LOCUS       NZ_CP048329             3789 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain 61 plasmid p61_C, complete sequence.
ACCESSION   NZ_CP048329
VERSION     NZ_CP048329.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951923
            Assembly: GCF_010365405.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: 61.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010365405.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:29:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,708
            CDSs (total)                      :: 4,588
            Genes (coding)                    :: 4,381
            CDSs (with protein)               :: 4,381
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 71 of 207
            Pseudo Genes (incomplete)         :: 123 of 207
            Pseudo Genes (internal stop)      :: 60 of 207
            Pseudo Genes (multiple problems)  :: 40 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048329.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="61"
                     /isolation_source="environment"
                     /db_xref="taxon:562"
                     /plasmid="p61_C"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            403..552
                     /locus_tag="GZS14_RS23460"
     CDS             403..552
                     /locus_tag="GZS14_RS23460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_180251257.1"
                     /translation="MPSEHDEQRDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            1949..2467
                     /locus_tag="GZS14_RS23270"
                     /old_locus_tag="GZS14_23305"
     CDS             1949..2467
                     /locus_tag="GZS14_RS23270"
                     /old_locus_tag="GZS14_23305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162676513.1"
                     /translation="MSEKAKKVGRRSLSKRRERLIKELWGDELSELNIWNRKEHDGFTT
                     IPRTLNYINRIIDYLAGAGSPVSQTYLSLWCRVFDEGFVEIRDKDALAFESGFSGQRAV
                     TTWLGRMKKLRELGLISTKPGTSGEFHYVIMLNPLYVIKKSYESNGWKRDERYNALFSR
                     MQEVGAEWE"
                     /name="hypothetical protein"
     gene            2464..3027
                     /locus_tag="GZS14_RS23275"
                     /old_locus_tag="GZS14_23310"
     CDS             2464..3027
                     /locus_tag="GZS14_RS23275"
                     /old_locus_tag="GZS14_23310"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000999222.1"
                     /translation="MNDMKAVRHRYKQRYDFAASEVVGAFDELRSLDWSLEIASLSDVE
                     MSKRWIGAVDPNGVARLPPNGGWDWVKLYNGYLKNPSAFVCAIKSEGKLCALIGGGVSD
                     SKVVTKLHYLESAPYDTALSGYALDISVTYVVTVANAMGSEYSAIYEPNSKVREIAIND
                     YGFSEDNLFGTSKDFALYLPMSRI"
                     /name="hypothetical protein"
     gene            complement(3037..3189)
                     /locus_tag="GZS14_RS23280"
                     /old_locus_tag="GZS14_23315"
     CDS             complement(3037..3189)
                     /locus_tag="GZS14_RS23280"
                     /old_locus_tag="GZS14_23315"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001341064.1"
                     /translation="MLAHAVELKKATEPCSSGTAGAFRQRVKARRAEQGRSNQQPEKLY
                     GVLVG"
                     /name="hypothetical protein"
     gene            complement(3486..3632)
                     /locus_tag="GZS14_RS23820"
     CDS             complement(3486..3632)
                     /locus_tag="GZS14_RS23820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050863271.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPERHAKAPLAAANGKRMYKRLLT
                     QSS"
                     /name="hypothetical protein"
     Typing          196..410
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.52"
                     /coverage="100.0"
                     /evalue="3.460000000000001e-56"
     Typing          complement(2..232)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.979"
                     /coverage="96.0"
                     /evalue="1.17e-49"
     Typing          complement(3346..3789)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.905"
                     /coverage="87.0"
                     /evalue="8.02e-146"
ORIGIN
        1 agtcctgtcg ggtttcgccg ccactgattt gagcgtcaga ttttgtgatg cttgtcaggg
       61 gggcggagcc tatggaaaaa cggcaacgcc gcatcctttt tccgctcttc ctgatttccg
      121 ctaagtctct ttatccagcc attcgtgagc tggcaacgcc cgccgcagtc tcacggcagg
