LOCUS       NZ_CP048334            13907 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain 10 plasmid p010_D, complete sequence.
ACCESSION   NZ_CP048334
VERSION     NZ_CP048334.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951922
            Assembly: GCF_010365425.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13907)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13907)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: 010.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010365425.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:31:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,097
            CDSs (total)                      :: 4,976
            Genes (coding)                    :: 4,695
            CDSs (with protein)               :: 4,695
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 281
            CDSs (without protein)            :: 281
            Pseudo Genes (ambiguous residues) :: 0 of 281
            Pseudo Genes (frameshifted)       :: 98 of 281
            Pseudo Genes (incomplete)         :: 187 of 281
            Pseudo Genes (internal stop)      :: 62 of 281
            Pseudo Genes (multiple problems)  :: 58 of 281
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048334.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="10"
                     /isolation_source="environment"
                     /db_xref="taxon:562"
                     /plasmid="p010_D"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            104..1648
                     /locus_tag="GZS13_RS25045"
                     /old_locus_tag="GZS13_25045"
     CDS             104..1648
                     /locus_tag="GZS13_RS25045"
                     /old_locus_tag="GZS13_25045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="WP_201360865.1"
                     /translation="MKNYDVFYGGIFKTITDYKLLKIQKDIKNNMTALVSSFKWVGIGQ
                     LVKIATQLVSLFYLTHLIPPSSYGLLAMSSVVMNLASIFNDMGTGAAVIQRQEASIGFY
                     NYIYRINIFTGLFVMLLVIITSPLIVVYFNTPELYPVLFLLAFSFPISSLTITHKAKLE
                     KEMNFKVIIAIEIFSSLTGMVLAIVLANLGFGVYSLIFQTLTTISISTICFLKVSGLSL
                     SLRIKNISHSDKSGVIGFSGYLLSFNLINYFSRNLDSILIGRFFSSAILGVYSIAYRIM
                     LFPLQSLTLVFSRVFLPHLSKNINNTNNTNKQDYLNALKLILSLSAPLMLGLSATSFSF
                     TRIFFDPKWHLLGDLLHWLAPTAIIQSALSTTGSVFMAYGKTRWLFFLGCIGAFLMGLS
                     FIIGVHFDIKTLVYLYFFANLINSLPAFILLKNILKFSYIELVNVFFRTISPAFLMFLL
                     IKVWQHFSFFKNASIDFSFNVALGIFSYIVFYTLFNFNEVVRTGVIHKLRCMTKLK"
                     /name="lipopolysaccharide biosynthesis protein"
     gene            complement(<1741..2005)
                     /locus_tag="GZS13_RS25050"
                     /old_locus_tag="GZS13_25050"
                     /pseudo=""
     CDS             complement(<1741..2005)
                     /locus_tag="GZS13_RS25050"
                     /old_locus_tag="GZS13_25050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /name="IS1-like element transposase"
     gene            complement(2150..3026)
                     /locus_tag="GZS13_RS25055"
                     /old_locus_tag="GZS13_25055"
                     /pseudo=""
     CDS             complement(2150..3026)
                     /locus_tag="GZS13_RS25055"
                     /old_locus_tag="GZS13_25055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946055.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(3225..3509)
                     /gene="nadS"
                     /locus_tag="GZS13_RS25060"
                     /old_locus_tag="GZS13_25060"
