LOCUS       NZ_CP048343             1458 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain 142 plasmid p142_F, complete sequence.
ACCESSION   NZ_CP048343
VERSION     NZ_CP048343.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951921
            Assembly: GCF_010365385.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: 142.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010365385.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:31:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,271
            CDSs (total)                      :: 5,153
            Genes (coding)                    :: 4,934
            CDSs (with protein)               :: 4,934
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 86 of 219
            Pseudo Genes (incomplete)         :: 146 of 219
            Pseudo Genes (internal stop)      :: 39 of 219
            Pseudo Genes (multiple problems)  :: 44 of 219
            Pseudo Genes (short protein)      :: 1 of 219
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048343.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="142"
                     /isolation_source="environment"
                     /db_xref="taxon:562"
                     /plasmid="p142_F"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            complement(670..1110)
                     /locus_tag="GZS12_RS26015"
                     /old_locus_tag="GZS12_26015"
     CDS             complement(670..1110)
                     /locus_tag="GZS12_RS26015"
                     /old_locus_tag="GZS12_26015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000031238.1"
                     /translation="MSKDVERITQINDQGEIIGGFVAVIRPKQKSAFQRHFTMNQDALR
                     ILAKELTGEQFKVLMLMLADLDYENFIQIAQADIAETLGMQKTNVSRAVRALLDVGVIF
                     EGPKVGRSKTYRLNEQFGWKGTVTNHKKALKNGFSVIQGGRI"
                     /name="hypothetical protein"
     Typing          177..437
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.13"
                     /coverage="100.0"
                     /evalue="1.84e-104"
ORIGIN
        1 atgtgcaaat gtaataccgt cacccgcgac gatattccgt ttgccatctg gtcagagctt
       61 cgccccgaca ccccgtatca gtgcgtacgt gctcaatgtg gtttcctgtt ctcttccgct
      121 tcctcgccca ctcgctcact aacgttcggt cgctggggct gcggcgagcg gtgccacctc
      181 tcgcatgctg tcggtgattc cgctgttgtt ctttcgggga tgagtcgtca gatagccctt
      241 caaacgcgct cagaagctct cagacgcacg aggcggggtt taaatgcccg tcagggcatg
      301 gaaggcgcga aaaacgctta cagagcgttt caggaggtca ggcgcgccga tacccctagc
      361 caggggcttt caggtcgcca ggaaccgcgc cagccccctg caaagcccat ttgcacggta
      421 caagcagcca ctcggttggt aggtggtcgt agaccgtcgg tgttacagcg atgggggtct
      481 gggggtctcc ccagacggtt tccggttgcg ctcaaggcgc tgccggaacc gtggcggctc
      541 ggtcgttctg gagatcgggg agcaaaaaca cgcaagggat tttgaggcgg caaaagagca
      601 atgcaggata gacaatccac tagaaattgc atgcaatgtt ctgtggattg tccgcctgcc
      661 gctggctctt taaatccggc cgccctgaat gacactaaag ccgtttttga gtgccttttt
      721 gtggttggtt acggtgcctt tccaaccaaa ttgttcattc agccggtatg tttttgatct
      781 gccgacttta ggtccctcaa agatcacccc aacatccagt agcgcgcgaa cggcgcgact
      841 aacattcgtt ttctgcatcc ctaaagtttc agcaatatct gcttgggcga tctgaatgaa
      901 gttttcgtaa tccagatctg caagcatcag cattaagacc ttgaattgct ccccggttaa
      961 ttccttagcc aagatccgta atgcgtcttg attcatggtg aaatgcctct gaaatgctga
     1021 tttttgttta gggcggataa cggcaacgaa gccgccgata atctcaccct ggtcatttat
     1081 ctgagtgatt ctctcgacat ccttactcat ctttgaaccc tatttgagtt atcaataatg
     1141 ataacagtgt tatcattatt gataactatg tcaactgtaa cccttagatt ttattaatgt
     1201 ttttattctc ctttctgtat ctttatatta ggcgtctaac gcattgattc cattagatat
     1261 ttatcctagc tgctctttgg ggtgtcgggg cgaagccctg acccagtcac gtagcgatag
     1321 cggagtgtat actggcttac tcctctgcaa gcctcgtcag ccatgatttt tgtccgattt
     1381 ctcgctctcc acgttcaaca gctccatcat gtttttgccg tccgttcacc gttaaaacgc
     1441 ctcagaaatc aggacagg
//