      181 agcgcagcga ccgagtgagc gaggaggcgt catttcaccg ggatctgcat ttcgcactta
      241 cacaccggtg ctgcatttcc cggtaccaca tgaagcactt tcgtaaatta ttactgcgtt
      301 gtagcatttt aagcccgtat acactccgct agcgctacgt gactggttca gggcttcgcc
      361 ccgaaacccg ctaacaacct ttcagctact ggatatcacc ccatgccatc tgaacatgat
      421 gaacaacggg attcgactga tacgcagctt cccggcgaat atgacctttt tgatttaatg
      481 gcgcaggtta ctgaagagaa cctgcacgcc ccggtggatt ttggcccgcc tgtcggcagg
      541 gaagcgttat aacttttttc gctggtcagc gtacgtgtaa tacgccggta ttacatctta
      601 tttctttatt ttcatgtgat tacaggtggt tgtaatacgt tgcgtattac ataaataaac
      661 gggaaattca gtttttacct tttaattcat gcaattgcga tgaaaatcgc ggttgcgtat
      721 ggtgtatgac catttaaggg ataaaaaatc atcttttttg gtagggcgct ctccgtaggt
      781 taaggggtat ttggctaaaa agcgtcctat tctttgatgg tcatgcaagc atgaccatct
      841 gagcaaccaa aaactacaga taaactacaa aaaacgtttt gcattagtga tggcagacta
      901 caaaaaaact acaaaaaccg tggcggacta ctaatagact acaagaaaac tacagataaa
      961 ctacaaagta actaccggat tacctcagat ccgttccctg aaccattcgt acatcggccc
     1021 ccttcctgaa cgtgctgaaa acgggttttc cggctgtgcc ggcaacgcct cacggccacg
     1081 caataaattg cgtccccgtt caccgttttc gggtaagaag aatatcggaa acaggactta
     1141 ccccggctga atggcgtgaa tttccgcgct atgcacttgc gcgcatactc atgcatgccg
     1201 taaaaacaga gcctgcgcgt ttctggcggg ttttcgggtg gtttgttgcc tgttttaccg
     1261 gtttcccgtc agaaacgccc tgagggcctc tcaggcggtg cacgcaagag atgttatggt
     1321 aaatggtttc tgaggtgtaa gcgaaaactc gagttttgac gccattctga gtaggtaatt
     1381 tgcagacgga ataaatactg gcgagcaata agctcatcaa caaaaaatgg agatcctaag
     1441 atggatagag aaaccctata cctactcaaa acgttagacc ataacaatgc cttgcttgac
     1501 gatattatcc gaagaaagat gggtaggtac tacaacacga agattttgcg tgattcctgt
     1561 ggtgctattg aagccgaact acgacgtcga ggaatcattt aaaaagacga agcccgctta
     1621 tgtgggcttt ttcattgcgc attcatggca ccccttcctc cccccttaaa ccccctaaga
     1681 accccattca gcacagcgag cacaacgatt acattagagt acattgagaa cagcgagtac
     1741 aaaataacgc ataactaatt gaaaaataat accgtaaccg taaagaatat tacatctcaa
     1801 tatttgtgtt gacaaaaaaa aagaatcata tcaaatgaga tgttggttaa tgatgtgctt
     1861 ggtttttgtg ttattacaca taactcattg aatataatgg gttttttgtt gataatattt
     1921 tcatgttgaa ttgacttttg ggggataaat gtcagagaag gcaaaaaaag taggtaggcg
     1981 ctctctatca aaaagaagag aaagactaat aaaagagctt tggggggatg agctttccga
     2041 actgaatata tggaacagga aagaacacga tggatttaca acaatcccaa gaacattgaa
     2101 ttatatcaat aggataatcg attaccttgc tggcgctgga tctcctgtgt cacagacata
     2161 cctcagttta tggtgtcgag tttttgacga gggattcgtc gaaattaggg ataaagatgc
     2221 tctggcattt gagtcagggt ttagtgggca gcgagcggtg actacttggc taggaagaat
     2281 gaaaaagcta cgggagctgg gactgatatc aacgaagcct ggtacttctg gcgagtttca
     2341 ttatgtaatc atgttgaatc cactttacgt aattaaaaag tcatatgaat caaatggatg
     2401 gaaaagagat gagcgttaca acgctctatt ttctaggatg caggaagtgg gggcggagtg
     2461 ggaatgaacg acatgaaagc tgttcgtcac cgatataagc agagatatga ttttgctgcg
     2521 tcagaagtag tgggggcgtt tgatgaactt agaagtttgg attggagttt agaaatcgca
     2581 tctttaagtg acgtggaaat gtcaaagaga tggattgggg ctgttgatcc taacggtgtt
     2641 gcaagattac cacctaacgg tggatgggac tgggttaagc tctataacgg ctatttgaag
     2701 aaccctagcg cattcgtctg tgccattaaa tcagaaggca agctatgcgc tctgattggt
     2761 ggcggggttt ctgatagtaa agtggtaact aagctgcatt accttgaatc agccccttat
     2821 gacacggctc tttcaggata tgcactggat atttctgtta cgtatgttgt tactgtcgcg
     2881 aatgcgatgg gaagcgaata ctcagccatc tatgagccaa atagcaaagt cagagagata
     2941 gctataaatg attatggatt tagcgaggac aacttgtttg ggacaagtaa agatttcgct
     3001 ctgtatctac caatgagccg gatttaaccg gctcatttat cctaccagta cgccgtagag
     3061 tttttctggc tgttggtttg atcggccttg ctctgctcgt cttgctttta cgcgctggcg
     3121 aaatgctccg gctgtaccag aagagcatgg ttccgtcgct tttttaagtt cgaccgcgtg
     3181 agctaacata ttatttccgt tttttcgtga gttagtacaa agcatatgag atgacggata
     3241 cgtcaacttc atagtaactg tactgtattg tagaagagat gcgcaagtcg cagaattaat
     3301 gcgacccttc attgacgagg cgaagagtaa acgcgtatct tctttgaaaa cgaaaaaacc
     3361 acccggcaag gtggtttttc gaaggttcat taaaccggca agtttatgaa ccgtggtaaa
     3421 cagggtgtac aagaccactg ccaccaaatt cgtccttcca gtttagccgt agttgggctg
     3481 atacttcaag aactctgcgt tagtaatctc ttgtacatcc tcttaccgtt ggctgctgcc
     3541 agtggcgctt ttgcatgtct ttccgggttg gactcaagat gatagttacc ggataaggcg
     3601 cagcagtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgcctac
     3661 ccggaactga gtgtcaggcg tggaatgaga aaccgcggcc ataacagcgg agtgacaccg
     3721 gtaaaccgaa aggcaggaac aggagagcgc acgagggagc taccaggggg aaacgcctgg
     3781 gatctttat
//
