LOCUS       NZ_CP048354            10108 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Raoultella ornithinolytica strain 23 plasmid p23_E, complete
            sequence.
ACCESSION   NZ_CP048354
VERSION     NZ_CP048354.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951919
            Assembly: GCF_010365445.1
KEYWORDS    RefSeq.
SOURCE      Raoultella ornithinolytica
  ORGANISM  Raoultella ornithinolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Raoultella.
REFERENCE   1  (bases 1 to 10108)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10108)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: 23.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:32:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,190
            CDSs (total)                      :: 6,065
            Genes (coding)                    :: 5,954
            CDSs (with protein)               :: 5,954
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 38 of 111
            Pseudo Genes (incomplete)         :: 90 of 111
            Pseudo Genes (internal stop)      :: 21 of 111
            Pseudo Genes (multiple problems)  :: 31 of 111
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048354.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10108
                     /organism="Raoultella ornithinolytica"
                     /mol_type="genomic DNA"
                     /strain="23"
                     /isolation_source="environment"
                     /db_xref="taxon:54291"
                     /plasmid="p23_E"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            complement(453..668)
                     /locus_tag="GZS10_RS30715"
                     /old_locus_tag="GZS10_30715"
     CDS             complement(453..668)
                     /locus_tag="GZS10_RS30715"
                     /old_locus_tag="GZS10_30715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(675..1166)
                     /locus_tag="GZS10_RS30720"
                     /old_locus_tag="GZS10_30720"
     CDS             complement(675..1166)
                     /locus_tag="GZS10_RS30720"
                     /old_locus_tag="GZS10_30720"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032629998.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<1201..1905)
                     /locus_tag="GZS10_RS30725"
                     /old_locus_tag="GZS10_30725"
                     /pseudo=""
     CDS             complement(<1201..1905)
                     /locus_tag="GZS10_RS30725"
                     /old_locus_tag="GZS10_30725"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1844..2167)
                     /locus_tag="GZS10_RS30730"
                     /old_locus_tag="GZS10_30730"
     CDS             complement(1844..2167)
                     /locus_tag="GZS10_RS30730"
                     /old_locus_tag="GZS10_30730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_163063187.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2818..>2940
                     /locus_tag="GZS10_RS31475"
                     /pseudo=""
