LOCUS       NZ_CP048366             2101 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain 53 plasmid p53_G, complete sequence.
ACCESSION   NZ_CP048366
VERSION     NZ_CP048366.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951918
            Assembly: GCF_010365485.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: 53.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010365485.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:49:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,176
            CDSs (total)                      :: 5,058
            Genes (coding)                    :: 4,850
            CDSs (with protein)               :: 4,850
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 208
            CDSs (without protein)            :: 208
            Pseudo Genes (ambiguous residues) :: 0 of 208
            Pseudo Genes (frameshifted)       :: 75 of 208
            Pseudo Genes (incomplete)         :: 139 of 208
            Pseudo Genes (internal stop)      :: 42 of 208
            Pseudo Genes (multiple problems)  :: 43 of 208
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048366.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="53"
                     /isolation_source="environment"
                     /db_xref="taxon:562"
                     /plasmid="p53_G"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            join(1989..2101,1..154)
                     /locus_tag="GZS09_RS25540"
                     /old_locus_tag="GZS09_25540"
     CDS             join(1989..2101,1..154)
                     /locus_tag="GZS09_RS25540"
                     /old_locus_tag="GZS09_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            1020..1985
                     /locus_tag="GZS09_RS25545"
                     /old_locus_tag="GZS09_25545"
     CDS             1020..1985
                     /locus_tag="GZS09_RS25545"
                     /old_locus_tag="GZS09_25545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          1414..1645
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 attaagtttt ggcacatttg gttttcatcc gcacgaaggt tttgaggatg aattaatttt
       61 atatattcag aaaacaaacg agagggtaaa aactcttttt gtgaaaatag atttaaacgg
      121 tatcaagttt ggatttttaa gaacgcattc ttagttctgg aaaagagcca gcggcaggct
      181 gaggtgatag gtacgagatt gcatgcaatc tctagtgctc tgtctatcct gcattatcct
      241 cagcattatc ctcagcatta tcctcagcct tgccaactcg acaccaatgc aggatagaca
      301 atccgatgtc aaatgttaac actctgcgag tggtacattt tccccggatt atcgtcctga
      361 gcctgccgct ggctctcttt ctaccgcctc gctttgctcg ttgctcaacg cctcacagac
      421 acggattaaa atccgcatcc gttcaccgtt ttttaaagtc cgttaaaagc atgatgccat
      481 ctccgagagt taatctcgtc aaatgctaaa tcgtgggggt cccctttggg gttccgattt
      541 agtgattgac gacaccaccg attaaaaaac ttatgcgggg tggatggttt cacgaagtga
      601 ggccatccac ctgtaagaca gggttttgtt tttattccct gttttggtga tcgggtgtgt
      661 ggaaaaggtt ggggtaagcc gttcgggggt gcttgttttg ggggtttaaa attgtggtta
      721 ttttttgcgc aattctcgcg cgtgatcctt gtatttatac ttaagggata aatggcggat
      781 atgaaatagt ggtttagccc agtaatgacg aggctttgag tgggttttga caggtcaaag
      841 aaaatggagc agaattgagg cgtttttaat cggcgttggg gagtgcgtca acactcccca
      901 acatttcgaa tgtgtcacct cagcggcaaa ctctggtgac atgtactggc tcgcaatgca
      961 caggtacgtg atgaatatac cacatcaaat cacagcctgc ccagatcgga gcaggcttaa
     1021 tgtcagaaga taaattcctt tcggactaca gcccccgtga tgcagtttgg gatacccagc
     1081 gcacgcttac cgattctgtc gggggtatct accagactgc tgctgaattc gagcgctatg
     1141 cactccgtat ggcctcctgt agcggtttgt tacgttttgg ttggtctacc atcatggaaa
     1201 ccggagaaac gcgcctacgg cttcgtagtg cgcaattttg ccgtgtccgt cattgccctg
     1261 tctgccagtg gagaagaacc ctcatgtggc aagcccgttt ttatcaggct ctaccgaaaa
     1321 tcgttgtgga ttacccgtct tcccgatggt tgtttctgac gttaactgtc aggaactgcg
     1381 agataggtga acttggaaca gtccttacag caatgaatgc ggcgtttaag cgaatggaaa
     1441 agcgaaagga gctatcacct gttcaggggt ggatcagggc tacggaggtg acgcgaggta
     1501 aggatggcag cgcacatccg cattttcact gtctgctgat ggtgcaacct tcttggttta
     1561 aagggaagaa ctacgttaag cacgaacgtt gggtagaact ctggcgcgat tgcttgcggg
     1621 tgaactatga gccgaatatc gatattcggg cagtaaaaac taagacaggt gaggttgtgg
     1681 ccaacgttgc cgagcaactg caaagcgcgg ttgctgaaac gctgaaatac tccgttaaac
     1741 cggaagatat ggcaaacgat cctgagtggt ttcttgagct gacgcggcag cttcacaagc
     1801 gccgttttat ctcgaccggt ggggcgctaa aaaacgtcct ccagttggat cgagaaacca
     1861 atgaggatct tgtcattgcc gacgatgtag gggatggcac tgatgacggg aagcggacgg
     1921 cgtttgtctg ggattcaggt aaacggcgtt acaaacgcgc ccctgagaag gataaatcgg
     1981 attaacgtat gaatattaat attgaatacc tgaatggaaa taagactatt ggtttatttt
     2041 ttttaagaag tgaagcggtg attcctgaca ggtttaaaaa ccttattttg cttattgatg
     2101 g
//
