LOCUS       NZ_CP048347            28663 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain 124 plasmid p124_C, complete sequence.
ACCESSION   NZ_CP048347
VERSION     NZ_CP048347.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951920
            Assembly: GCF_010365465.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28663)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 28663)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: 124.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010365465.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:51:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,807
            CDSs (total)                      :: 4,692
            Genes (coding)                    :: 4,414
            CDSs (with protein)               :: 4,414
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 278
            CDSs (without protein)            :: 278
            Pseudo Genes (ambiguous residues) :: 0 of 278
            Pseudo Genes (frameshifted)       :: 100 of 278
            Pseudo Genes (incomplete)         :: 178 of 278
            Pseudo Genes (internal stop)      :: 53 of 278
            Pseudo Genes (multiple problems)  :: 44 of 278
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048347.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..28663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="124"
                     /isolation_source="environment"
                     /db_xref="taxon:562"
                     /plasmid="p124_C"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            1..858
                     /gene="repA"
                     /locus_tag="GZS11_RS23595"
                     /old_locus_tag="GZS11_23595"
     CDS             1..858
                     /gene="repA"
                     /locus_tag="GZS11_RS23595"
                     /old_locus_tag="GZS11_23595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator RepA"
                     /protein_id="WP_122990725.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSRF
                     DFAIHVAHARSKGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECGLA
                     TESAAGKLSITRAARALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDVSEE
                     AVAAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIKRARA
                     RRDAGRERQDIVTLVKRQLTREIAEGRFTASREAVKREVERRVKERMILSRNRNYSRLA
                     TASP"
                     /name="repA"
     gene            1774..2043
                     /gene="yacA"
                     /locus_tag="GZS11_RS23605"
                     /old_locus_tag="GZS11_23605"
     CDS             1774..2043
                     /gene="yacA"
                     /locus_tag="GZS11_RS23605"
                     /old_locus_tag="GZS11_23605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="WP_000079930.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQH
                     NTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVADK"
                     /name="yacA"
     gene            2043..2321
                     /gene="yacB"
                     /locus_tag="GZS11_RS23610"
                     /old_locus_tag="GZS11_23610"
     CDS             2043..2321
                     /gene="yacB"
                     /locus_tag="GZS11_RS23610"
                     /old_locus_tag="GZS11_23610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="WP_001384452.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQ
                     PYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
                     /name="yacB"
     gene            complement(2680..>3282)
                     /locus_tag="GZS11_RS23615"
                     /old_locus_tag="GZS11_23615"
                     /pseudo=""
     CDS             complement(2680..>3282)
                     /locus_tag="GZS11_RS23615"
                     /old_locus_tag="GZS11_23615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(<3263..3780)
                     /locus_tag="GZS11_RS23620"
                     /old_locus_tag="GZS11_23620"
                     /pseudo=""
     CDS             complement(<3263..3780)
                     /locus_tag="GZS11_RS23620"
                     /old_locus_tag="GZS11_23620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            <3872..4162
                     /locus_tag="GZS11_RS23625"
                     /old_locus_tag="GZS11_23625"
                     /pseudo=""
     CDS             <3872..4162
                     /locus_tag="GZS11_RS23625"
                     /old_locus_tag="GZS11_23625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612074.1"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /name="integrase core domain-containing protein"
     gene            4546..4734
                     /locus_tag="GZS11_RS23630"
                     /old_locus_tag="GZS11_23630"
     CDS             4546..4734
                     /locus_tag="GZS11_RS23630"
                     /old_locus_tag="GZS11_23630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001051770.1"
                     /translation="MAKGESGRIVLEVEPELKKALYSVLAMEQKTLKDWFVDKAQEHIC
                     EKKSELIERFSKVDNEI"
                     /name="hypothetical protein"
     gene            4724..6349
                     /locus_tag="GZS11_RS23635"
                     /old_locus_tag="GZS11_23635"
     CDS             4724..6349
                     /locus_tag="GZS11_RS23635"
                     /old_locus_tag="GZS11_23635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668952.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="WP_058905638.1"
                     /translation="MKFKADQTSQKLRGGYYTPQNLADYVTKWVLSKNPKTILEPSCGD
                     GVFIQAIANNGYNSNIELFCFELFDTEASKALERCKLNNFSNATITEGDFLVWANECLK
                     KNKQIFDGALGNPPFIRYQFLERNFQEQAQLVFEHLDLKFTKHTNAWVPFLLSSLALLK
                     QGGRIGMVIPSEISHVMHAQSLRSYLGHVCSKIVIIDPKEIWFEDTLQGAVILLAEKKQ
                     YPDEASQGVGIVSVSGFEFLQEDPNVLFNDTAGINGETVEGKWTKATLSIDELQLIKRV
                     IAHPDVRKFKDIAKVDVGIVTGANNYFLVDNETVKLYKLERFAHPMFGRSQHCPGIIYD
                     EKQHVENQKKGLPTNFLYIDEEFEYLSKSVKNYIKLGEVEEYHKRYKCRIRKPWFKVPS
                     VYSTEIGMLKRCHDAPRLIHNRVRAYTTDTAYRVSSTVTSTENLVCSFLNPITVITAEL
                     EGRFYGGGVLELVPSEIEKLYIPIVEGLEHNVEELNLLIKDGQIERVIRQQGSLILDKL
                     GFTQEENEKLVEIWKKLRDRRLRK"
                     /name="N-6 DNA methylase"
     gene            complement(6419..9415)
                     /locus_tag="GZS11_RS23640"
                     /old_locus_tag="GZS11_23640"
     CDS             complement(6419..9415)
                     /locus_tag="GZS11_RS23640"
                     /old_locus_tag="GZS11_23640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034305.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="WP_058905639.1"
                     /translation="MNKKDQLQRLIDKYKGDIDYYRSARYNETQLRTDFLDQLFLILGW
                     DITNAAGKPTNEREVLVEEGLKARAGENTKKPDYTFRLFSERKFFLEAKKPSIDISTTI
                     EPALQVRRYGFTAKLKISVLSNFEYTAIYDCSNQVKETDSVANSRIKLYHFTELVDKFD
                     EINNLIGRESVYTGHFDNEWSEIENKILRFSVDDLFLKQINDWRLLLANEFLQIKNELP
                     EEKLNDLVQNYINSIVFLRVCEDRDLEEYETLYHFAQDKDFQSLVKKLKSSDKKYNSGL
                     FSLEYIDELLSNACSCIWSIIEQLYFPQSTYSFSVFSSDILGNIYEIFLSEKVRIDELG
                     NVTIQPKEEHIDRDVVTTPTHIVKEIIRNTVVEYCKGKSDIEILNSKFADIACGSGAFI
                     IEAFQFIQDILIDYYIQNDKSKLQQISEHTYKLKFEVKREILCKCIYGIDKDYNATKAC
                     TFGLLLKLLEGETTETIGKDTPILPALDTNILFGNSLIDSGDKVKQEDIFSINPFDLTN
                     YQFDVIVGNPPYMATEHMNQLTPKELDIYKRKYKSAYKQFDKYFLFIERSIQILKDHGY
                     LGYILPSRFIKVDAGKKLRKFLSENKYLSKLISFGSYQVFKNKTTYTCLLFLNKENHDN
                     FSFYEVKDFKKWLTREDKYLLSSTYQTSSLDSDTWILEKKINDILKLMFSKSEQLGNIV