     CDS             complement(3225..3509)
                     /gene="nadS"
                     /locus_tag="GZS13_RS25060"
                     /old_locus_tag="GZS13_25060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_032242301.1"
                     /translation="MSFFDELKASLEEAVEIKNGTKAPARVTRYELADVRAIRENLNIT
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFDELAAH"
                     /name="nadS"
     gene            complement(3511..3843)
                     /locus_tag="GZS13_RS25065"
                     /old_locus_tag="GZS13_25065"
     CDS             complement(3511..3843)
                     /locus_tag="GZS13_RS25065"
                     /old_locus_tag="GZS13_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_032242302.1"
                     /translation="MSHAIEFIETTMFTRQIKQIATDDEIKELQKELIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSITDAEKAELKKLTQ
                     RLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3988..4224)
                     /locus_tag="GZS13_RS25070"
                     /old_locus_tag="GZS13_25070"
     CDS             complement(3988..4224)
                     /locus_tag="GZS13_RS25070"
                     /old_locus_tag="GZS13_25070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_032242304.1"
                     /translation="MTEMERQLLSALESLQTSYEQQKQAWQDSYSSLQRMFETTSQALE
                     RNDRVCLNLSNQVNALHEQVERLSSDLSRLTRS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4620..5833
                     /locus_tag="GZS13_RS25075"
                     /old_locus_tag="GZS13_25075"
     CDS             join(4620..4911,4911..5833)
                     /locus_tag="GZS13_RS25075"
                     /old_locus_tag="GZS13_25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_139371347.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQSEYDSQWATICSIAPKIGCTPE
                     TLRVWVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFGEGGVRP
                     PLEKMMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEI
                     QRVYDENHKVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAV
                     AAGDRVNRQFVAERPDQLWVADFTYVSTWRGFVYVAFIIDVFAGYIVGWRVSSSMETTF
                     VLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAES
                     INGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGN
                     DDLAA"
                     /name="IS3 family transposase"
     misc_feature    4901..5017
                     /locus_tag="GZS13_RS25075"
                     /old_locus_tag="GZS13_25075"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            5799..6242
                     /locus_tag="GZS13_RS25080"
                     /old_locus_tag="GZS13_25080"
     CDS             5799..6242
                     /locus_tag="GZS13_RS25080"
                     /old_locus_tag="GZS13_25080"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202752352.1"
                     /translation="MLPSETMIWQPEFTDKTLSRKPGAVQFSYLKAMYHQSKIQAELKA
                     QIHEQTTVNAICHHPESIEIIAVCSTDAYYRKRKDAAFLTTCSVLMRTLKDESVPMVLR
                     KTAWRLLNERYQRIKLNQAYRIENFLLVADFEYALEEHDELAE"
                     /name="hypothetical protein"
     gene            complement(6672..6806)
                     /locus_tag="GZS13_RS25085"
                     /old_locus_tag="GZS13_25085"
     CDS             complement(6672..6806)
                     /locus_tag="GZS13_RS25085"
                     /old_locus_tag="GZS13_25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072003336.1"
