LOCUS       NZ_CP048328            10122 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain 61 plasmid p61_B, complete sequence.
ACCESSION   NZ_CP048328
VERSION     NZ_CP048328.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951923
            Assembly: GCF_010365405.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10122)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: 61.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010365405.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:29:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,708
            CDSs (total)                      :: 4,588
            Genes (coding)                    :: 4,381
            CDSs (with protein)               :: 4,381
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 71 of 207
            Pseudo Genes (incomplete)         :: 123 of 207
            Pseudo Genes (internal stop)      :: 60 of 207
            Pseudo Genes (multiple problems)  :: 40 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048328.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="61"
                     /isolation_source="environment"
                     /db_xref="taxon:562"
                     /plasmid="p61_B"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            1..957
                     /locus_tag="GZS14_RS23220"
                     /old_locus_tag="GZS14_23255"
     CDS             1..957
                     /locus_tag="GZS14_RS23220"
                     /old_locus_tag="GZS14_23255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001293214.1"
                     /translation="MSELVVFKANELAVSRYDLTEHETKLILFCVAKLNPTIENPTRDE
                     LTVKFSCSEYARTMGLSYENAWGRLNSATSDLFKRSVELIYPTGAVSKRIFNWTEYAEF
                     NREEQTVTLVFSSYIQPLLFHLKKFIKYNLEHVKAFENKYSMRIYEWLLKELSQRKTHR
                     GNIEISIKEFKFMLMLEKNYPLYAELNRWILKPVTNDLNTYSNMKLTIEKRGRPADTLI
                     FQVELDKQIDLVTELAKDPASKKEDKTIRLTPENRLHEGLKTTLHDALTAKIQLTSFEA
                     KFLSDMQSKYDLNGSFTWLTQKQRTTLEKILAKYGRI"
                     /name="replication initiation protein"
     gene            965..>1051
                     /locus_tag="GZS14_RS23815"
                     /pseudo=""
     CDS             965..>1051
                     /locus_tag="GZS14_RS23815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            1123..2296
                     /locus_tag="GZS14_RS23225"
                     /old_locus_tag="GZS14_23260"
                     /pseudo=""
     CDS             1123..2296
                     /locus_tag="GZS14_RS23225"
                     /old_locus_tag="GZS14_23260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612096.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(2558..3691)
                     /locus_tag="GZS14_RS23230"
                     /old_locus_tag="GZS14_23265"
     CDS             complement(2558..3691)
                     /locus_tag="GZS14_RS23230"
                     /old_locus_tag="GZS14_23265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="WP_001554610.1"
                     /translation="MKISIIGNTANAMILFRLDLIKTLTKKGISVYAFATDYNDSSKEI
