LOCUS       NZ_CP048357             3325 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Raoultella ornithinolytica strain 23 plasmid p23_H, complete
            sequence.
ACCESSION   NZ_CP048357
VERSION     NZ_CP048357.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951919
            Assembly: GCF_010365445.1
KEYWORDS    RefSeq.
SOURCE      Raoultella ornithinolytica
  ORGANISM  Raoultella ornithinolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Raoultella.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: 23.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:32:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,190
            CDSs (total)                      :: 6,065
            Genes (coding)                    :: 5,954
            CDSs (with protein)               :: 5,954
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 38 of 111
            Pseudo Genes (incomplete)         :: 90 of 111
            Pseudo Genes (internal stop)      :: 21 of 111
            Pseudo Genes (multiple problems)  :: 31 of 111
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048357.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Raoultella ornithinolytica"
                     /mol_type="genomic DNA"
                     /strain="23"
                     /isolation_source="environment"
                     /db_xref="taxon:54291"
                     /plasmid="p23_H"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            221..412
                     /locus_tag="GZS10_RS30830"
                     /old_locus_tag="GZS10_30830"
     CDS             221..412
                     /locus_tag="GZS10_RS30830"
                     /old_locus_tag="GZS10_30830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032421723.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            412..834
                     /locus_tag="GZS10_RS30835"
                     /old_locus_tag="GZS10_30835"
     CDS             412..834
                     /locus_tag="GZS10_RS30835"
                     /old_locus_tag="GZS10_30835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_162661897.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKGIKVID
                     TSELIRVFGSLDGVIDAVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHDPSSPAHFPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1180..1422)
                     /locus_tag="GZS10_RS30840"
                     /old_locus_tag="GZS10_30840"
     CDS             complement(1180..1422)
                     /locus_tag="GZS10_RS30840"
                     /old_locus_tag="GZS10_30840"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            1808..2128
                     /locus_tag="GZS10_RS30845"
                     /old_locus_tag="GZS10_30845"
     CDS             1808..2128
                     /locus_tag="GZS10_RS30845"
                     /old_locus_tag="GZS10_30845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017134632.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_048333940.1"
                     /translation="MLNKFSSPFELFAKDAVELNMLSLKSKLLIIIAEMIRGEGWSQAA
                     AADKLGVSQPRVSNLMNGHVSKFAIDTLLEMLCRIGFVVDVTFRPHDEESPLHVSVKKA
                     VV"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(2154..2474)
                     /locus_tag="GZS10_RS30850"
                     /old_locus_tag="GZS10_30850"
     CDS             complement(2154..2474)
                     /locus_tag="GZS10_RS30850"
                     /old_locus_tag="GZS10_30850"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_162661898.1"
                     /translation="MAQLELKVEKGVEKEFRALENRKVQEAFAVDLEMIMNGLKPLSSS
                     KPLNGLGKGVFELKKNGRPAYRCVYAVKNNTLYVLHVYSKTSDGTPKHHEDTIKKRFKS
                     VK"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          complement(2832..3312)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.09"
                     /coverage="91.0"
                     /evalue="7.96e-151"
ORIGIN
        1 cttttttgtg atgcttgtca ggggggcgga gcctatggaa aaacggccgc tgggcggcct