     CDS             2818..>2940
                     /locus_tag="GZS10_RS31475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(3260..3616)
                     /locus_tag="GZS10_RS30740"
                     /old_locus_tag="GZS10_30740"
     CDS             complement(3260..3616)
                     /locus_tag="GZS10_RS30740"
                     /old_locus_tag="GZS10_30740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(3819..4136)
                     /locus_tag="GZS10_RS30745"
                     /old_locus_tag="GZS10_30745"
     CDS             complement(3819..4136)
                     /locus_tag="GZS10_RS30745"
                     /old_locus_tag="GZS10_30745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4323..4565
                     /locus_tag="GZS10_RS30750"
                     /old_locus_tag="GZS10_30750"
     CDS             4323..4565
                     /locus_tag="GZS10_RS30750"
                     /old_locus_tag="GZS10_30750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            4894..6240
                     /locus_tag="GZS10_RS30755"
                     /old_locus_tag="GZS10_30755"
     CDS             4894..6240
                     /locus_tag="GZS10_RS30755"
                     /old_locus_tag="GZS10_30755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611902.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY-like element ISLad2 family transposase"
                     /protein_id="WP_076611902.1"
                     /translation="MSDKEIHRLPVIQAVCEKRLRRRDAASQLGISERQAQRLINRYRV
                     SGAEGLVSRKRGQPSNRRLTESLKLRVLTLIRENYSDFGPTLAAEKLRERHNIRLSIET
                     VRNWMTSDGLWVPHARRKSRVYQPRHRRDCLGELIQIDGSHHDWFEGRAPKCCLLVFID
                     DATGRLMHLRFSESETAFDYMLATREYIEQHGKPVSLYSDKHAIFRVSGPEKRNTTVTQ
                     FGRVLYDLAIELICANSSEAKGRVERANQTLQDRLIKEMRLEGITGIEAANAWLATFIA
                     DFNSRFSRPAKFPKDLHRPVQESPDELRDIFAWHDVRTVSKSLTFQYDKILYLMDTTEE
                     NSRLAGEKIKVLDYPDGTLAFLYGHRSLKCQAFDKLACVDQGQIVDNKRLGTVLRLAQV
                     KQDERESEGKRERSKKSPSRKAQVRIQEQLRAINPVLADPSLFVASSKR"
                     /name="ISNCY-like element ISLad2 family transposase"
     gene            6299..7102
                     /locus_tag="GZS10_RS30760"
                     /old_locus_tag="GZS10_30760"
     CDS             6299..7102
                     /locus_tag="GZS10_RS30760"
                     /old_locus_tag="GZS10_30760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000589340.1"
                     /translation="MIKFPTTKRVDLYKTAVSSEQLHLDLVAAQEFMFDAWENDDLEVV
                     LKLIRKAIKKSPLCADAYSFYCEISQEPPESKIGKLETALYAASIALGEDFQEFAGRFW
                     GFVETRPYMRAKAALAEALWESGNFYPAMAHSREMLKLNPNDNQGIRHLLANYYLELEM
                     VDDLALLLDDYPGDMRSFFQYTRALLAYRQSSPDADDIAKAAIDSNRHIPGLLSKCRLQ
                     IKSNSGYITLGGMDEAIYYVNHNIKPWIRTSGAIDWIVNNSLSKI"
                     /name="hypothetical protein"
     gene            7116..8477
                     /locus_tag="GZS10_RS30765"
                     /old_locus_tag="GZS10_30765"
     CDS             7116..8477