                     IKKAGAIPVDYNLSRSGINLVGDLWNTYLLSKKLKKIKPDAILSFFSKPSIFGSLAGIF
                     SGVKNNNAMLEGLGFLFTEQPHGTPLKTKLLKNIQVLLYKIIFPHINSLILLNKDDYHD
                     LIDKYKIKLKSCHILGGIGLDMNHYCKSTPPTNEISFIFIARLLAEKGVNEFVAAAKKI
                     KKTHPNVEFIILGAIDKENPGGLSESDVDTLIKSGVISYPGFVSNVADWIEKSSVFVLP
                     SYYREGVPRSTQEAMAMGRPILTTNLPGCKETIIDGVNGYVVKKWSHEDLAEKMLKLIN
                     NPEKIISMGEESHKLARERFDANVNNVKLLKILGIPD"
                     /name="glycosyltransferase family 4 protein"
     gene            complement(3691..4500)
                     /locus_tag="GZS14_RS23235"
                     /old_locus_tag="GZS14_23270"
     CDS             complement(3691..4500)
                     /locus_tag="GZS14_RS23235"
                     /old_locus_tag="GZS14_23270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788525.1"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_000788525.1"
                     /translation="MKNHKVSIIIPCFNNEKTIERCVLSALKQTHRNIEIICINDGSSD
                     NSWNILKKLSSLYGNVFAFNNEDNSGPSFSRNKGVSLSTGHFLSFLDADDYWHPKKLEL
                     QLSFINDENLDFLGSTCSIGEKNNQEINQEIKKEHLKLKIISFNMMLFKNYFQTPAVIM
                     KRDIFIPFNENQRFSEDYMSWLVIVYNKKNKCGLIYGRDLVFLDKFNFGVSGLSGNLWL
                     MEKWELKNIFNFLLKGKIMAVPAILFSLIKYGRRCALTKKNKGKGNK"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(4497..5612)
                     /locus_tag="GZS14_RS23240"
                     /old_locus_tag="GZS14_23275"
     CDS             complement(4497..5612)
                     /locus_tag="GZS14_RS23240"
                     /old_locus_tag="GZS14_23275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162676512.1"
                     /translation="MGVNSDHVVTVYTLFSCTLLGFYWLIKRKSFIKRSHIFLLCLVVA
                     YLVINIFVYNHKSITLITSHAVYIVISMWFIFFFKRKPIHLSYLIALGLLFVSSFLLIK
                     LFQLGFGDWGRLTVPVFYDNSWSYFPLGYESSSDPNVLAFMLCIGSIYMFFTLPNKYTI
                     NIIFILSMMCSFLTLSRSSFLSFFISFLIVSVYSFFVLKKTQLRLLVLIMISLAMSFVV
                     MKNIPAVVTENNGASFVTEPNITERIVSKASNSDRIARLKNAIVEISNLKTMFIGNGIG
                     YSTLTQDPHNFYLSTIIDSGILILAIVMMVLTKPLLMVSRTPAKSNVILGCMFLTIYYM
                     FISLFYWQVRVLYFVIILLFTFVNISKKSEQ"
                     /name="hypothetical protein"
     gene            5784..7010
                     /locus_tag="GZS14_RS23245"
                     /old_locus_tag="GZS14_23280"
     CDS             5784..7010
                     /locus_tag="GZS14_RS23245"
                     /old_locus_tag="GZS14_23280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080803.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="WP_001080803.1"
                     /translation="MNRLFSNAFWLMASQIGGLLIPLIELPLLARRLGADEYGKVVYVL
                     AISIMASVFIEYGFNFSAVKKIAGRKDDKYISQISTDVFLAKIILSIIPIILALTLIYT
                     TSSGISIPNNWFVWIILFILSFGFTPFWYYISIGNIIFPAILDLLLRVTGLILIVIFVN
                     SPSDAKTTIAIQSMVGALNTLISTAVVFYLSGVGAINIKRALNEIKDGFSVFLYKSSQG
                     IMITISSSLLGFFGGAKSVGFFVPSEKVTKVITTLMITMLNVAYPHFVKNLSDSKNNRR
