LOCUS       NZ_CP048358             3223 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Raoultella ornithinolytica strain 23 plasmid p23_I, complete
            sequence.
ACCESSION   NZ_CP048358
VERSION     NZ_CP048358.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951919
            Assembly: GCF_010365445.1
KEYWORDS    RefSeq.
SOURCE      Raoultella ornithinolytica
  ORGANISM  Raoultella ornithinolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Raoultella.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: 23.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:32:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,190
            CDSs (total)                      :: 6,065
            Genes (coding)                    :: 5,954
            CDSs (with protein)               :: 5,954
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 38 of 111
            Pseudo Genes (incomplete)         :: 90 of 111
            Pseudo Genes (internal stop)      :: 21 of 111
            Pseudo Genes (multiple problems)  :: 31 of 111
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048358.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Raoultella ornithinolytica"
                     /mol_type="genomic DNA"
                     /strain="23"
                     /isolation_source="environment"
                     /db_xref="taxon:54291"
                     /plasmid="p23_I"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            complement(join(2326..3223,1..599))
                     /locus_tag="GZS10_RS31485"
     CDS             complement(join(2326..3223,1..599))
                     /locus_tag="GZS10_RS31485"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_247652451.1"
                     /translation="MMIVKFHPRGRGGGGGPVDYLLGKDRQRDGASVLQGKPDEVRELI
                     DASPYAKKYTSGVLSFAEQDLPPGQREKLMASFERVLMPGLDKDQYSVLWVEHRDKGRL
                     ELNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRVLVSPSA
                     LPEAKQEAAQAITSGLLALASSGELKTRQDVTEALESAGFEVVRTTKSSISIADPDGGR
                     NIRLKGAIYEQSFNAGEGLRAEIESAAADYRRDAESRIQRAREVCQSGTERKREENQRR
                     HPRPRPEAVLSHEPAHERDAAHGQPDVADHRSGLRAADSVERGHSVVAGAADTRELYDH
                     PGAEEHAGHAVREEQRRTALDLRRAETPLREGEPGSGTVRRGLELDDTGGEIAHDGAGK
                     TVAERIRAATAGLLEKAGRVGERLRGMADDVWSYSTGERGAERARHGLEQAGAEFERAA
                     APVVERLNAIETHRQQERAVQHQKALELERSQWQHHGPSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(586..909)
                     /locus_tag="GZS10_RS30860"
                     /old_locus_tag="GZS10_30860"
     CDS             complement(586..909)
                     /locus_tag="GZS10_RS30860"
                     /old_locus_tag="GZS10_30860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1548..1718
                     /locus_tag="GZS10_RS30865"
                     /old_locus_tag="GZS10_30865"
     CDS             1548..1718
                     /locus_tag="GZS10_RS30865"
                     /old_locus_tag="GZS10_30865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004193911.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNWVHGAEIV
                     ENAYRVLEVKA"
                     /name="hypothetical protein"
     gene            complement(1927..2259)
                     /locus_tag="GZS10_RS30870"
                     /old_locus_tag="GZS10_30870"
     CDS             complement(1927..2259)
                     /locus_tag="GZS10_RS30870"
                     /old_locus_tag="GZS10_30870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_016807542.1"
                     /translation="MNTYIFLGGAIVAEVIGTIFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     ARG             complement(1928..2259)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          complement(1006..1197)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          1177..1660
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.756"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          complement(4..623)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.754"
                     /coverage="95.0"
                     /evalue="1.33e-121"