                     /translation="MHEPPVQSDRCVLSGNYRLESNPERHAKTPLAAAIGKQDVREMQ"
                     /name="replication initiation protein"
     gene            complement(7990..8307)
                     /locus_tag="GZS13_RS25095"
                     /old_locus_tag="GZS13_25095"
     CDS             complement(7990..8307)
                     /locus_tag="GZS13_RS25095"
                     /old_locus_tag="GZS13_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="WP_032242326.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAVNVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="lipoprotein"
     gene            9392..9541
                     /locus_tag="GZS13_RS25325"
     CDS             9392..9541
                     /locus_tag="GZS13_RS25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_167776965.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAQVTEENLHALVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            10001..11229
                     /locus_tag="GZS13_RS25110"
                     /old_locus_tag="GZS13_25110"
     CDS             join(10001..10316,10316..11229)
                     /locus_tag="GZS13_RS25110"
                     /old_locus_tag="GZS13_25110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="WP_088895425.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQ
                     LFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYG
                     RGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLR
                     IHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVPPSKR
                     AHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQ
                     DVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACN
                     GLSDNRCLEI"
                     /name="IS3-like element IS2 family transposase"
     gene            11260..11628
                     /locus_tag="GZS13_RS25115"
                     /old_locus_tag="GZS13_25115"
     CDS             11260..11628
                     /locus_tag="GZS13_RS25115"
                     /old_locus_tag="GZS13_25115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020936656.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNGFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     gene            11713..12696
                     /locus_tag="GZS13_RS25120"
                     /old_locus_tag="GZS13_25120"
     CDS             11713..12696
                     /locus_tag="GZS13_RS25120"
                     /old_locus_tag="GZS13_25120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_151166972.1"
                     /translation="MKKKFSIIVPVYNVEKYLRECIQSVLDQDFNDYEIICVNDGSTDG
                     SLDIILSFDDQRIKVINQENKGLSVARNVAIESSTGEYIWFLDSDDKISHGSLKLIDKI
                     TNEKNYGIIFFNTHVFFDTPELKKKGWHDYKRSTFGEMDALTFFEKEMTNKKLIVSACC
                     YVFRRELLNGNAFIPHIYHEDNHFIATLIFDNYSTICLSLDEALYLRRVRNDSIMTSKK
                     SMRHIIGYSTCIDELTKYKKTKGIQNKSFDRFVNGMVLSLFSIAETIKTKEAMNYVARY
                     KKHPDLSFKDRALINYPLIKKTYLKLKNGKVFNYLRNGIINKKSNP"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(13155..13852)
                     /locus_tag="GZS13_RS25125"
                     /old_locus_tag="GZS13_25125"
     CDS             complement(join(13155..13603,13603..13852))
                     /locus_tag="GZS13_RS25125"
                     /old_locus_tag="GZS13_25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223368647.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     Typing          9184..9357
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.143"
