LOCUS       NZ_CP048319             2101 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain 32-4 plasmid p32-4_I, complete sequence.
ACCESSION   NZ_CP048319
VERSION     NZ_CP048319.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951925
            Assembly: GCF_010365345.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: 32-4.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010365345.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:42:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,109
            CDSs (total)                      :: 4,987
            Genes (coding)                    :: 4,642
            CDSs (with protein)               :: 4,642
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 345
            CDSs (without protein)            :: 345
            Pseudo Genes (ambiguous residues) :: 0 of 345
            Pseudo Genes (frameshifted)       :: 151 of 345
            Pseudo Genes (incomplete)         :: 215 of 345
            Pseudo Genes (internal stop)      :: 53 of 345
            Pseudo Genes (multiple problems)  :: 66 of 345
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048319.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="32-4"
                     /isolation_source="environment"
                     /db_xref="taxon:562"
                     /plasmid="p32-4_I"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            complement(600..866)
                     /locus_tag="GZS16_RS24775"
                     /old_locus_tag="GZS16_24775"
     CDS             complement(600..866)
                     /locus_tag="GZS16_RS24775"
                     /old_locus_tag="GZS16_24775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(870..1835)
                     /locus_tag="GZS16_RS24780"
                     /old_locus_tag="GZS16_24780"
     CDS             complement(870..1835)
                     /locus_tag="GZS16_RS24780"
                     /old_locus_tag="GZS16_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(1211..1442)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 tacaaggatc acgcgcgaga attgcgcaaa aaataaccac aattttaacc ccccaaaaca
       61 agcacccccg aacggcttac cccaaccttt tccacacacc cgatcaccaa aacagggaat
      121 aaaaacaaaa ccctgtctta caggtggatg gcctcacttc gtgaaaccat ccaccccgca
      181 taagtttttt aatcggtggt gtcgtcaatc actaaatcgg aaccccaaag gggaccccca
      241 cgatttagca tttgacgaga ttaactctcg gagatggcat catgctttta acggacttta
      301 aaaaacggtg aacggatgcg gattttaatc cgtgtctgtg aggcgttgag caacgagcaa
      361 agcgaggcgg tagaaagaga gccagcggca ggctcaggac gataatccgg ggaaaatata
      421 ccactcgcag agtgttaaca tttgacatcg gattgtctat cctgcattgg tgtcgagttg
      481 gcaaggctga ggataatgct gaggataatg ctgaggataa tgcaggatag acagagcact
      541 agagattgca tgcaatctcg tacctatcac ctcagcctgc cgctggctct tttccagaac
      601 taagaatgcg ttcttaaaaa tccaaacttg ataccgttta aatctatttt cacaaaaaga
      661 gtttttaccc tctcgtttgt tttctgaata tataaaatta attcatcctc aaaaccttcg
      721 tgcggatgaa aaccaaatgt gccaaaactt aatccatcaa taagcaaaat aaggttttta
      781 aacctgtcag gaatcaccgc ttcacttctt aaaaaaaata aaccaatagt cttatttcca
      841 ttcaggtatt caatattaat attcatacgt taatccgatt tatccttctc aggggcgcgt
      901 ttgtaacgcc gtttacctga atcccagaca aacgccgtcc gcttcccgtc atcagtgcca
      961 tcccctacat cgtcggcaat gacaagatcc tcattggttt ctcgatccaa ctggaggacg
     1021 ttttttagcg ccccaccggt cgagataaaa cggcgcttgt gaagctgccg cgtcagctca
     1081 agaaaccact caggatcgtt tgccatatct tccggtttaa cggagtattt cagcgtttca
     1141 gcaaccgcgc tttgcagttg ctcggcaacg ttggccacaa cctcacctgt cttagttttt
     1201 actgcccgaa tatcgatatt cggctcatag ttcacccgca agcaatcgcg ccagagttct
     1261 acccaacgtt cgtgcttaac gtagttcttc cctttaaacc aagaaggttg caccatcagc
     1321 agacagtgaa aatgcggatg tgcgctgcca tccttacctc gcgtcacctc cgtagccctg
     1381 atccacccct gaacaggtga tagctccttt cgcttttcca ttcgcttaaa cgccgcattc
     1441 attgctgtaa ggactgttcc aagttcacct atctcgcagt tcctgacagt taacgtcaga
     1501 aacaaccatc gggaagacgg gtaatccaca acgattttcg gtagagcctg ataaaaacgg
     1561 gcttgccaca tgagggttct tctccactgg cagacagggc aatgacggac acggcaaaat
     1621 tgcgcactac gaagccgtag gcgcgtttct ccggtttcca tgatggtaga ccaaccaaaa
     1681 cgtaacaaac cgctacagga ggccatacgg agtgcatagc gctcgaattc agcagcagtc
     1741 tggtagatac ccccgacaga atcggtaagc gtgcgctggg tatcccaaac tgcatcacgg
     1801 gggctgtagt ccgaaaggaa tttatcttct gacattaagc ctgctccgat ctgggcaggc
     1861 tgtgatttga tgtggtatat tcatcacgta cctgtgcatt gcgagccagt acatgtcacc
     1921 agagtttgcc gctgaggtga cacattcgaa atgttgggga gtgttgacgc actccccaac
     1981 gccgattaaa aacgcctcaa ttctgctcca ttttctttga cctgtcaaaa cccactcaaa
     2041 gcctcgtcat tactgggcta aaccactatt tcatatccgc catttatccc ttaagtataa
     2101 a
//