                     GKSNVANGIQTSANKYYIHKEIKSENGFIYFEYDGIEYHIEKELTRPYFETNRSGDDSF
                     YTYKDVEPNSFVVYPYKKVGERIQFIEYDELKRQYPKLFEFLQVVKVHLNDKKRSIKPD
                     PTGPNEWYRYGRSQALENCDVDQKLIVGILSNGYKYSIDNHRTFVSSGGTAGYSIINVP
                     NNVRYSIYYIQAILTSKYLEWFASIYGDIFRGRFVARGTKVQTRMPIPTIDFDDPKQKE
                     IHDTISSKQQYLNKLYSQAQKSADRDKIIFERQFEQEKIQMDYLIKNLFDLGDLDSEIP
                     TVEDLYKNL"
                     /name="N-6 DNA methylase"
     gene            complement(<9769..9969)
                     /locus_tag="GZS11_RS23645"
                     /old_locus_tag="GZS11_23645"
                     /pseudo=""
     CDS             complement(<9769..9969)
                     /locus_tag="GZS11_RS23645"
                     /old_locus_tag="GZS11_23645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012304633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /name="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(9966..>10514)
                     /locus_tag="GZS11_RS23650"
                     /old_locus_tag="GZS11_23650"
                     /pseudo=""
     CDS             complement(9966..>10514)
                     /locus_tag="GZS11_RS23650"
                     /old_locus_tag="GZS11_23650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(10555..>11346)
                     /locus_tag="GZS11_RS23655"
                     /old_locus_tag="GZS11_23655"
                     /pseudo=""
     CDS             complement(10555..>11346)
                     /locus_tag="GZS11_RS23655"
                     /old_locus_tag="GZS11_23655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS629 family transposase"
                     /name="IS3-like element IS629 family transposase"
     gene            complement(11384..12997)
                     /locus_tag="GZS11_RS23660"
                     /old_locus_tag="GZS11_23660"
     CDS             complement(11384..12997)
                     /locus_tag="GZS11_RS23660"
                     /old_locus_tag="GZS11_23660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="WP_162676772.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSALT
                     RAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVDLAEALLKQREQDSDRYSGR
                     EDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLELVS
                     SALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPLYRQS
                     EIFARQGVELSRALLSNWVDACCQLMTPVNDALYRYVMNTRKVHTDDTPVKVLAPGQKK
                     AKTGRISTYVRDDRNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAFTGYDRL
                     FSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEIRGLPESE
                     RLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYSDDGLAEAD
                     NNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRHILSVLP
                     EWPSNRVDELLPWNVVLTNK"
                     /name="IS66-like element ISEc23 family transposase"
     gene            complement(13028..13378)
                     /gene="tnpB"
                     /locus_tag="GZS11_RS23665"
                     /old_locus_tag="GZS11_23665"
     CDS             complement(13028..13378)
                     /gene="tnpB"
                     /locus_tag="GZS11_RS23665"
                     /old_locus_tag="GZS11_23665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="WP_000624722.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLFI
                     FRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDWRQ
                     PKTSSRNSLTML"
                     /name="tnpB"
     gene            complement(13375..13800)
                     /locus_tag="GZS11_RS23670"
                     /old_locus_tag="GZS11_23670"
     CDS             complement(13375..13800)
                     /locus_tag="GZS11_RS23670"
                     /old_locus_tag="GZS11_23670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_032346680.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMVELASQPGACVAQIARENGVNDN
                     VIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQTR
                     VSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
                     /name="transposase"
     gene            <13991..14170
                     /locus_tag="GZS11_RS23675"
                     /old_locus_tag="GZS11_23675"
                     /pseudo=""
     CDS             <13991..14170
                     /locus_tag="GZS11_RS23675"
                     /old_locus_tag="GZS11_23675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /name="IS66 family transposase"
     gene            complement(14531..>15007)
                     /locus_tag="GZS11_RS23685"
                     /old_locus_tag="GZS11_23685"
                     /pseudo=""
     CDS             complement(14531..>15007)
                     /locus_tag="GZS11_RS23685"
                     /old_locus_tag="GZS11_23685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /name="IS256 family transposase"
     gene            complement(15064..17745)
                     /locus_tag="GZS11_RS23690"
                     /old_locus_tag="GZS11_23690"
     CDS             complement(15064..17745)
                     /locus_tag="GZS11_RS23690"
                     /old_locus_tag="GZS11_23690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043988906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrlF family AAA-like ATPase"
                     /protein_id="WP_000678689.1"
                     /translation="MKGSEWNKWDLHIHSPMTWQANGYSSQCDIEHYVHLLREKQLSLI
                     AVTNYFYFRQNELETIREEIARQGLNITVLGNVEFRLDQQNKAGDFINVHILFSDKVST
                     ERINEALARVPLRLTDGDRKAIYCCEKSVTESGHGVDTIVVDFSALLGHLSSAFRPFKD
                     YLVAVCPNGYGGYRPDASGRSAAIATEIDRQGQIIFGGKGDREFFLRTNRYDGASIKPV
                     FLCSDAHRVEDIGSRYTWVKALPTFEGLRQALLEPEDRLRLGDEWLTELTPKTHFSQID
                     LEGTIFDGQEIRFKKLSIPLNQDMVAIIGGRGTGKSLLLDALRSRFAGTATRGSEQREV
                     NVQYLSIELDKANGEKMRFDAPSEGYEYLHVSQGEIKKLCQEPGLISDEIKKMLRLTPI
                     HEAGDTVAQLAENLNIWRAWREFSLYRNEHSEPVNTRKFQQQIIRGAQEKIEVLTSDRN
                     KALIETFRENSRRQTLLVQMRDKLTGVRADITTAENNLNASIAAINASVTTKEAIPLVD
                     LSAQTEAVACRLLSIQEQTTQFGRDNDEIIGTFREQGLGQDITGLLEKVHEYQRQMELA
                     ETHLHEIERRERQYQTGLEQRAKVAAEFIDGLLSRQSVIDTAFASLTTKPHLTQDQQTL
                     IKDILTDIRIYGKPHFDIKAFYEGMLSCLNRGRFRASGELTSVDRLRQVFRVGSIDDFR
                     ALLANKPMLVLPEFSNRKVTIEEFLWCDEYFNSPGPDSLLSYLFSPEQIQRYLNVRAEF
                     EYKGKTVEKLSAGQRGTFYVCLKLATDAFGSPFVFDQPEDDLDNDFIMHSLVPLFRKIK
                     QYRQVIIVTHNANLVVNCDAEQVIIATNNDEIISYRSGALEYGDHDAPNSMRKAICDVL
                     EGGHQAFEAREQKYGMLWLKTI"
                     /name="TrlF family AAA-like ATPase"
     gene            18280..18594
                     /locus_tag="GZS11_RS23695"
                     /old_locus_tag="GZS11_23695"
     CDS             18280..18594
                     /locus_tag="GZS11_RS23695"
                     /old_locus_tag="GZS11_23695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162676773.1"
                     /translation="MGKPIFRSFYDVVRELEDVYGHKELWLYSGAAYATPTEMINARHN
                     WKSPKILKRNGRMVAERMDNSDSRQLVGDYKKPLFQHYAPPWQSCQIDDYFKGYYIIAP
                     "
                     /name="hypothetical protein"
     gene            18647..19426
                     /locus_tag="GZS11_RS23700"
                     /old_locus_tag="GZS11_23700"
     CDS             18647..19426
                     /locus_tag="GZS11_RS23700"
                     /old_locus_tag="GZS11_23700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000535296.1"
                     /translation="MGSMKELLFEMQEERRDEWIAENYPDAEEGTPEWDAAAQEYSWFQ
                     DWMEEAAEQQYFEASLASIPDRLQDAKAELDELESLMQFNQPRIVERMAYVHCVSVLDS
                     FLMYSARALLSHPPHLQKFLHEADSLVPNKEDRRKLLASKWVEQEPDKDTPEKVYTWRA