                     /locus_tag="GZS10_RS30765"
                     /old_locus_tag="GZS10_30765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="WP_000482601.1"
                     /translation="MFNLIMGGEPDYFEHWPMYERVSGSCDFPISRMLEGTSDDIRLKL
                     TPLNDKALSYIEKLPTLFMSELYSRDNVEYITLRLGVISNLRTVNKNVEFDFRITHSQD
                     DVVVINKELYQTALELGAYGLKRTHWGIKARDLNQTLALLNITTRSTPLPPTEALPDEV
                     DNYPIIDNVQSFMARVLEQDHEEDAEIFYRGHSDVSYELAPSVFRKNKKGNFKHLHSES
                     NLVREALTARPTEFVDDKTMLDKLVRMQHYGLPTRLLDITSNPLIALYFACCDISNNEN
                     TNEVDGHVIIFKTKRDRIKFFDSDTVSCISNISMLSQTLKDQLDCKMDKEAFNKTEACQ
                     KLIHYIKDEKPYFKDVIIPSDLERLIFVKGRNNNERMSSQSGAFLLFGNNAVYPDLVSN
                     PDDAMQEFKVEKIVIRNKARILKELARLNITDATVYQGMERTMKLIAAKFSAGD"
                     /name="FRG domain-containing protein"
     gene            8630..9070
                     /locus_tag="GZS10_RS30770"
                     /old_locus_tag="GZS10_30770"
     CDS             8630..9070
                     /locus_tag="GZS10_RS30770"
                     /old_locus_tag="GZS10_30770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016239970.1"
                     /translation="MNPITLSEMSDEDEEVKIISSYEPQDDEIAIVFDSSKITGGNALD
                     FFQFTKQSHSNVKVYGDSNGVSYLQLNSITLALGTFFATSVMIPFLVNLVSNYIQKKID
                     NFGTRDIDVKVSIIKKNKSGKHVKYTVSGKVDDVLKVINKIK"
                     /name="hypothetical protein"
     gene            9088..9894
                     /locus_tag="GZS10_RS30775"
                     /old_locus_tag="GZS10_30775"
     CDS             9088..9894
                     /locus_tag="GZS10_RS30775"
                     /old_locus_tag="GZS10_30775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016239971.1"
                     /translation="MNIHEKLKRWMCITQEDSAILDYLNAELKKAQSLSLNNESNRLFL
                     YKTILLAHLKYIQVINLLTRGDFYEAWVELERIEIDLIHIKENNEFLPEVNFYGVNFLA
                     RMVCNWQALFPYKIFGSSREIIKEVKCSVCNTTRSFINDCGHVKNKLYNGVLCFDEVID
                     FELITYDIVSNPVNKCSVFFSNDGDHYNYSTLISVVKYIQSPHQIFNITTWRFKAKEHD
                     GVLSPENICPCGDSLKKYADCCLPRNGIYKKHIDIWFPFPLNVEPI"
                     /name="hypothetical protein"
     Typing          complement(2283..2456)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          2380..2927
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.559"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(362..1854)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.2"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1851..2167)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.59e-59"
ORIGIN
        1 gctgaaacgg ggttggctgc gtttccggtg ccagacgcga gaaagtggcg acagacataa
       61 cgaaaaaaca ccagaaacgc agccgctgaa tattacactg gtgcatagtc atgcagccag
      121 atgcatggat gttaaagtca cactaaaatc cgcgccaatg ccttaaaaac cgacaaatgc
      181 ttctccagcc agtcccacag ctccgccgcc agatacaggc agttaagcaa tgattcaatg
      241 agatttaaca gcttcatttc ccgctcctga ataagtcgtg acctttttga tcacgaacct