                     /coverage="100.0"
                     /evalue="5.58e-78"
     Typing          complement(6699..7064)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.185"
                     /coverage="89.0"
                     /evalue="2.91e-169"
     Typing          complement(8709..9217)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cactattggt ggagatgatc ataatgcctt ataatcatac gggatacgaa cttataaagt
       61 cctaaagttt agctaaatcg gaatctacaa cgtagatgat agcttgaaga attatgatgt
      121 cttttatggt ggtatcttta aaaccatcac ggattataaa ttattgaaaa ttcaaaagga
      181 tattaaaaac aatatgactg cactagtcag tagttttaaa tgggttggca ttggacaact
      241 tgtaaaaata gcaacacaat tggtttcgtt attctacttg acgcacttaa tccccccatc
      301 gagctatgga ctattagcaa tgtcatctgt ggttatgaat ttagcaagca tattcaatga
      361 catgggaact ggggcggcag taattcagcg acaagaggcg tcgatcggtt tttataatta
      421 catatacagg ataaacattt ttactgggtt gtttgttatg ctgcttgtaa taataacatc
      481 cccattaatt gtcgtgtact ttaacacacc cgagctatac ccggttctat ttctattggc
      541 ttttagcttt ccaatttcca gcttaacaat aactcacaaa gcaaaattag aaaaagaaat
      601 gaactttaaa gtcatcattg caattgaaat tttctctagc cttacaggaa tggtgctggc
      661 aatagtttta gctaatttag gctttggtgt ttatagtttg atttttcaaa cactgacaac
      721 aatatcaata tccacaatat gttttttgaa ggtttccggg ttgagtttat cattgagaat
      781 taaaaatatc agccatagtg acaagtcagg agttataggg tttagtggtt atttattgtc
      841 ttttaattta ataaattatt tttccaggaa tcttgattct attttaatag gccgtttttt
      901 ctcatccgca atacttgggg tctattcgat tgcctaccgc attatgttat ttccgttgca
      961 gagcctgacg cttgtttttt ctagagtttt cttgccacac cttagcaaaa acataaataa
     1021 cactaataac actaataaac aagattactt aaatgcatta aaacttatac tatcattaag
     1081 cgcgccactg atgctaggtt tatctgctac atctttttct tttacgagga ttttctttga
     1141 tcctaagtgg cacctacttg gagacttatt gcattggttg gcacctacag cgattatcca
     1201 atcagccttg agtacaacag gttctgtttt tatggcctac ggaaaaacaa ggtggttgtt
     1261 tttcttaggt tgcattggcg cttttttgat ggggttgtct ttcattatag gtgttcattt
     1321 cgatataaaa acattggtct acctttattt ttttgcaaac ttaattaatt cactcccggc
     1381 atttatttta cttaagaata tcttgaagtt tagttacatt gaattggtaa acgtgttttt
     1441 tagaactatc tccccagctt ttttgatgtt cttacttatt aaagtatggc agcatttttc
     1501 ctttttcaaa aatgccagca ttgatttttc ttttaatgtc gcattgggta tttttagtta
     1561 tattgttttt tataccttgt ttaatttcaa tgaggttgtt aggacaggag taattcataa
     1621 gttaagatgc atgaccaagt taaagtgatt catttattaa attcattaaa tgaattggta
     1681 atgactccaa cttactgctt gtgttttatg ttcagataat gcccgatacc gacttcggcc
     1741 taattttttt aagtgtcgta aaatcgtgtt gaggacagtg cccataatgc gggatgtggc
     1801 ccggcatcca acgccattca tggccatatc aatgattttc tggtgtgtac cgggctcaga
     1861 ggcggtgtaa gtgaactgta gttgcttgct atgttttacg acagtgagag cagagatagc
     1921 gttgatgtcc ggtagtgctt ttaccgttac gcaccacgcc ttcagtagct gaacaggagg
     1981 gataactgat ggaaatggaa gccacgggag cacctcaaaa acaccattag acactaaacc
     2041 agcaagttgg catcatcaca aacagttgtt actgctggaa aaaaaacact atcaacactt
     2101 aagtaatggt gattcaataa ttaagattca gtatgtaaaa tactgaatct taattataaa
     2161 ttttcctttt tctaaaaaat gtagcgcgac taatacctaa agcaacccaa ggctccccta
     2221 gagacagcct acctcctcct gatgattttt caccaccaac ttttcctctt ttggcttgta
     2281 taagtgaaaa attttgctta ttaaatttca aaaatgtcca cttgcagata cttttcgcta
     2341 ttcccgttac ttcattctca ttcaaaggca ctgcaaattg cagattgtaa gcgcaggcgc
     2401 gttcatggca ggcttgtagc cactgttcgt actcaggcca gccctgacgg atagcgcgat
     2461 atgcccactt tctggtttta tcaaacagta tgcagttacg acctaagcca taatcggcga
     2521 cgattttttt atcgttcgca gcgttcaggt cgagtgattc ggctaaccag tccagcgtgt
