LOCUS       NZ_CP048341             7939 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain 142 plasmid p142_D, complete sequence.
ACCESSION   NZ_CP048341
VERSION     NZ_CP048341.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951921
            Assembly: GCF_010365385.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: 142.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010365385.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:31:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,271
            CDSs (total)                      :: 5,153
            Genes (coding)                    :: 4,934
            CDSs (with protein)               :: 4,934
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 86 of 219
            Pseudo Genes (incomplete)         :: 146 of 219
            Pseudo Genes (internal stop)      :: 39 of 219
            Pseudo Genes (multiple problems)  :: 44 of 219
            Pseudo Genes (short protein)      :: 1 of 219
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048341.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="142"
                     /isolation_source="environment"
                     /db_xref="taxon:562"
                     /plasmid="p142_D"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            complement(join(7605..7939,1..1258))
                     /locus_tag="GZS12_RS25935"
                     /old_locus_tag="GZS12_25935"
     CDS             complement(join(7605..7939,1..1258))
                     /locus_tag="GZS12_RS25935"
                     /old_locus_tag="GZS12_25935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842142.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_001009226.1"
                     /translation="MNFIEKYIITFNKCNLFFIILLSTISSFLFVLFGYLIKTVIDNKD
                     SIGEINSLLIFIACFLSIRFLMPAGYSISEYLTQKTNIELSVKLREQVIDNIQNSHQEH
                     FLKKNKGELNKVIESMLSSASSLFYTICSDVVPLLIQMIGIIITICISVNTLIAIEFII
                     IMAIYIIFVIKMTQRRFPMMKSVALSSKFASGRMFDMMHMYPMDKAFHTTDKSRKRVIQ
                     AVNTHSEKQRKVNNEFFLFGISSAFLSVIFSSLIILSAYWMFLHGRASRGSIIMLATFL
                     FQVFLPLNRIGYLFRQIKMARTEIDLYCAEINDIKKTNYTDKQYLHVKDNICNIEIYNK
                     SFNHKLILTKGVVTFITGENGSGKTTIAKILSGNITTEENTIKINGIQQEKANSPFVNV
                     LYVPQDLDLMPGTIEENILHYSGINNLNTIKKLLHRFKFDKPLDYEIKGYGHNLSGGQK
                     QKLGVSLTSGKNVDLIIFDEPTKGFDSLGIKIISDYIKEENKEKHIIVISHDEQLINNI
                     PKAQIIDITKHEV"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(1297..1995)
                     /locus_tag="GZS12_RS25940"
                     /old_locus_tag="GZS12_25940"
     CDS             complement(1297..1995)
                     /locus_tag="GZS12_RS25940"
                     /old_locus_tag="GZS12_25940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000939065.1"
                     /translation="MLQHKMNSSSYAKVHNVSSLEDIMSYHNDDVLLKFRKEWNVTPEE
                     ADDIFNETKKFIWLASTCLTECYNIKVHEQLQIIDEMWHTFIQFTDAYTSFCEKYLGAY
                     LHHYPNTNDMLKNEIRHVNEHGITFQEYRFNEYKNQIEKIAFYLGHETVAKWYGDYAVR
                     YSIKNINTIRIPKESISSDSYIEKVKSITHLPAAEFVKIIMRKDVWNDNGSVCGCSGKG
                     CGAGCSCNSR"
                     /name="hypothetical protein"
     gene            complement(2543..2701)
                     /locus_tag="GZS12_RS26775"
     CDS             complement(2543..2701)
                     /locus_tag="GZS12_RS26775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071529495.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            complement(3360..3551)
