LOCUS       NZ_CP048356             3825 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Raoultella ornithinolytica strain 23 plasmid p23_G, complete
            sequence.
ACCESSION   NZ_CP048356
VERSION     NZ_CP048356.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951919
            Assembly: GCF_010365445.1
KEYWORDS    RefSeq.
SOURCE      Raoultella ornithinolytica
  ORGANISM  Raoultella ornithinolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Raoultella.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: 23.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:32:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,190
            CDSs (total)                      :: 6,065
            Genes (coding)                    :: 5,954
            CDSs (with protein)               :: 5,954
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 38 of 111
            Pseudo Genes (incomplete)         :: 90 of 111
            Pseudo Genes (internal stop)      :: 21 of 111
            Pseudo Genes (multiple problems)  :: 31 of 111
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048356.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Raoultella ornithinolytica"
                     /mol_type="genomic DNA"
                     /strain="23"
                     /isolation_source="environment"
                     /db_xref="taxon:54291"
                     /plasmid="p23_G"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            join(3461..3825,1..37)
                     /locus_tag="GZS10_RS30805"
                     /old_locus_tag="GZS10_30805"
     CDS             join(3461..3825,1..37)
                     /locus_tag="GZS10_RS30805"
                     /old_locus_tag="GZS10_30805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            842..1114
                     /locus_tag="GZS10_RS30810"
                     /old_locus_tag="GZS10_30810"
     CDS             842..1114
                     /locus_tag="GZS10_RS30810"
                     /old_locus_tag="GZS10_30810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004897311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_019405140.1"
                     /translation="MATDAQVKEINALIKKYPDSCSICHEVYDEDDVTYTVFGYDRKGK
                     IQVTTGCCAGMLTEPVLLGVCGCFDPEERDEIMQNHPMAKQFFTE"
                     /name="hypothetical protein"
     gene            complement(1922..2083)
                     /locus_tag="GZS10_RS30995"
     CDS             complement(1922..2083)
                     /locus_tag="GZS10_RS30995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071784511.1"
                     /translation="MQEPPVQPDRCAFSGNYRLESNPERHDLTPLAAATGKQDVREIYE
                     EFLKWWPD"
                     /name="replication initiation protein"
     gene            3270..3461
                     /locus_tag="GZS10_RS30820"
                     /old_locus_tag="GZS10_30820"
     CDS             3270..3461
                     /locus_tag="GZS10_RS30820"
                     /old_locus_tag="GZS10_30820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2434..2648
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="83.562"
                     /coverage="100.0"
                     /evalue="1.62e-49"
     Typing          complement(1937..2453)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.847"
                     /coverage="87.0"
                     /evalue="5.5e-153"
ORIGIN
        1 cccggcgcca tggtggaaat tctggaaaaa atcctgatcc cgccatgctt ccattttgct
       61 ctccggccat tccggggagt tcctgaacct tcccaaaaat cgtcagatcc ctgaacgctc
      121 cttaccacta aaaataccca cacactacgc aaaattaaaa ttttgcagat agacctttat
      181 tttcaatcag ttatctgttt taaattacgc aattttattt tttgtaaggt gaatccgagt
      241 cgtggacgac tctatggcga atcgccgatg acttgcccac gattcatatg tgaatcacgc
      301 catataatca gtacgttatg atgacttgac tctagagtcg acttgccggg ttggtggctt
      361 tttgaccatg gcgactctag agtcaagtca cgagtcgaat cgccggtgaa ttgtctggtg
      421 agtcgtccgg cgacttgcca taacgtgatt gccggccgtc gacgaacccg attcctgatc
      481 gtgctgaaaa ccggttttcc ggctgcgccg gcaacgcttc acggccacgc aataaattgc