     2581 agagttcagg ctgccatacg ggcaattttc cagtgactgt ggttcggatt cttgcaaatt
     2641 agccccgaat accccgtgtc agcgcccaat tttttacgta gcgcgctctc tacagcagcg
     2701 gcgtaacgta acggtttcat ttttccgtct ggcgcggtgc gtatcgaggt tttcagtgcg
     2761 tacagcaggt gcgcgtgtcc gttttcgggg ttggtgatgg tcagtgtcgg cgcgggggcg
     2821 ttgcggtcgc tccagtcaat ggctgcgccg atgcggtcaa cgtcaaatcc cagccagaac
     2881 atggcgtgag gctgattaaa ctggatgtat ttagccagaa tagcgcgctc tttccctgcg
     2941 atgcggacac caaagtgtaa atcatccgag aagtacggtt tatgcggcaa gcggtcgttg
     3001 aaaagttcga gtgctgcgtt acgcaatgtg ccctgaactt atggcgggct gttgtctggc
     3061 acggttcatg tgctaatatc cttcttaggt tttataccta gccctgattt atcatgtctc
     3121 ttccgccaag ttgaaatcat gattatcggg gctttgtttt ttctgttgcc gttaaagtta
     3181 cacgcgaacg gcctcgcctg tcgaatttct tctgtcacat cagcttaatg cgccgccagt
     3241 tcatcaaaaa actttgggtt agcctgaatg gtggccagca cttttgctgc cagtccggtt
     3301 gggttacggc gtccggtttc ccagcttttg atggtatcga ggctggttcc cagcactttc
     3361 gccatttcgc tctgagtaat attcaggttt tctctgatag ccctgacgtc tgccagctcg
     3421 tagcgggtca cacgggcggg ggctttggtt ccgtttttga tttcaacggc ctcttccaga
     3481 gaggctttca gctcgtcaaa aaaactcatc ttacacctcg tctttcagtc gttgggtcag
     3541 cttcttcagt tctgctttct ctgcgtctgt gatgctgtct ttggtgcttt tagggtaggc
     3601 catcaccaga taaatcacct ctgcggtggc aaggaagtaa atcacccgcg cactgccgct
     3661 ctttccctga ttccccgtcg ccatcctgat ctttcgcaga ccgcccgttt tctggatgag
     3721 gtcacctttg tccggtgact cgataagctc tttctgaagc tctttgatct catcatccgt
     3781 ggcgatttgc tttatctgac gggtaaacat cgttgtctcg ataaactcaa tcgcgtgact
     3841 cacggctgtc tcctgtatat tttgatgtac aatgtacacc aagaggcgtg ttctgaaaag
     3901 ccgcgttctg tgccatcaga cgttacatgg actgacctcg atagcgggtt gcttgcttct
     3961 cctgctgtaa ctgccgttcc tgctgcttta cgagcgtgtt aatcggctca gatcgctgct
     4021 caagcgttcg acctgttcgt gcaaggcgtt cacctgattg ctcaagttca gacataccct
     4081 gtcgttgcgc tccagtgcct gtgaggtagt ctcgaacatg cgttgcaagc tgctgtaact
     4141 gtcctgccac gcctgcttct gctgttcgta gcttgtttgt aagctttcta aggcgctcag
     4201 aagctgcctt tccatctcgg tcatggtttt tcctgtcctc agtgataaaa acgtgctgtg
     4261 gcgtttctgt ggtgtctgac ggggttattt cgcggtgtac ttcatcacgg tgtttccggc
     4321 tgtcgatgtg tatgtctcgg tgccatgcgc tttcttcggc agtaccacca gataggtgtt
     4381 cttctgcttg tcgtccttgt aaatcgctac ccctgcgccg ctctggggtc cgcttggaaa
     4441 aaggaaatta tcctacgcta agcgcatttt ttgcacagat ggctaccgac tgaacgtaac
     4501 gtgtgagtca tgatcagaaa tgtgttaact ctaggaattg acacgactta ctgtttgatg
     4561 gggaatgaac cgccccggga atcctggaga ctaaacttcc tgagaaagag gtaaacagga
     4621 tgactaaaaa tactcgtttt tcccccgaag tccgtcaacg ggcagtccgt atggttctgg
     4681 aaagtcagag cgaatatgac tcacaatggg cgacaatttg ttccattgct ccaaagattg
     4741 gctgtacgcc ggagactctg cgtgtctggg ttcgccagca tgagcgggat accgggggcg
     4801 gtgatggagg gctcaccacc gctgaacgtc agcgtctgaa agagctggaa cgtgaaaatc
     4861 gtgaactgcg ccgcagtaac gatatccttc gccaggcttc cgcttatttt gcgaaggcgg
     4921 agttcgaccg cctctggaaa aaatgatgcc actgctggat aagctgcgtg agcagtacgg
     4981 ggtcggaccg ctatgcagcg aactgcatat tgccccgtca acgtattacc actgtcagca
     5041 acagcgacat catccggata aacgcagtgc ccgtgcgcag cgcgatgact ggctgaagaa
     5101 agagatacag cgcgtatacg atgaaaatca caaggtatac ggtgtgcgta aagtctggcg
     5161 tcagttgtta cgggaaggta tcagagtggc cagatgcact gtggcacgtc tcatggcggt
     5221 tatgggactt gccggtgttc tccggggtaa aaaggtccgt acgaccatca gccggaaagc
     5281 cgttgccgca ggcgaccgcg taaaccgtca gttcgtggca gaacgacctg accagctgtg
     5341 ggtggctgat tttacttacg tcagcacatg gcggggcttc gtctatgtgg cgttcatcat
