LOCUS       NZ_CP048324             3789 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain 64 plasmid p64-B, complete sequence.
ACCESSION   NZ_CP048324
VERSION     NZ_CP048324.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951924
            Assembly: GCF_010365365.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: 64.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010365365.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:46:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,378
            CDSs (with protein)               :: 4,378
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 74 of 207
            Pseudo Genes (incomplete)         :: 123 of 207
            Pseudo Genes (internal stop)      :: 57 of 207
            Pseudo Genes (multiple problems)  :: 40 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048324.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="64"
                     /isolation_source="environment"
                     /db_xref="taxon:562"
                     /plasmid="p64-B"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            338..856
                     /locus_tag="GZS15_RS22955"
                     /old_locus_tag="GZS15_22985"
     CDS             338..856
                     /locus_tag="GZS15_RS22955"
                     /old_locus_tag="GZS15_22985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162676513.1"
                     /translation="MSEKAKKVGRRSLSKRRERLIKELWGDELSELNIWNRKEHDGFTT
                     IPRTLNYINRIIDYLAGAGSPVSQTYLSLWCRVFDEGFVEIRDKDALAFESGFSGQRAV
                     TTWLGRMKKLRELGLISTKPGTSGEFHYVIMLNPLYVIKKSYESNGWKRDERYNALFSR
                     MQEVGAEWE"
                     /name="hypothetical protein"
     gene            853..1416
                     /locus_tag="GZS15_RS22960"
                     /old_locus_tag="GZS15_22990"
     CDS             853..1416
                     /locus_tag="GZS15_RS22960"
                     /old_locus_tag="GZS15_22990"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000999222.1"
                     /translation="MNDMKAVRHRYKQRYDFAASEVVGAFDELRSLDWSLEIASLSDVE
                     MSKRWIGAVDPNGVARLPPNGGWDWVKLYNGYLKNPSAFVCAIKSEGKLCALIGGGVSD
                     SKVVTKLHYLESAPYDTALSGYALDISVTYVVTVANAMGSEYSAIYEPNSKVREIAIND
                     YGFSEDNLFGTSKDFALYLPMSRI"
                     /name="hypothetical protein"
     gene            complement(1426..1578)
                     /locus_tag="GZS15_RS22965"
                     /old_locus_tag="GZS15_22995"
     CDS             complement(1426..1578)
                     /locus_tag="GZS15_RS22965"
                     /old_locus_tag="GZS15_22995"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001341064.1"
                     /translation="MLAHAVELKKATEPCSSGTAGAFRQRVKARRAEQGRSNQQPEKLY
                     GVLVG"
                     /name="hypothetical protein"
     gene            complement(1875..2021)
                     /locus_tag="GZS15_RS23810"
     CDS             complement(1875..2021)
                     /locus_tag="GZS15_RS23810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050863271.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPERHAKAPLAAANGKRMYKRLLT
                     QSS"
                     /name="hypothetical protein"
     gene            2581..2730
                     /locus_tag="GZS15_RS23450"
     CDS             2581..2730
                     /locus_tag="GZS15_RS23450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_180251257.1"
                     /translation="MPSEHDEQRDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     Typing          2374..2588
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.52"
                     /coverage="100.0"
                     /evalue="3.460000000000001e-56"
     Typing          complement(1913..2410)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.258"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 gcccgcttat gtgggctttt tcattgcgca ttcatggcac cccttcctcc ccccttaaac
       61 cccctaagaa ccccattcag cacagcgagc acaacgatta cattagagta cattgagaac
      121 agcgagtaca aaataacgca taactaattg aaaaataata ccgtaaccgt aaagaatatt
      181 acatctcaat atttgtgttg acaaaaaaaa agaatcatat caaatgagat gttggttaat
      241 gatgtgcttg gtttttgtgt tattacacat aactcattga atataatggg ttttttgttg
      301 ataatatttt catgttgaat tgacttttgg gggataaatg tcagagaagg caaaaaaagt