                     /locus_tag="GZS12_RS25950"
                     /old_locus_tag="GZS12_25950"
     CDS             complement(3360..3551)
                     /locus_tag="GZS12_RS25950"
                     /old_locus_tag="GZS12_25950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3616..3939
                     /locus_tag="GZS12_RS25955"
                     /old_locus_tag="GZS12_25955"
     CDS             3616..3939
                     /locus_tag="GZS12_RS25955"
                     /old_locus_tag="GZS12_25955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955993.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4148..>4579
                     /locus_tag="GZS12_RS26555"
                     /pseudo=""
     CDS             4148..>4579
                     /locus_tag="GZS12_RS26555"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4617..5135
                     /locus_tag="GZS12_RS25965"
                     /old_locus_tag="GZS12_25965"
     CDS             4617..5135
                     /locus_tag="GZS12_RS25965"
                     /old_locus_tag="GZS12_25965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673203.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            5142..5375
                     /locus_tag="GZS12_RS25970"
                     /old_locus_tag="GZS12_25970"
     CDS             5142..5375
                     /locus_tag="GZS12_RS25970"
                     /old_locus_tag="GZS12_25970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137439.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            6086..6325
                     /locus_tag="GZS12_RS25975"
                     /old_locus_tag="GZS12_25975"
     CDS             6086..6325
                     /locus_tag="GZS12_RS25975"
                     /old_locus_tag="GZS12_25975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="WP_000703021.1"
                     /translation="MKISDIEKNIYKGLAEFISQKIRDEHDICLGQFESEELLDEILKR
                     VIPVIYNVAIDDAIKSVRNVGDRLEEELDMRKVV"
                     /name="DUF2164 family protein"
     gene            6322..6684
                     /locus_tag="GZS12_RS25980"
                     /old_locus_tag="GZS12_25980"
     CDS             6322..6684
                     /locus_tag="GZS12_RS25980"
                     /old_locus_tag="GZS12_25980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="WP_000181070.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVIY
                     KKHQLRKIDMRPARILPSIERYEYDVVMREFKGIACVSGKAEIKQNDGNILMAFIENWI
                     KVNGGWKMQFISYEKI"
                     /name="DUF4440 domain-containing protein"
     gene            complement(6709..7602)
                     /locus_tag="GZS12_RS25985"
                     /old_locus_tag="GZS12_25985"
     CDS             complement(6709..7602)
                     /locus_tag="GZS12_RS25985"
                     /old_locus_tag="GZS12_25985"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_001061104.1"
                     /translation="MNNLIKKEIIEKFKKYNFQKYPFVFTDVNYKNLINWNDLNKLLEK
                     DILHYPRVRMANDNFPEIRGYKGFIRYTYSQTGDRTPHINRHQLYKCLRDGATLIVDRC
                     QSFFESVDESRLWLSKELECTCSANLYAAFTATPSFGLHFDNHDVIAVQIEGIKKWKVY
                     NPTYSYPLEDERSFDYLPPNTSPDYEFDITPGQAIYLPAGYWHNVTTQSKHSLHISFTV
                     IRPRRLELFKTLFDELKNNPYMREPIEHGDSLSDKEKIKTIITNAINSFDIQFAENMLK
                     AQSRTYRYKKIKLEHI"
                     /name="cupin domain-containing protein"