                     QSLVAKKTFQSHKVIGRYFSRMLTTPHEWPLEEIKGVIKTRNALVHRNGVTESLEPVYI
                     SSGSVQNAICTVRAFITVAAETLLQEDALYRTDDGIF"
                     /name="hypothetical protein"
     gene            19471..20256
                     /locus_tag="GZS11_RS23705"
                     /old_locus_tag="GZS11_23705"
     CDS             19471..20256
                     /locus_tag="GZS11_RS23705"
                     /old_locus_tag="GZS11_23705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749202.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_077249540.1"
                     /translation="MSMTSLPANCIPVSNTSLPVAIDYPAALALRQMALVQDELPKYLL
                     APEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQRT
                     EKAARTAGRMPAGQQTHRLVPLSDAWFVSQLQTMVATLKIPMERRNKRTGRTEKARIWE
                     VTDRTVRTWIGQAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMGHKS
                     ISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKNRHA"
                     /name="site-specific integrase"
     gene            complement(20394..20669)
                     /locus_tag="GZS11_RS23710"
                     /old_locus_tag="GZS11_23710"
     CDS             complement(20394..20669)
                     /locus_tag="GZS11_RS23710"
                     /old_locus_tag="GZS11_23710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000239529.1"
                     /translation="MVKKPSQQALNRAAVTVEQAEALAQRLADKPYGAPEKPEPEKQCR
                     TTISLGESMLVTIEDLALRNKRNGKDPKNVSAIVRVALEQYLKTLT"
                     /name="hypothetical protein"
     gene            complement(20663..21307)
                     /locus_tag="GZS11_RS23715"
                     /old_locus_tag="GZS11_23715"
     CDS             complement(20663..21307)
                     /locus_tag="GZS11_RS23715"
                     /old_locus_tag="GZS11_23715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000633913.1"
                     /translation="MITVVGGNKGGSGKTTIASNLAIALANKGREVCLLNGDLQRTAAK
                     HHAEREAAGLLPAITLVEKFDNLTQTLQALNEKFDDVIVDVAGRNSKEFITSGVVAHQI
                     IAPLQCSQPDLDTLTELEQQIDAMRNLNPELKVYCLQSMATTNPVLRGNERKEFLEYLE
                     EFPTIQVLDSVICFRKVYRDCMSNGTGVVETNNTAARAEIEHLMNEVFGPW"
                     /name="division plane positioning ATPase MipZ"
     gene            21536..>21802
                     /gene="parM"
                     /locus_tag="GZS11_RS23720"
                     /old_locus_tag="GZS11_23720"
                     /pseudo=""
     CDS             21536..>21802
                     /gene="parM"
                     /locus_tag="GZS11_RS23720"
                     /old_locus_tag="GZS11_23720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505082.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /name="parM"
     gene            complement(21802..22050)
                     /locus_tag="GZS11_RS23725"
                     /old_locus_tag="GZS11_23725"
     CDS             complement(21802..22050)
                     /locus_tag="GZS11_RS23725"
                     /old_locus_tag="GZS11_23725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="WP_000618110.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRIDQ
                     GSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
                     /name="DinI-like family protein"
     gene            22468..>22695
                     /locus_tag="GZS11_RS23730"
                     /old_locus_tag="GZS11_23730"
                     /pseudo=""
     CDS             22468..>22695
                     /locus_tag="GZS11_RS23730"
                     /old_locus_tag="GZS11_23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /name="DUF1281 domain-containing protein"
     gene            <22697..22921
                     /locus_tag="GZS11_RS23735"
                     /old_locus_tag="GZS11_23735"
                     /pseudo=""
     CDS             <22697..22921
                     /locus_tag="GZS11_RS23735"
                     /old_locus_tag="GZS11_23735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /name="antirestriction protein"
     gene            22968..>23078
                     /locus_tag="GZS11_RS23740"
                     /old_locus_tag="GZS11_23740"
                     /pseudo=""
     CDS             22968..>23078
                     /locus_tag="GZS11_RS23740"
                     /old_locus_tag="GZS11_23740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            <23075..23338
                     /gene="finO"
                     /locus_tag="GZS11_RS23745"
                     /old_locus_tag="GZS11_23745"
                     /pseudo=""
     CDS             <23075..23338
                     /gene="finO"
                     /locus_tag="GZS11_RS23745"
                     /old_locus_tag="GZS11_23745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /name="finO"
     gene            23470..23682
                     /locus_tag="GZS11_RS23750"
                     /old_locus_tag="GZS11_23750"
     CDS             23470..23682
                     /locus_tag="GZS11_RS23750"
                     /old_locus_tag="GZS11_23750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001385659.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAASV
                     APRTDWQQFARKRAEHCHRRCRGRV"
                     /name="hypothetical protein"
     gene            23970..24647
                     /locus_tag="GZS11_RS23755"
                     /old_locus_tag="GZS11_23755"
     CDS             23970..24647
                     /locus_tag="GZS11_RS23755"
                     /old_locus_tag="GZS11_23755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="WP_089564147.1"
                     /translation="MEENNTSTDKNTLTALIDYENCGSLKNISLEQYGELIIFVGPQQN
                     VVVLPADSFPEGARITIRQVSGVSRNNVDFHLVLELGRISCCAAGKDKTYHIISSDKGY
                     DGVIRTLKEAGITCIRRDVPESIAAAGESGADKSNCLKQPSVPESDIVSRWAGKLQQIA
                     GSGAGNMPVSVETFNNYLKSHMRDEWNKTRAVAIRNELVHRGVTEVREKKIIWKKAKKE
                     NKA"
                     /name="PIN domain-containing protein"
     gene            25026..25217
                     /locus_tag="GZS11_RS23760"
                     /old_locus_tag="GZS11_23760"
     CDS             25026..25217
                     /locus_tag="GZS11_RS23760"
                     /old_locus_tag="GZS11_23760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071849536.1"
                     /translation="MNGFRNSSRNGQVWRYQSAGSRAVILEAAEAWRRAAGVAPRTDWQ
                     QFARKRAEHCHRRCRGRV"
                     /name="hypothetical protein"
     gene            25461..>25565
                     /locus_tag="GZS11_RS23970"
                     /pseudo=""
     CDS             25461..>25565
                     /locus_tag="GZS11_RS23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /name="endonuclease"
     gene            complement(25623..26591)
                     /locus_tag="GZS11_RS23765"
                     /old_locus_tag="GZS11_23765"
     CDS             complement(25623..26591)
                     /locus_tag="GZS11_RS23765"
                     /old_locus_tag="GZS11_23765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903B family transposase"
                     /protein_id="WP_162676775.1"
                     /translation="MKDQITHLPDNADRSVAKQKFKITNWPTYNKTLINRGSITFWLDD
                     EAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVPLR
                     CPDYSCVSRRAKSVNISFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRIWRK
                     LHLAVDSNTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRAASADGAYDTRLCHDELRR
                     KKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETAMYRVK
                     QLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