      301 atttagcggg agaaataaga gcccctcttc gtgcgggttg tcagctgcct ggatggttta
      361 aagtgtcggc ccgcggtagg ttttctgcgg ctggcgctcc agctccagtg ttttctgata
      421 ctcattctcc ctctgccgga gctcgatggc attcagttcc cggacaagcg gcgcagctgc
      481 tcgcttaaac tcagcacctg cgtgctcaag tccgacacgc gctcgttcag caccgcgttc
      541 tcccgttgca taagcccaca catgctccgc cattccgcga aggcgctctc ccattcgtcc
      601 agcctttgcg agtagtcctg ctgtagctgc tctaatgcgc tcagcaactg cttttccagc
      661 tctgtcattt tccgctactc ctgtttcagg cggatccatg tcgtcccgtc gtaggggatg
      721 gcttcggttt gcatcccggg cggcaggata agaaacctcc ggccgtcgct gctctcctga
      781 tagcgcactc cccacgtccg ggcagtcatt ttcgccaggg tatcttcctg ctgacgaaga
      841 tgcgtgtaat tgtcggttat ctgctgcccc tgccaccaca gtatgctccc gctcgtgccg
      901 atcagcagcg ctgacaccat caggatggtg aaccatgtcc gcccggcagt ccgcagtacg
      961 ctccgccggt ttttctccat cgcctcgctc atactctgct cgtgtgcgag gatggcgtcg
     1021 ctgattctcc tcgcgcttgc gctcagttcc gctctgacag actgctcgtg ctcgctgaat
     1081 gcggctttca gcatctcgcc ggtactctgc tgctgcgctt tcgattgctg ctctaagtcc
     1141 ttcgccagcg ttaaaagact gctcatagat ggctcccttc agtcggatgt ttcgcccccc
     1201 gtccgggtcg gcaatgctga tactgctttt cgtggtgcgc accacctcaa aacctgaccg
     1261 ttccagcgcc tcagtgacgt cctgacggtt tttcagctcc ccggacgaag caagggtcag
     1321 cagtccgcgc gtgatggctt cagtggcctc ctgcttcgtt ttcggcagtc cggacggcgt
     1381 gaccagcgcc cggcggtttt ccggcgcgtt cgggtcatgc agccccagtc tgccgttcac
     1441 gatggtctgc caggcatcga tgcgcggccg gtcggcgcgg tcgtagtacg gctggagccg
     1501 cctgccggtc agcagttcgg tattggggat cagaaagttc agctccagac gccccttgtc
     1561 cgcgtgctcc acccacagaa tgctgtactg atctttatca agtccgggca tcagaacccg
     1621 ctcgaagctg gccatcagtt tttcccgctg gccgggaggc agatcggcct ccgcaaacga
     1681 cagtacgccg gaggtgtatt tcttcacgta aggcgacgcg tcaatcagct cccggacttc
     1741 ttccggcttc ccctgcagga cgctggcgcc ttcacgctgc cggtctttac ccagcagata
     1801 gtccaccggc ccggcgccac cgccgcgacc gcgtggatga aatttaacga tcatcgtccg
     1861 ttcctttctc aagaacggcg tgccgcagcc gctcaagccc ggcatcgatg gccatcagcg
     1921 ccgccacaat ctgtaccctg tcatggccgg tgccgccgcc ggcgttaaca cggcgggcta
     1981 tctggttcag gttattgccc ataccggcaa gctggcgcag cagcgccggc gagagtgagg
     2041 ggagctttcc ggtgcgcgca ggcttttcgt ccaggcaggt ctgccgcatc cacgccgcca
     2101 gcggcctgcc gtcgcagcgc tcaagcagcc gccggtgctc gccctccgtc acccacatgg
     2161 tgagcatctt gttccgtttc tctgacaaac cggcctcctg ataacgccgg gacgggcggg
     2221 aaagcctttc ccggtcggcg cggggttgga caagccgcag gcgcgtcagt ggctgttagc
     2281 gggtttcggg gcgcagccct gaaccagtca cggagcgcta gcggagtgta tactggctta
     2341 ctatgttggc acggatacgg aaatcagtga agtgcttcat gtggcaggag aaaaaaggcg
     2401 gcaacggcgc gtcagcagaa tatgcgatac aggatatatt ccgcttcctc gctcactgac
     2461 tcgctgcgct cggtcgttcg gctgcggcga gcggtgcggg cttacgaacg gggcggaaat
     2521 ttcctggaag atgccaggaa gaaacttaac agggaagcga taaggccacg gcagagccgg
     2581 ttttccatag gctccgcccc cctgacgagc atcacagaat ctgacgctca aatcagtggt
     2641 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctgatggc tccctcgtgc
     2701 gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt
     2761 ctcattccac gcctgacact cggttccggg taggcagttc gctccaagct ggactgtatg
     2821 cacgaacccc ccgttcagtc cgaccgctgc gcctgttccg gtaactatcg acttgagtcc
     2881 aacccggaaa gacacgacaa aacgccactg gcagcagcca ctggtaacag gattagcaga
     2941 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3001 ggaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccaggtt aagcagttcc
     3061 ccaactgact taaccttcga aaaaccgcct ccccaggcgg ttttttcgtt tacagagcaa
     3121 gagattacgc gtacagagca cggatctcaa gaagatcgta agaagaaagt taacatgagc
     3181 gtttttatcc ctcaaaaaga aggcttctag ttatagaagc cttctttttg agggataact
     3241 tatatttttt taaaaaaaat catgcaatag catgtttctt ctcagaacta tcaacaatgt
     3301 gtttcctttc tatgatttca tgagcgggaa tcactctgtc tatttcttct tgcaatcgag
     3361 ggctactagt aacctcccat aaatcaacag actgctgtat atttagccag aactttgcag
     3421 tggtatcgaa ggctttagcc aacctaaaag ccatatcggt agacagcttt ctattattgt
     3481 taactaaagc actggcagag tttctatgca cattcaacat ttcagccaag tcattgattt
     3541 ttaaaccact tggttcgaga tattcataaa gaataatatc ccctacagtt gatggttttc
     3601 tagttgcttg ggacatgctt tactcctaaa ttttttactt ctctctgtta gatgaggaag
     3661 ataagtattt cgtctattac cagctgtagc gatgagtaat tatcttaaat atagtcacgt
     3721 catttttttt attaatcatt atttgtaatg acattttaat gtaggtgagg gaacgcctca
     3781 cctacttgtt ttataagata acagccacga cctgttatct atcggtaatc atgtggatcg
     3841 agatacaaat cctcgactcc gttttcaccc cacttaaata tcaagcggta cttctcattc
     3901 acccgtatgg atgaataccc tgtcaatgga gggttgagat tctcaaatcg gtttcccggg
     3961 ggagaccgca aatctccaac tttatcagca gcattcagca tatccagctt gcgacctaat
     4021 gaagcactaa tcgcagttgg aatctttcga ttggctttac catatttgta aaactggtat
     4081 agccaatcgt ctcgaaactg aatgcggcaa tcatcatgtt catgatcttg ctgcattttt
     4141 cttttacctc aatttttgcc gcaacttggc aattttttcg atcaccttct tacctccgat
     4201 atagtacagc aaacatatca aatgcacaac agcacagtgc acttgtgcaa tcaatggcta
     4261 tgctgtagaa tgcactcatg cacagtgcag gcgtgcagaa taaatgataa tttggaggtt
     4321 gcatggctct tgttcgctca ggaaacaaaa gcaagatttt tgaagcagat acgaagttta
     4381 actcggtttt tgacactgaa tataagccgg gagaaacatg tgaatatgaa gggatttata
     4441 aatgtaccgg ttgcggtaaa gaagtaacta tcgcaggcgg gaaaacaatc ccaccacaaa
     4501 accaccacca acataccccc gcgcaaggga acattcgttg gcaattgttt gtttatgctc
     4561 agtgaggtat agaaaatggc agatcgtcga ccactcaacc cacacgttgt cgttcctaaa
     4621 ccaggcggcg gaactcaacc tcgtgaccgt aacaatgacg gaagggtcag aggaaaacgc
     4681 tcagatgcag gaaagccacg aaaataacag caagccgcca gcctggcggc ttttttatat
     4741 ctgcagctga tactgattta ccataacatc cgttgggcgc accgctgatg ttagaaccat
     4801 ttagtaatgt cactttttat ccagatagaa acgtcacctt agagtaagct aaactgaatg
     4861 caattctaag gggctcaacg catgttggtg accatgtcag ataaagagat ccaccgccta
     4921 ccggtgatac aggcagtttg tgaaaagcgt ctacgccgtc gtgatgcagc atcacagctt
     4981 ggcatttcag aacgtcaggc gcaacgactt atcaatcgtt accgagtttc tggtgctgaa
     5041 ggacttgtca gccgcaaacg cggccagccc agcaaccgcc ggttgaccga atctctcaaa
     5101 ctacgagtac tcacgctgat acgtgaaaac tacagtgact ttgggcccac actggccgca
     5161 gaaaaactca gagagcgcca taatatccgg ctttcaatcg agaccgtccg caactggatg