                     HIKKTILNISAILLFLSLIFSFSVFVFSDKIIFFVFGIEYERAIPLIKTIIWVIPLRSW
                     VLSLTILWFIPINKEKFITKITLVNIMLISFTACLFIYLFGTIGIVMSILLSEVITFVI
                     LTYFFFKEN"
                     /name="oligosaccharide flippase family protein"
     gene            complement(7212..7796)
                     /locus_tag="GZS14_RS23250"
                     /old_locus_tag="GZS14_23285"
     CDS             complement(7212..7796)
                     /locus_tag="GZS14_RS23250"
                     /old_locus_tag="GZS14_23285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039052609.1"
                     /translation="MSDEIKKIAALIGHQQAQEKRVAALIEEFQAESAKLSRQTAQLAQ
                     VIHELDSASGKMTDTIRRSVNAALTQVEKELKQTGLAQQKPAVNALNQVVTAANESVSA
                     MRREMSRYTWKSAIYIALTIFAVMGGCLWGMYYFIDSGYDRIAKMQRMEAVWQKKAPLA
                     DISTCDGKPCVKVDTRQSYGDKKDTYLIIKQ"
                     /name="hypothetical protein"
     gene            complement(7809..8903)
                     /locus_tag="GZS14_RS23255"
                     /old_locus_tag="GZS14_23290"
     CDS             complement(7809..8903)
                     /locus_tag="GZS14_RS23255"
                     /old_locus_tag="GZS14_23290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_000179383.1"
                     /translation="MASYHLSVKTGGKGKAASHADYIAREGKYAREKDNDLEHQESGNM
                     PAWAAHKPSEFWKAADTFERANGCTYREIEIALPREFTPAQRLELVRDFVQQEIGDRHA
                     YQFAIHNPRAAIEGGEQPHAHIMFSERLNDGIERDPQPYFKRANTKNPERGGAKKVRFG
                     ETPTERKAYLIAQRERWADLQNKHLERYQHIDRVDARSLKAQGIEREPERHFGAGQVRQ
                     FEPAQLHALMERREAERQAQHCRDECNSVIDVTTTLREALSERDNLTLKHEQTSEPQLK
                     ATSDKAFDFEKESDKLNALVSDAMKDIQEEIDLQTLVNDAMAEFQGIHQDMVKAEQQRQ
                     QEKERQRLAEQKRQKSDKGWSFSR"
                     /name="MobA/MobL family protein"
     gene            9069..9524
                     /locus_tag="GZS14_RS23260"
                     /old_locus_tag="GZS14_23295"
     CDS             9069..9524
                     /locus_tag="GZS14_RS23260"
                     /old_locus_tag="GZS14_23295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_000787081.1"
                     /translation="MKNEKQAKWLTDRVQTIRGLKSPNEPQRLLVILHDKADKTAQEIK
                     TLSLLIQAERAAEKAQEARTKVMNLIQAEKRAEAKAARKARDHALYQSAGLLIMAGLVD
                     SQTGKPVDDPAKLLGALVSLHELSRDNPKWEEWKLKGQVLLNQKKED"
                     /name="conjugal transfer protein TraD"
     Typing          2..957
                     /name="rep_cluster_1155"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.687"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7813..8903)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="85.984"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtctgaat tagttgtgtt taaagcaaat gaattagcag taagccgtta tgatctaact
       61 gaacatgaaa ccaagctaat tctgttttgc gttgcaaagt tgaaccctac aattgaaaac
      121 ccaacaaggg atgaattaac agttaaattc tcatgttctg aatatgcacg cactatgggc
      181 ttaagttatg aaaatgcttg gggaagattg aacagtgcaa ctagtgattt atttaaacgc