                     /name="IS5-like element IS903B family transposase"
     gene            <26626..26988
                     /locus_tag="GZS11_RS23770"
                     /old_locus_tag="GZS11_23770"
                     /pseudo=""
     CDS             <26626..26988
                     /locus_tag="GZS11_RS23770"
                     /old_locus_tag="GZS11_23770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /name="thermonuclease family protein"
     gene            27034..27243
                     /gene="hha"
                     /locus_tag="GZS11_RS23775"
                     /old_locus_tag="GZS11_23775"
     CDS             27034..27243
                     /gene="hha"
                     /locus_tag="GZS11_RS23775"
                     /old_locus_tag="GZS11_23775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="WP_001333231.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAAD
                     HRLAELITGKLYDRIPKEIWKYVR"
                     /name="hha"
     gene            27281..27871
                     /locus_tag="GZS11_RS23780"
                     /old_locus_tag="GZS11_23780"
     CDS             27281..27871
                     /locus_tag="GZS11_RS23780"
                     /old_locus_tag="GZS11_23780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="WP_012817838.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGDA
                     ERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNENIRPRS
                     RQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQAGI
                     PLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
                     /name="DUF2726 domain-containing protein"
     gene            28111..28371
                     /locus_tag="GZS11_RS23785"
                     /old_locus_tag="GZS11_23785"
     CDS             28111..28371
                     /locus_tag="GZS11_RS23785"
                     /old_locus_tag="GZS11_23785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="WP_000083819.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEIK
                     VFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
                     /name="replication regulatory protein RepA"
     gene            complement(28397..28633)
                     /locus_tag="GZS11_RS24325"
     CDS             complement(28397..28633)
                     /locus_tag="GZS11_RS24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001410080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="WP_244569896.1"
                     /translation="MSTERSLELFRAYNYTPRIAELLAIRFLDGPVNLLLVAKLAEDYR
                     GFHFSGSCTSTSEPVPCHLMAWPAIKFLKRSVI"
                     /name="replication protein RepA"
     Typing          2..858
                     /name="IncFIA"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.576"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(28406..28663)
                     /name="IncFIC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.0"
                     /coverage="99.0"
                     /evalue="3.93e-111"
ORIGIN
        1 gtgactgatc ttcaacaaac gtattaccgc caggtaaaga acccgaatcc ggtgtttaca
       61 ccccgtaaag gtgccggaac gctgaagttc tgcgaaaaac tgatggaaaa ggcggtgggc
      121 ttcacctccc gttttgattt cgctattcat gtggcgcatg cccgttcgaa gggactgcgt
      181 cggcgcatgc caccggtgct gcgtcgacgg gctattgatg cgctgctgca ggggctgtgt
      241 ttccactatg acccgctggc caaccgtgtc cagtgctcca tcaccacgct ggccattgag
      301 tgcgggctgg cgacggagtc tgctgccgga aaactctcca tcacccgtgc cgcccgggcc
      361 ctgacgttcc tgtcagagct gggactgatt acctaccaga cggaatatga cccgcttatc
      421 gggtgctaca ttccgaccga catcacgttc acatctgcac tgtttgctgc cctcgatgta
      481 tcagaggagg cagtggccgc cgcgcgccgc agtcgtgttg aatgggaaaa cagacagcgc
      541 aaaaagcagg ggctggatac cctgggtatg gatgaactga tagcgaaagc ctggcgtttt
      601 gtccgtgagc gtttccgcag ttaccagaca gagcttaagt cccggggaat aaagcgtgcc
      661 cgtgcgcgtc gtgatgcggg cagggaacgt caggatatcg tcaccctggt gaaacggcag
      721 ctgacgcgtg aaatcgcgga agggcgcttc actgccagtc gtgaggcggt aaaacgtgaa
      781 gtggagcgtc gtgtgaagga gcgcatgatt ctgtcacgta accgcaatta cagccggctg
      841 gccaccgctt ccccctgaaa gtgacctcct cagaataatc cggcctgcgc cggaggcatc
      901 cgcacgtctg aagcccgcca gtgcacaaaa aaacagcgcc acatacaaaa aacaacctca
      961 tcatccacct tcaggtgcat ccggtccccc ctgttttcga tacaaaatac gcctcactga
     1021 cggggaattt tgcttatcca cattaaactg caagggactt cccgataagg ttacaaccgt
     1081 tcatctcata aagcgccagc cgtcagcgtt acagggtgca atgtatcttt taaacacctg
     1141 tttatatctc ctttaaacta cttaattaca ttcatttaaa aagaaaacct attcactgcc
     1201 tgtcctgtgg acagacagat atgcacctcc caccgcaagc ggcgggcccc gaccggagcc
     1261 gctttagtta caacacacaa agacaacctc cagaaaaacc ccggtcccgc gcagaactga
     1321 aaccacaaag cccctccctc ataactgaaa agcgaggccg cccccgcccg aagggcggga
     1381 acaacatcgc ttttaattat gaatgttgta actaagaagc atcattgctg tcagtcttct
     1441 cgctggaagt attgagaaca cgctcgtaag cggccctgac agcccgctaa cgcggagata
     1501 cgccccgact tcgggtaaac cctcgtcggg gccactccga ccgcgcacag aagctctgtc
     1561 atggctgaaa gcgggtatcg tctggcaggg ctggggatgg gtaaggtgaa atctgtcaat
     1621 cagtaccggc ttacgccggg cttcggcggt tttattcctg tgtcatatgt aacaacggaa
     1681 tgccgccttc catgccgctg gcgcggcata tcctggtgac gatatctgaa tcgttatata
     1741 ctgtgtatat acgtagtaat gacgaggtga taaatggcac aggttaatat gagtttaaga
     1801 atcgacgctg aactgaagga tgcttttatg gccgctgcaa aaagcatgga ccgtaatggc
     1861 tctcagttaa tccgggattt tatgcgccag accgttgaac ggcagcataa tacctggttc
     1921 cgtgaccagg ttgcggcagg acgtcagcaa ctcgagcgcg gcgatgtgct tccccatgac
     1981 atggtcgaat cttctgccgc tgcatggcgg gatgaaatga gcaggaaggt tgccgataaa
     2041 tgatggagat attctggacc atgctggcca gccaggacag gaagcgcatt cgtgagtaca
     2101 ttgcggagca gaacctgatg gccgccattg aactggacga acggattggt tattcagcca
     2161 gtagcctggc cgggcagccg tataaaggtc gcaacggtcg tgtggaaggt acccgggaac
     2221 tggttattca cccgcatttt gttctggttt atgaggtcga tagccagtgg ggaaaagtgt
     2281 atatcctgcg tgtattgcat actgcacaga agtggccata aagaagagta tatttttacc
     2341 ttactggggg ctgattatct gttgttactg ttgtggcata catagaaaaa cagttgggta
     2401 gttaaaatag cataaaggtt tgtgaaatga ttggaaaatc tatatctcgt ttatgccaaa
     2461 atgaacgcaa aaacagcatt gcacaatctt gatcgatagg aatttaaatc cccaaaagac
     2521 taaaaaagca ccacaaaacg gatatttctt caacactcct tatgcaccac atgaacggaa
     2581 acgactatta aacaggataa cactgattga taaagggtat gaatggtggt tttttgctcc
     2641 gttccccgta aaatggacgc aacttcccct ctgcggctct cagccgcaac actgcattcg
     2701 ggccagcggc tcatgcatca taaccagctc tgccagacgg tatccccgct tcagccccgt
     2761 aaaccgcatc tgacttccgc acaggacgca tttcagcggg tcaaccttca gtaaccgctg
     2821 atacatccct ctccacctga tttgcatcgc cgtttttctc accgtctccg ttatgacgta
     2881 caccacctct tccagtaagc ggcgcttcac cggactcagg aaaccgtagt acctcaccat
     2941 acggaacccc ttatccgcca catgccacga gaacctttcc atgaactcat cgccactcat
     3001 caccaggtat tcttcccgtt ttgtacggtg actgttgtaa cgcagaccga tttcatcctg
     3061 accggcataa tgctccagcc gactcatcgg caccggtggc tttttcaggt aagaaccaaa
     3121 gtacaccgcc acatgcgtgg cgttatccat cacccgggat acgttgacat tccagccacg
     3181 ccggtagtgg gtgtccagga agcgattcca ttcccgtctg ccgcttccct ctgctgccag
     3241 ctcagccggt atcaccagtt ccgggtattt ccgggacagt aacatggtgt atacggagaa
     3301 gcacatccgt atcatcagtg tgacgactgc ggcggccatc catccagtca tcggttcgtc
     3361 tgagaatgac gtgcaactgc gcacgcgaca cccggagaca acggctgact aagcttactc
     3421 cccatccccg ggcaataagg gcgcgtgcgc tatccacttt tttgcccgtc catattcaac
     3481 ggcttctttg aggagttcat tttccatcgt tttcttgccg agcaggcgct ggagttcttt
     3541 aatctgcttc atggcggcag caagttcaga ggcaggaaca acctgttctc cggcggcgac
     3601 agcagtaaga cttccttcct ggtattgctt acgccagaga aataactggc tggctgctac
     3661 accatgttgc cgggcaacga gggagaccgt catccccggt tcaaagctct gctgaacaat
     3721 tgcgatcttt tcctgtgtgg tacgccgtct gcgtttctcc ggccctaaga catcaatcat