       61 cttcttttcc gcttcccttg atcgctcggt tttctcgagc ttttataaga acggtcttgc
      121 cgctcgccgc agccgaacga ccggagcgta gcgactgagt gagcgaggaa gcggaaaaga
      181 gactgttctt acactgagca ctgacgctct gaggcttctt atgactaaac aacagaaaac
      241 agcgctgaac atggctaaat tcatccagaa tcagtctctg ctcctgctgg agaagcttaa
      301 cgagcttgat ctcgatgctg aggccgacct ttgtgagaaa ctccacgatg atgcggagca
      361 tcttttccgt acactgtcct cgagactgga tgaattacag gatggtaact gatggccaga
      421 gtgtctatta gtgaagcagc acggcttgtt aaggttagtc gccctactat ttacaagatg
      481 attaacagtg gtaagttaag ttacacctct gttgtaaagc acgggaaggg tattaaagtt
      541 attgatacat cagagcttat tagagtgttt ggttctcttg atggtgtaat cgatgctgta
      601 aaatatgatg taaaaagtga cgcagaatct acaggtatta acagcgttgg tttacatgat
      661 ttacaacata gaatcgcttt acttgaagct gaaaacgatg gtcttaaagg ggctgttaaa
      721 gcacgcgatg agcatattga ttcccttcgc caggctatgc agcttttgga gcacaagcac
      781 gacccctctt caccggctca ttttccgtgg tggaaattct ggaaaaaatc ctgatcctgc
      841 catgtttcag ttttgctctc cggcgatccc ggggagttcc tgaaccttcc caaaaatcgc
      901 cagatctctg aacgctcctc accactaaaa atacccacac actacgcaaa attaaaattt
      961 tgcagataga cctttagttt caattagtta tctattgtaa attacgcaat tttatttttt
     1021 gtaaggtgaa tccgagtcgt gggcgactct atggcgaatc gctgacgaat tgcccacgat
     1081 tcatatgtga atcacgcttt attatcatat tgttatgatg aatcgactct tgattcaatt
     1141 catcactttg ggtgtttttt gctcatggtg actcttgatt caattcacga ttcgaatcgc
     1201 cggtgaatcg tgtggtgagt cgtccggcga cttgccataa cgtgattgcc ggccgtcgac
     1261 gaacccgatt cctgaaggtg ctgaaaaccg gttttccggc tgcgccggca acgcttcacg
     1321 gccacgcaat aaattgcgtc cccgttcacc gttttcggag gagaagaacg ccggaaacag
     1381 gacttcccct ggctgaatgg tgtgaatttc cgcgctatgc acttgcgcgc atactcatgc
     1441 atgccgtaaa aacagagcct gcgcgtttct ggcgggtttt cgggtggttt gttgcctgtt
     1501 ttaccggttt cccgtcagaa acgccctgag ggcctctcag gcggtgctga taacggatgt
     1561 tatggtaaat ccgttctaaa gcacgatagt ttttgccaac atacactaca aaattgttga
     1621 cgaaaggggt tgacgggatg aagtgaagcg aactataaca agtcttcagt gtctatatat
     1681 atacttatat atcttgcact ggggtgtgta ttgcgtataa tacagcgccc atgagatgtg
     1741 gattgagtgc tttttgacca ctccattcta atgaacatgc aacgcataga cgaagaggag
     1801 gatttgtatg ctaaataaat ttagcagtcc gtttgaactc tttgctaaag acgcagttga
     1861 gttgaacatg ctcagcctga aatcaaagct gcttataatc atcgctgaaa tgatcagagg
     1921 cgagggttgg tcgcaagcgg ctgctgcgga taagcttgga gttagtcagc cccgcgttag
     1981 taacttaatg aatgggcatg tatccaagtt tgccattgat acgctccttg agatgctgtg
     2041 ccgcattgga tttgttgtgg atgtgacctt tcggccgcat gatgaagaaa gtcctttgca
     2101 tgtgagcgta aaaaaggcgg ttgtgtgaga accgccttgc gtaatttgat tgtttatttt
     2161 actgatttaa aacgtttttt tattgtgtct tcgtgatgct ttggtgtgcc gtccgatgtc
     2221 ttactgtata cgtgaaggac gtatagtgtg ttgtttttta ctgcataaac acatctgtaa
     2281 gccgggcggc cgtttttctt tagttcaaaa actccttttc ctaatccatt tagtggcttt
     2341 gatgatgaca acggtttgag cccgttcatt atcatttcga ggtctacagc aaaagcctct
     2401 tgcaccttcc tgttttctag tgcgcgaaat tccttctcta cacctttctc cactttcaat
     2461 tctagttgtg ccataaatcc tcctgcactc gtttagatta aggtgttcat acataaaccc
     2521 tcgattgttt agtgtttgcc agagccatga atttcagggt gctctgaccc aatttaacct
     2581 aggaagtata acctgggcat attctgaaat taaagaggtg acatataaat atttgtataa
     2641 attagattgt tttttcgccg aaggtcattt ttgccatctc ttatgccgga acatggctat
     2701 taaaatagtg gatgaaatct gtttttcttt taaaaaaaga agttatatct aaaggtggtt
     2761 ttgcgttgag gtcgtcaaag agggggaaga tccttgacga tcgtttgggt ggcggcgtag
     2821 tctagcgaca gaaaagaaaa aaccgcctgg ggaggcggtt ttttcaacgg tttagtaggt
     2881 tggggaactt actaaaccaa ggtaactggc tattcacgga gcgcagatac caaatcctgt
     2941 cctttcagtg tagccgtagt caggccgcca cttcaagaac tcttgcacac taaagtgcgt
     3001 aatcaagctc cgtgaaccct gttaccaatg gctgctgcca gtggcgcctc gtcgtgtttt
     3061 tccgggttgg aatcaagttg aaagttaccg gaaaggcgta gcagtcggac tgaacggggg
     3121 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
     3181 gaatgagata aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
     3241 ggagagcgca cgagggagcc accaggggga aacgcctggt atctttatag tcctgtcggg
     3301 tttcgccacc actgatttga gcgtc
//
