LOCUS       NZ_CP048336             4064 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain 10 plasmid p010_F, complete sequence.
ACCESSION   NZ_CP048336
VERSION     NZ_CP048336.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951922
            Assembly: GCF_010365425.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: 010.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010365425.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:31:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,097
            CDSs (total)                      :: 4,976
            Genes (coding)                    :: 4,695
            CDSs (with protein)               :: 4,695
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 281
            CDSs (without protein)            :: 281
            Pseudo Genes (ambiguous residues) :: 0 of 281
            Pseudo Genes (frameshifted)       :: 98 of 281
            Pseudo Genes (incomplete)         :: 187 of 281
            Pseudo Genes (internal stop)      :: 62 of 281
            Pseudo Genes (multiple problems)  :: 58 of 281
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048336.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="10"
                     /isolation_source="environment"
                     /db_xref="taxon:562"
                     /plasmid="p010_F"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            complement(406..660)
                     /gene="mobC"
                     /locus_tag="GZS13_RS25160"
                     /old_locus_tag="GZS13_25160"
     CDS             complement(406..660)
                     /gene="mobC"
                     /locus_tag="GZS13_RS25160"
                     /old_locus_tag="GZS13_25160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            839..2986
                     /gene="mobQ"
                     /locus_tag="GZS13_RS25165"
                     /old_locus_tag="GZS13_25165"
     CDS             839..2986
                     /gene="mobQ"
                     /locus_tag="GZS13_RS25165"
                     /old_locus_tag="GZS13_25165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_053920598.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELKTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(3235..3936)
                     /locus_tag="GZS13_RS25170"
                     /old_locus_tag="GZS13_25170"
     CDS             complement(3235..3936)
                     /locus_tag="GZS13_RS25170"
                     /old_locus_tag="GZS13_25170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(662..838)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          839..1197
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3236..3936)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          753..2983
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.856"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaagctccac ctttcacccc caaaagctcc acctttcacc cccaaaagct ccacttatct
       61 tgccagaaca tctttaaaat catggtgtta caagacctaa aaaggtttta aaaaaaaaga
      121 tctaaaaata agatcttaaa aacttaacgg cgagcctgtg gataacttgg ggtgctgaaa
      181 acaaaaaacc aataaaaaca atgcgttgca ataaagcaga attgcaagtt tcgaatacac
      241 cccctccaac cctgtggaaa cccagtattc atgcgggttc agcgcatatc acctgccgaa
      301 aaaccgtgcg tatgtgctca acctggcgac tgattcactt ccgcttcctc gcccactcgc
      361 tcgctaacgc tcggtcgctg gggctgcggc gagcggacga tacaatcact gttctggcag
      421 cgcctccagc tcgttaatca gcggcagtat gcgcgtcaca tcagcctgtc gtgtcacctc
      481 tgccttcagg aactggagta accaggcacg caactgcacc tcctccggct ttctgacctt
      541 tgccagcaat gcagcaccca caacaacctt ctgcgctgtg tccaggctcc ggctcttcgc
      601 cttcaggcgc tgtaatctcg cctcagcatc agcaatcttc tgttcgagcg ttctgctcat
      661 gatgtaactc cgtgcgcgat gaaaaatcgc attttagcgc gtcactggta gtttaaaaac
      721 tgagctggca taatgcacgg caaatcacga agtgcgcact tatacaatct ccactccgtt
      781 tcgattgtgt gcgccctgcg gggctgaaag aaaactacaa aaaggcatta cggcagaaat
      841 ggcgatttat catctcagca tgaaaattat ctccagaagc agcggctaca gcgccgtagc