     3781 ctgttctcca atgactagtc taaaaactag tattaagact atcacttatt taagtgatat
     3841 tggttgtctg gagattcagg ggggcagtct agcagcaaag aaccttgcag aggcgttcga
     3901 gcattataac gaatggcatc cgcatagtgc actgggttat cgctcgccac gggaatatct
     3961 gcgaacgctt ctggttcagg gggctcgttc ggttttgatt ggtgcagaga aaaggactga
     4021 ctcattcagc cgttgggttt gtacgttggt tgagcgcagg gggtactggc gggctgttgt
     4081 agccatcgcc gccaaaaacg cgaggctgtg ttgggcatcg ttgcattacg gtgatgattt
     4141 caggctgtac tcagccagct aaagcacaaa gcagtataac catctgacct gcaactcgtt
     4201 gatgaaaaag ggttagaccc cgtgaggtct atctggctat tgtacaggat atacgtatcc
     4261 gcttaacgaa caagaacttc acgcgcgtct ttcatcaggg cccgaatcga taacgattca
     4321 tcatggccgt ttatagcacc gcagtctctc ccctttattt tactgacgag cagacagaaa
     4381 ctataaaacc ggcgttgaca tgccggggaa gcccttatag tacaataatt ttcgatatcc
     4441 aaactgaccc cgaaaagaat gctgaaaggc tgccagtttt ttctttagtt gctctgaaaa
     4501 tccatgtatt atatgtaaca taacatataa tacatgggtg ttaatatggc aaaaggtgaa
     4561 tcaggacgca tcgtcctaga agttgagcca gaactaaaaa aagctctcta ttcagttctt
     4621 gcaatggaac aaaaaactct taaagactgg tttgttgata aggctcaaga acatatatgc
     4681 gagaaaaaat cagagctcat agagagattt tcgaaggtag acaatgaaat ttaaagcaga
     4741 tcaaacctca caaaaactta gaggtggcta ttacacccca caaaatttag ctgattatgt
     4801 aactaaatgg gtcttaagta agaatcctaa aaccatactt gagccgagtt gtggtgatgg
     4861 tgtgtttatt caggctattg ctaataatgg ttataactct aacattgagt tattttgctt
     4921 tgaattgttc gacactgaag catctaaggc tctcgaacgc tgtaaactga acaatttttc
     4981 taatgcaacg attactgagg gggattttct agtctgggct aatgaatgtc ttaagaaaaa
     5041 taagcagata tttgatggag ctttaggtaa tcctccattc atccgttatc agttccttga
     5101 aagaaatttc caagaacaag ctcagttggt cttcgaacat ttagatctaa aatttacaaa
     5161 gcacactaat gcttgggttc cattcttact ttcttcatta gcccttctta aacaaggtgg
     5221 aagaataggg atggtcatac catcggagat tagtcatgta atgcacgctc aatcattgag
     5281 aagctactta ggtcatgttt gctctaagat tgtcatcatt gaccctaaag agatttggtt
     5341 tgaagataca ttacaggggg ccgttattct ccttgcagag aaaaagcaat atccagatga
     5401 agcatcgcaa ggtgtaggta ttgttagtgt tagtggtttt gaattccttc aagaagatcc
     5461 aaacgttctg tttaatgata cggcaggaat aaatggtgaa acagttgaag gtaaatggac
     5521 taaagcaaca ctcagtattg acgagcttca gctaattaaa cgggtaattg ctcatccaga
     5581 tgttcgtaaa tttaaagata tagctaaagt tgatgtaggt attgtgacgg gggccaacaa
     5641 ttattttctt gttgataatg aaacagttaa attatataaa ttagaacgtt ttgctcaccc
     5701 tatgtttggc agaagccaac attgccccgg aattatttat gatgagaagc agcacgttga
     5761 aaaccagaaa aagggtttac caaccaattt tttatatata gatgaagagt ttgaatatct
     5821 ttctaaaagt gtcaaaaatt acataaagtt aggtgaagta gaggaatacc acaagcgtta
     5881 taagtgccgt atacgtaagc cttggttcaa ggtcccatct gtatatagca cagagattgg
     5941 aatgttaaag cgttgtcacg atgcgccacg cttaattcat aacagagtta gagcatatac
     6001 cactgatact gcatatagag tttcttcaac agttactagt actgagaact tagtgtgtag
     6061 ttttttgaac cctataacgg tcattacagc cgaactcgaa ggacgctttt acggcggtgg
     6121 cgttcttgaa cttgtcccat ccgagataga aaaactatat atccctatcg ttgaaggatt
     6181 agaacataac gttgaagaac tcaacctatt gattaaagat ggtcaaatag agagggtaat
     6241 tcgacaacaa ggttcactaa tcttagacaa gcttggtttc actcaagaag aaaatgaaaa
     6301 acttgtagaa atatggaaga agcttagaga tagaaggcta cgtaagtagg gtttaaaaag
     6361 tagggggctc ataaaaaatc aatgcagctt ttagctgcat tgattttatc tggtaacctt
     6421 ataggttttt gtatagatct tctactgttg gaatctcgga atctaaatcc cccaagtcaa
     6481 aaagattctt aatcaaataa tccatttgga ttttttcttg ttcaaattgc ctttcgaata
     6541 tgattttatc tctatcagct gatttttgag cctggctata cagtttattc aaatactgtt
     6601 gctttgaact gatcgtatca tgtatttctt tctgttttgg atcatcaaaa tcaatagtag
     6661 gaataggcat tctagtttga acttttgtcc cacgagcaac aaatctacct ctaaaaatat
     6721 caccatatat tgaagcaaac cactcaaggt actttgatgt aaggattgct tggatataat
     6781 agatagagta tctaacgtta ttaggaacgt tgataatgct atagcccgca gtgcctccag
     6841 aagaaacaaa agttctatgg ttatcaatgc tgtacttata tccattagat agaataccaa
     6901 caatcagctt ttgatcaaca tcgcagtttt ctaatgcttg gctacgtccg tatctgtacc
     6961 attcattagg cccagttggg tcaggcttaa tagagcgttt cttatcattc aaatggactt
     7021 ttacaacctg caggaactca aataacttag gatattgacg ttttagttca tcgtattcaa
     7081 tgaactggat tctttcaccc actttcttgt atgggtaaac aacaaatgaa ttaggttcaa
     7141 cgtctttata agtatagaaa ctatcatcac ctgaacgatt cgtttcgaag taagggcgag
     7201 ttaattcttt ttcaatgtgg tattcaattc catcatattc aaaataaata aaaccatttt
     7261 cagatttgat ttctttatga atgtagtatt tatttgcact tgtctgaatg ccatttgcaa
     7321 cattactttt tcctacaatg ttacctagct gttcagattt tgaaaacatt agcttcaaaa
     7381 tgtcattgat tttcttttct agaatccaag tgtcagaatc aagagaactg gtttgatatg
     7441 tgcttgataa taaatattta tcttcacgag ttaaccactt cttgaagtct tttacttcat
     7501 aaaaagaaaa gttatcgtga ttttctttat ttaaaaataa caaacaagtg taagttgttt
     7561 tattcttgaa tacttgataa gaaccaaaag aaataagttt agataaatat ttattttctg
     7621 ataaaaactt gcgtagtttt ttacctgcat ctacttttat gaacctagac ggaagaatat
     7681 agcctaaata gccgtgatct ttaagaattt gtattgaacg ctcaataaat aaaaagtatt
     7741 tatcgaattg cttataagca gatttatatt tcctcttgta aatatctaat tcctttggtg
     7801 ttagctgatt catatgctca gtcgccatat aaggaggatt tccaacgatc acatcaaatt
     7861 ggtagttagt caaatcaaat ggattaattg aaaaaatatc ttcttgttta actttgtctc
     7921 cactatctat caagctattc ccgaatagaa tgttcgtatc cagcgcaggc aaaatagggg
     7981 tgtctttacc aatggtttct gtagtttcgc cttcgagaag ctttagcaat aaaccaaagg
     8041 tacaagcttt tgttgcatta tagtctttat caatcccata tatacattta catagaattt
     8101 ctctttttac ttcaaattta agtttgtatg tatgttcaga tatttgctga agtttagatt
     8161 tgtcattttg tatataataa tcaataagaa tatcctggat gaattggaat gcctcaataa
     8221 taaaagcacc tgagccacaa gcgatatcag caaattttga attcaatatc tcaatatctg
     8281 acttgccttt gcaatattct acgacagtat ttctaatgat ttcttttaca atgtgagttg
     8341 gtgtagtaac aacgtctcta tcgatatgtt cctctttcgg ttgaatcgta acgtttccaa
     8401 gttcatcaat acgaactttc tcactcagga atatttcata tatattacct aaaatatcag
     8461 aagagaaaac agagaaagag tatgtgcttt gtgggaagta taattgttct atgatgctcc
     8521 aaatgcaaga gcaagcattg cttaataact catcaatata ttctagcgag aataatcctg
     8581 aattatattt cttgtctgag gatttcaatt ttttgactaa agattgaaaa tccttatctt
     8641 gtgcaaagtg ataaagagtt tcgtattctt ccaagtctct atcttcacag actctaagga
     8701 aaacaatact gttaatataa ttttgtacaa gatcatttaa tttttcttct ggaagttcgt
     8761 tttttatctg caaaaattca ttagcaagaa gtaagcgcca atcattaatt tgcttgagga
     8821 atagatcatc aacactgaat ctcagtatct tattttcaat ttctgaccat tcattgtcaa
     8881 aatgaccagt gtaaacactt tctcgaccga taaggttatt aatttcatca aacttatcta
     8941 cgagttcagt gaagtgatat aacttgattc tagagtttgc tactgaatca gtttctttaa
     9001 cctgattaga gcaatcataa attgccgtat actcgaaatt cgacaacaca gaaattttta
     9061 atttggctgt gaatccgtat cgtctaactt gtagagctgg ttctatagta gttgatatat
     9121 caatactagg ctttttggct tcaaggaaaa atttacgctc agagaagagt ctaaacgtgt
     9181 agtctggctt tttagtattt tcgcctgctc gggcttttaa accttcctct acaagcactt
     9241 cgcgttcgtt tgttggtttt cctgctgcgt tcgttatgtc ccaaccgaga ataaggaata
     9301 gttgatctaa aaaatccgtt cttagttgag tttcgttgta tctggcagaa cgataataat
     9361 caatatcgcc tttgtattta tcaattagtc tttgtaactg atctttttta ttcatctatt
     9421 tctcacaacg atatttatca aaaagcagat ttctaatgat tgtaattctt gtgccgaagt
     9481 attgttagtc aagttatcct aacagttcca ttctttacgt ctcttgttgt gctgctcaga
     9541 ttttgctgtc actattttta cctcaaaaac cacagtaaca ctaccaatag gtggcttttc
     9601 gatgatgact gcaaggaaag tgtggtatag caagtattcc aatgagataa agggcttccg
     9661 gatcgcgcac accacgacga tcgcggacga aatcactgaa cgcccatatc agctctttaa
     9721 gtatgtcttc acagtccgat gaacgacgat ccagcttcgg gtgtcatcac cttgtccggt
     9781 gacaaaccgc accaactggt cgcttaggta cttgtagaag ataaaaccga gtatgtaatc
     9841 tttgtattcg ttagcttcaa ttttagaccg catctggttg gcagattccc agatttttgc
     9901 agcaatctgt tgtttattgt caacgacgga tgaaagtggc tcttccgcag cttgggtgga
     9961 gattttcatg ccagtggact cattaccctc tgcctgagta gttcaaatcc tgctcgacca
