LOCUS       NZ_CP048355             4340 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Raoultella ornithinolytica strain 23 plasmid p23_F, complete
            sequence.
ACCESSION   NZ_CP048355
VERSION     NZ_CP048355.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951919
            Assembly: GCF_010365445.1
KEYWORDS    RefSeq.
SOURCE      Raoultella ornithinolytica
  ORGANISM  Raoultella ornithinolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Raoultella.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: 23.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:32:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,190
            CDSs (total)                      :: 6,065
            Genes (coding)                    :: 5,954
            CDSs (with protein)               :: 5,954
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 38 of 111
            Pseudo Genes (incomplete)         :: 90 of 111
            Pseudo Genes (internal stop)      :: 21 of 111
            Pseudo Genes (multiple problems)  :: 31 of 111
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048355.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Raoultella ornithinolytica"
                     /mol_type="genomic DNA"
                     /strain="23"
                     /isolation_source="environment"
                     /db_xref="taxon:54291"
                     /plasmid="p23_F"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            join(<4340..<4340,1..83)
                     /locus_tag="GZS10_RS31480"
                     /pseudo=""
     CDS             join(<4340..<4340,1..83)
                     /locus_tag="GZS10_RS31480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(242..1024)
                     /locus_tag="GZS10_RS30780"
                     /old_locus_tag="GZS10_30780"
     CDS             complement(242..1024)
                     /locus_tag="GZS10_RS30780"
                     /old_locus_tag="GZS10_30780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128319234.1"
                     /translation="MTECILFIFEGDKTEKTITDSLLKFYIKNDKRIVFSSFKTDIYAL
                     YKMLSEDEDLDVFAIIKERNPELNDYTRDSFSQLFLFFDYDGHSPAANDDKIIELLDFF
                     NEETENGKLFISYPMVESIKCLSNIDAIEEFCNHSVNICDCSGFKKYISEYAHKSLIHF
                     SLYDLVTWNSIIRVHCVKANFIIKGDMIFPTSYFNQQDVFSSQREKFILPNKVVSTLSS
                     FPLMLLDFFGHQNLFTRVSGVQIEDGDLLSSEEGQRTI"
                     /name="hypothetical protein"
     gene            complement(1017..2141)
                     /locus_tag="GZS10_RS30785"
                     /old_locus_tag="GZS10_30785"
     CDS             complement(1017..2141)
                     /locus_tag="GZS10_RS30785"
                     /old_locus_tag="GZS10_30785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640319.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_104897464.1"
                     /translation="MIYRFEVEGFKGFSEKLTLDLTHKKNYEFNQDCIFNGVVKKGMIY
                     GRNGIGKSNLGLALFDIVGHLTDFNINKNMYSNYINAVNVSGIAKFKYYFKFGDDFITY
                     SYGKRSIKEFIYEELVINDKVVARFDREKDSNAYFSLYGTETLNTDISESNISIINYIK
                     NNSILKDGLEKDLFFNFITYVDKMLYFRSLDSNSYLGLEVGSHFITPDIVERGNLVDFE
                     RFLNEAGVSCKLCVIKTQGNDGIVEDIGFDFGDKKIPFYGIASTGTKSLALFYYWMQRF