      241 tctgttgaat taatttatcc tacaggagca gtttctaaac gaattttcaa ttggacggaa
      301 tacgctgagt tcaatcgaga agaacaaact gtaacattag tatttagctc atatattcaa
      361 ccacttttat ttcatttaaa aaaattcatt aaatacaacc ttgagcatgt taaagccttt
      421 gaaaataaat actcaatgcg aatatatgaa tggttactaa aggagctttc acaaagaaaa
      481 acgcataggg gaaacattga gataagtatc aaggaattta agtttatgct catgcttgaa
      541 aaaaactacc ctttatatgc agaattgaat cgctggatcc taaaaccagt tacaaatgac
      601 ttaaacactt acagcaatat gaagttgact attgaaaaac gcggtcgtcc tgctgacaca
      661 ctgatctttc aagttgaact ggataaacaa attgaccttg tgactgaact agcaaaagat
      721 cccgcatcaa aaaaagaaga taagacaatc cgtttaacgc ctgaaaatcg tcttcatgag
      781 gggctaaaaa caacattgca tgatgcttta actgcaaaaa ttcaactgac tagttttgaa
      841 gcaaaattcc tgagcgatat gcaaagcaag tacgatctta acggctcatt tacatggcta
      901 actcaaaaac aacgaactac gctagagaaa attctggcta aatacgggcg gatatgaggt
      961 atctatggca ctggtatcag ttacagaggc agccagacta actggaaaaa acagagcaac
     1021 aatacatcgc tatattaagc aaggaaaatt gtagtggcat actgaatttg gccacctgaa
     1081 tagaggtgat atcatcactt cgcagtaaaa acaggtgaca ttatgaccgg acgtaacaga
     1141 cgcaatttta gccccgggtt tcgcctcgaa gctgcccagc ttttactcga tcagcattac
     1201 accgttgccg ccgctgctac ggcaatgaac gtcggcaaat ccacaatgga caaatgggtt
     1261 cgacaactga aagaagagcg agcgggaaaa tcccccacta cttcacccat gacacctgag
     1321 cagattgaaa tacgcgagct aaagaaaaga cttcaacgtg ttgaaatgga aagggatata
     1381 ttaaaaaatg ctaccgagac cttgatgtca gactccctaa acaattctca ttagttgaga
     1441 aactcagggc gcagtttcct gttgccattg tgtgcaacgt gtttggggtt catcgcagca
     1501 gttataaata ctggcggcag cacgggaagc ctgacgccac aagagtggca ttactgagcc
     1561 ttgtgcgtga agtttatcgc gaaagtaacg gctcagcagg agcgcgaagc attaccgcga
     1621 tggtcaccac caaaggtata aaactgagcc gctgacgggc aacaaagcta atgaaagcgc
     1681 tcaatattat cagctgtcag caacctgtcc atcgttataa gaaggcgtct aaggaacaca
     1741 ttgagatccc taattatctg gatcgccagt ttgctgttac cgagcctaat cacgcctggt
     1801 gcggtgatgt gacttatatc tggacgggaa aacgccgggc ttatctggtg tagtactcga
     1861 tttgttttcc cgtaaaccag ctggctgggc gatgtcattt ttccctgatt cagcactgac
     1921 agctaaagcc ctgtcgatgg cctgggaagc acggggaaaa ccggataatt tactgtatca
     1981 ctcggatcaa ggcagccact ataccagcag gaatttcagt cagttactgt ggagatatca
     2041 gataaagcaa agtctgagtc gccggggaaa ttgctgggat aacagcccaa tggaacggtt
     2101 cttcagaagc ctgaaaacag agtgggtacc ggataatggc tacgctaatt ttagccaagc
     2161 cagcatggca ataacgaatt acatcacagg atattacagc cagctcagac ctcatcaata
     2221 taatggtggt ttgacgccga atgaatcaga acgattgttc ttgaaaaact ctaaagccgt
     2281 ggccagtttt tgttgaccac tacacaatgt tgtattttgc atacagcata atcgactgga
     2341 ataaaattgt attggaatag gctctaacac tgatataacg gcagtttaga acctatctca
     2401 atagggatta attaccgcat aaaaatatat tatcactgta accaaaacct tatacagcct
     2461 gacttttaaa catcgatatg gctgggtaag gtcatgaatt tagctcctat tgagataggt
     2521 tcttagttcc gaatgaatca gagccgcttt tcgtttatta atcaggaatt cctagtattt
     2581 ttaataactt tacattattt acatttgcat cgaatctttc tcttgctaac ttatgacttt
     2641 cttctcccat actgattatt ttttcaggat tattaattaa cttcagcatt ttttctgcaa