    10021 tacatctggc gtttcagcat cttcaggcga tttacatgac cttcgacaac accattactc
    10081 cagcgactgc tgattgcctc acatatagcc gcaacatcag cttccatccc cgtagccacg
    10141 cgccgaagtt ctgctgaacc gctttcgtgg actcgagtga accaactgct tagctgtgat
    10201 ttattttggg ttttcagaat acggtagaac tcgagtgcca gttgctgcgc tattttcagc
    10261 tcaggttctt tttcacacat cagactaata aatcgggaag cataattttc ttcatccctg
    10321 gttattctcc cgggcatcaa ccagcggctt acccggaaca ctgaaggaag tctgacggca
    10381 gtagtaaccg gtgggttcca gcctttacgc catttggcaa ccgcatccct gacgatggtt
    10441 tcactgcctg taaatccccg ggccaccatt tcccgccata tccggctggc gttataatta
    10501 ccactttctc gttgaaccgc cccgggtttc ctggagagtg ttttatctgt gaactcaggc
    10561 tgccagatca tcgtttccga tggaagcata ataagctttt tctgcttctg ccggaggagt
    10621 atggcccagc ctttccagca atcgtcgatt gttataccag tccacccacg ttagtgtggc
    10681 cagttccact tctgcacggt ttttccagct cttacggtgt attacctccg ctttgtaaag
    10741 accattgatg ctctcagcca tcgcgttgtc atacgagtcg cctgtacttc ctgttgatgc
    10801 cagtaatccg gcttccttaa gccgctgtgt gtaggccagc gatacatact gagaaccttt
    10861 atcactgtga tggaccgtgc cggacggtcg acgggcccat aacgcctgct ccagtgcatc
    10921 cagcacgaat gtcgtttcca tggacgatga gacccgccac cccacgatgt atccggcaaa
    10981 cacatcaatg atgaacgcca catagacgaa gccccgccat gtgctgacgt aagtaaaatc
    11041 agccacccac agctggtcag gtcgttctgc cacgaactga cggtttacgc ggtcgcctgc
    11101 ggcaacggct ttccggctga tggtcgtacg gaccttttta ccccggagaa caccggcaag
    11161 tcccataacc gccatgagac gtgccactgt acatctggcc accctgattc cttcccgtaa
    11221 caactgacgc cagactttac gcacaccgta cacctgatga ttttcatcgt atacgcgctg
    11281 tatctctctc ttcagccagt cgtcgtgctg cgcacgggca ctgcgtttat ccggatgatg
    11341 tcgctgttgc tgagtaagcg tcaacggagc accgtattga cgcttattta ttggtgagta
    11401 ctacgttcca tggcaggagt tcatcaactc ggttggaagg ccattccggc agtacgctca
    11461 ggatatggcg cagatacgct tccggatcga taccgttcag acggcaggtg ccgatcagcc
    11521 cgtacaacag tgctccacgc tcgccgccgt gatcgctgcc aaagaacata aagtttttct
    11581 ttccgagaca gactgcacga agcgctcttt ccgcagcatt attatccgcc tccgccagac
    11641 cgtcatcact gtaataacag agggcatccc actgattcag tacatagctg aacgcttcgc
    11701 ccagtctgga ttttttcgac agcgtaccat tcttctccac catccattca tgcagcgacg
    11761 tcagtaacac tttgcttcgc tgctgcctga cggcaagacg ctctgactcc ggtaatcccc
    11821 gtatttcatc ctcgatggcg tacagttcac tgattcgctt cagggcttct tctgccgtcg
    11881 cacttttgct gctgatgtat acatcgtgga tttttcgccg ggcatgggcc cagcacgcaa
    11941 cttctgtcag tgcaccacct tcacgttctg cactgaacaa cctgtcgtaa cctgtgaacg
    12001 catccgcctg caggataccc cggaaggggc ggaggtgttg ctccgggtgt ttcccctgcc
    12061 ggttcggcga gtacgcgaac cagaccgctg gaggagatga cgaacccaca ttgcgatcat
    12121 cccggacata cgtcgagata cgccctgttt tcgccttttt ctgacccggt gccagtacct
    12181 ttaccggtgt gtcatcagtg tgaaccttgc gggtgttcat tacataacgg tacagggcat
    12241 cattcaccgg tgtcattaac tggcagcacg cgtcaaccca gttggagagt aaggcccggc
    12301 tcagttcgac accctggcgg gcaaagattt cactctgacg atacagtggc agatgttcgc
    12361 agtattttcc cgttaacacg cgggcaagta atccggggcc cgcgatacca cgctctatcg
    12421 ggcgggacgg cgccggtgct tcaacaatac agtcacattt tgtacaggct ttttttaccc
    12481 gttctgtgcg gatcactttc agggcactgc tcaccagttc cagctgttca gcgctgactt
    12541 cccccagata atccagctca ccgccacact ccgggcaaca gctttcttct ggctccaggc
    12601 ggtgtatttc acggggaagg tgtgccggta acggacgacg atggcgcgac tgtcgcaact
    12661 ggcggggaac ctgaggatcg tcttcccgcc cactgtaacg atcgctgtcc tgttcacgtt
    12721 gtttcagcag agcctcagcc agatcaactt cacgacgcag tttttcagag cgggtaccga
    12781 acagcatccg gcgcagtttt tctatctgag cccgcagatg ttctatttcc cgttcatctt
    12841 cttcgatctt ttcttcggca cgtgtcagtg cagagcgcag gaaggcttcc gtctcttcaa
    12901 ccagactcag ttgctggtct ttctgacgga gggcttcagc ctgctcagag agcaaccttt
    12961 ccagctctgc gatgcgaatg aggtatttct gactcatgac cgtttttata atgcggtcag
    13021 gagtttttta caacattgtc agtgagttac ggctggatgt ttttggctga cgccagtcca
    13081 gcttatcgag gagcattgcc agttgcgagc gggtaatgga taccttgccg tcacgtaccg
    13141 caggccagat aaactggcct tcctccaggc gtttggtgaa caggcacaga ccatcagcat
    13201 cagcccaaag aattttgacg gtgtcacccc gtcggccacg gaagataaac aggtgaccgg
    13261 agaagggatt atcattcagc acatgttgta cctgttctcc cagtccgttg aaggatttac
    13321 gcatatcggt aacgccggca acgagccaga tacgggtacc tgatgggagt gagatcatct
    13381 tcccctcccg gtaagttcac ggatcaacac cgtgagcagc tctggcgatg gattttccag
    13441 cgtcatgtta ccgtgacgga attccacctt gcaggaactg gcactgactc tggtctgagt
    13501 ggaagtggat aaagacggcg caatggccgc cacaggttct ttctgctcat ccggcgttat
    13561 ttctacaggt aataattcaa cgccagtgtc agaagaggtc gttaccggaa gacgccgcga
    13621 aacacgccct tcgttctgcc agagcctgag ccatttgaaa ataacattat cattgacgcc
    13681 attttcacgt gcaatctgtg caacacaagc tccaggttgt gatgccagtt ccaccatacg
    13741 aagtttgaat tcattcgaat actttttacg aggttctttt cgccagtcct gtaattccat
    13801 acttagatgt ccgtctatat cagatgggcg tctaagttac caattctcgt ctgatggcta
    13861 catacggcgg tcagtttacg cttactacta cgtgcatcga atgagtgatc aagagccgta
    13921 tgacgcgaga gtgtcacgta cggttccgtg agcagccggg gagtgaaatt ctctccggct
    13981 gactcgactc gcgggttccg acagcggtgg tgaacatgcg gcggtgttgt actcgctgat
    14041 cggcacatgc cgtctgaaca atgtggagcc agaaaagtgg ctgcgctacg tcattgaaca
    14101 tatccaggac tggccggcaa accgggtacg cgatctgttg ccctggaaag ttgatctgag
    14161 ctctcagtaa atatcaatac ggttctgatg agccgcttac catctatgca agggaatgta
    14221 cggaagtaag ccaaacgatt atcgaacggt cagaatattt cgtacagcct ttacgttcag
    14281 tgagttagct ggcctagatg taaccatccg ttcaactcaa ttctcttagc gtgggaaaat
    14341 ttccgaattc caagctgacc cctatctgaa agaaggcggt caaaactatg atgtgatggg
    14401 ctggaatcag gaacaactga ttaatgacat actcgaccag tatgaaaaac acctgcactt
    14461 cctgcacctc gttcgttaat cgagtaggag gcgggatgag cctgtttacc attttgtgta
    14521 aatgcctttc tcagaagtga ccgtcaaggc ggtcaccgaa ttcgataata aagcgactca
    14581 tggctatccg ccagtcctga agtggcatcg tccatttctg tgaggctgac tggatagcca
    14641 gcctcaccac ttttttcact gattcatctg tcgggaacac tttccgtttc tttatcgcat
    14701 gtctgatgat gctattcagc gattcgattg cgttagtggt gtagataacc ttgtggatcg
    14761 cctccgggta cacgaagaag gtggcaacat tcggccagtt tgcccgccag ctgcgactta
    14821 tctgcgggta ccttgcatcc cattccttcc cgaacgcatc cagcgcctgc aacgcgcctt
    14881 cttccgtggc tgcgcggtaa acctgtttca ggtcacgggt taccgcttta tagtctttcc
    14941 acgccacgaa ccgcaggctg ttgcgtacca tgtgtactat gcacaactgg atctgcgtct
    15001 ccgggtagaa acggtctgct tttttgaatt ggcagaaata tgtcctttac tctcagatgc
    15061 taattagatg gttttaagcc acaacattcc atatttttgt tcacgagctt cgaatgcctg
    15121 atggccccct tcaagtacat cgcatattgc cttgcgcatg ctgtttggcg catcatgatc
    15181 tccgtattcg agtgctccgc tacggtaact aataatctca tcattattag tggcgatgat
    15241 gacctgctca gcatcacagt tcaccacaag gttagcgtta tgcgttacga taataacttg
    15301 cctgtactgt tttatttttc ggaagagagg taccagactg tgcatgatga agtcattgtc
    15361 tagatcatct tctggctgat caaatacaaa cggtgatccg aaggcgtccg tcgccagctt
    15421 aaggcagaca tagaaggttc ctcgttggcc ggctgacaat ttttcgaccg ttttgccttt
    15481 gtattcaaat tccgccctga cgttcagata gcgctggatc tgctcagggc taaacagata
    15541 actgagtagt gaatccggtc cgggactgtt aaagtattca tcacaccaga ggaactcttc
    15601 gatggtcact tttctgttcg aaaactctgg cagaaccagc atgggtttat ttgctagcag
    15661 tgcccgaaag tcatcaatac tgccgactct gaacacctgt cgaagcctgt caacagaggt
    15721 cagctctccg cttgccctga atcgtcctcg gttcagacaa ctgagcatcc cttcataaaa
    15781 ggcctttata tcaaagtgtg gctttccata aatgcgaata tccgtaagaa tgtcctttat
    15841 cagggtctgt tggtcctgcg tcagatgtgg ttttgtggta agagatgcga aagccgtatc
    15901 aatcacactc tgtctggaca gcaatccatc tatgaattca gcagcaacct tggcgcgttg
    15961 ttctagccca gtctggtact gtctctctcg acgctctatt tcatgcagat gcgtttctgc
    16021 aagctccatt tgacgctgat attcatgtac tttttccagt aaccctgtga tgtcttgtcc
    16081 tagtccctgc tcgcggaaag tgccaataat ttcgtcgtta tcacgcccga actgtgtcgt
    16141 ttgctcctga atggatagaa gcctacacgc aacagcctct gtctgtgctg acagatctac
    16201 gagcgggatt gcttctttag ttgtaacgct agcattaatt gcagctatgc tggcatttag