     5401 tgatgtgttt gccggataca tcgtggggtg gcgggtctca tcgtccatgg aaacgacatt
     5461 cgtgctggat gcactggagc aggcgttatg ggcccgtcga ccgtccggca cggtccatca
     5521 cagtgataaa ggttctcagt atgtatcgct ggcctacaca cagcggctta aggaagccgg
     5581 attactggca tcaacaggaa gtacaggcga ctcgtatgac aacgcgatgg cggagagcat
     5641 caatggtctt tacaaagcgg aggtaataca ccgtaagagc tggaaaaacc gtgcagaagt
     5701 ggaactggcc acactcacgt gggtggactg gtataacaat cgacgattgc tggaaaggct
     5761 gggccatact cctccggcag aagcagaaaa agcttattat gcttccatcg gaaacgatga
     5821 tctggcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg gttcagttta
     5881 gctatctcaa ggctatgtat catcaatcaa aaattcaggc tgaactcaag gcacagatac
     5941 atgaacaaac tacggttaat gctatctgtc atcaccctga atcaatcgag atcattgcgg
     6001 tttgttcgac tgatgcgtat taccgaaaac gtaaggacgc tgcttttctg acaacctgtt
     6061 ctgtgctcat gcgtacttta aaagatgaat ccgtaccgat ggtactacgg aaaacagcat
     6121 ggcgattatt gaacgaaaga taccaacgaa ttaagttaaa tcaggcttat aggatagaaa
     6181 atttcttgct tgttgccgat tttgaatatg ctctcgaaga acatgatgaa ctagcagagt
     6241 agtactagtg aggcgatgaa taccgacagt taacgtgcat gtgacttaaa cgtccgatta
     6301 cgtacagcat gtagacaaca tgtccgaaag taagcaaaac cattaacgga cagccggatc
     6361 atttcgtaca tcccttacgg tcaggggttc gcgctcaatg gaacgaaatc acccgttagc
     6421 ttaaaacgct taacgtagga taatttcctt tttccaagcg gacccgcaat acagcaagga
     6481 acttcttgag atcctttctg ttgcgtcgta atctcttgct ctgtaaacga aaaaaccacc
     6541 ctggcaggtg gtttttcgaa ggttagctaa tcctggcaga ttttctaacc gcggtaacag
     6601 tcttgtgcga gacatgtcac caaatctgtc ctttcagtgt agccgcactc aggccatcac
     6661 ttcaagaact cttactgcat ctctcgcaca tcctgtttac caatggccgc tgccagtggc
     6721 gtttttgcgt gtctttccgg attggactca agacgatagt taccggacaa gacgcagcgg
     6781 tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa
     6841 ctgagtgtca ggcgtggaat gagacaaacg cggccataac agcggaatga caccggtaaa
     6901 ccgaaaggca ggaacaggag agcgcacgag ggagctacca gggggaaacg cctgggatct
     6961 ttatagtcct gtcgggtttc gccaccactg atttgagcgt cagattctgt gatgcttgtc
     7021 aggggggcgg agcctatgga aaaccggcag cgccgcggcc tttcctccct cgctttatct
     7081 cgtcttcatc gccatatgcc tttgtgaata ttctccgtcc gctcgccgca gccgaacgac
     7141 cgagcgtagc gagtcagtga gcgaggaagc ggaatatctt cggcctcacg tccggcacct
     7201 tacactccgt aactgcattt tttcatagga tgcaggatag ggctaacgcc gcaaagtgac
     7261 gtcacctgac gttcctggat tacaaaggtt aaagcagcgg acaggaaatg ttttgtgccc
     7321 acagctatgc tgggttattc acaaaacagc gggacaaaat gtgtttttgg agtaccgccg
     7381 gcccgctaaa gactcctggt tagctcccgc atgtttgcta tctttttgct accattttgc
     7441 tacttttttg ctatctccag agtcttctgg tctttcggtt gctatccttt tgctactttt
     7501 ttgctatcaa aatgctacct ctcccttctt gcaataaatg accaaggcac gttagcgatg
     7561 tatacatcgc cgcgccgcaa gggtgcccct tccgaatccc gttcatcact gtgtgctgat
     7621 taatccaggt taaatccgac ccccttagca ataaatcctg ccatgcctga cggcctggcc
     7681 aacgcctcac ggtcgcacag taaactgcgc accctttcac cgttatcggt ggggttcgtg
     7741 gtggctttca gggcgctcgc cggattttag ccgctaaaat gacgtccatg cgttcgtgtg
     7801 catagccatg cagacgcgaa gaaatcatca ggcgcgtttt ttcacgatat ggacagggaa
     7861 agatgccgga aataggcagg agggcgcaga aatgccctga gggcgtctga gatggtgcac
     7921 gcaacggatg ttatggtaaa ttcagctgat tggcaggtac aaaaaaagcc ccgagggctt
     7981 ttttgtacct tattgatctg cacactttgc agccttcagg gcttccagct gatttagccg
     8041 gttagctgct tttttgcggg cttcgccttt tgccatgcca tttcctatcc caaaatcacc
     8101 caggaaacct aatacggtgc gcccgtcaaa ttcgccggtt tcctcaattt cttgttgtac
     8161 actgcgcgtt ttggcgattt ccagagctac cgctttgcaa tccattgcgg aagattctgg