     2701 gatcttcatg tgaccatttt tttacaacat atccattcac accatcaata attgtttctt
     2761 tgcagcctgg taaattagta gttaaaatcg gtctccccat agccatcgct tcttgtgtac
     2821 tacgaggaac tccctctcga taataggaag gaagaacaaa tacgcttgat ttttcaatcc
     2881 aatcagccac attagaaaca aatccgggat aagaaataac tcctgattta attaaagtat
     2941 ctacgtcaga ttcagataac cctccggggt tttccttatc tatagcgcca agtataataa
     3001 attcaacatt gggatgtgtt ttttttattt tttttgcggc agcaacaaac tcattgactc
     3061 ctttttctgc tagcaaacga gctataaaaa tgaatgatat ttcatttgtt ggtggcgtgc
     3121 ttttacagta atgattcata tctaaaccaa tgccaccaag aatatggcaa gattttaatt
     3181 ttattttgta tttatctatc aagtcatgat aatcatcctt gttaagaagt attaatgagt
     3241 tgatatgtgg aaatattatt ttatacagga gaacctggat atttttaagt aactttgttt
     3301 ttaacggagt tccatgtggc tgctctgtaa ataaaaaacc taacccctca agcatagcgt
     3361 tattattttt aacgcctgaa aaaataccag ccaacgatcc aaagatagag ggctttgaaa
     3421 aaaaagataa aatagcatct ggttttatct tctttagttt tttacttaat aagtaagtat
     3481 tccataaatc accaacaagg ttaataccac tgcgacttaa attataatca acaggaatgg
     3541 cgcctgcttt ttttattatt tccttggatg aatcattata gtcagtagca aaagcataga
     3601 ctgaaatccc tttcttggtt agtgttttta ttaaatccaa tctaaacaaa atcatagcat
     3661 ttgctgtgtt ccctattatt gagatcttca ttatttatta cccttacctt tatttttctt
     3721 tgttaaagcg catcttcttc catattttat caaagaaaac aagatcgcag gcactgccat
     3781 tattttacct ttcaacaaga agttaaatat attttttaac tcccacttct ccatcaacca
     3841 taaattacca ctcaaccctg acactccaaa gttaaattta tcgagaaaaa ccaaatccct
     3901 tccatatatt aatccacatt tgtttttttt attataaacg ataacaagcc atgacatgta
     3961 gtcctctgaa aaacgctgat tctcattaaa tggaataaaa atatctcttt tcataatgac
     4021 ggctggagtc tggaaataat tcttaaacaa catcatgtta aatgaaatta tttttaattt
     4081 taaatgttct tttttaattt cttggttaat ttcttggtta tttttctcac caatggaaca
     4141 cgttgaacct aaaaaatcca agttttcatc attaataaat gatagttgta attctagttt
     4201 ttttggatgc cagtaatcat ctgcatccaa aaatgacaaa aaatgccccg tggatagtga
     4261 aaccccttta ttcctggaaa atgacggccc tgaattatcc tcattattaa aagcaaaaac
     4321 atttccatac aatgagctta atttctttaa aatattccag ctattatctg acgatccatc
     4381 atttatacaa ataatctcta tatttctatg ggtttgtttt aatgcggaaa gaacgcatct
     4441 ttctatagtt ttttcgttat taaaacaggg gatgataata ctaaccttat ggtttttcat
     4501 tgctcactct tcttacttat atttacaaag gtaaataata atataataac aaagtatagg
     4561 actctcactt gccagtagaa cagagaaatg aacatatagt atatagtcaa gaacatacac
     4621 cccaaaataa catttgattt tgcgggggtt cttgaaacca ttaataatgg ttttgttagc
     4681 accatcatta caattgctaa tatcaaaata ccggaatcaa taatagttga taagtaaaaa
     4741 ttatgtggat cctgtgttaa tgtactatag ccaataccat taccaataaa cattgtcttt
     4801 agattactta tttcaacaat agcatttttt aatctggcta ttctatcact attacttgct
     4861 ttactaacta ttcgttctgt tatatttggt tctgtgacaa atgaagctcc attattctca
     4921 gtcactacag cgggaatgtt tttcatcact acaaaagaca ttgcaagtga aatcataatt
     4981 agtaccaaca accttagctg tgttttcttt agcacaaaga atgaataaac acttacaata
     5041 agaaaggaaa taaaaaaaga taaaaaagaa ctccgtgata aggtcaaaaa tgaacacatc
     5101 attgacaata tgaatataat attaattgtg tatttatttg gcaatgtgaa aaacatatag
     5161 attgagccaa tgcataacat aaaagctaac acatttgggt cacttgaact ttcataaccc