     Typing          3205..3284
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.78"
                     /coverage="92.0"
                     /evalue="8.94e-33"
     Typing          complement(2630..3172)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.679"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          3584..3933
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.872"
                     /coverage="99.0"
                     /evalue="9.42e-67"
     Typing          3930..5479
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.787"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatgtaatat attttcctct atagttccag gcatgagatc tagatcttga ggaacgtata
       61 agacattaac aaaaggagaa tttgcttttt cttgttgtat cccattgatt tttattgtgt
      121 tttcttctgt tgttatattg ccagagagaa tttttgcaat agttgttttt cccgaaccat
      181 tctcaccggt tataaaggta acaacgcctt ttgttaatat taatttatgg ttgaatgatt
      241 tgttgtatat ttcaatattg caaatattgt cttttacatg aagatattgc ttgtctgtgt
      301 aatttgtttt ttttatgtcg tttatttcgg cacagtataa atcaatttct gttcttgcca
      361 tttttatttg tctaaataga taaccaatgc gatttaatgg gagaaaaact tggaataaaa
      421 aagtggcaag cataataata ctaccacggc ttgctctacc atgaagaaac atccagtatg
      481 ctgataggat aatcagacta ctaaaaatga ctgaaagaaa agcggagctg ataccaaaca
      541 ggaaaaattc attatttact tttctttgtt tttctgaatg tgtatttaca gcttgaatta
      601 ctcgtttacg acttttatcc gtggtatgga aagctttatc cataggatac atgtgcatca
      661 tatcaaacat tcttccagat gcgaatttgg agctaagtgc aactgatttc atcattggaa
      721 atctacgttg tgtcattttt atgacaaata ttatatagat tgccattatt attataaact
      781 caatggctat taatgtatta acacttatac aaattgttat gattattcca atcatttgta
      841 ttaataatgg tactacatca gaacatattg tataaaataa tgatgatgca gaagaaagca
      901 tgctttctat aaccttattt agttctcctt tatttttctt taaaaaatgc tcttggtgag
      961 aattttgtat attatcaata acttgctctc taagctttac agataattct atatttgttt
     1021 tttgagtcaa gtattccgat atgctatatc cagcaggcat aaggaatcta attgataaga
     1081 aacatgctat aaatattaat aatgagttta tttctcctat tgaatcttta ttgtcaataa
     1141 ctgtttttat taaatatccg aatagaacga aaagaaaact acttatagtt gataataata
     1201 taataaaaaa caaattacat ttattaaatg tgatgatgta cttttcaata aaattcaatt
     1261 tataatcctt aaagcccgta tattacgggc tttatgttat ctagaattac atgagcaccc
     1321 agcgccacat cctttaccgc tgcaaccaca aacagaacca ttatcattcc aaacatcttt
     1381 tcgcattatt atttttacaa attctgcggc tggaaggtga gtgatactct ttactttttc
     1441 gatgtaagaa tcactggata tggattcttt tggaattctt atagtattaa tgttctttat
     1501 gctgtatctt acagcataat caccatacca ctttgcgaca gtttcatgac ctaggtaaaa
     1561 agcgattttt tcaatttgat ttttatattc gttaaaacga tattcttgga atgttatacc
     1621 atgctcatta acatgcctta tctcattttt tagcatgtca tttgtatttg gataatggtg
     1681 gaggtaagca ccaagatatt tttcacaaaa gctggtataa gcatctgtaa attgaataaa
     1741 cgtatgccac atttcatcta taatttgtaa ttgctcgtga acttttatat tgtagcattc
     1801 cgttaggcat gttgaggcta gccagatgaa tttctttgtt tcattaaaga tatcatcagc
     1861 ttcttctggt gttacattcc attctttacg aaattttaga agaacatcat cattgtgata
     1921 actcatgata tcttctaatg agctaacatt atgaactttt gcataagaac tgctgttcat
     1981 cttatgttgt agcataacac ccccaattat atatttgaat gtagatgaga atagttatca
     2041 tttgttatgg tattgttaaa tttgtgcttg tgtcaatatg cctaattcta taaatgagat
     2101 agtaatcaag ttttttaatg gttataggat ccattagtaa tacaaggttt ttgcagccag
     2161 tttttacggg taggggctgc aaggaaaaca aaattttccc actctaagag aagagaattg
     2221 agttggttga atgagtggat gcatctaggg ccgctaattc accgagtgta aaggctgtac
     2281 gaaatgtgtt gcagtccgat aatagccccc tccattgacg ttttacacat gtgttgtagg