     8221 accggtgacg ttcacggcct gtggatattg ctttgttgcg catcctgcca tgatcagcgc
     8281 tgccgatagg atgaatattt ttttcaaaat tatccttatt tatttaggca tagttaagag
     8341 atacccattt tatcggcagg aaatgagggt tttttagttg aattttggga ggggcaaggt
     8401 tccatattgg tcgaggattc tggagctgac cggcaacggc cagcgcggct atgggccatg
     8461 cggtggttga gcatagaaag gatcttcttg agttcctttt tttctacgcg taatctcttg
     8521 ctctgaaaac gaaaaaacca cctggggagg tggtttttcg aaggttaagt cagttgggga
     8581 actgcttaac ctggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     8641 gtagccgtag ttaggccacc acttcaagaa ctctatagca ccacctacat taactcgctc
     8701 tgctaatcct gttaccagtg gctgctgcca gtggcgcttt gtcgtgtctt tccgggttgg
     8761 actcaagacg atagttaccg gataaggcgc agcagtcgga ctgaacgggg ggttcgtgca
     8821 tacagtccag cttggagcga actgccttcc cggaactgag tgtcaggcgt ggaatgagac
     8881 aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc
     8941 acgagggagc cgccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     9001 cactgatttg agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac
     9061 ggctttgccg cggccctatc gcttccctgt taagtatttc cctggcatct tccaggacat
     9121 ctccgccccg ttcgtaagcc atttccgctc gccgcagtcg aacgaccgag cgtagcgagt
     9181 cagtgagcga ggaagcggaa tatatcctgt atcacatatt ctgctgacgc accggtgccg
     9241 ccttttttgt cctgccacat gaagcacttc actgactccc tcatcagtgc caacatagta
     9301 agccagtata cactccgcta gcgctacgtg actggttcag ggctgcgccc cgaaaccctc
     9361 taacaacctc tcagctactg gatatcaccc catgccatct gaacatgatg aacaactgga
     9421 ttcgactgat acgcagcttc ccggcgaata tgaccttttt gatttaatgg cgcaggttac
     9481 tgaagagaac ctgcatgccc tggtggattt tgggccgcct gtcggccggg aagcgttata
     9541 gctttttcgc ttacccgcgt acgtggtaat acgccggtat tacatcttat ttctttattt
     9601 tcatgtaatt acaggtggtt gtaatacgtt gcgtattaca taaataaacg gggacttcag
     9661 tttttatctt ttaattcatg caattgcgat gaaaatcgcg gttgcgtatg gtgtatgacc
     9721 atttaaggga taatgtaacc tgacttttaa agaatgaatc tatctttggc agcgcatgag
     9781 atcgataaaa agcgttccac atcaggcggt tcatgtatcc atgaaccgga aaggacaaaa
     9841 ataagacaat tgtaagacat tttttgcgtt ttactcttat tttggactat tatgagacaa
     9901 atgtaagact agactggccc cctgaatctc cagacaacca gtatcactta aataagtgat
     9961 agtcttaata ctagttttta gactagtcat tggagaacag atgattgatg tcttagggcc
    10021 ggagaaacgc agacggcgta ccacacagga aaagatcgca attgttcagc agagctttga
    10081 accggggatg acggtctccc tcgttgcccg gcaacatggt gtagcagcca gccagttatt
    10141 tctctggcgt aagcaatacc aggaaggaag tcttactgct gtcgccgccg gagaacaggt
    10201 tgttcctgcc tctgaacttg ctgccgccat gaagcagatt aaagaactcc agcgcctgct
    10261 cggcaagaaa acgatggaaa atgaactcct caaagaagcc gttgaatatg gacgggcaaa
    10321 aaagtggata gcgcacgcgc ccttattgcc cggggatggg gagtaagctt agtcagccgt
    10381 tgtctccggg tgtcgcgtgc gcagttgcac gtcattctca gacgaaccga tgactggatg
    10441 gatggccgcc gcagtcgtca cactgatgat acggatgtgc ttctccgtat acaccatgtt
    10501 atcggagagc tgcccacgta tggttatcgt cgggtatggg cgctgcttcg cagacaggca
    10561 gaacttgatg gtatgcctgc gatcaatgcc aaacgtgttt accggatcat gcgccagaat
    10621 gcgctgttgc ttgagcgaaa acctgctgta ccgccatcga aacgggcaca tacaggcaga
    10681 gtggccgtga aagaaagcaa tcagcgatgg tgctctgacg ggttcgagtt ctgctgtgat
    10741 aacggagaga gactgcgtgt cacgttcgcg ctggactgct gtgatcgtga ggcactgcac
    10801 tgggcggtca ctaccggcgg cttcaacagt gaaacagtac aggacgtcat gctgggagcg
    10861 gtggaacgcc gcttcggcaa cgatcttccg tcgtctccag tggagtggct gacggataat
    10921 ggttcatgct accgggctaa tgaaacacgc cagttcgccc ggatgttggg acttgaaccg
    10981 aagaacacgg cggtgcggag tccggagagt aacggaatag cagagagctt cgtgaaaacg
    11041 ataaagcgtg actacatcag tatcatgccc aaaccagacg ggttaacggc agcaaagaac