      541 gtccccgttc accgttttcg gataggaaga acgccggaaa caggacttac cctggctgaa
      601 tggcgtgaat ttccgcgcta tgcacttgcg tgcatactca tgcatgccgt aaaaacagag
      661 ccagcgcgtt tctggcggat tttcgggtgg tttgttgcct gttttaccgg tttcccgtca
      721 ggaacgccct gaggccgttt tcgcggtgcg cgtaatggga cgttatggta aatcaacggc
      781 ggatgcctga gtctgtgtta gagtcagcgg attgtgataa tcagtgactc agggagatgt
      841 tttggctact gacgctcagg taaaagaaat taatgcactc atcaaaaaat acccggactc
      901 atgctccatc tgtcatgaag tctatgatga ggatgatgtg acctacaccg tttttgggta
      961 tgaccggaaa ggtaaaatac aggtcaccac gggatgctgt gccggaatgc tgacagagcc
     1021 ggttctgctg ggtgtctgtg ggtgctttga cccggaagaa cgtgacgaga tcatgcagaa
     1081 tcaccccatg gcaaagcagt ttttcacaga gtgattccgt atctgttgag tctgaagccc
     1141 gccttgtgcg ggctttttca tatctgcgtg aaatgagacg ttatggtaaa tctctaaagg
     1201 caatatttcc cgtcccttgg agcgaactac tctgtatttg atctttggtg ttgatacccc
     1261 gctgcttgca gagggggtat tcattatcgt tgttgtttgc tgtaattgtc taaaatatat
     1321 ttggggatag atacgggtac acattcagca gatattatgt ttttcccgga tatattatga
     1381 taaatgtctt tgtttgcaat gattttatct ttgaattcaa tgcattcttc attcagaatg
     1441 aaggtgttaa caacattgtc tttcattccc aaatttgcta attctgaaga acaacttgca
     1501 atattattcg tgttaaaaca agttgttagt attattgcgt atatgtatgg cattaaataa
     1561 acctatttaa aaaagtacgg aatgatagtg atcttgttcg gaaatatact agtcagactt
     1621 taagtctcag ggatatgaac ctagaacatc gttgtattaa cttgtttgag tgttccaatc
     1681 ctgaatattg atgttatttg agtataaagg aaacataata aatatcacaa ggatattggt
     1741 taactattta tttttaaata atttcttgag atctgacgtt ggacgtcgta gtctcttgcc
     1801 atgtaaacga aaaaaccacc ttgcagggtg gtttttcgaa ggttcagtaa accggcaagt
     1861 ttctgaaccg tggtaacagt cttgtgcgag acgtcaccaa atctgtcctt ttagtgtagc
     1921 ctcagtctgg ccaccacttc aagaactctt cgtaaatctc tcgcacatcc tgtttaccag
     1981 tggccgctgc cagtggcgtt aagtcgtgtc tttccgggtt ggactcaaga cgatagttac
     2041 cggaaaaggc gcagcggtcg ggctgaacgg ggggttcttg catacagccc agcttggagc
     2101 gaactgtcta aacggaacgg ggcgtggtga tttaggtaaa gcttccacca ctacacggat
     2161 gccgcaggac gggaacagga gagcgcaaga gggagccacc agggggaaac gcctggtatc
     2221 tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt
     2281 caggggggcg gagcctatgg aaaaacggct ttgccgcggc cttatcgtat ccctgttaag
     2341 tttcttcctg gcatccacca ggaaataaat ttccgcccca ttagtaagcc tgcaccgctc
     2401 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa tatcacctga
     2461 accagcatct tctcctgacg ccggaaagcc tcatttcact cctgccacat gaagcacttt
     2521 ggcaaatcac cacttcggtt taaaatattt agccagtatg cactccgcta gcgctacgtg
     2581 actggttcag ggctgcgccc cgaaacccgc taacacccct cagctactgg atatcacacc
     2641 atgccatctg agtatgatga acaaccggat gcgactgatg ctctgtttcc cggcgaatat
     2701 gacctttttg acttaatggc tcatgttact gaagagaacc tgcacgcccc ggtggattct
     2761 ggcccgcctg tcggccggga agcgttatag ctttttcgct ggccagcgta catgtaatac
     2821 gctggtatta catgttattt gtttattttc atgttgttac agatgattgt aatacgttgc
     2881 gtattacgct aatacacgaa ataactccat ttttttcatt taattcatgc agttgcgata
     2941 aaaatcgcgg ctgcgttagg tgtatgacca tttaaggggc aaaaaattat cattctggct
     3001 gggtgatctg cgcaggttaa ggatcatcgg cgacgaaaaa gcgtctttaa ctgcttcgtt
     3061 catgcttgca tgaacggatt atttttccag actacaggta aactacagga agactacaaa
     3121 agttgatgat ttaatcctgc gttttagtgc gatgactaca gataaactac aaaataacta
     3181 caggataacc tcagatccgt tctctgaacc attcgtacat ccgaccccct taccggttta
     3241 cactgagcac tgatgctctg agggccctta tgacgaaaca acagaaaaca gcgctgaaca
     3301 tggctaaatt catccagaat cagtctctgc tcctgctgga gaagcttaac gagcttgatc
     3361 tcgatgttga ggcggacctt tgtgagaaac tccacgatga tgcggagcat cttttccgta
     3421 cactgtcctc gagactggat gcattacagg atggtaactg atggccagag tgtctattag
     3481 tgaggctgcc cgactgacgg gtaagagtag aacaacactg cacaggttaa ttaaagcagg
     3541 tgacctgtcc acctgtagcg gtgaacgcaa tgcaaaaatg gtagatactt cagagctttt
     3601 acgtgtcttt ggtccctttg aacaaccgaa gggtgaacac cattctggac aggtctctgg
     3661 acagcgtgat acagcctctg ctgaccagag tgaacaggtg attcgtcatc tgaaacagga
     3721 gattgaacac ctgaacacct tacttttgtc caaggattcg cacattgaca gcctgaaaca
     3781 ggccatgctc cttcttgaaa atcaccaggc aaaattaact gaatc
//