    16261 gttattttcg gctgtcgtaa tgtcagcacg tacacccgtt aatttatccc gcatctgaac
    16321 cagcaatgtc tgacgccgac tgttttcacg aaatgtctca atcaatgctt tattgcggtc
    16381 acttgtgaga acctctattt tttcctgtgc tccccggatg atctgttgct gaaacttgcg
    16441 tgtattcacc ggctctgaat gttcattcct gtacaaactg aattcacgcc aggcacgcca
    16501 gatgttgagg ttttctgcca gttgtgcaac tgtatcaccc gcctcatgaa ttggtgtcag
    16561 tctcagcatt ttcttaattt catcactgat gagccccggt tcctggcaga gctttttgat
    16621 ttcaccctga gacacatgca gatattcata cccttctgaa ggggcatcaa atcgcatctt
    16681 ctcaccattg gctttatcta attcaatgct tagatactga acattcactt cacgttgttc
    16741 gcttccccgt gttgcggtac cggcaaagcg ggaacggaga gcatcaagca gcaggctttt
    16801 acccgtgcct cggccaccaa taattgcaac catatcctgg ttaaggggga tggacagctt
    16861 cttgaatctg atttcttggc catcaaaaat cgtcccttca agatcgatct gactgaaatg
    16921 tgtttttggc gtgagttcag tcagccactc atctccgagc ctaagacggt cttcaggttc
    16981 cagtaacgct tgtcgaagcc cctcgaaggt aggaagtgcc tttacccaag tataacggct
    17041 accgatatcc tccactcgat gcgcatcgga gcagaggaag acgggtttga tggatgcacc
    17101 gtcataacgg tttgttctca gaaaaaattc acgatcgccc tttccgccga aaataatttg
    17161 tccctgacgg tcgatctcag ttgcgattgc cgcagagcga ccacttgcgt cgggccgata
    17221 gccgccatac ccgttcggac agacggccac taggtaatcc ttgaacggtc ggaatgcgga
    17281 actgagatgc ccaagcagtg cactgaagtc taccacaatg gtatccacac catgtccgct
    17341 ttccgtgacg ctcttttcac agcagtaaat ggcctttctg tcgccgtctg ttaaccttag
    17401 tggaacacgg gctagggctt cgttaatacg ttcggtgctc accttatctg aaaaaaggat
    17461 atggacatta ataaaatccc cggccttgtt ctgttgatca aggcggaatt ccacatttcc
    17521 cagcacggtg atattaagac cctgacgggc aatttcttcc ctgattgttt ccagttcatt
    17581 ctgccggaaa taaaaatagt tggtcacggc gataagggaa agttgcttct ctctaagtag
    17641 atgtacatag tgctctatat cacactgact ggagtaacca ttggcctgcc acgtcatcgg
    17701 gctgtggata tgcaaatccc atttattcca ttctgaacct ttcactgacg ttccttcata
    17761 ataagatgtt ttccttataa taatgaggaa atagttcttt tgctatttga tttcgccctg
    17821 ctttcctgaa tgctgctagc agcaaaggtt atagcttgca atgtaataat ccttattttc
    17881 tctctctgag atccgatttc tgtgagtcac ggatctgctt cagccccgcc ggcgcgcagc
    17941 ggcccggcaa cacaagatgt agcggtgttc ccagctgacg atgacgctag atgttgtgtc
    18001 cctcccctgc cccgtgaccc ggcgcgcagc agcagaaaac agtgcttcgc ttacttcctg
    18061 acagtggtac acttccggaa aatgcggaat aacataaaag tcggatctgg ccggaatggc
    18121 catccggtaa tttaattgag tggttatgtg gctgcttatt atcagcctgg aaatgatgac
    18181 gtcatgggaa aatcatccgt gtgacagaag cagggttgtg ctgcgcgtca tcattactct
    18241 gcgtgactat tgaaccggat actatttttg aggcgaggta tgggtaaacc aatatttaga
    18301 tcattttacg atgtggtccg ggagcttgag gatgtatatg gccataaaga gctatggctg
    18361 tattcaggag cggcatacgc aacgccaaca gaaatgatta acgccagaca taactggaaa
    18421 tcgccaaaaa tcctgaaacg taacggaaga atggttgcag aacgaatgga taactcggac
    18481 tcaaggcaac tggttggtga ctacaaaaaa ccgctgttcc aacattatgc tccgccctgg
    18541 caatcctgtc agatagatga ctattttaaa ggctattaca ttatcgcccc ctgaaggtca
    18601 gaggagtaat tgatggcggg attacatcat tgatggcgag gtttaaatgg gaagtatgaa
    18661 agaactgctg tttgaaatgc aggaggaacg gcgtgacgaa tggattgcgg aaaattatcc
    18721 ggatgctgag gaaggaactc ctgaatggga tgcggcagcg caggaataca gctggtttca
    18781 ggactggatg gaagaggcgg ctgagcagca atattttgag gcttcgcttg ccagtattcc
    18841 tgaccgcctg caggatgcaa aggctgaact ggatgagtta gagagcctga tgcagttcaa
    18901 ccaacccagg atcgtagagc ggatggctta cgttcattgc gtgagtgtac tggacagctt
    18961 tctgatgtac agcgcaagag cacttcttag tcatcccccg catttacaga agttcctgca
    19021 cgaggctgat tcgttggttc ctaataagga agacagaaga aaattacttg cttccaaatg
    19081 ggtcgagcag gagccggata aagacactcc tgagaaggtt tatacctggc gagctcagtc
    19141 tcttgtagcg aagaagacat ttcagagcca caaagtgata ggtcggtatt tttcaaggat
    19201 gctgacaacg ccacatgagt ggccattgga ggagattaag ggtgtaatca agacccgaaa
    19261 cgcactggta caccgtaatg gagttaccga atctcttgaa cccgtgtata tcagttcggg
    19321 gagtgtgcag aatgctatct gcacggtacg agcatttatt accgtggcgg ctgaaaccct
    19381 tctgcaggaa gatgcactat acaggacgga tgatggcata ttctgaaatt accgcagcga
    19441 caccatccat aagcgtcttc actgagggat atgtcgatga cttcgcttcc cgctaactgt
    19501 ataccagtaa gcaacacatc attacctgtc gccatagact acccagcagc gctggcactt
    19561 cgccagatgg ctctcgttca ggacgaactc ccgaaatacc tgctggcgcc ggaagtcagc
    19621 gccctgctcc actacgtgcc ggatcttcac cgcaagatgc tgctggcgac gctctggaac
    19681 acgggcgcgc gaattaacga agcgctggcg ctgacaaggg gggatttttc gcttgcgcct
    19741 ccgtatccgt ttgtgcagct tgcgaccctg aaacaacgga cggaaaaagc cgccaggacg
    19801 gcaggaagaa tgcccgccgg tcagcagact caccggctgg ttccgctctc cgacgcctgg
    19861 ttcgtcagcc agctgcagac gatggtggcc acactgaaaa tccccatgga acggcgtaat
    19921 aaacgaacag gcaggacaga gaaagcgcgg atctgggaag tgacggacag aacggtcagg
    19981 acctggattg ggcaggcggt tgccgccgct gccgctgacg gtgtgacgtt ctctgtcccg
    20041 gtcacgccac atacgttccg ccattcctat gcgatgcaca tgctgtatgc cggtataccg
    20101 ctgaaggtcc tgcagagcct gatggggcat aagtccatca gctcaacaga ggtctacacg
    20161 aaggtgtttg cgctggatgt ggctgcacgg caccgggtgc agttttcgat gcctgagtcc
    20221 gatgctgtca caatgctgaa aaacagacac gcataagtca caaatcataa ttatgaattg
    20281 tgatttattc tataaaagaa gagaccactg caatatgtga tctcttgtat gcaagggtgc
    20341 ttaaacagtg tgaattcaca agtgtgattt cataagtaat aacttcttga ttattacgtt
    20401 agtgttttta agtattgctc aagggctact ctgacgatgg cacttacatt ttttggatct
    20461 ttaccattgc gtttattcct caatgccaga tcttctattg tgacaagcat gctttcacca
    20521 agagagattg tcgtccgaca ttgtttttct ggttcgggtt tttccggcgc gccgtatggt
    20581 ttatccgcaa ggcgctgagc cagagcctct gcttgttcta ccgtaactgc agctctgttt
    20641 aatgcttgct ggctgggttt cttcaccatg ggccaaatac ctcattcatt aaatgttcaa
    20701 tttctgctct ggctgctgta ttgttcgttt ctactacacc ggtgccgttg ctcatgcaat
    20761 cgcgatagac ttttctgaaa caaataactg aatccaggac ttgaattgtt gggaattctt
    20821 caagatattc aaggaattct tttctctcat ttcccctcaa aaccggattg gttgttgcca
    20881 tactttgtaa acaataaacc ttaagttccg ggttaaggtt acgcattgcg tctatctgtt
    20941 gttctagttc tgtaagagta tccagatctg gttgagaaca ctgtagtggc gcaattattt
    21001 gatgtgcgac aactccactc gttatgaatt cctttgagtt tcttcctgcg acatccacta
    21061 ttacatcatc gaacttttca ttcagagcct gtagtgtttg tgtcaggtta tcaaatttct
    21121 caacaagggt tattgctggt aacagtcctg cagcttctcg ttctgcatga tgttttgcgg
    21181 ctgttcgttg tagatctccg tttaacaggc agacttccct tcctttattt gcgagagcaa
    21241 tagccaggtt tgatgcaatg gtagttttcc ctgaaccacc tttgttccca ccaactacag
    21301 taatcattgc aacctcataa atgtgatgtg tgaagtatga tgatattttg acacggtaac
    21361 ctgatgtgtc aacatgcatt catatttgtg aagtatgaaa tatgaagtat gtcatatgat
    21421 ttaataaata ttacttgtgt tttgtgattt cataactatg atggtactca aattgagtat
    21481 taccttgcaa ccagaaacag tgatttactc agattgagta tttaaggggc tatagatgaa
    21541 cgtatactgc gatgatggtt caacaacaat caagttggca tggaatgaca atgggaaaat
    21601 ctgtaagtcg ttgtcgcaaa actcatttcg tcatggatgg aaggttgacg ggttagggat
    21661 tcgccagaca tttaactatg aactggatgg taaaaaatac acgtatgatg aagttagcaa
    21721 ccaatctatt cttacgactc atattgagta tcaatatacg gatgttaacc tgttagctgt
    21781 gcatcacgct ttactgaata ctcatgctgc aggcagccag ctgtcgtcct gccagatttc
    21841 ttccagcaga gacaggatac gttctttatc cttgtcgttg cgggctcctg tgacagatac
    21901 agacggggcg ctgccctggt ctatacgcag ccagatttcc gggtagtgtg gtttcagacg
    21961 ttgttcgatt tcattctgta gcgcctgaag tgtgcctgat ttgaggtttt ttgtgctctg
    22021 gcggtcgaaa agaatttcaa tgcggatcat gttctcatct cccttttctt ttgctgtatg
    22081 tttatacagt attctaataa ctgaatccgc acacagtcaa atgtcattat tgtgactaaa
    22141 aaacgctcaa ttctctgagt gtgtggctgt atgcctgctg tgggctgctg tgtccagctt
    22201 atccacagca ttttgtgcac ggttctgtgg acaaaatacc tggttaccca ggccgtgccg
    22261 gcacgttaac cgggctgcat ccgatgcaag tgtgtcgctg tcgccggcct cctcacccgg
    22321 tcacgtttcg tcgttcctcc tccacgcgct gcggcttcgg ggccgcacct gcattcgtat
    22381 gcggtcgccc ggtaacaggt gcggcacggc ctgatggagg ccgcatgtga gaggagaatt
