LOCUS       NZ_CP048320             1822 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain 32-4 plasmid p32-4_J, complete sequence.
ACCESSION   NZ_CP048320
VERSION     NZ_CP048320.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951925
            Assembly: GCF_010365345.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: 32-4.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010365345.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:42:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,109
            CDSs (total)                      :: 4,987
            Genes (coding)                    :: 4,642
            CDSs (with protein)               :: 4,642
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 345
            CDSs (without protein)            :: 345
            Pseudo Genes (ambiguous residues) :: 0 of 345
            Pseudo Genes (frameshifted)       :: 151 of 345
            Pseudo Genes (incomplete)         :: 215 of 345
            Pseudo Genes (internal stop)      :: 53 of 345
            Pseudo Genes (multiple problems)  :: 66 of 345
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048320.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="32-4"
                     /isolation_source="environment"
                     /db_xref="taxon:562"
                     /plasmid="p32-4_J"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            complement(128..415)
                     /locus_tag="GZS16_RS24785"
                     /old_locus_tag="GZS16_24785"
     CDS             complement(128..415)
                     /locus_tag="GZS16_RS24785"
                     /old_locus_tag="GZS16_24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023909281.1"
                     /translation="MRIKYESRWKLEELKLEICRSIDSLIDEYHIEYAMGINLYMNCED
                     KEFNPVDIVNEDGKNIMFVVSNNEKIQKPKKSAKKKIPAPVIELDKYRKK"
                     /name="hypothetical protein"
     gene            complement(428..961)
                     /locus_tag="GZS16_RS24790"
                     /old_locus_tag="GZS16_24790"
     CDS             complement(428..961)
                     /locus_tag="GZS16_RS24790"
                     /old_locus_tag="GZS16_24790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005707163.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000893324.1"
                     /translation="MLEQQFEDFEQQKRRDHQAQDQSKSAKQKRDRLNPPDWSQVTKAG
                     WNMLDSISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDLES
                     IGAIVKIPVGIYYAYALDPYQVWRGYNKSKKYAAFNAKTLTQKDGVIDRKLRLMLSRRE
                     EFEGSLKTSNHDLD"
                     /name="replication/maintenance protein RepL"
     Typing          1448..1699
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.49"
                     /coverage="97.0"
                     /evalue="8.62e-98"
ORIGIN
        1 aaaacgggga gcaaaaagac gcaagggatt ttgaggcggc aaaagagcaa tgcaggatag
       61 acaatccact agaaattgca tgcaatgttc tgtggattgt ccgcctgccg ctggctctgt
      121 atggccatca ctttttccga tatttatcta actcaattac aggtgcagga atttttttct
      181 tcgcactttt ttttggtttt tgtatttttt cattgttgct aacgacaaac ataatattct
      241 ttccatcttc attgacaata tcaacgggat taaactcctt atcttcacaa ttcatatata
      301 gatttattcc cattgcatat tcgatatgat attcatcaat taacgaatct attgatctac
      361 atatttctaa ttttagttct tctaatttcc aacgtgattc atatttaatt ctcatagcgc
      421 atcataccta gtccaagtca tgattagatg tcttaagcga gccttcaaac tcttctctcc
      481 tagagagcat gagccgcaac ttgcgatcaa taacgccatc tttttgagtt aatgtttttg
      541 cgttaaacgc tgcatatttt ttagatttat tatatccgcg ccatacctga tacggatcta
      601 acgcataagc ataatagata cctactggta ttttcacgat ggcaccaata ctttctaaat
      661 ctccaatcca atattggatc gtccttaaac taacaccgaa ataatcagct aagaattttt
      721 ggtcacatac aacagcccca cacgatttat ctatattttg agcaaaaaaa gaatatagcc
      781 taaatgctct gggaaccttc tcagatatag agtccaacat attccagccc gccttagtaa
      841 cctgcgacca gtcaggtgga ttcaaccgat ctcgtttttg cttagcactt ttggattgat
      901 cttgtgcctg atgatctcgc cgtttttgct gttcgaaatc ttcaaattgc tgttccagca
      961 tacttgcatc cttacctgca acgttgtttc gtaccttacg aaatttatat cgtacattac
     1021 gaaacaacac acgcaaacat gtttgcgtat acacatgcaa ataacgtttc gtatgttggt
     1081 gtcataatgc tttgattttt aagagaatgt cgatttttgc tctaaagatc tatcaaagca
     1141 cttttttgct acacaagatt taattagttc atcgaacatt tggggtgtcg gggcgaagcc
     1201 ctgacccagt cacgtagcga tagcggagtg tatactggct tactcttctg caagcctcgt
     1261 cagccgtgat tttgcccgat ttcgcgctct ccacgttcaa cagctcgatc atgtttttgc
     1321 agtccgttca ccgctaaaac gccccgcatc agtgcgtacg tgctcaatgt ggtttccggt
     1381 tctcttccgc ttcctcgccc actcgctcac taacgttcgg tcgctggggc tgcggcgagc
     1441 ggtgccacct ctcgcatgtt atcggtgatt ccgctgttgt tctttcgggg atgagtcgcc
     1501 agatagctct tcaaacgctc tcagaagctc tcagacgcac gaaacgggtt ttaaatgccc
     1561 atcagggcat ggaaggcgcg aaaaacgctt acagagggtt ttaggaggtc aggcgcgccg
     1621 atacccctaa ccaggggatt tcaggtcgcc aggaaccgcg ccagccccct gcaaagccca
     1681 tttgcacgga ataggcagcc acgcggttgg taggtggtcg tagaccgtcg atgttacagc
     1741 gatgggggtc tggggttctc cccagacggt ttccggttgc gctcaaggcg ctgccggaac
     1801 cgtggcggct cggtcgttct gg
//