      361 aggtaggcgc tctctatcaa aaagaagaga aagactaata aaagagcttt ggggggatga
      421 gctttccgaa ctgaatatat ggaacaggaa agaacacgat ggatttacaa caatcccaag
      481 aacattgaat tatatcaata ggataatcga ttaccttgct ggcgctggat ctcctgtgtc
      541 acagacatac ctcagtttat ggtgtcgagt ttttgacgag ggattcgtcg aaattaggga
      601 taaagatgct ctggcatttg agtcagggtt tagtgggcag cgagcggtga ctacttggct
      661 aggaagaatg aaaaagctac gggagctggg actgatatca acgaagcctg gtacttctgg
      721 cgagtttcat tatgtaatca tgttgaatcc actttacgta attaaaaagt catatgaatc
      781 aaatggatgg aaaagagatg agcgttacaa cgctctattt tctaggatgc aggaagtggg
      841 ggcggagtgg gaatgaacga catgaaagct gttcgtcacc gatataagca gagatatgat
      901 tttgctgcgt cagaagtagt gggggcgttt gatgaactta gaagtttgga ttggagttta
      961 gaaatcgcat ctttaagtga cgtggaaatg tcaaagagat ggattggggc tgttgatcct
     1021 aacggtgttg caagattacc acctaacggt ggatgggact gggttaagct ctataacggc
     1081 tatttgaaga accctagcgc attcgtctgt gccattaaat cagaaggcaa gctatgcgct
     1141 ctgattggtg gcggggtttc tgatagtaaa gtggtaacta agctgcatta ccttgaatca
     1201 gccccttatg acacggctct ttcaggatat gcactggata tttctgttac gtatgttgtt
     1261 actgtcgcga atgcgatggg aagcgaatac tcagccatct atgagccaaa tagcaaagtc
     1321 agagagatag ctataaatga ttatggattt agcgaggaca acttgtttgg gacaagtaaa
     1381 gatttcgctc tgtatctacc aatgagccgg atttaaccgg ctcatttatc ctaccagtac
     1441 gccgtagagt ttttctggct gttggtttga tcggccttgc tctgctcgtc ttgcttttac
     1501 gcgctggcga aatgctccgg ctgtaccaga agagcatggt tccgtcgctt ttttaagttc
     1561 gaccgcgtga gctaacatat tatttccgtt ttttcgtgag ttagtacaaa gcatatgaga
     1621 tgacggatac gtcaacttca tagtaactgt actgtattgt agaagagatg cgcaagtcgc
     1681 agaattaatg cgacccttca ttgacgaggc gaagagtaaa cgcgtatctt ctttgaaaac
     1741 gaaaaaacca cccggcaagg tggtttttcg aaggttcatt aaaccggcaa gtttatgaac
     1801 cgtggtaaac agggtgtaca agaccactgc caccaaattc gtccttccag tttagccgta
     1861 gttgggctga tacttcaaga actctgcgtt agtaatctct tgtacatcct cttaccgttg
     1921 gctgctgcca gtggcgcttt tgcatgtctt tccgggttgg actcaagatg atagttaccg
     1981 gataaggcgc agcagtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga
     2041 actgcctacc cggaactgag tgtcaggcgt ggaatgagaa accgcggcca taacagcgga
     2101 gtgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagct accaggggga
     2161 aacgcctggg atctttatag tcctgtcggg tttcgccgcc actgatttga gcgtcagatt
     2221 ttgtgatgct tgtcaggggg gcggagccta tggaaaaacg gcaacgccgc atcctttttc
     2281 cgctcttcct gatttccgct aagtctcttt atccagccat tcgtgagctg gcaacgcccg
     2341 ccgcagtctc acggcaggag cgcagcgacc gagtgagcga ggaggcgtca tttcaccggg
     2401 atctgcattt cgcacttaca caccggtgct gcatttcccg gtaccacatg aagcactttc
     2461 gtaaattatt actgcgttgt agcattttaa gcccgtatac actccgctag cgctacgtga
     2521 ctggttcagg gcttcgcccc gaaacccgct aacaaccttt cagctactgg atatcacccc
     2581 atgccatctg aacatgatga acaacgggat tcgactgata cgcagcttcc cggcgaatat
     2641 gacctttttg atttaatggc gcaggttact gaagagaacc tgcacgcccc ggtggatttt
     2701 ggcccgcctg tcggcaggga agcgttataa cttttttcgc tggtcagcgt acgtgtaata
     2761 cgccggtatt acatcttatt tctttatttt catgtgatta caggtggttg taatacgttg
     2821 cgtattacat aaataaacgg gaaattcagt ttttaccttt taattcatgc aattgcgatg
     2881 aaaatcgcgg ttgcgtatgg tgtatgacca tttaagggat aaaaaatcat cttttttggt
     2941 agggcgctct ccgtaggtta aggggtattt ggctaaaaag cgtcctattc tttgatggtc
     3001 atgcaagcat gaccatctga gcaaccaaaa actacagata aactacaaaa aacgttttgc
     3061 attagtgatg gcagactaca aaaaaactac aaaaaccgtg gcggactact aatagactac
     3121 aagaaaacta cagataaact acaaagtaac taccggatta cctcagatcc gttccctgaa
     3181 ccattcgtac atcggccccc ttcctgaacg tgctgaaaac gggttttccg gctgtgccgg
     3241 caacgcctca cggccacgca ataaattgcg tccccgttca ccgttttcgg gtaagaagaa
     3301 tatcggaaac aggacttacc ccggctgaat ggcgtgaatt tccgcgctat gcacttgcgc
     3361 gcatactcat gcatgccgta aaaacagagc ctgcgcgttt ctggcgggtt ttcgggtggt
     3421 ttgttgcctg ttttaccggt ttcccgtcag aaacgccctg agggcctctc aggcggtgca
     3481 cgcaagagat gttatggtaa atggtttctg aggtgtaagc gaaaactcga gttttgacgc
     3541 cattctgagt aggtaatttg cagacggaat aaatactggc gagcaataag ctcatcaaca
     3601 aaaaatggag atcctaagat ggatagagaa accctatacc tactcaaaac gttagaccat
     3661 aacaatgcct tgcttgacga tattatccga agaaagatgg gtaggtacta caacacgaag
     3721 attttgcgtg attcctgtgg tgctattgaa gccgaactac gacgtcgagg aatcatttaa
     3781 aaagacgaa
//