    22441 cccatgccaa actggtgctc gaatcgtatg tatttttctg gtgaaccggc acagatcgct
    22501 gagattaaac gactggccag cggtgcagtt acaccgcttt atcgccgcgc cacaaatgaa
    22561 ggtattcagc tgtttctggc cggaagtgcc ggacttctgc agatcactga aaatatccgc
    22621 tctgaacagt gccccggagt gacggctgcc ggacgtggtg ctgtatcgac ggaaaatatc
    22681 gcgttcaccc gctggcgacc gtttccacat ggtgaacggt gcaaactggt ttgaccgtac
    22741 cgtgagtgct gatgccgcag gcatcatcct gacctccctt gtgattaacc gccagttgtg
    22801 gctgtaccac gacagcgggg atgcaggact gacccagctt taccggatgc gcgatgcgca
    22861 gttgtggcgt cacatcgaat ttcaccccga atgcaacgcg atttacgcgg ctctggactg
    22921 attaacggac ggggcggcaa tgccgcccct gtaaaggaga cgagaacatg tactgtactg
    22981 ttaaagaaat tatccgcgaa gtgctggata cagacgtgcc ggacagtgaa tgcgtttttg
    23041 ccgtggtgct gacccgtggg gatgtgcgtc acatatccgg gacgtgttac tggaagacgt
    23101 ggcgcagcgg aatatcccgc tctcgcataa aaaactgcgc agggcgctga aggccatcac
    23161 ccgttcagaa agctatctgt gtgccatgaa agccggtgcc tgccggtatg acacggaagg
    23221 gtatgtgacg gagcatattt ctcaggagga ggaagcgtat gcggcagcgc gtctggataa
    23281 aatccgccgc cagaaccgga taaaggcaga acttcaggcc gtgcttgatg agaaataaaa
    23341 aaaagcctcc atcagagagg aggcagggaa ataaggctta aaaggaaata acttccgtac
    23401 ccgaaaggaa gccaattcag tagattattt aacaccataa tactgacggg ttaaagagga
    23461 gtgataaaga tgaatggatt cagaaacagt tcacggaacg gtcaggtctg gcgctggcag
    23521 cgtgccggaa gccgggccgt tattctggaa gtcagtggac gctggatgga agcggcagaa
    23581 gcatggcgac gggctgccag tgttgcaccc cggacagact ggcagcagtt tgcccgaaaa
    23641 agggctgaac actgccaccg gcgctgccgg ggaagggtgt aacgaaaaaa gcagccttca
    23701 ttactccagg tattcagaag gctgaataat gcgtgagatt tgcttttttt aatatccggt
    23761 ctggcagtat gaccagtcag cattcattat cacaggatta ttgctgtatt aagtaatagc
    23821 gtgctgtttt gtgattgatt tcataaaaca agacgtcgtt taatatgcgc ctgaatattc
    23881 acgagggaag tctttctttt tatatggtgt tcccggaaat aaaatatttt ttgtcattgc
    23941 tcaccctgtt atgaacaaaa ggtaaaaaca tggaagaaaa taatactagt acagataaaa
    24001 acaccctgac agcccttatt gattatgaaa actgtggcag cctgaaaaat atttcccttg
    24061 agcagtatgg ggaactgatt atttttgtcg ggccacagca gaacgtcgtt gttcttcctg
    24121 cagactcttt tcctgaaggt gcgagaatca ctatcaggca ggtgagtggt gtgtccagga
    24181 ataatgtcga ttttcatctt gttctggagc tggggcggat aagctgttgt gcagcaggga
    24241 aagataaaac ataccacatc atttcatcgg ataagggata tgacggtgtt atcaggacgc
    24301 tgaaagaagc cggtattacc tgcatccgtc gtgatgtacc ggaaagtatt gctgctgccg
    24361 gtgagtctgg tgcagacaaa agtaactgcc tgaaacaacc ttctgttcct gaaagtgata
    24421 tcgtcagccg ttgggcagga aagctgcagc agatagccgg cagcggagcc ggtaatatgc
    24481 ctgtgtccgt cgaaacattt aataattatt taaagtcaca catgcgtgac gaatggaaca
    24541 aaacccgggc agtggcgata aggaatgaac tggttcatcg gggagtcaca gaagtcaggg
    24601 agaaaaagat aatctggaaa aaggcgaaaa aggagaataa agcatgaggt atcgcagctg
    24661 tactcagata aaaacagccc ccgttgcggt tgcggagcga agggggttga aaaggggaat
    24721 taaggatgat tgttattatg gtgaaccaga aggttatccc tgtctgtaag ctgaatcgga
    24781 ctaaacgaca taatacctgt aacatcactg aaacatcagc ggtatatgtg gtgtgtatga
    24841 atatcccggc agcattaatt cttctgtgag gaaatgcatt gataagaaac agaataagtc
    24901 ggtggtaagg aaaatgaatt tattaattca gtatccgttt atcagataaa aaagggaaag
    24961 agtatggtga aactgattat ttttattatc acaataatac tgacaactaa aggggggaca
    25021 ggaaaatgaa tggattcaga aacagttcac ggaacggtca ggtctggcgt taccagagtg
    25081 ccggaagccg ggccgttatt ctggaagcgg cagaagcatg gcgacgggct gccggtgttg
    25141 caccccggac agactggcag cagtttgccc gaaaaagggc tgaacactgc caccggcgct
    25201 gccggggaag ggtgtaacga aaaagcagcc ttcattactc cagggattca gaaggctgaa
    25261 taatgcatga gatttgcttt ttttaatatc cggtcgggaa gtatgaccgg acagttctga
    25321 gtagcacaga gttttttccg tattaagtcg tgattttata ttttgtgatt aatttcacaa
    25381 aataaggtgt tattcagtgt gtgctgcaat attcaggatg catgaaatac aggtgctatt
    25441 tatctgatat ggagaataac atgaggaaat atattccact ggtattattt atcttttcat
    25501 ggccggtatt gtgtgcagat attcatggac gggttgttcg tgtactggac ggtgatacca
    25561 tcgaggggct ttgttgaata aatcagattt cgggtaagtc tcccccgtag cgggttgtgt
    25621 tttcaggcaa tacgcacgct ttcaggcata cctgctttcg tcattttgtt cagcgctcgt
    25681 accagggcca tagcctccgc aacctgacca tcgtagtcac gcagcgtcag tgaacccccg
    25741 aacagctgtt ttacccggta catcgccgtt tccgctatcg agcgacggtt gtaatctgtt
    25801 gtccatttcc accgcgcatt actcccggtc attcgctgat tagccactgc acggttacgg
    25861 tctgcatatt caccgggcca gtaacccgcg ccttttcggg gtgggataag cgcgctgatt
    25921 ttcttacgcc gcagttcatc gtgacagagc cgggtgtcgt aagcgccgtc tgccgatgct
    25981 gccctgattt ttctgtgagt ctgccggata agacccggga aggcttctga gtccgtcaca
    26041 ttgttcagcg acaggtctgc acagatgatt tcatgtgtgt tgctgtcaac tgccagatgc
    26101 agcttacgcc agatacggcg gcgttcctga ccatgctttt tgactttcca ctcgccttca
    26161 ccgaagacct tcagcccggt ggaatcaatt accaggtgtg cgatttcacc ccgggtgggc
    26221 gttttgaaac tgatattaac cgactttgcc cgcctgctga cacagctgta atccgggcag
    26281 cgtagcggaa cgttcatcag agaaaaaatg gaatcaataa agccctgcgc agcgcgcagg
    26341 gtcagcctga atacgcgttt aatgaccagc acagtcgtga tggcaaggtc agaatagcgc
    26401 tgaggtctgc ctcgtgaaga aggtgttgct gactcatacc aggcctgaat agcttcatca
    26461 tccagccaga aagttatgga gccacggttg atgagggttt tattgtaggt gggccagttg
    26521 gttattttga acttttgctt tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc
    26581 tgatccttca actcagcaaa agttcgattt attcaacaaa gccccatcga ggtcatggac
    26641 tcacggaagg cggtgcggat caggctggta aatattgatg caccggaaaa gaaacaggat
    26701 tatggccggt ggtcgacaga catgatgaaa tcactggttg caggaaaaac ggttaccgtc
    26761 acatattttc agcgagatcg ttatggtcgt attctcgggc aggtttatgc accagacgga
    26821 atgaatgtta atcagtttat ggtccgggcc ggggctgcct gggtatatga gcagtacaac
    26881 acagatcccg tgctgcctgt ctttcagaac gaagccagac aacaaaaacg tgggctctgg
    26941 tcagatgctg atccggttcc cccctggata tggagacacc gtaaataaag cgctggcaat
    27001 cttatcattc atctgttctc agcgggagta aaaatggcaa aaacaaaaca ggaatggctg
    27061 tatcagttac ggcgatgttc atcagtcaat acgctggaaa aaattattca taaaaacaga
    27121 gacagtttat caaccagtga acgtgaatca tttaattcag cagcagacca ccggttagcg
    27181 gaattaatca ccggaaagtt atatgacagg ataccaaaag agatatggaa atatgtaagg
    27241 taataattca gcataattaa ttaccaggag cagataaaca atgaagctga ttatttttat
    27301 tctcattgtt ctgattattg cagcgttact tatccggatt atcctcagaa gtgtaaatca
    27361 gcattctcct ttgctgatgc aattgcatgc ggcaggtata cggaccggtg atgcagagcg
    27421 aatactgtcc agcggtgaat actggcaacg tcagaagacg ctgctgacag aaagggaagt
    27481 cagttttatg aaaggactgt tcagaattgt ggatatgaag cggtggtatc tgtgtccgca
    27541 ggtacgggtc gcggatatcg tccagctgaa cgagaatatc cggccacgat cacgccagtg
    27601 gtggcagtta ttcaggatgg tgtctcagtg gcatgttgat gtggttatcg ttgagcggcg
    27661 ttcgttcagt attgttgcag cagtagagct ggatgatgcc agccatttac gaccggaacg
    27721 cagacgccgg gatattcttc tggaagaggt tctgaggcag gctggtattc cgttgctcag
    27781 aagccacgat gccagaaaac tgctgcagat gaccggagaa tggctgaata caacaggggc
    27841 tgatcagcag tccccggaac atcgtagctg acgccttcgc gttgctcagt tgtccaaccc
    27901 cggaaacggg aaaaagcaag ttttccccgc tcccggcgtt tccataactg aaaaccatac
    27961 tatttcacag tttaaatcac attaaacgac agtaatcccc gttgatttgt gcgccaacac
    28021 agatcttcgt cacaattctc aagtcgctga tttcaaaaaa ctgtagtatc ctctgcgaaa
    28081 cgatccctgt ttgagtattg aggaggcgag atgtcgcaga cagaaaatgc agtgacttcc
    28141 tcattgagtc aaaagcggtt tgtgcgcaga ggtaagccta tgactgactc tgagaaacaa
    28201 atggccgctg ttgcaagaaa acgtcttaca cacaaagaga taaaagtttt tgtcaaaaat
    28261 cctctgaaag atctcatggt tgagtactgc gagagagagg ggataacaca ggctcagttc
    28321 gttgagaaaa tcatcaaaga tgaactgcag agactggata tactaaagta aagactttac
    28381 tttgtggcgt agcatgctag attactgatc gtttaaggaa ttttatggct ggccacgcca
    28441 taaggtggca gggaactggt tctgatgtgg atgtacagga gccagaaaag tgaaaacccc
    28501 gataatcttc agcaagtttg gcgactaaga gaagattaac ggggccatct aaaaaccgta
    28561 tagccaacaa ttcagctatg cggggagtat agttatatgc ccggaaaagt tcaagacttc
    28621 tttctgtgct cactccttct gcgcattgta agtgcaggat ggt
//