     5221 acgtctgacg gactctgggt cccgcatgct cgccgtaagt cacgggttta tcaaccccga
     5281 catcggcgtg attgtctggg tgaacttatc cagatcgacg gctcccacca tgactggttt
     5341 gaaggccgtg cgccaaaatg ctgcctgctg gtctttatcg atgatgccac cgggcgtctg
     5401 atgcatttgc ggttcagcga gtcagaaaca gcctttgact acatgctggc aacccgggaa
     5461 tacatcgagc agcatgggaa gccggtatcg ctgtacagtg acaaacacgc aatatttcgc
     5521 gtcagtggtc cggagaaacg caataccacc gttacccagt tcggacgtgt gctttatgac
     5581 ctggcaatcg agcttatttg tgccaacagc tctgaagcta aaggccgcgt ggaacgtgcg
     5641 aatcagacac ttcaggatcg gctgatcaaa gaaatgcgtc tggaaggtat aaccggcatt
     5701 gaggccgcaa atgcctggct tgccactttc atcgctgact ttaacagccg gttttcacga
     5761 ccggctaagt tccccaaaga tttgcatcgt ccggtgcagg aaagccccga tgaattaaga
     5821 gacatctttg cctggcatga tgttcgtacc gtgtcgaaat cactgacctt ccagtacgat
     5881 aagatccttt atctcatgga caccacagaa gaaaatagcc gtcttgccgg tgaaaaaatt
     5941 aaagtactgg attatcccga tggcaccctc gccttcctct atgggcaccg gagccttaag
     6001 tgccaggcat ttgataagct ggcctgtgtc gaccaggggc agattgttga taacaaacgt
     6061 ctcgggactg tactccgact ggctcaggta aaacaggatg agcgggaaag cgaaggcaaa
     6121 cgggaacgaa gcaaaaaatc gcccagccgt aaggctcagg tgcgcatcca ggaacagctc
     6181 cgggctatca atcccgttct ggcagacccg agcttgtttg tggcaagttc aaagcgatag
     6241 aatcgaccat cagtgctgca aagtaccgga acagtgagtc cccaaaagga tacttgagat
     6301 gattaaattc ccgactacaa agcgcgttga tttgtataaa actgctgtca gctctgagca
     6361 attacatcta gatctggttg cggctcagga gttcatgttc gatgcctggg aaaatgatga
     6421 cctggaagtt gtgctgaaac tcatcagaaa agctatcaag aaatccccgc tctgtgctga
     6481 cgcatactca ttctactgcg agatatctca agaacctcct gagtcaaaaa tcgggaagct
     6541 ggagactgct ttgtacgcag ccagcattgc acttggcgag gattttcagg agttcgcagg
     6601 acgtttctgg ggatttgtcg aaacacgtcc atatatgaga gcaaaagcag ctttggctga
     6661 ggccttatgg gagtctggta atttttatcc ggctatggct catagccgcg aaatgctgaa
     6721 gctcaacccg aacgataacc aggggattcg tcatttatta gcgaactatt accttgagct
     6781 tgaaatggtg gatgatctgg ccttgctact ggacgattat cctggggata tgcgttcgtt
     6841 ttttcagtat acacgcgccc tactcgccta ccgacagtca tccccggatg ccgatgatat
     6901 cgctaaagct gcgattgatt ctaatagaca catccccggc ttgttatcga agtgcagatt
     6961 acagatcaag tctaacagtg gatatatcac gttaggtgga atggatgaag ccatttatta
     7021 tgtgaatcat aatataaagc cttggattcg cacctctggc gcaattgatt ggattgtaaa
     7081 taattcatta agtaaaatat aaggatttag ttttaatgtt taatctaatt atgggcggag
     7141 aacctgacta ttttgaacat tggcccatgt atgaacgggt tagtgggagt tgtgattttc
     7201 ctatatcacg tatgttagaa ggcacctctg atgacatccg gttgaagtta acgcctttga
     7261 atgataaagc attatcttat attgagaaat tacctacgct ttttatgtca gaactttatt
     7321 cccgagacaa tgttgaatac attactttgc gacttggtgt aatttcaaat ctacgaacag
     7381 ttaacaaaaa tgttgagttt gatttcagaa tcactcactc acaagatgat gtcgtagtga
     7441 taaacaaaga gttatatcaa acagctttag aattaggtgc atatgggctg aaacggactc
     7501 actggggaat aaaagcacgt gatttaaatc aaacattagc acttctaaat ataacgacaa
     7561 gaagtacacc acttcctcct actgaagcac ttcctgatga agttgataac tacccaataa
     7621 tagataatgt tcaaagtttc atggcgcgtg ttttagaaca agatcatgaa gaagatgcag