     5221 aaaggaaaat aagaccatga gttatcataa aaaacaggaa ccgttaaacg tccccagtca
     5281 ccaaagccaa gttgaaataa tttaatcaac aaaaaagagc taacaaaaag caggcctaaa
     5341 gcaattaaat atgataaatg tattggtttt cttttaaaga agaaaataaa ccacatagat
     5401 attactatat atacagcatg actagtaatt agagtgatag atttatgatt atatacaaaa
     5461 atgttaatga caaggtaagc aactactaag cacaggagaa aaatatgtga tctttttata
     5521 aaagactttc tttttataag ccaatagaaa cctaatagtg tacaggaaaa gagagtgtat
     5581 actgtaacca catgatcact attgactccc atgaccacct gtttattccc tcccataact
     5641 gaccacaata taaaaggaaa agtgcaaatg tatagataaa aacacatagt gattcaactg
     5701 gttattttta ttgtaaataa gattctttta taatatcata aacatgatgc ctcactcaac
     5761 atttttaaca gatgatattt tttatgaaca gacttttttc taatgctttc tggttaatgg
     5821 catctcagat tggcggcctt ctaatcccct tgatcgaact gccattgctt gccagaagac
     5881 tgggggctga tgaatatgga aaagttgtat atgttttagc tatttccata atggcttctg
     5941 ttttcattga gtatggattc aatttcagtg ctgtgaaaaa aatcgcagga aggaaagatg
     6001 ataaatatat ttcacaaatt tcaacagatg tttttcttgc taagataatt cttagtatta
     6061 tacctattat tttagctctc accctaatat acacaacatc tagcggaata tctataccta
     6121 ataattggtt tgtatggatt attttattta tcttatcttt tggattcact ccgttctggt
     6181 attacatatc aataggaaat ataattttcc ccgcaatact cgatctttta ctaagagtga
     6241 caggtttaat tctcattgtt atttttgtta actccccgag tgacgcaaaa accacaatag
     6301 ccattcaatc aatggtagga gcgctaaata cattaatatc aactgctgtt gttttttatt
     6361 tgtcgggagt aggagcaatt aatattaaaa gagcgcttaa tgaaattaaa gatgggttta
     6421 gtgtattttt gtataaaagt tcacaaggaa taatgataac catatcatca tcattattag
     6481 gtttttttgg gggggctaaa tctgttggtt tttttgttcc atctgaaaaa gtaactaagg
     6541 ttataacaac attaatgata acaatgctaa acgtggcata tccgcatttc gttaaaaatc
     6601 ttagtgatag caaaaacaat agacgccaca taaaaaaaac aatccttaac atatcagcaa
     6661 tattattatt tttatcatta attttttctt tttctgtttt tgttttctcc gataaaataa
     6721 ttttttttgt ttttggtatt gaatacgaaa gagcgatacc attaattaag actattattt
     6781 gggttatacc gttaaggtca tgggtgttgt cacttacaat tctatggttc attccaataa
     6841 ataaagagaa atttatcaca aaaataacac tcgttaatat aatgctaatt tcgttcactg
     6901 cgtgtttatt tatttatctg tttggcacta taggaatagt gatgtcaatt ttactatcag
     6961 aagtaataac ttttgtgata ttgacctatt tctttttcaa agaaaactaa gagcctatcc
     7021 caaaaggaat tatcgtataa tataatgaat tacgataatt tctgagggca acgtatggca
     7081 gacaacaagt ggcaaatcag tgatagactc tgggagaaaa tggctcctct aatcctaacc
     7141 atgaaccgct caaaaccacc tcgctacttt gacgacttgc acgggaaaac ggcgtattca
     7201 gcgccgtttt tttattgttt gataatcaga taggtgtctt ttttatcacc atatgactgc
     7261 cttgtatcca ctttcacaca aggttttccg tcacacgtgg agatatcagc cagcggggct
     7321 tttttttgcc agaccgcttc catgcgctgc atttttgcaa ttcggtcata accgctgtcg
     7381 ataaaataat acattcccca aagacacccg cccatcaccg caaatatcgt caatgcgata
     7441 taaatggcag acttccacgt ataacgggac atttcacgtc gcattgcact cacggattca
     7501 ttagctgctg taaccacctg attcagcgca ttgacggcgg gtttctgctg cgccaatccc
     7561 gtttgtttga gttctttttc aacctgagtt aaggctgcat tcaccgatcg cctgatcgtg
     7621 tcggtcatct taccggaggc actgtccaat tcatggatca cctgagccaa ttgtgctgtt