      901 ttccgctgcc tatcgttccg gttcactgat gcttgatgag cgtaccggac tgactcacga
      961 ctacacccgc aaaagcggtg tggctgaggc tgtgattctg acccccgcca ccgcccctgc
     1021 ctggtgtacg aaccgtgccg aactctggaa tgccgttgag aaggccgaac gccggaagaa
     1081 ctcacaactt gcgcgggaaa ttgaactcgc catcccccac gaactgccgc aggatgccgc
     1141 acgggaaacc gttctcgcct ttgtcagaga aaactttgtc agtcagggca tgattgctga
     1201 tgtggcgttc catcacatgg ataaaacaaa tccccatgcg cacatcatgc tgaccacgag
     1261 ggctatcgga cctgctgggt tcagcggaaa ggtcagggac tggaacgacc ggacacatgc
     1321 cgagacgtgg cgggcatcat gggctgacca tgcaaacagg gcactggcga acgccggtta
     1381 tcaggaagag atagaccatc gttcatacga gcgacagggg cttgagaaaa cgcctggtat
     1441 tcacctcgga aagtctgcct gtgcgatgga aacacgcggt atcgaaacag aacgcggcga
     1501 acagaatcgt ctgattaaca ggcttaacct ggaaatacag atttcccgca ccgaactcag
     1561 gaacggaggg ctgacagagc ctgcccgccg tgtggctgac cttctgaata tcgggcttcc
     1621 tgacaacgca acttcgtaca cactacgtga cattatcgaa gcgttgccga aggacagtaa
     1681 ctcggcatgg aaactcacca gtaatgccat gtcgatgatg gcagatatgc aggcgacgcg
     1741 cagacgctgg gaagagctga agaccgaacg aaaggaagcg atgagccatg ctgccagcct
     1801 gaaaaaatca tcgccgtttt cgtccggttt ttcccgtatt cccctgatgc aatgggcggc
     1861 accggaatac cgtagtgagc agggaaaaat ccagtctctg agccagcaaa tggaaaaatt
     1921 acgacagcat taccggaagg tggagaaaca ggacattccc gcgagtcaga tggcatttga
     1981 aaaacagtgg cagcagtggg gggaatccgg ccttgcagct ctcagagccc agttgcttaa
     2041 acgtgaagag gaattgcgcc ggaaggaaca ggaagatact gaacgtagtc gcgcggagca
     2101 gctcaggaaa aatgacaatg ccgttatgga ttcaggggtg ctgagagcag tcgttacggg
     2161 gtatggcgag gctcccatgc ctggtaacgg ggaaatgacc tgttaccttc tgcttcacaa
     2221 ccggaacgga gaatatacgc tctgggggaa cgagctggag aaataccgca ccagaatctt
     2281 tgagagtgtt gatttaatgc gtgacaggag tggttatatc tgtgaccgaa gcgaaatcgg
     2341 ccaacgtccg ccactgcaac gtgtttattc ctctgcaacc tttgagcaac tgctgacgca
     2401 ggtttgccag acatggccgc aacatacccg caatctccgc cagccgaaga catggccgga
     2461 gtctttctgt cttggagaag accgccagcc agccatgcca tcaccagcgg caaggaaggt
     2521 ggattttatg cagggacgtc tgccaccgac gctgatgccg gtcatgagtt ctgtcgaccg
     2581 ggaaacccgc cagcttcagc ttttgctggt catgggcgtg gatgactctc tgggcggtgt
     2641 tatccggctt aatggtacgc tgtatcccgc gtttgccgtt ccctcagccg ataacaggca
     2701 tctggtcatc agtgcgctga ccgataaggg gttacggtat gcagggtatg gtgttgccgt
     2761 aaatcacgat gcagacagcc acatatcacc tgcaccagaa ctgatgcagt ttcatctgaa
     2821 aacccgtgag gcaccgcttt ttgctgccgt caacacacca gaaaaacagc cggatcatct
     2881 gttccgctcg ctgggtttta atcgtacctg ggaggaatgg cagcgcgagg aagatgcacg
     2941 aacacacacc accgagagga gacatgatcg cggttggtca cagtgaatgt gaacaatatt
     3001 ttgttttgtt taacatcgtg ttatgttcgt cgatgttcat cgacagggat gaatgcaaga
     3061 agcacactca cataacgcaa gcatagtttt gtttttccgc cccccaaaaa ttggggggtc
     3121 ttttttcgct gcatcagagc gtttttgacg caatttttca cttcaatagt accttcgcac
     3181 tactaacaca aaaaaaacgc agcagggagc gttttaggtg ccttaaaatc gattttagat
     3241 cgtcatcttg atctgtttgt cttccttgaa gcgaaaatgc agggctacaa ccgtgcgccc
     3301 ctttttgatc gtctcaacag ttacaaccag atcgcttttt tcgttcagtt cggcgatggc
     3361 tggcttgatt atcatcttat tcaagtcttt gaattgttga tatttatctt caacggcaag
     3421 catggttcgg aaatcatcga tagctatcgt tctgtcaccg gtgctgcgga actgctggag
     3481 cagctcgtaa atgcggatgc tgtaggtgct ggaaaggctg gagacgttct tcacgactac
     3541 ccgcgtaaac tgccccttga gctgtgtcag atacggcatg atggcatcgg agaaggtgat
     3601 atctactttc gcctcacctt tgaaatactg cgcccggtac tgcacccagc ggaactcttc
     3661 tcgtttttcc tcgttcttga tgattactga cctgtcccag aggcgatcta tcgcttcctt
     3721 gagcctagct tcagcgtgct gccgtcccat gtcaggaaag gcatcatgaa actctgcagc
     3781 cgtgatggtc atggtttttt gttcatccgg cgaaggggaa tcttctccgg attttaggcg
     3841 gctgatacag accagcagga agcgctgttc ctgcaaagtg agcttgtagc ttgcctcgat
     3901 aagggcgttt gatttgtaag ctatcagatc tgacatttcg cttgctcgtt gctattggtg
     3961 gcgttttttt actgtacgcc acttatcata tgggtggaag tttttttttt gcaaccccct
     4021 ttttctgtgg attacacccc caaaagctcc acctttcacc ccca
//