                     IDEKCVSFIFVDEFDAFYHHALSMLMVKKMKSVGAQVLLTTHNTNVITNELLRPDCYFI
                     MDHKGIEAIFEKTDKELREAHNIEKMYKAGAFND"
                     /name="ATP-binding protein"
     gene            complement(3339..3740)
                     /locus_tag="GZS10_RS30790"
                     /old_locus_tag="GZS10_30790"
     CDS             complement(3339..3740)
                     /locus_tag="GZS10_RS30790"
                     /old_locus_tag="GZS10_30790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3740..3931)
                     /locus_tag="GZS10_RS30795"
                     /old_locus_tag="GZS10_30795"
     CDS             complement(3740..3931)
                     /locus_tag="GZS10_RS30795"
                     /old_locus_tag="GZS10_30795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2..83
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="84.0"
                     /evalue="1.1e-21"
     Typing          4053..4340
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.772"
                     /coverage="90.0"
                     /evalue="1.06e-118"
ORIGIN
        1 gcgccttttc cggtaactat caacttgagt ccaacccgga aagacacgac gaagcgccac
       61 tggcagcagc cactggtaac tgatttagag gagttagtct tgaagttatg cgccagttaa
      121 ggctaaacta aacgaacagt tttggtgact gcgctcctct aactagccag ttacctcggt
      181 tcaaaaacct gcaagttatt tgaacctctg aaaaaaccgc cctgcaaggc ggtttttctt
      241 tttagatagt acgctgacct tcttctgaac tcaacagatc accatcttca atttgcactc
      301 cactaactcg agtgaataaa ttttgatgtc caaaaaaatc caacaacatc aacggaaatg
      361 agcttaaagt agaaactacc ttattcggta aaataaactt ttccctctga gatgaaaaca
      421 catcttgctg attaaaataa gatgtaggga atatcatgtc gcctttaata atgaagttgg
      481 cttttacgca gtgaactctt ataattgaat tccaagtgac gaggtcatac aaactgaaat
      541 gaatcaatga cttatgtgcg tactcggaaa tgtatttctt aaacccagag caatcacaaa
      601 tattcactga gtgattgcaa aattcttcaa ttgcatctat attagacaga cactttatag
      661 attcaaccat tgggtaacta ataaaaagct taccattttc tgtctcttca ttaaaaaaat
      721 caagcaactc aataatttta tcatcatttg cagctggtga gtggccatca taatcaaaaa
      781 acaaaaaaag ctgtgaaaat gaatctctcg tataatcgtt taactcagga tttctctcct
      841 taattatagc aaaaacatca agatcttcat cttcacttag cattttataa agagcgtata
      901 tatcagtttt aaaagatgaa aaaacaatcc tcttatcgtt ttttatataa aattttaaaa
      961 gactatctgt gatagtcttt tctgttttat cgccctcaaa aatgaaaagt atacactcag
     1021 tcattaaatg cacctgcttt atacattttc tctatattat gagcctccct tagttcctta
     1081 tctgtttttt cgaatatagc ctcaatgcct ttatgatcca taataaagta acagtctgga
     1141 cgaagaagtt cattggtaat aacatttgta ttgtgcgttg ttagtaaaac ctgagcgcct
     1201 acagatttca ttttcttaac cataagcatg gataaagcat gatgataaaa agcatcaaac
     1261 tcatcaacaa agatgaaaga aacacatttt tcatcgatga acctttgcat ccaatagtaa
     1321 aacaaagcca gtgacttagt ccctgtagat gctataccat agaaaggtat ttttttgtca
     1381 ccaaaatcaa acccaatatc ctcaacaata ccatcatttc cctgtgtttt aataacgcaa
     1441 agcttacaag aaacgccagc ctcattcaaa aacctttcaa aatcaacaag attacccctc
     1501 tctacgatat ctggggtaat aaagtgacta ccaacttcaa ggcccaaata gctattcgaa
     1561 tctagcgatc taaaataaag catcttatct acataagtta tgaagttaaa gaaaagatct
     1621 ttttccaatc catctttaag aattgagtta ttttttatat aattaattat agatatattt
     1681 gactccgata tatcggtgtt taaagtttca gttccataca atgaaaaata agcattagaa