    11101 cttgcagagg cgttcgagca ttataacgaa tggcatccgc atagtgcgct gggttatcgc
    11161 tcgccacggg aatatctgcg gcagcgggct tgtaatgggt taagtgataa cagatgtctg
    11221 gaaatatagg ggcaaatcca aagacattat gggcggtata tggcgaattt taaccctggt
    11281 ccaagaacag ccattgcagc tgtcagggat tccaagaacg atatagaaac ctggaagaaa
    11341 aatggtattc gtagggaaga gattgtgact gtgctgaatg aacgttacgg cctgaatatc
    11401 tcactggttg cgtttgataa ggctctttac agattgagaa aaagtacaga tgaaccgtct
    11461 cttgctgatg tggattctgt gtctgctgca gaaacgaatt tctctgaagc cagtgggcct
    11521 gacaatggtt tttttctgtc cgggaatgac ttcatgcgag cagaagaaga atctcaaaga
    11581 gtggttgagt cattcagaga tagccttagc aggaagaaag gattctgatt tattcaacaa
    11641 agccgttgat aaatcgcatt ccataaataa caacttgatg ttagtataac taaatattgt
    11701 ttggagatta ctgtgaaaaa aaaattttcg ataatagtac ctgtatataa cgttgaaaaa
    11761 tacttaaggg aatgtattca atcagtatta gatcaggatt ttaacgacta tgagataatc
    11821 tgtgtaaatg atggttcaac agatggcagt ctagatatca tactgtcatt tgatgaccaa
    11881 agaatcaaag taataaatca ggaaaataaa ggcctgtcgg tggcaagaaa tgtcgccatt
    11941 gaatcatcaa ctggtgaata tatatggttt ttagatagtg atgataaaat atcacatgga
    12001 agtctaaagt taatagacaa gataaccaat gaaaaaaatt atgggattat tttctttaat
    12061 acacatgttt tttttgacac tccagaactg aaaaaaaaag gctggcatga ttataagcgt
    12121 tcaacatttg gtgaaatgga tgcattaact ttctttgaaa aagaaatgac gaacaaaaaa
    12181 ctaatagtgt cagcatgctg ctatgttttt agaagggaat tattaaatgg caatgcattc
    12241 attccgcata tatatcatga ggataatcac tttatagcaa ccctgatttt tgacaattat
    12301 tcaactatat gcctctcgtt ggatgaggcg ttatatctta ggagggtaag aaatgattcc
    12361 ataatgacgt ctaaaaaatc aatgaggcac atcattggat attcaacatg catcgatgag
    12421 cttacgaagt ataaaaaaac aaaaggcata caaaacaaga gctttgatag atttgtgaat
    12481 ggaatggttt tgtcgttatt ctctatagcg gaaacaataa aaacaaagga agcaatgaac
    12541 tatgtggcga gatataaaaa acaccccgac ctatcattta aagatagagc attaattaat
    12601 tacccattaa ttaaaaagac ttatttaaaa ctaaaaaacg ggaaggtgtt taattaccta
    12661 aggaatggca ttattaataa aaaaagcaac ccttaataaa agcaaagcga taattaataa
    12721 aggtttttct ttgttgaata aataagattt cggataggta tccccgtgac gggttgggtt
    12781 ttcaggcgat acgcacgctt tccggggctt tgttgaataa atcgaacttt tgctgagttg
    12841 aaggatcaga tcggggtcag tttggagatc ggaaattttt gtacgttaag cgcattattt
    12901 aaccaatctt ggttcgtaag gggttgattt tgcgtcagtg gctatcgctt ttaagttgtc
    12961 gctcttgcag tatcgcattt aggttatccg attaaggtca aacctctgaa aatgccgtat
    13021 agcgcgggag tacaccctat tgctataggt aagtctgttc aaaaaacagg cttaccgtac
    13081 aataattctc tatatccaaa ctaaccccag atcacgcatc ttcccgacaa cgcagaccgg
    13141 gtaatgactc caacttactg atagtgtttt atgttcagat aatgcccgat gaccttgtca
    13201 tgcagctcca ccgattttga gaacgacagt gacttccgtc ccagccttgc cagatgttgt
    13261 ctcagattca gattatgtcg ctcaatgcgc tgcgtatatc gcttgctgat aacgtgcagc
    13321 tttcccttca ggcgtgattc atacagcggc cagccatccg tcatccatac cacgacctca
    13381 aaggccgaca gcaggctcag aagacgctcc agtgtggcca gagtgcgttc accgaagacg
    13441 tgcgccacaa ccgtcctccg tatcctgtca tacgcgtaaa acagccagcg ctgacgtgat
    13501 ttagcaccga cgtagcccca atgttcgtcc atttcagcgc agacaatcac atcacttccc
    13561 ggttgtatgc gcgaggttac cgactgcggc ctgagttttt taagtgacgt aaaaccgtgt
    13621 tgaggccaac gcccataatg cgtgcactgg cgcgacatcc gacgccattc atggccatat
    13681 caatgatttt ctggtgcgta ccgggctgag aggcggtgta agtgaactgt agttgccatg
    13741 ttttacggca atgagagcag agatagcgct gatgtccggc agtgcttttg ccgttacgca
    13801 ccacgccttc agtagcggag caggaaggac atctgatgga aatggaagcc acgcaagcac
    13861 cttaaaatca ccatcataca ctaaatcagt aagttggcag cattacc
//