     Typing          complement(2330..3222)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.667"
                     /coverage="99.0"
                     /evalue="4.69e-174"
ORIGIN
        1 ctttccagcg cctcagtgac gtcctgacgc gttttcagct ccccggatga ggcaagggca
       61 agcaggccgc tcgtaatcgc ctgggcggct tcctgcttcg cttccggcag cgcggaaggc
      121 gagaccagca cccgccggtt ctccggcgcg ttcgggtcat gcagccccag cctgccgttc
      181 actatggtct gccaggcatc gatgcgcgga cggtcggcgc ggtcgtaata cggctgtagc
      241 cgcttgccgg tcagaagctc cgtgttcggg atcaggaagt tcagctccag ccgccccttg
      301 tcccggtgct caacccacag cacgctgtac tggtctttat cgagtccggg catcagaacc
      361 cgctcgaaac tcgccatcag cttttcgcgc tggccgggcg gtaaatcctg ttcggcaaaa
      421 gacagaacgc cggaagtgta ctttttggcg tagggcgagg cgtcgataag ctcccggact
      481 tcatccggct tcccctgcag aacgctggcg ccgtcgcgct ggcgatcttt acccagcaga
      541 taatcgaccg gcccgccgcc gccgccgcgc ccccgaggat gaaacttaac gatcatcatc
      601 agcacccttt tccagtacgg catgccgcaa ccgctcgagt ccggcatcga tggccatcag
      661 cgcggcgaca atctgcacgc ggtcgtgtcc gcttccacca ccggcgttaa cctggcgggc
      721 aatctggtta aggttgttcc ccatgccggc aagctgacga agcagcgccg gcgagatcga
      781 cggaagtttg ccggcgcgtg cgggcttctc gtccaggcac gtctgccgca tccaggccgc
      841 gagttgctta ccctcgcagc gctccagcag acgccggtgc tcgtcctcag tcacccacat
      901 ggtgagcatt ttgctgcgtt tgtctgccaa tccggcctcc tgataacgcc gggacggacg
      961 ggaaatcttt tcccggtcgg cacggtgttg gacaagccgc aggcgcggca gtggttttag
     1021 cgggtttcgg ggcgcagccc tgaaccagtc acgtagcgct agcggagtgt atactggcta
     1081 aacatgttat gacggagtgg caatttatga aagtgcttca tgtgacaggg gaaaaatgcg
     1141 gctgccgggc gtaagtgcaa aatgcaggtg gtgatgaaat gacgcctcct cgctcactcg
     1201 gtcgctacgc tcctgccgtg agactgcggc gggcgttacc agctcacaga ttacgggata
     1261 agcaaatgcc gctcgaagca ggaaaggcca agaaaaggat gcggagttgc cgtttttcca
     1321 taggctccgc ccccctgacg agtatcacga aaatgacgct caaatcagcg gtggcgaaac
     1381 ccgatgcggc ttaaagatac caggcgtttc cccctggtgg ctccctcgtg ctcccccgca
     1441 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcggttt ctcattccac
     1501 gcctgaaact cggttccggg taggcagttc gctccaggat ggactgtatg cacgaacccc
     1561 ccgttcagtc cgactaccac gcccgttccg gtaactatca acttgagtcc aacccggaaa
     1621 gacacgacaa atcgccagtg gcggtagcca ttggtaactg ggttcacgga gcagagattg
     1681 tagagaatgc ctacagagtt cttgaagtga aagcctgagt gcggctacac tgaaaggaca
     1741 gtttaggtat ctgcgctccg tgaaagccag ttaccatcgg aacaaagagt tggtagctct
     1801 gaattccggc aaacgaacca ccgttggtag cggtggtttt tttgtttaca gggaaaaaga
     1861 ttacgcgcag aaagaaagga tctcaaggaa atccttttgc tttggccaat atgcttcggt
     1921 aaaaagttaa tgcgcagaag attttgaaaa aatattaata accaaaactc caacgcaaat
     1981 aaggaacatt cctgcaattg cgggccaatc aagtttttga ccgctaacca accaaccagc
     2041 taggcttatt agtacaattc ccgcaccaga ccaaattgcg taagcaatac cggtaggtat
     2101 attttccaat gtttgggaaa gcagataaaa ggcagcgcaa taacttattg cagttcctac
     2161 agacggccac aggcgtgtaa atccttctga atatttcata aagatggtgc ctatgacttc
     2221 agccacaata gcccctccca ggaaaatata tgtattcata aaaatacctc atgttgaaaa
     2281 atttaaatat ctttacataa caactaatta atagaacccg gcttatcata gcgaaggacc
     2341 atgatgctgc cactgcgatc gttccagctc cagcgctttc tggtgctgca ccgcccgctc
     2401 ctgctgccgg tgagtttcta tcgcgttcag cctttcgaca accggcgcag ctgctcgctc
     2461 aaactctgca cctgcctgct caagaccgtg acgcgctcgt tcagcgccgc gttctcccgt
     2521 tgaataagac cagacatcgt ccgccattcc gcgaaggcgt tctcccactc gtccagcctt
     2581 ttcgagtagt cctgctgtag ctgctctaat gcgctcagca actgtttttc cagctccgtc
     2641 atgtgctatt tccccgccag tatcatccag ctcgagtcct ctccgaactg tcccgcttcc
     2701 gggttcaccc tcacgcagcg gcgtctctgc tcgccgcagg tcgagagctg tacgccgctg
     2761 ttcctctctg acagcatggc ctgcgtgctc ttctgctccc ggatggtcgt atagttctcg
     2821 agtatctgct gcccctgcca ccacagaatg cccgcgctcg acgctatcag cagcgcggag
     2881 accagaacga tggtcagcca cgtctggctg accatgcgca gcatcccttt cgtgcgctgg
     2941 ctcatggctg aggacagctt ccggtcgtgg tcgaggatgg cggcgctgat tctcttctcg
     3001 ctttcgctca gttccgctct gacagacttc tcgtgctcgc tgaatgcggc tttcagcatc
     3061 tcgccggtag tctgctgctg cgctttcgat ttctgctcta agtccttcgc cagcgttaaa
     3121 agactgttca tagatggctc ccttcagtcg gatgtttcgc cccccgtccg ggtcggcaat
     3181 gctgatgctg ctttttgtgg tgcgcaccac ctcaaaacct gcg
//