     1741 tctttttctc tatcaaatct agctacaacc ttatcattta taacaagctc ctcataaatg
     1801 aactctttga tacttctttt tccatatgaa tatgttataa aatcatcgcc gaatttgaag
     1861 taatatttaa acttagcaat cccagaaaca ttaactgcat taatataatt actatacatg
     1921 tttttattaa tattaaaatc agttaaatgc cctacaatat cgaacaaagc aaggccaaga
     1981 ttgctctttc caataccatt cctcccgtaa atcattcctt tttttaccac accattaaag
     2041 atgcaatcct gattaaactc ataatttttt ttgtgagtaa gatctagcgt cagcttttct
     2101 gaaaaacctt taaaaccctc gacctcaaat ctgtatatca tgatattctc caataaagta
     2161 gcgtagaact atatcatggc accgtaaaaa aattacacca aataaaaaac attgatttta
     2221 tctatcacat aaattagtat tgtaactgaa ggctctagca gtcctatgaa gtgccgcatt
     2281 agtgctgcat tttataggtg tcagttccta aaactgacgg agactggaga gtatgaaatg
     2341 aaacgtaaaa gcgtttttgt gcatgcctac cgtcgctttc gctatggcga gtgggaagac
     2401 gtttgctgtc actaccgctc aatgcctagc taaacgacat ggctgaccgc gaagagcctt
     2461 caccccccct taactaaact ttccagtaaa gatgtaaaaa tcaataacca atactcacgc
     2521 gaatctcaac ggcatactta tctgtgtttt tcttgaacac ctatgagttt ttgtttcaat
     2581 cacagttttg ttacgattgc catttaacat aaagctataa ttgtacgcac cgctaaaacg
     2641 gcctcaggac gtttctgacg ggaaaccggt aaaacaggca acaaaccacc cgaaaacccg
     2701 ccagaaacgc gcaggctctg tttttacggc atgcatgagt atgcgcgcaa gtgcatagcg
     2761 cggaaattca cgccattcag ccagggtaag tcctgtttcc ggcgttcttc tgatccgaaa
     2821 acggtgaacg gggacgcaat ttattgcgtg gccgtgaggc gttgccggcg cagccggaaa
     2881 accggttttc agcacgttca ggaatcgggt tcgtcgacgg ccggcaatca cgttatggca
     2941 agttgccgga cgactcacca cacgattcac cggcgattcg aatcgtgaat tgaatcaaga
     3001 gtcaccatga acaaaaaacg cccaaattga tgaattgaat caagagtcga ttcatcataa
     3061 caacatgata ataaagcgtg attcacatat gaatcgtggg caattcgtaa acgattcgcc
     3121 atagagtcgt ccacgacttg gatctgcact ttgtaacaca tcactgcaca ttaatttttc
     3181 attaaccaat tgttttaaat aaggaatctg caaaatttta attttgcgta gtgtgtgggt
     3241 atttttagtc gtgaggagcg ttcagagatc tggcgatttt tgggaaggtt caggaactcc
     3301 ctgggatggc cggagaagaa aactcaagca tggcaggatc aggatttttt ccagaatttc
     3361 caccatggcg ccggagattc agttaatttt gcctggtgat tttcaagaag gagcatggcc
     3421 tgtttcaggc tgtcaatgtg cgaatccttg gacaaaagta aggtgttcag gtgttcaatc
     3481 tcctgtttca gatgacgaat cacctgttca ctctggtcag cagaggctgt atcacgctgt
     3541 ccagagacct gtccagaatg gtgttcaccc ttcggttgtt caaagggacc aaagacacgt
     3601 aaaagctctg aagtatctac catttttgca ttgcgttcac cgctacaggt ggacaggtca
     3661 cctgctttaa ttaacctgtg cagtgttgtt ctactcttac ccgtcagtcg ggcagcctca
     3721 ctaatagaca ctctggccat cagttaccat cctgtaatgc atccagtctc gaggacagtg
     3781 tacggaaaag atgctccgca tcatcgtgga gtttctcaca aaggtcggct tcgacatcga
     3841 gatcaagctc gttaagcttc tccagcagga gcagagactg attctggatg aatttagcca
     3901 tgttcagcgc tgttttctgt tgtttcgtca taagaggcct cagagcgtca gtgctcagtg
     3961 tagggatgtt ctcttttccg cttcctcgct cactcagtcg ctatgctcct gccgttcggc
     4021 tgcggcgagc ggcaggagca tgcgtataaa agctcgagaa aaccgagcgg tcgcgtaacg
     4081 gggaaaagga aaggccgcaa ggcggccctt ttttccatag gctccgcccc cctgacaagc
     4141 atcacagaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc
     4201 aggcgtttcc ccctggtggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
     4261 gtgtcattcc gctgttatgg ccgcgtttat ctcattccac gcctgacact cagttccggg
     4321 taggcagttc gctccaagct
//
