtctta tttccattca
      241 ggtattcaat attaatattc atacgttaat ccgatttatc cttctcaggg gcgcgtttgt
      301 aacgccgttt acctgaatcc cagacaaacg ccgtccgctt cccgtcatca gtgccatccc
      361 ctacatcgtc ggcaatgaca agatcctcat tggtttctcg atccaactgg aggacgtttt
      421 ttagcgcccc accggtcgag ataaaacggc gcttgtgaag ctgccgcgtc agctcaagaa
      481 accactcagg atcgtttgcc atatcttccg gtttaacgga gtatttcagc gtttcagcaa
      541 ccgcgctttg cagttgctcg gcaacgttgg ccacaacctc acctgtctta gtttttactg
      601 cccgaatatc gatattcggc tcatagttca cccgcaagca atcgcgccag agttctaccc
      661 aacgttcgtg cttaacgtag ttcttccctt taaaccaaga aggttgcacc atcagcagac
      721 agtgaaaatg cggatgtgcg ctgccatcct tacctcgcgt cacctccgta gccctgatcc
      781 acccctgaac aggtgatagc tcctttcgct tttccattcg cttaaacgcc gcattcattg
      841 ctgtaaggac tgttccaagt tcacctatct cgcagttcct gacagttaac gtcagaaaca
      901 accatcggga agacgggtaa tccacaacga ttttcggtag agcctgataa aaacgggctt
      961 gccacatgag ggttcttctc cactggcaga cagggcaatg acggacacgg caaaattgcg
     1021 cactacgaag ccgtaggcgc gtttctccgg tttccatgat ggtagaccaa ccaaaacgta
     1081 acaaaccgct acaggaggcc atacggagtg catagcgctc gaattcagca gcagtctggt
     1141 agataccccc gacagaatcg gtaagcgtgc gctgggtatc ccaaactgca tcacgggggc
     1201 tgtagtccga aaggaattta tcttctgaca ttaagcctgc tccgatctgg gcaggctgtg
     1261 atttgatgtg gtatattcat cacgtacctg tgcattgcga gccagtacat gtcaccagag
     1321 tttgccgctg aggtgacaca ttcgaaatgt tggggagtgt tgacgcactc cccaacgccg
     1381 attaaaaacg cctcaattct gctccatttt ctttgacctg tcaaaaccca ctcaaagcct
     1441 cgtcattact gggctaaacc actatttcat atccgccatt tatcccttaa gtataaatac
     1501 aaggatcacg cgcgagaatt gcgcaaaaaa taaccacaat tttaaccccc caaaacaagc
     1561 acccccgaac ggcttacccc aaccttttcc acacacccga tcaccaaaac agggaataaa
     1621 aacaaaaccc tgtcttacag gtggatggcc tcacttcgtg aaaccatcca ccccgcataa
     1681 gttttttaat cggtggtgtc gtcaatcact aaatcggaac cccaaagggg acccccacga
     1741 tttagcattt gacgagatta actctcggag atggcatcat gcttttaacg gactttaaaa
     1801 aacggtgaac ggatgcggat tttaatccgt gtctgtgagg cgttgagcaa cgagcaaagc
     1861 gaggcggtag aaagagagcc agcggcaggc tcaggacgat aatccgggga aaatgtacca
     1921 ctcgcagagt gttaacattt gacatcggat tgtctatcct gcattggtgt cgagttggca
     1981 aggctgagga taatgctgag gataatgcag gatagacaga gcactagaga ttgcatgcaa
     2041 tctcgtacct atcacctcag cctgccgctg gctcttttcc agaactaag
//