     7681 aaatatttta tcgtggacat tctgatgtat cttacgagtt ggctccatca gttttcagga
     7741 aaaacaaaaa aggtaacttc aagcatttgc acagtgaatc aaatttagtc cgtgaagccc
     7801 taacagcaag gccaactgag tttgttgatg ataaaacaat gctagacaag ttagtaagga
     7861 tgcaacacta cggactacca actcgtttgt tggatataac atcaaatcca ctaatcgctc
     7921 tgtatttcgc ttgttgcgac attagtaata atgaaaatac aaatgaggtt gatggacatg
     7981 ttattatttt caaaacaaaa agggatcgta taaaattctt tgactctgat acagtaagtt
     8041 gcatatcaaa tatatcaatg ctaagtcaaa cactgaaaga tcagctggat tgtaaaatgg
     8101 acaaggaggc tttcaataaa actgaagctt gtcaaaaact catacattac attaaagatg
     8161 aaaaacctta ttttaaagat gtaattatac catcagattt ggaaaggtta attttcgtta
     8221 aaggaagaaa taataatgaa aggatgtcat cacagtcagg tgcattctta ttatttggga
     8281 ataatgctgt ttatcccgat ttagtttcaa accctgatga tgctatgcaa gagtttaaag
     8341 tagaaaaaat agtgatcaga aacaaagcta gaatacttaa ggaactcgca aggttaaaca
     8401 ttacagatgc gacagtttat caaggcatgg aaaggacaat gaaactaatt gcggccaaat
     8461 tttccgcagg agattgatct tcatgtggtt ttttgacaca ctagcctgtc gctactggca
     8521 ttggtccctt gtatgctaag catatgaact ctggtgtgtg ttggacagga gcaattaacc
     8581 agtcgtatag taaaatttga atgtaaaaat ataaaacaag gagttgagaa tgaaccccat
     8641 tactttgagt gaaatgagtg atgaagatga agaggtaaaa atcatttctt catatgaacc
     8701 tcaagatgat gagattgcga tcgtttttga tagttcaaaa attactggag gtaatgcact
     8761 ggattttttc cagttcacca agcaatcaca ttctaatgtt aaagtgtacg gagattctaa
     8821 tggtgtttcg tacctgcaat taaacagtat aacgttagcg ttaggcactt tctttgccac
     8881 atcagtaatg ataccttttt tagtaaatct tgtttcaaat tatattcaga aaaaaattga
     8941 taactttgga actagagata tagacgttaa agtctctata attaaaaaaa ataaatcagg
     9001 aaaacatgtg aaatacactg tttctggaaa agtagacgat gttttaaaag taattaacaa
     9061 aatcaaataa aaatctatat agatattatg aatattcacg aaaagttaaa gcgttggatg
     9121 tgtattactc aagaagatag tgctattctt gattatctta atgctgagtt aaaaaaagct
     9181 caatccttga gcttgaataa tgaatcaaac agattgtttt tatacaaaac aattttacta
     9241 gcccatttaa aatatattca agtcattaat ttacttacaa gaggtgattt ttatgaagca
     9301 tgggtagaac ttgaaaggat agaaatagat ttaatacata ttaaagaaaa taatgagttt
     9361 ctgcctgagg ttaatttcta tggtgttaac ttcttagcga gaatggtatg taattggcag
     9421 gcgttattcc catataaaat cttcggtagt tctcgtgaaa taataaaaga agtgaaatgt
     9481 tcagtatgta atacaacccg tagttttatt aatgattgtg gtcatgttaa aaataaactt
     9541 tataatggag ttctttgctt tgatgaagtg atcgattttg aattaattac ttatgacata
     9601 gttagtaatc ccgttaacaa atgtagtgtt tttttctcca atgatggtga tcattataac
     9661 tattcaactt taattagtgt tgtcaaatac atacaatccc cacatcaaat tttcaatatt
     9721 acgacctgga gatttaaggc gaaagaacat gatggagtat tatcgccaga aaatatttgt
     9781 ccatgtggag attctttaaa aaaatatgca gattgttgtt tgccacgtaa tggtatttat
     9841 aaaaaacata tagatatttg gtttcccttc ccgttgaatg tagaacctat ttagaaccag
     9901 atattcaata actaaagatc ccgttgacac agatatttcc atattaagat gttttcgtta
     9961 tatttttatt ccagtctttc atatttaact tttgctttat taattggttg agtgggataa
    10021 cgcagcagtc acattaatat gattacatag gctgaaagtg ccctttctat cttgcacaaa
    10081 gaggtgacat tttaaacttg ctttgaca
//
