LOCUS       NZ_CP048335             4065 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain 10 plasmid p010_E, complete sequence.
ACCESSION   NZ_CP048335
VERSION     NZ_CP048335.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951922
            Assembly: GCF_010365425.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: 010.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010365425.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:31:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,097
            CDSs (total)                      :: 4,976
            Genes (coding)                    :: 4,695
            CDSs (with protein)               :: 4,695
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 281
            CDSs (without protein)            :: 281
            Pseudo Genes (ambiguous residues) :: 0 of 281
            Pseudo Genes (frameshifted)       :: 98 of 281
            Pseudo Genes (incomplete)         :: 187 of 281
            Pseudo Genes (internal stop)      :: 62 of 281
            Pseudo Genes (multiple problems)  :: 58 of 281
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048335.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="10"
                     /isolation_source="environment"
                     /db_xref="taxon:562"
                     /plasmid="p010_E"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            complement(254..487)
                     /locus_tag="GZS13_RS25130"
                     /old_locus_tag="GZS13_25130"
     CDS             complement(254..487)
                     /locus_tag="GZS13_RS25130"
                     /old_locus_tag="GZS13_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137430.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLVEQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(494..1054)
                     /locus_tag="GZS13_RS25135"
                     /old_locus_tag="GZS13_25135"
     CDS             complement(494..1054)
                     /locus_tag="GZS13_RS25135"
                     /old_locus_tag="GZS13_25135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067489.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENILNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1089..1769)
                     /locus_tag="GZS13_RS25940"
                     /old_locus_tag="GZS13_25140"
                     /pseudo=""
     CDS             complement(<1089..1769)
                     /locus_tag="GZS13_RS25940"
                     /old_locus_tag="GZS13_25140"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1732..2055)
                     /locus_tag="GZS13_RS25145"
                     /old_locus_tag="GZS13_25145"
     CDS             complement(1732..2055)
                     /locus_tag="GZS13_RS25145"
                     /old_locus_tag="GZS13_25145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2123..2314
                     /locus_tag="GZS13_RS25150"
                     /old_locus_tag="GZS13_25150"
     CDS             2123..2314
                     /locus_tag="GZS13_RS25150"
                     /old_locus_tag="GZS13_25150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2982..3095
                     /locus_tag="GZS13_RS25710"
     CDS             2982..3095
                     /locus_tag="GZS13_RS25710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001427889.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGN"
                     /name="hypothetical protein"
     gene            3457..3849
                     /locus_tag="GZS13_RS25155"
                     /old_locus_tag="GZS13_25155"
     CDS             3457..3849
                     /locus_tag="GZS13_RS25155"
                     /old_locus_tag="GZS13_25155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001218243.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRALQKLQI
                     QEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGNNA
                     ETQSTHYQPYPYDKVSIWGPFPHNLT"
                     /name="hypothetical protein"
     Typing          complement(2400..2487)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.753"
                     /coverage="100.0"
                     /evalue="1.48e-35"
     Typing          2498..3054
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.583"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(148..1742)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.872"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2115..2317)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.235"
                     /coverage="100.0"
                     /evalue="5.02e-12"
ORIGIN
        1 acatcgcggg atgaggctga ggaatgagcg cgatctggca aagaggcaaa acacagcaac
       61 aaaaacgaca cgccagaatc gcgcccggat gcgtttttaa cgcgttccgg taccatctgg
      121 caacctcccg gaacaactca ccgtcatatc cctattgacg ggccacgcca tacccgtgct