     2341 ttccaggagt gggcggatct caaaaagatc aaaggatctt cttgagatcc tttttttctg
     2401 cgcgtaatct gctgccagta aacgaaaaaa ccgcctgggg aggcggttta gtcgaaggtt
     2461 aagtcagttg gggactgctt aaccgggtaa ctggcttggt ggagcacaga taccaaatac
     2521 tgtccttcta gtgtagccgc agttaggcca ccacttcaag aactcttaat atctcaatcc
     2581 accttgtcca gttaccagtg gctgctgcca gtggcgcttt gtcgtgtctt accgggttgg
     2641 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2701 cacagcccag cttggagcga acgacctaca ccgagccgag atacctacag cgtgagctat
     2761 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2821 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2881 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt atgatgctcg tcaggggggc
     2941 ggagcctatg gaaaaacgcc aacggcgcag ccttttcctg gttctcgttt tttgctcaca
     3001 tgtttctttt ggcgttatcc cctgattctg tggataaccg catctccgct tttgagtgag
     3061 cagacaccgc tcgccgcagc cgaacgaccg agtgtagcga gtcagtgagc gaggaagcgg
     3121 aagagcgccg gaacgtgcat tttctcctta cgcatctgtg cggcatttca catcggacat
     3181 ggtgcgcttt ccatacaatt cgtactgatg ccgcatagtt aagccagtat acactccgct
     3241 atcgctacgt gactggttca gggcttcgcc ccgaaacccc ctgacgcgcc ctgaggggct
     3301 tgtctgctcc cggcatccgc tcacagacaa gctgttaccg tctccgggag ctgtatgtgt
     3361 cagaggtttt caccgtcatc cccgaagcgt gcgaggcagc tgcggtaaag ctcgtcggcg
     3421 tggtcgtgaa gtgattcaca aatatcggcc tgttcatctg cgtccagttc gttgagtttc
     3481 tccagcagcg ttaatgtctg gcttctgata aaacgggcca tgttaagggc ggttttttcc
     3541 tgtttagtca ctgatgcctc cgtgtaaggg ggatttctgt tttatggggt aatgataccg
     3601 atgaaacgcg agaggatgct caccatacgg gttactgatg atgaacatgc ccggttactg
     3661 gaacgctgtg agggtaaaca gctggcggta tggatgcggc gggtctgcct gggtgagccg
     3721 gttgcacgct ccgggaagct gccgacactg gcaccgccgt tactgcgtca gctggccgcc
     3781 atcgggaata acctgaatca gacagcgagg aaggtgaaca gcgggcagtg gtcttccggt
     3841 gatcgggttc aggtgttggc tgcgctgatg gccatcgggg aggagctgcg tcggctgcgt
     3901 ctggccgtca gggaacaggg cgcgcgggat gattgttaaa tttcatgcca ggggaaaagg
     3961 cggtggcagt ggtccggttg attacctgct gggaagggag cgaaaccggg aaggggcaac
     4021 ggtgcttcag ggtaatccgg aagaagtccg ggaactcatc gatgccacgc catttgcgaa
     4081 gaaatacacg tcaggtgttc tgtcgttcgc ggagaaggag ctgccgccgg gaggacgtga
     4141 aaaggtgatg gcgagctttg agcgtgtact gatgcccggt ctcgaaaaga atcagtacag
     4201 catcctgtgg gtggagcacc aggacaaggg acggcttgag ctgaattttg tcattccgaa
     4261 catggagcta cagaccggaa aacgcctcca gccgtactac gaccgggcag acaggccgag
     4321 aattgatgcc tggcagacgc tggtgaatca ccattacggg ctgcatgacc ccaacgcccc
     4381 ggagaaccgc cggatactga ctctccctga taacctgccg gaaacgaaac aggcgcttgc
     4441 agagagcgtc acacgcggga tagacgctct ttatcatgtc ggggagataa aaggtcgtca
     4501 ggatgtgatt caggcgctca cagaggcagg gcttgaagtg gtcagggtga tgcgaagcag
     4561 tatcagcatt gcagacccga acggtgggaa gaatatcagg ctgaaaggag cattttatga
     4621 gcaatctttt acagacgggc gcggagttcg agaaaaagct gaaagagaga gccgaatcta
     4681 cagagacaat gctgaacgac gagttcagca agctcgaaaa atctgtaagc aaggctgtga
     4741 catcaaacga gacgaaaatc aaagacgcta tagccctgtt cacagcttcg acagaggaat
     4801 cactgaaaaa acaccgggaa ggggtgaaag aggcgatgat gcagcacagg aaggacgtgt
     4861 taaagctggc agggaatacg ggcatgatgt tactggggat ggtccttttc ctgtttaccg
     4921 tgagtggcgg gacgctctgg tatcttggcg ggatgataca ggcgaacctg gaagaaatca
     4981 ggatacagga agaaacattg cagaaactga acgcgaagac atggggcgtg gagtttgtgc
     5041 aggacgggag aaggaaattt ctggttatcc ctcaggggaa atcggcaacg gtgattccct
     5101 atcaggggaa ggactgggta caactgacgg agtgacacag agtgacggag ctggaaagac
