LOCUS       NZ_CP048348             2088 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain 124 plasmid p124_D, complete sequence.
ACCESSION   NZ_CP048348
VERSION     NZ_CP048348.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951920
            Assembly: GCF_010365465.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: 124.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010365465.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:51:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,807
            CDSs (total)                      :: 4,692
            Genes (coding)                    :: 4,414
            CDSs (with protein)               :: 4,414
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 278
            CDSs (without protein)            :: 278
            Pseudo Genes (ambiguous residues) :: 0 of 278
            Pseudo Genes (frameshifted)       :: 100 of 278
            Pseudo Genes (incomplete)         :: 178 of 278
            Pseudo Genes (internal stop)      :: 53 of 278
            Pseudo Genes (multiple problems)  :: 44 of 278
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048348.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="124"
                     /isolation_source="environment"
                     /db_xref="taxon:562"
                     /plasmid="p124_D"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            305..1270
                     /locus_tag="GZS11_RS23790"
                     /old_locus_tag="GZS11_23790"
     CDS             305..1270
                     /locus_tag="GZS11_RS23790"
                     /old_locus_tag="GZS11_23790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_039064478.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLAIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            1274..1540
                     /locus_tag="GZS11_RS23795"
                     /old_locus_tag="GZS11_23795"
     CDS             1274..1540
                     /locus_tag="GZS11_RS23795"
                     /old_locus_tag="GZS11_23795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          699..930
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 ggttattttt tgcgcaattc tcgcgcgtga tccttgtatt tatacttaag ggataaatgg
       61 cgggtatgaa atagtggttt agcccagtaa tgacgaggct ttgagtgggt tttgacaggt
      121 caaagaaaat ggagcagaat tgaggcgttt ttaatcggcg ttggggagtg cgtcaacact
      181 ccccaacatt tcgaatgtgt cacctcagcg gcaaactctg gtgacatgta ctggctcgca
      241 atgcacaggt acgtgatgaa tataccacat caaatcacag cctgcccaga tcggagcagg
      301 cttaatgtca gaagataaat tcctttcgga ctacagcccc cgtgatgcag tttgggatac
      361 ccagcgcacg cttaccgatt ctgtcggggg tatctaccag actgctgctg aattcgagcg
      421 ctatgcactc cgtatggcct cctgtagcgg tttgttacgt tttggttggt ctaccatcat
      481 ggaaaccgga gaaacgcgcc tacggcttcg tagtgcgcaa ttttgccgtg tccgtcattg
      541 ccctgtctgc cagtggagaa gaaccctcat gtggcaagcc cgtttttatc aggctctacc
      601 gaaaatcgtt gtggattacc cgtcttcccg atggttgttt ctgacgttaa ctgtcaggaa
      661 ctgcgagata ggtgaacttg gaacagtcct tacagcaatg aatgcggcgt ttaagcgaat
      721 ggaaaagcga aaggagctat cacctgttca ggggtggatc agggctacgg aggtgacgcg
      781 aggtaaggat ggcagcgcac atccgcattt tcactgtctg ctgatggtgc aaccttcttg
      841 gtttaaaggg aagaactacg ttaagcacga acgttgggta gaactctggc gcgattgctt
      901 gcgggtgaac tatgagccga atatcgatat tcgggcagta aaaactaaga caggtgaggt
      961 tgtggccaac gttgccgagc aactgcaaag cgcggttgct gaaacgctga aatactccgt
     1021 taaaccggaa gatatggcaa acgatcctga gtggtttctt gagctgacgc ggcagcttca
     1081 caagcgccgt tttatctcga ccggtggagc gctaaaaaac gtcctccagt tggatcgaga
     1141 aaccaatgag gatcttgcca ttgccgacga tgtaggggat ggcactgatg acgggaagcg
     1201 gacggcgttt gtctgggatt caggtaaacg gcgttacaaa cgcgcccctg agaaggataa
     1261 atcggattaa cgtatgaata ttaatattga atacctgaat ggaaataaga ctattggttt
     1321 atttttttta agaagtgaag cggtgattcc tgacaggttt aaaaacctta ttttgcttat
     1381 tgatggatta agttttggca catttggttt tcatccgcac gaaggttttg aggatgaatt
     1441 aattttatat attcagaaaa caaacgagag ggtaaaaact ctttttgtga aaatagattt
     1501 aaacggtatc aagtttggat ttttaagaac gcattcttag ttctggaaaa gagccagcgg
     1561 caggctgagg tgataggtac gagattgcat gcaatctcta gtgctctgtc tatcctgcat
     1621 tatcctcagc attatcctca gccttgccaa ctcgacacca atgcaggata gacaatccga
     1681 tgtcaaatgt taacactctg cgagtggtac attttccccg gattatcgtc ctgagcctgc
     1741 cgctggctct ctttctaccg cctcgctttg ctcgttgctc aacgcctcac agacacggat
     1801 taaaatccgc atccgttcac cgttttttaa agtccgttaa aagcatgatg ccatctccga
     1861 gagttaatct cgtcaaatgc taaatcgtgg gggtcccctt tggggttccg atttagtgat
     1921 tgacgacacc accgattaaa aaacttatgc ggggtggatg gtttcacgaa gtgaggccat
     1981 ccacctgtaa gacagggttt tgtttttatc ccctgttttg gtgatcgggt gtgtggaaaa
     2041 ggttggggta agccgttcgg ggtgcttgtt ttggggggtt aaaattgt
//