      181 tcccgttcct gctcttcatg ccaggaccgc gcacgctccc gttccaggcg tgcctgcctt
      241 tcctgttcat cccttatctg ctgttcgtga taaataaccg actcaagtgg tccacctgct
      301 cgactaatct ctgcacctgc tgactcaagt cgtcgcactg ttccctcagt tgcccgttct
      361 cctgtcgtgt cagctcgaac atatgctgca aatccgtgaa ggcgctctcc cagtctttca
      421 gccgctgcgt atagtcctgc tgcaattgct ctaaggcgtt cagtaagtgc atttccagct
      481 ctgtcatact cacttactcc ctgaccagtc ttactgcgtt cttcttctcc accgtccagt
      541 tgttttcccc cttcaccccg gacggcaata ccagaaattt cccgttcctg ccctcgtgat
      601 acgtcacacc ccatgttttt tcccggagtt tcgccagcgt ctcttcctgg tccctgatag
      661 ccaggatgtt cgccgcaatc cggctttcct gccactgaat cagcccccat gacgccagaa
      721 aaatcccgcc cgtgacgcaa agcgccgtca gcgacgggta cagtatccgc cctttgacca
      781 gcttccagag cagctcttcc tgccgccggg ccagttcgtt ctctgtggcg ctgaactgcg
      841 cgttcacggc actgttcagc gtctccagtt gttctttcac cgctgctgtg tgtgcgctga
      901 tagcgtctct gattttctgc ccgtttaagt tcagttccct gtctacagac gcttcgagct
      961 tcctgaactc gctgttcagt atgttctctg tagagacggc acgctctttc agtttcttct
     1021 cgaagtctgt ccccatttgt aaaagattgc tcatacagcg cccctttcag cctgagatta
     1081 cgcccgccct ccgggtcggc gatactgata ctgctcctgg ttgtcctcac aacctcaaaa
     1141 cctgccgctg taagcgcctc agtgacatcc tgacgcgttt ttagcgctcc ggcatggtaa
     1201 agagcctcca gtcccctcgt aatcgcttct gcggcctcct gtttcgcttt cggcagatta
     1261 ttcggggtga caagtgtccg cctgttctcc ggtgcgttcg ggtcgtgcag cccgtaatgg
     1321 tgattcacca gtgtctgcca ggcattgatt cgcggacggt ccgctcggtc gtaatagggc
     1381 tggagccgtt ttccgttcgc cagctccata ttcgggatga caaaattcag ctcaagacgc
     1441 cccttgtcct ggtgctccac ccacaggatg ctgtactgat ttttttcaag accgggcatc
     1501 agtacccgct caaagctctc catcaccctt tcacgctctc ccggtggcag ggtctgctct
     1561 gcaaaagaca gaacccccga ggtgtatttt ttcgcaaacg gcgtggcatc gatgagttcc
     1621 cgcacctctt cgggagcacc ccgcagaact ctcgcccctt cccggttacg ctcccggccc
     1681 agcaggtaat caaccggacc actgccaccg ccttttcccc tggcatgaaa cttaactatc
     1741 atcccgctct ccctgtttac ggacctcatc cctcagctca ctcagttcac gtccgatggc
     1801 catcagtgca gccaccacat gaacccggtc atgccccgac cactgtccgc tgtttatctt
     1861 ccgggctatc tgattcaggt tattgccgac cgaagcgaac tggcgcaaca gcggcggtgc
     1921 cagtgtcgga agacctgacg ttttcgatgg cggtgccccc aggcagacct tacgcatcca
     1981 tgacgcaagt tgttttccct cacaacgtgc cagcagccgc gcatgttcct catccgtgac
     2041 ccgtatcgtg agcatccttt cgcgtttcac cggtatcatt aaaaacctcc gacagactcc
     2101 ccacacatgg agaaacagaa ctgtgactaa acaggaaaaa accgccctta acatggcccg
     2161 cttcatcaga agccagacgc tgaccctgct ggaaaaactg aatgaactgg acgccgacga
     2221 ccaggctgac atctgcgaag cgcttcacga ccacgctgac gagctttacc gcagctgcct
     2281 cgcacgcttc ggggatgatg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     2341 agctggcctg tgagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcaggttt
     2401 tagcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     2461 cttacttatg cggcatcagt gcggattgta tgaaaagtgc accatgccgg gtgtgaaatg
     2521 ccgcacagat gcgtaaggag aaaatgtagc ttccggcgct cttccgcttc ctcgctcact
     2581 gactcgctac gctcggtcgt tcggctgcgg cgagcggtat cagcgcactc aaagacggta
     2641 acacagttat ccacagaatc aggggataaa gcaaaaagaa acatgtgagc aaaaaacaag
     2701 agcccgaaaa aggccgcgcc ggtggcgttt ttccataggc tccgcccccc tgacgagcat
     2761 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggacttaa agataccagg
     2821 cgtttccccc tggtggctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2881 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgttggt
     2941 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3001 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3061 acttaacgcc actggcagca gccattggta actagacaag gtggattgag atattaagag
     3121 ttcttgaagt ggtggcctaa ctgcggctac actagaagga cagtatttgg tatctgtgct
     3181 ccaccaagcc agttaccttc ggaagaaaga gttggtagct ctagattccg gcaaacaaac
     3241 caccgttggt agcggtggtt tttttgtttg caggcagcag attacgcgcc gcaaaaaagg
     3301 atctcaagaa gatcttgaat cactctatga aaattttgtt ttatagccgt atttgttgat
     3361 gctataagcc agagaccttc tatgagaata gacatatcac atcaaacacg gcataccccg
     3421 ccaaacatgc ttcctcggga acaaaattgt gtagcaatgg cattatctgc gtgctttaga
     3481 caacaactaa accctgtggt aaacagctta ttaaaagaga gaataataca ctcccctaaa
     3541 gaattggaac atgacaacgc agtaataaga gccctgcaaa aactacaaat tcaagaagtt
     3601 tgtaacagca ccctttggga gacagccaaa caacaactac ttcaaaaatc ggacggaaga
     3661 tattttgcca tcaattctaa acacttagcc tttccaggcc caggagaatc tcacgccttc
     3721 tgttgcatca aatacaaaaa tgcaattggc attaacggaa acaatgccga aacccaatcg
     3781 acccactacc aaccctatcc ctatgataaa gtcagtatat ggggaccatt ccctcacaat
     3841 ttaacgtaat atcttaattg tacgcaccgc tgaaatgcgt tcagcgcgat cacggctgct
     3901 gacaggtaaa aatagcaaca aaccacccga aaagctgccg cgatcgcacc tgataaattt
     3961 taaccgtatg catagctatt cagccatgtg aataacgctg gttttgcctg cgtaaacctc
     4021 atgacactgt tttttttcca tcttttcagt tgatgacata cgcag
//