     5161 atttgctgaa cgccttagag caactgcaac aggactatat gcagcggctg aacgaatggg
     5221 agagcgcctt cgtggaattg cagaaaatgt tttcgcttac gcaacgggac aacgcgatgc
     5281 tgaacgagcg ggtcatgcag ttgagtcagc aggtgcagca cttgagcgag cagacagaac
     5341 gcttgagcca gttatacagc gagaacagga gataagggat gaacggctga tacaggaacg
     5401 cgaacatgag ttatccctgg agcgtgagcg ccagttggaa atacaggaac ggacacagga
     5461 tggcccttcg ctgggatggt aatcagagac aggctaaaat gttgtcagcg cgtttccggt
     5521 gcattttttg ggataatggt tggttcgggg gcaccgggaa cgcgcttcag tcagaccttg
     5581 ctgaaaacac aggaacgcgt taaaacgcat acgggcgcga ttctggtgtg tcttttgttg
     5641 ctgtgttttg cctctattgc cagatcgcgc tcattcccct gaacttctgt tttcccgctc
     5701 cggtccccgt cttcttcgct tccgaaagca tgaaaaaaat ccgtttcagc tgtctctggc
     5761 tggcaattcc tgcgtgattc acatggctgc atagctatgc atgtggttaa aatttaccgg
     5821 gtgcgatcgc ggcagttttt cgggtggttt gttgccattt ttacctgtct gctgccgtga
     5881 tcgcgccgaa cgcgtttcag cggtgcgtac aataagggat tatggtaaat tatcttgttc
     5941 cttagagaat gatttgaggt ggtatcctgc acttatttaa atatgcttgc ttgggaaatt
     6001 ccaggttgca gtatggttga gttgtaattt tcctatgata ggaaaaatgt tatattatta
     6061 atttatttgt gaggcttcat aaagaatgaa aatttctgat attgaaaaaa acatatataa
     6121 aggactagct gaatttattt cacaaaaaat cagagatgaa catgatatat gccttggaca
     6181 gtttgaatca gaggaattac ttgatgagat tcttaaaaga gtaatcccag tcatatataa
     6241 tgtggctatt gatgatgcta taaaatcagt aaggaatgtt ggggatagat tagaggagga
     6301 attagatatg aggaaggtag tgtgactaat tttaaatcag acttaaatct aataagaaaa
     6361 gaatggtata atgcatttta ttcaggtaat actgaaatgc tagattactt ggaagctgat
     6421 tggtttattt caacaaatgg tcaaaaagtt atctataaaa aacatcagtt acgaaagatt
     6481 gatatgcgtc cagcgaggat tttacctagc atagaaagat atgaatatga tgtcgtaatg
     6541 cgtgagttta aaggcattgc ttgtgtttca gggaaagctg aaataaagca aaatgatgga
     6601 aatatattaa tggcttttat tgagaattgg ataaaggtta atggtggatg gaaaatgcag
     6661 tttatatcgt atgaaaaaat ctaaggggaa gcccccttag attaatggtt atatatgttc
     6721 aagttttatt tttttatagc gataagttct gctttgagct ttaagcatgt tttctgcaaa
     6781 ctgaatatca aaactattta tagcattagt tattatagtt tttatttttt ctttatcaga
     6841 taatgaatca ccatgctcta taggttcccg catatatgga ttgtttttta attcatcaaa
     6901 taatgtttta aatagttcta atcgacgagg tcttataact gtaaaagata tatgaagaga
     6961 gtgtttgctc tgagtcgtaa cattgtgcca gtatccagca ggaagatata tggcctgacc
     7021 tggggttata tcaaactcat aatctgggga ggtattaggt ggtagataat cgaagcttct
     7081 ttcatcttcg agagggtatg aataggtagg gttgtaaact ttccattttt ttattccctc
     7141 aatttgaaca gctattacat catgattgtc aaaatgaagc ccaaagcttg gtgttgctgt
     7201 aaatgcagca tatagattag cactacatgt acattctaac tctttagata accatagtct
     7261 gctttcatct acagattcaa agaatgactg gcatcgatct actataaggg ttgcgccatc
     7321 acgtaagcat ttatatagct gatggcgatt tatatgtggt gttctatcgc ctgtttggct
     7381 atatgtgtat cttataaatc ctttataccc cctaatttcg ggaaagttgt catttgccat
     7441 tctaactcta ggatagtgca atatatcttt ttcaagcaat ttattaagat cattccagtt
     7501 gattaagttt ttataattaa catccgtgaa tacaaacgga tatttctgga aattatattt
     7561 cttaaatttt tctatgattt ccttttttat aagattattc atttttaaac ctcatgtttt
     7621 gtgatatcta taatctgtgc tttaggtata ttatttataa gttgttcatc atgagatata
     7681 acaatgatat gcttttcctt gttttcctct tttatataat cactgattat ttttatacca
     7741 agtgaatcaa acccttttgt cggttcatcg aaaataatca agtctacgtt ttttccagat
     7801 gtaagagata ctcctaattt ttgtttttgt ccaccagata aattatgtcc gtaacctttg
     7861 atctcataat ctagtggttt gtcgaactta aatctgtgta gcagcttttt aattgtattt
     7921 aaattattaa tgccggaat
//