     7681 tggcggctta atttggcgga ttccgcctga aattcttcaa tcaacgccgc cacacgcttt
     7741 tcctgcgctt gctgatgccc aatcagcgcc gcaatttttt taatctcatc actcatacaa
     7801 aatcctgttt atcgggaaaa tgaccagcct ttatcggact tttgtcgttt ctgctcggcg
     7861 agtcgctgcc gttctttttc ctgctgacgc tgttgctcag ccttaaccat gtcctgatga
     7921 atgccctgaa actccgccat cgcatcgtta acaagggtct gaagatcgat ttcttcctgt
     7981 atgtccttca tggcatcact gaccagtgcg ttcaacttgt cagactcttt ttcaaaatca
     8041 aacgctttgt ctgaagtcgc tttcaactga ggctctgacg tctgctcgtg tttcagtgtc
     8101 agattatcac gctcgctgag ggcttcccgt agcgtggtgg taacatcaat cacgctgtta
     8161 cattcatcac gacaatgctg agcctgtcgc tccgcttctc tgcgctccat cagggcgtgt
     8221 aactgcgcgg gttcaaactg cctgacctgt cctgcgccaa aatgtcgctc cggctcacgc
     8281 tcaatcccct gtgctttcag ggaacgcgca tcaacgcggt cgatatgctg ataccgttca
     8341 agatgcttat tctgtaagtc agcccagcgt tcccgctggg cgataaggta tgccttgcgt
     8401 tcggtcgggg tttcgccgaa ccgcaccttt tttgcgccgc cccgttcagg gtttttcgtg
     8461 ttagcccgtt taaaataggg ctgcgggtca cgctcgatac cgtcattaag ccgttcagaa
     8521 aacataatat gcgcgtgcgg ttgttcaccg ccttcaatcg ccgctctggg gttatgaatg
     8581 gcaaattgat aagcgtgccg gtcgccaatt tcctgttgaa caaaatcgcg aaccagttca
     8641 aggcgttgtg cgggtgtaaa ttcacgcggc agggcaattt caatttcgcg gtaggtacag
     8701 ccgttggcac gttcaaacgt gtcagcggct ttccaaaact cggacggttt atgcgctgcc
     8761 catgcgggca tattaccgga ctcttgatgc tcaaggtcat tatctttttc acgggcatat
     8821 tttccctcac gcgcaatata atcggcatgg gaagcggctt tgcctttccc gccggtttta
     8881 acgctgagat gataagatgc catgctgttt tcgcccgctg caagcgcgca ccgtttctga
     8941 acggagtgaa gaaacgtcta agtgcgccct atcaataaaa aggatgatag ggattgtagt
     9001 ggaatttgac catagttgcc aatcatgagc ataattattt cattctaaaa acagcatgag
     9061 tggtaaagat gaaaaacgaa aagcaagcaa aatggctcac agacagagtg caaactatcc
     9121 gtggtttaaa atcaccgaat gagccacaac gcttattggt gattttgcac gataaagcgg
     9181 ataaaaccgc ccaagaaata aaaacgctct cattgttaat tcaggcggag cgggcagcag
     9241 aaaaagccca ggaagcccgc acaaaggtaa tgaatcttat tcaagcagag aagcgtgcag
     9301 aagccaaagc tgcccgtaaa gcacgtgacc atgcccttta tcaatcagcg gggttactca
     9361 tcatggcggg gttagtggac agtcaaacgg gtaaaccggt agatgacccc gcaaaactac
     9421 tgggggcatt agtcagcttg catgaattat cccgtgataa tcccaaatgg gaagaatgga
     9481 aattgaaggg gcaggtatta ctcaatcaga aaaaggaaga ttagagcaca gtgttaatac
     9541 cctctaaaac gccacagaaa gtccgtggcg aggttttgtt ggtttgataa tggaatttat
     9601 cacctaatac tgaaaacatg gcacagaagc gcaaacaacc accttcctga ttgagccgtg
     9661 agcgaagcga acccaatgac acgaaaaaaa cagcggcgct gtggctggat ggtcggagac
     9721 aaaagaaagg ggcagcgcgt tgcccgagca aagcgaggga gctacttagg tgttttaggt
     9781 ttgtcttgaa aaggagcaaa cgttgtttgc gacatccttt taactacggc aaatcgacgg
     9841 caattttctg atttatccac agggctggga tctattcttt ttttatcttt tatttattct
     9901 ttctttattc taataatttt aacttattgt tttttaataa aaaaaaaacc attaaatgta
     9961 ggaaaaatta ggctagatgt aggaaaaatt aggctagcag tgggaaagat gtaggaaaaa
    10021 ttaggttatt cacaatcaaa cccgaaaaaa tgtagttgat ttaaatcgct acatcattat
    10081 gattcatgta gcgatttaaa ttactacata atggtgatta gc
//
